Effects of platelet-derived growth factor-BB on the metabolic function and morphologic features of equine tendon in explant culture.
Abstract: To evaluate the effects of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) on the metabolic function and morphologic features of equine superficial digital flexor tendon (SDFT) in explant culture. Animals-6 euthanized horses (2 to 5 years old). Methods: Forelimb SDFT explants were cultured for 6 days as untreated control specimens or treated with rhPDGF-BB (1, 10, 50, or 100 ng/mL of medium). Treatment effects on explant gene expression were evaluated via real-time PCR analysis of collagen type I, collagen type III, PDGF-A, and PDGF-B mRNA. Explants were assayed for total collagen, glycosaminoglycan, and DNA content; histologic changes were assessed via H and E staining and immunohistochemical localization of collagen types I and III. Results: No morphologic or proliferative changes were detected in tendon explant sections. After high-dose rhPDGF-BB treatment, gene expression of collagen types I and III was increased and decreased, respectively. Expression of PDGF-A and PDGF-B mRNA was significantly increased at 24 hours, but later decreased to have few or negative autoinductive effects. Although PDGF gene expression waned after 48 hours of culture, collagen type I gene expression was significantly increased at 48 hours and reached peak value on day 6. Glycosaminoglycan and DNA content of explants were unchanged with rhPDGF-BB treatment. Conclusions: Results suggest that rhPDGF-BB use may be of benefit in the repair of equine tendon, particularly through induction of collagen type I mRNA. Positive autoinductive effects of PDGF-BB in equine SDFT explants were detected early following culture medium supplementation, but these diminished with time.
Publication Date: 2006-09-05 PubMed ID: 16948608DOI: 10.2460/ajvr.67.9.1595Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research focuses on the effects of human platelet-derived growth factor-BB (rhPDGF-BB) on the metabolic performance and shape characteristics of horse tendons, in particular the superficial digital flexor tendon. The study suggests that rhPDGF-BB could help in the healing process of horse tendons through the induction of a particular type of collagen.
Research Methods
- The tendon samples for the study were extracted from the forelimb superficial digital flexor tendon (SDFT) of six euthanized horses, aged between 2 to 5 years old.
- The specimens were cultured for six days, with some left untreated while others were treated with varying concentrations of recombinant human platelet-derived growth factor-BB (rhPDGF-BB).
- The researchers evaluated the effect of rhPDGF-BB treatment by using real-time PCR (Polymerase Chain Reaction) analysis to assess the expression of various genes, including collagen type I, collagen type III, PDGF-A, and PDGF-B.
- The total collagen, glycosaminoglycan, and DNA content in the specimens were measured, and any histological changes were evaluated through H&E staining and immunohistochemical identification of collagen types I and III.
Research Findings
- No morphological or proliferative changes were observed in the tendon samples.
- High-dose rhPDGF-BB treatment led to an increase in the gene expression of collagen type I and a decrease in collagen type III.
- PDGF-A and PDGF-B mRNA expression showed significant growth at the 24-hour mark, but later dropped off, suggesting few or negative autoinductive effects.
- Despite the decline in PDGF gene expression after 48 hours of culture, the expression of collagen type I gene significantly increased at the 48-hour mark and reached its peak value on day 6.
- The glycosaminoglycan and DNA contents of the samples remained unchanged with the rhPDGF-BB treatment.
Conclusions
- The findings suggest that the use of rhPDGF-BB could be beneficial in the repair of horse tendons, especially due to its induction of collagen type I mRNA.
- Positive autoinductive effects of PDGF-BB were observed in the early stages of culture medium supplementation, but these effects diminished over time.
Cite This Article
APA
Haupt JL, Donnelly BP, Nixon AJ.
(2006).
Effects of platelet-derived growth factor-BB on the metabolic function and morphologic features of equine tendon in explant culture.
Am J Vet Res, 67(9), 1595-1600.
https://doi.org/10.2460/ajvr.67.9.1595 Publication
Researcher Affiliations
- Comparative Orthopaedics Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
MeSH Terms
- Animals
- Becaplermin
- Collagen Type I / genetics
- Collagen Type I / metabolism
- Collagen Type III / genetics
- Collagen Type III / metabolism
- DNA / genetics
- Dose-Response Relationship, Drug
- Gene Expression / drug effects
- Glycosaminoglycans / metabolism
- Horses / metabolism
- Immunohistochemistry / veterinary
- Platelet-Derived Growth Factor / genetics
- Platelet-Derived Growth Factor / metabolism
- Platelet-Derived Growth Factor / pharmacology
- Proto-Oncogene Proteins c-sis
- RNA, Messenger / biosynthesis
- RNA, Messenger / genetics
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- Tendons / drug effects
- Tendons / metabolism
- Tissue Culture Techniques
Citations
This article has been cited 7 times.- Chen Y, Jiang L, Lyu K, Lu J, Long L, Wang X, Liu T, Li S. A Promising Candidate in Tendon Healing Events-PDGF-BB. Biomolecules 2022 Oct 20;12(10).
- Roberts JH, Halper J. Growth Factor Roles in Soft Tissue Physiology and Pathophysiology. Adv Exp Med Biol 2021;1348:139-159.
- Lange-Consiglio A, Cazzaniga N, Garlappi R, Spelta C, Pollera C, Perrini C, Cremonesi F. Platelet concentrate in bovine reproduction: effects on in vitro embryo production and after intrauterine administration in repeat breeder cows. Reprod Biol Endocrinol 2015 Jun 19;13:65.
- Narang I, Mittal N, Mishra N. A comparative evaluation of the blood clot, platelet-rich plasma, and platelet-rich fibrin in regeneration of necrotic immature permanent teeth: A clinical study. Contemp Clin Dent 2015 Jan-Mar;6(1):63-8.
- Mishra N, Narang I, Mittal N. Platelet-rich fibrin-mediated revitalization of immature necrotic tooth. Contemp Clin Dent 2013 Jul;4(3):412-5.
- Murray SJ, Santangelo KS, Bertone AL. Evaluation of early cellular influences of bone morphogenetic proteins 12 and 2 on equine superficial digital flexor tenocytes and bone marrow-derived mesenchymal stem cells in vitro. Am J Vet Res 2010 Jan;71(1):103-14.
- Huang R, Chen W, Fang M, Peters OA, Hosseinpour S. Clinical and Radiographic Outcomes of Autologous Platelet-Rich Products in Regenerative Endodontics: A Systematic Review and Meta-Analysis. Dent J (Basel) 2025 May 26;13(6).
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