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Effects of intravenous lipopolysaccharide infusion on glucose and insulin dynamics in horses with equine metabolic syndrome.
Abstract: To test the hypothesis that glucose and insulin dynamics during endotoxemia differ between healthy horses and horses with equine metabolic syndrome (EMS). Methods: 6 healthy adult mares and 6 horses with EMS. Methods: Each horse randomly received an IV infusion of lipopolysaccharide (20 ng/kg [in 60 mL of sterile saline {0.9% NaCl} solution]) or saline solution, followed by the other treatment after a 7-day washout period. Baseline insulin-modified frequently sampled IV glucose tolerance tests were performed 27 hours before and then repeated at 0.5 and 21 hours after infusion. Results were assessed via minimal model analysis and area under the curve values for plasma glucose and serum insulin concentrations. Results: Lipopolysaccharide infusion decreased insulin sensitivity and increased area under the serum insulin concentration curve (treatment × time) in both healthy and EMS-affected horses, compared with findings following saline solution administration. The magnitude of increase in area under the plasma glucose curve following LPS administration was greater for the EMS-affected horses than it was for the healthy horses. Horses with EMS that received LPS or saline solution infusions had decreased insulin sensitivity over time. Conclusions: Glucose and insulin responses to endotoxemia differed between healthy horses and horses with EMS, with greater loss of glycemic control in EMS-affected horses. Horses with EMS also had greater derangements in glucose and insulin homeostasis that were potentially stress induced. It may therefore be helpful to avoid exposure of these horses to stressful situations.
Publication Date: 2013-06-28 PubMed ID: 23802674DOI: 10.2460/ajvr.74.7.1020Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Randomized Controlled Trial
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research investigates the impact of an invasive bacterial substance infusion on insulin and glucose regulation in healthy horses and those suffering from a condition known as equine metabolic syndrome (EMS). The study found that horses with EMS had greater disruptions in glucose regulation and insulin level regulation than healthy horses, particularly under stressful conditions.
Methodology
- The study involved 12 horses, half of which were healthy and the other half diagnosed with equine metabolic syndrome (EMS).
- Each horse received an intravenous (IV) infusion of lipopolysaccharide, which is a bacterial endotoxin, or a saline solution instead. Each treatment was followed by a 7-day washout period before the other treatment was administered. The lipopolysaccharide was administered at a dosage of 20 ng/kg and it was diluted in a 60 mL saline solution.
- The insulin-modified frequently sampled intravenous glucose tolerance test was performed 27 hours before and 0.5 and 21 hours after the infusion.
- The researchers analyzed the results taking into consideration insulin and glucose levels in the body using the area under the curve values for plasma glucose and serum insulin concentrations.
Results
- The infusion of lipopolysaccharide decreased insulin sensitivity in both healthy and EMS-diagnosed horses compared to the saline solution.
- The glucose area under the curve magnified more for the EMS-diagnosed horses compared to healthy horses, following lipopolysaccharide injection.
- Horses with EMS showed a consistent decrease in insulin sensitivity over time, regardless of whether they received the lipopolysaccharide or saline solution administrations.
Conclusions
- The study concluded that healthy horses and those with EMS responded differently to endotoxemia, with EMS-diagnosed horses experiencing greater disruptions to their insulin and glucose regulation.
- The findings of the research also suggested that horses with EMS had greater disruptions in glucose regulation and insulin level regulation possibly due to external stressors. Based on this outcome, it’s recommended that these horses be kept away from stressful conditions as far as possible.
Cite This Article
APA
Tadros EM, Frank N, De Witte FG, Boston RC.
(2013).
Effects of intravenous lipopolysaccharide infusion on glucose and insulin dynamics in horses with equine metabolic syndrome.
Am J Vet Res, 74(7), 1020-1029.
https://doi.org/10.2460/ajvr.74.7.1020 Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USA.
MeSH Terms
- Animals
- Blood Glucose / drug effects
- Blood Glucose / metabolism
- Female
- Horse Diseases / blood
- Horse Diseases / metabolism
- Horses
- Injections, Intravenous
- Insulin / metabolism
- Lipopolysaccharides / administration & dosage
- Lipopolysaccharides / toxicity
- Male
Citations
This article has been cited 3 times.- Sp N, Kang DY, Kim DH, Lee HG, Park YM, Kim IH, Lee HK, Cho BW, Jang KJ, Yang YM. Methylsulfonylmethane inhibits cortisol-induced stress through p53-mediated SDHA/HPRT1 expression in racehorse skeletal muscle cells: A primary step against exercise stress. Exp Ther Med 2020 Jan;19(1):214-222.
- Bertin FR, Ruffin-Taylor D, Stewart AJ. Insulin dysregulation in horses with systemic inflammatory response syndrome. J Vet Intern Med 2018 Jul;32(4):1420-1427.
- Krueger CR, Ruple-Czerniak A, Hackett ES. Evaluation of plasma muscle enzyme activity as an indicator of lesion characteristics and prognosis in horses undergoing celiotomy for acute gastrointestinal pain. BMC Vet Res 2014;10 Suppl 1(Suppl 1):S7.
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