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Home / Equine Research Bank / Food Sci Anim Resour / Effects of Horse Meat Hydrolysate on Oxidative Stress, Proin...
Food science of animal resources2024; 44(1); 132-145; doi: 10.5851/kosfa.2023.e65

Effects of Horse Meat Hydrolysate on Oxidative Stress, Proinflammatory Cytokines, and the Ubiquitin-Proteasomal System of C2C12 Cells.

Abstract: Sarcopenia, the age-related muscle atrophy, is a serious concern as it is associated with frailty, reduced physical functions, and increased mortality risk. Protein supplementation is essential for preserving muscle mass, and horse meat can be an excellent source of proteins. Since sarcopenia occurs under conditions of oxidative stress, this study aimed to investigate the potential anti-muscle atrophy effect of horse meat hydrolysate using C2C12 cells. A horse meat hydrolysate less than 3 kDa (A4<3kDa) significantly increased the viability of C2C12 myoblasts against HO-induced cytotoxicity. Exposure of C2C12 myoblasts to lipopolysaccharide led to an elevation of cellular reactive oxygen species levels and mRNA expression of proinflammatory cytokines, including tumor necrosis factor-α and interleukin 6, and these effects were attenuated by A4<3kDa treatment. Additionally, A4<3kDa activated protein synthesis-related proteins through the protein kinase B/mechanistic target of rapamycin pathway, while decreasing the expression of activity and degradation-related proteins, such as Forkhead box O3, muscle RING finger protein-1, and Atrogin-1 in dexamethasone-treated C2C12 myotubes. Therefore, the natural material A4<3kDa has the potential ofprotecting against muscle atrophy, while further study is needed.
© Korean Society for Food Science of Animal Resources.
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Publication Date: 2024-01-01 PubMed ID: 38229864PubMed Central: PMC10789556DOI: 10.5851/kosfa.2023.e65Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigates the potential benefits of horse meat hydrolysate on combating muscle atrophy by examining its effects in stress-induced C2C12 muscle cells.

Introduction and Objective

  • The study primarily focusses on sarcopenia, a condition characterised by loss of muscle mass and function with age. The disorder is linked with increased frailTy, decreased physical abilities, and heightened risk of mortality.
  • The researchers highlight the importance of protein supplementation in maintaining muscle mass. They suggest that horse meat could be a valuable protein source, particularly beneficial in the management of sarcopenia, which occurs in conditions associated with oxidative stress.
  • To examine the anti-atrophy effects of horse meat, the researchers used a hydrolysate of horse meat less than 3 kDa (A4<3kDa), and examined its effects on C2C12 muscle cells.

Findings and Observations

  • The application of A4<3kDa significantly enhanced the viability of C2C12 myoblasts against oxidative stress induced by the addition of hydrogen peroxide (H2O2).
  • When the myoblasts were exposed to lipopolysaccharide, they exhibited higher cellular levels of reactive oxygen species and exhibited increased mRNA expression of proinflammatory cytokines, such as tumor necrosis factor-alpha and interleukin 6. These effects, however, were reduced by treating the cells with A4<3kDa.
  • Furthermore, A4<3kDa was observed to activate proteins associated with protein synthesis through the protein kinase B/mechanistic target of rapamycin pathway, while it decreased the expression of proteins involved in degradation and activity, including Forkhead box O3, muscle RING finger protein-1, and Atrogin-1 in dexamethasone-treated C2C12 myotubes.

Conclusion

  • These observations indicated that the horse meat hydrolysate A4<3kDa could potentially safeguard against muscle atrophy. The authors, however, emphasise that further investigation is needed to fully understand the implications and applicability of these results in the clinical setting.

Cite This Article

APA
Lee HJ, Kim D, Do K, Yang CB, Jeon SW, Jang A. (2024). Effects of Horse Meat Hydrolysate on Oxidative Stress, Proinflammatory Cytokines, and the Ubiquitin-Proteasomal System of C2C12 Cells. Food Sci Anim Resour, 44(1), 132-145. https://doi.org/10.5851/kosfa.2023.e65

Publication

Food science of animal resources
ISSN: 2636-0780
NlmUniqueID: 101742126
Country: Korea (South)
Language: English
Volume: 44
Issue: 1
Pages: 132-145

Researcher Affiliations

Lee, Hee-Jeong
  • Department of Applied Animal Science, Kangwon National University, Chuncheon 24341, Korea.
Kim, Dongwook
  • Department of Applied Animal Science, Kangwon National University, Chuncheon 24341, Korea.
Do, Kyoungtag
  • Department of Animal Biotechnology, Jeju National University, Jeju 63243, Korea.
Yang, Chang-Beom
  • Department of Animal Biotechnology, Jeju National University, Jeju 63243, Korea.
Jeon, Seong-Won
  • Department of Animal Biotechnology, Jeju National University, Jeju 63243, Korea.
Jang, Aera
  • Department of Applied Animal Science, Kangwon National University, Chuncheon 24341, Korea.

Conflict of Interest Statement

The authors declare no potential conflicts of interest.

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