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Reproductive biology and endocrinology : RB&E2009; 7; 113; doi: 10.1186/1477-7827-7-113

Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse.

Abstract: The identification of the adipocyte-derived obesity gene product, leptin (Ob), and subsequently its association with reproduction in rodents and humans led to speculations that leptin may be involved in the regulation of oocyte and preimplantation embryo development. In mice and pigs, in vitro leptin addition significantly increased meiotic resumption and promoted preimplantation embryo development in a dose-dependent manner. This study was conducted to determine whether leptin supplementation during in vitro maturation (IVM) to horse oocytes could have effects on their developmental capacity after fertilization by IntraCytoplasmic Sperm Injection (ICSI). Methods: Compact and expanded-cumulus horse oocytes were matured in medium containing different concentrations (1, 10, 100, 1000 ng/ml) of recombinant human leptin and the effects on maturation, fertilization and embryo cleavage were evaluated. Furthermore, early developmental expression of Ob and leptin receptor (Ob-R) was investigated by immunocytochemical staining. Results: In expanded-cumulus oocytes, the addition of leptin in IVM medium improved maturation (74% vs 44%, for 100 ng/ml leptin-treated and control groups, respectively; P < 0.05) and fertilization after ICSI (56% vs 23% for 10 ng/ml leptin-treated and control groups, respectively; P < 0.05). However, the developmental rate and quality of 8-cell stage embryos derived from leptin-treated oocytes (100 ng/ml) was significantly reduced, in contrast to previous data in other species where leptin increased embryo cleavage. Ob and Ob-R proteins were detected up to the 8-cell stage with cortical and cytoplasmic granule-like distribution pattern in each blastomere. Conclusions: Leptin plays a cumulus cell-mediated role in the regulation of oocyte maturation in the mare. Species-specific differences may exist in oocyte sensitivity to leptin.
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Publication Date: 2009-10-16 PubMed ID: 19835605PubMed Central: PMC2774312DOI: 10.1186/1477-7827-7-113Google Scholar: Lookup
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  • Comparative Study
  • Evaluation Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates how the hormone leptin, which is typically associated with obesity, affects the development of horse oocytes after being fertilized using Intracytoplasmic Sperm Injection (ICSI). The researchers found that adding leptin during the In Vitro Maturation (IVM) stage increases the maturation and fertilization rates of horse oocytes, but reduces the development rate and quality of the resultant 8-cell stage embryos.

Objective and Methodology

  • The study was aimed at understanding the role of leptin supplementation during the in vitro maturation (IVM) of horse oocytes and its impact on their developmental capacity after being fertilized by Intracytoplasmic Sperm Injection (ICSI).
  • The researchers used horse oocytes with both compact and expanded-cumulus and exposed them to different concentrations of recombinant human leptin during IVM.

Results on maturation and fertilization

  • It was found that for expanded-cumulus oocytes, the addition of leptin significantly improved maturation and fertilization rates after ICSI.
  • A leptin concentration of 100 ng/ml in the IVM medium notably improved maturation, compared to the control groups.
  • Furthermore, a leptin concentration of 10 ng/ml significantly improved fertilization rates post-ICSI.

Results on Embryo Cleavage and Quality

  • However, the study found that the development rate and quality of 8-cell stage embryos derived from leptin-treated oocytes (at a concentration of 100 ng/ml) were significantly reduced.
  • This finding contrasted with data from other species where leptin was found to increase embryo cleavage. Such a result indicates that there might be species-specific differences in the sensitivity of oocytes to leptin.

Leptin and Leptin Receptor Protein Expression

  • This study also investigated the expression of leptin (Ob) and leptin receptor (Ob-R) proteins during early stages of development.
  • Both proteins were detected up to the 8-cell stage and demonstrated a granule-like distribution pattern in the cytoplasm and cortex of each blastomere.

Conclusions

  • Based on the results, the researchers concluded that leptin plays a role in the regulation of oocyte maturation in horses, mediated by cumulus cells.
  • However, the variations in the outcomes between studies on different species suggest that there may exist species-specific differences in oocyte sensitivity to leptin.

Cite This Article

APA
Lange Consiglio A, Dell'Aquila ME, Fiandanese N, Ambruosi B, Cho YS, Bosi G, Arrighi S, Lacalandra GM, Cremonesi F. (2009). Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse. Reprod Biol Endocrinol, 7, 113. https://doi.org/10.1186/1477-7827-7-113

Publication

Reproductive biology and endocrinology : RB&E
ISSN: 1477-7827
NlmUniqueID: 101153627
Country: England
Language: English
Volume: 7
Pages: 113

Researcher Affiliations

Lange Consiglio, Anna
  • Università degli Studi di Milano, Reproduction Unit, Large Animal Hospital, Faculty of Veterinary Medicine, Via dell'Università 6,-26900 Lodi, Italy. anna.langeconsiglio@unimi.it
Dell'Aquila, Maria Elena
    Fiandanese, Nadia
      Ambruosi, Barbara
        Cho, Yoon S
          Bosi, Giampaolo
            Arrighi, Silvana
              Lacalandra, Giovanni M
                Cremonesi, Fausto

                  MeSH Terms

                  • Animals
                  • Cells, Cultured
                  • Cleavage Stage, Ovum / cytology
                  • Cleavage Stage, Ovum / drug effects
                  • Embryo, Mammalian
                  • Embryonic Development / drug effects
                  • Female
                  • Fertilization / drug effects
                  • Horses / metabolism
                  • Horses / physiology
                  • Leptin / metabolism
                  • Leptin / pharmacology
                  • Male
                  • Oogenesis / drug effects
                  • Quality Control
                  • Receptors, Leptin / metabolism
                  • Sperm Injections, Intracytoplasmic

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