Effects of hemolysis and storage on quantification of hormones in blood samples from dogs, cattle, and horses.
Abstract: Veterinary diagnostic endocrinology laboratories frequently receive hemolyzed plasma, serum, or blood samples for hormone analyses. However, except for the previously reported harm done by hemolysis to canine insulin, effects of hemolysis on quantification of other clinically important hormones are unknown. Therefore, these studies were designed to evaluate effects of hemolysis on radioimmunoassay of thyroxine, 3,5,3'-triiodothyronine, progesterone, testosterone, estradiol, cortisol, and insulin in equine, bovine, and canine plasma. In the first experiment, hormones were measured in plasma obtained from hemolyzed blood that had been stored for 18 hours. Blood samples were drawn from pregnant cows, male and diestrous female dogs, and male and pregnant female horses. Each sample was divided into 2 equal portions. One portion was ejected 4 times with a syringe through a 20-gauge (dogs, horses) or 22-gauge (cows) hypodermic needle to induce variable degrees of hemolysis. Two subsamples of the blood were taken before the first and after the first, second, and fourth ejections. One subsample of each pair was stored at 2 to 4 C and the other was stored at 20 to 22 C for 18 to 22 hours before plasma was recovered and stored at -20 C. The second portion of blood from each animal was centrifuged after collection; plasma was recovered and treated similarly as was blood. Concentrations of thyroxine in equine plasma, of 3,5,3'-triiodothyronine, estradiol, and testosterone in equine and canine plasma, and of cortisol in equine plasma were not affected by hemolysis.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1991-07-01 PubMed ID: 1892262
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates how hemolysis, the breakdown of red blood cells, and storage conditions affect the quantification of seven different hormones in equine, bovine, and canine plasma. They found that hemolysis did not affect certain hormone concentrations in equine and canine plasma.
Experimental Process
- The study involved measuring hormone levels in plasma obtained from hemolyzed blood that had been stored for 18 hours.
- Blood samples were taken from pregnant cows, male and diestrous female dogs, and male and pregnant horses and each sample was divided into two equal parts.
- One part was subjected to hemolysis by being ejected four times through a hypodermic needle inducing variable degrees of hemolysis.
- Subsamples of the blood were taken before and after the first, second, and fourth ejections.
- From each pair, one subsample was stored at 2 to 4 degrees Celsius and the other at 20 to 22 degrees Celsius for 18 to 22 hours before plasma was separated and stored at -20 degrees Celsius.
- The second part of blood from each animal was centrifuged immediately after collection, the plasma separated and treated in the same manner as the subsamples.
Results
- Concentrations of thyroxine in equine plasma, of 3,5,3′-triiodothyronine, estradiol, and testosterone in equine and canine plasma, and of cortisol in equine plasma, were not affected by the process of hemolysis.
- The results suggest that the process of hemolysis does not interfere with the quantification of these hormones and therefore may not necessarily impact diagnostic endocrinology laboratory analysis for these animals.
In conclusion, the results provide valuable guidelines for veterinary diagnostic endocrinology laboratories regarding the effects of hemolysis and sample storage on hormone quantification. It also provides an important basis for further research and analysis on the topic.
Cite This Article
APA
Reimers TJ, Lamb SV, Bartlett SA, Matamoros RA, Cowan RG, Engle JS.
(1991).
Effects of hemolysis and storage on quantification of hormones in blood samples from dogs, cattle, and horses.
Am J Vet Res, 52(7), 1075-1080.
Publication
Researcher Affiliations
- Diagnostic Laboratory, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
MeSH Terms
- Animals
- Blood Preservation
- Cattle / blood
- Centrifugation
- Diestrus / blood
- Dogs / blood
- Female
- Hemolysis
- Hormones / blood
- Horses / blood
- Male
- Pregnancy
- Radioimmunoassay
- Reference Values
- Temperature
- Thyroid Hormones / blood
Citations
This article has been cited 11 times.- Koutsoumanis K, Allende A, Alvarez-Ordóñez A, Bolton D, Bover-Cid S, Chemaly M, Davies R, De Cesare A, Herman L, Lindqvist R, Nauta M, Peixe L, Ru G, Simmons M, Skandamis P, Suffredini E, Sánchez JÁ, Blagojevic B, Fürst P, Garin-Bastuji B, Jensen HE, Paulsen P, Baert K, Barrucci F, Broglia A, Georgiadis M, Hempen M, Hilbert F. Evaluation of public and animal health risks in case of a delayed post-mortem inspection in ungulates. EFSA J 2020 Dec;18(12):e06307.
- Nöthling JO, De Cramer KGM. Comparison of progesterone assay by chemiluminescence or radioimmunoassay for clinical decision-making in canine reproduction. J S Afr Vet Assoc 2019 Oct 22;90(0):e1-e6.
- Bertin FR, Taylor SD, Bianco AW, Sojka-Kritchevsky JE. The Effect of Fasting Duration on Baseline Blood Glucose Concentration, Blood Insulin Concentration, Glucose/Insulin Ratio, Oral Sugar Test, and Insulin Response Test Results in Horses. J Vet Intern Med 2016 Sep;30(5):1726-1731.
- Whitlock BK, Daniel JA, Amelse LL, Tanco VM, Chameroy KA, Schrick FN. Kisspeptin receptor agonist (FTM080) increased plasma concentrations of luteinizing hormone in anestrous ewes. PeerJ 2015;3:e1382.
- Randolph JF, Lamb SV, Cheraskin JL, Schanbacher BJ, Salerno VJ, Mack KM, Scarlett JM, Place NJ. Free thyroxine concentrations by equilibrium dialysis and chemiluminescent immunoassays in 13 hypothyroid dogs positive for thyroglobulin antibody. J Vet Intern Med 2015 May-Jun;29(3):877-81.
- de Mestre AM, Hanlon D, Adams AP, Runcan E, Leadbeater JC, Erb HN, Costa CC, Miller D, Allen WR, Antczak DF. Functions of ectopically transplanted invasive horse trophoblast. Reproduction 2011 Jun;141(6):849-56.
- Bakkum BW, Fan L, Pandey SC, Cohen RS. Hetereogeneity of dose and time effects of estrogen on neuron-specific neuronal protein and phosphorylated cyclic AMP response element-binding protein in the hippocampus of ovariectomized rats. J Neurosci Res 2011 Jun;89(6):883-97.
- Fan L, Pandey SC, Cohen RS. Estrogen affects levels of Bcl-2 protein and mRNA in medial amygdala of ovariectomized rats. J Neurosci Res 2008 Dec;86(16):3655-64.
- Kim NN, Stankovic M, Cushman TT, Goldstein I, Munarriz R, Traish AM. Streptozotocin-induced diabetes in the rat is associated with changes in vaginal hemodynamics, morphology and biochemical markers. BMC Physiol 2006 May 30;6:4.
- Batista MR, Smith MS, Snead WL, Connolly CC, Lacy DB, Moore MC. Chronic estradiol and progesterone treatment in conscious dogs: effects on insulin sensitivity and response to hypoglycemia. Am J Physiol Regul Integr Comp Physiol 2005 Oct;289(4):R1064-73.
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