FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Vet J / Effects of pro-inflammatory cytokines on chondrogenesis of e...
Veterinary journal (London, England : 1997)2016; 217; 26-32; doi: 10.1016/j.tvjl.2016.05.014

Effects of pro-inflammatory cytokines on chondrogenesis of equine mesenchymal stromal cells derived from bone marrow or synovial fluid.

Abstract: Mesenchymal stromal cells (MSCs) have the capacity to differentiate into cells of mesenchymal lineage, such as chondrocytes, and have potential for use in regeneration of equine articular cartilage. MSCs instilled intra-articularly would be exposed to the inflamed environment associated with equine osteoarthritis (OA), which may compromise their function and ability to heal a cartilaginous defect. The aim of this study was to assess the ability of equine adult MSCs to differentiate into chondrocytes when stimulated with pro-inflammatory cytokines. MSCs derived from equine bone marrow (BM) and from synovial fluid (SF) were cultured in chondrogenic induction medium containing transforming growth factor (TGF)-β1. BM-derived MSCs (BMMSCs) and SF-derived MSCs (SFMSCs) were stimulated with 100 ng/mL interferon (IFN)-γ and 10 ng/mL tumor necrosis factor (TNF)-α. Chondrogenic differentiation was measured quantitatively with the glycosaminoglycan (GAG) assay and qualitatively by immunofluorescence (IF) for SOX-9, TGF-β1, aggrecan and collagen II. The viability of equine MSCs was maintained in the presence of IFN-γ and TNF-α, but production of GAGs from both types of MSCs was decreased in stimulated medium. Exposure of BMMSCs to pro-inflammatory cytokines reduced the levels of SOX-9, TGF-β1, aggrecan and collagen II, whereas exposure of SFMSCs to these cytokines reduced the levels of aggrecan only. These data suggest that pro-inflammatory cytokines do not affect proliferation of MSCs, but could inhibit chondrogenesis of MSCs.
Published by Elsevier Ltd.
Get Full Text
Publication Date: 2016-05-31 PubMed ID: 27810206DOI: 10.1016/j.tvjl.2016.05.014Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the impact of pro-inflammatory cytokines on the ability of specific horse stem cells to develop into chondrocytes. The findings show that these cytokines do not affect stem cell proliferation, but could limit their development into chondrocytes.

Objective of the Study

  • The study aims to evaluate how pro-inflammatory cytokines affect the chondrogenesis (the process of cartilage formation) of mesenchymal stromal cells (MSCs) derived from horse bone marrow and synovial fluid. This has significance given the potential of MSCs to be used in repairing cartilage defects, particularly in the context of osteoarthritis, which is common in equines.

Methodology

  • MSCs acquired from equine bone marrow and synovial fluid were cultured in a growth medium with transforming growth factor (TGF)-β1, a protein that stimulates cell growth and differentiation.
  • Both types of MSCs (from bone marrow, BMMSCs, and from synovial fluid, SFMSCs) were exposed to interferon (IFN)-γ and tumor necrosis factor (TNF)-α, both of which are pro-inflammatory cytokines, substances secreted by certain cells of the immune system that have an effect on other cells.
  • The researchers then measured chondrogenic differentiation quantitively using the glycosaminoglycan (GAG) assay and qualitatively through immunofluorescence for SOX-9, TGF-β1, aggrecan and collagen II, markers indicative of the chondrogenesis process.

Findings

  • The study found that the viability of MSCs remained intact despite the presence of IFN-γ and TNF-α, indicating that these pro-inflammatory cytokines do not hinder cell proliferation.
  • However, the production of GAGs, compounds that form part of the ground substance of cartilage, decreased for both types of MSCs in the cytokine-treated medium, signifying a potential hindrance to chondrogenesis.
  • BMMSCs exposed to these cytokines also showed reduced levels of SOX-9, TGF-β1, aggrecan and collagen II, indicating a negative impact on chondrocyte differentiation. Meanwhile, SFMSCs showed only reduced levels of aggrecan when exposed to the cytokines.

In conclusion, the study suggests that while pro-inflammatory cytokines do not appear to affect the proliferation of MSCs, they could potentially negatively influence their ability to differentiate into chondrocytes. This is a significant finding for the potential use of MSCs in regenerative therapies for osteoarthritis.

Cite This Article

APA
Zayed MN, Schumacher J, Misk N, Dhar MS. (2016). Effects of pro-inflammatory cytokines on chondrogenesis of equine mesenchymal stromal cells derived from bone marrow or synovial fluid. Vet J, 217, 26-32. https://doi.org/10.1016/j.tvjl.2016.05.014

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 217
Pages: 26-32
PII: S1090-0233(16)30068-5

Researcher Affiliations

Zayed, M N
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive c247, Knoxville, TN 37996, USA; Department of Animal Surgery, College of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
Schumacher, J
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive c247, Knoxville, TN 37996, USA.
Misk, N
  • Department of Animal Surgery, College of Veterinary Medicine, Assuit University, Assuit 71526, Egypt.
Dhar, M S
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive c247, Knoxville, TN 37996, USA. Electronic address: mdhar@utk.edu.

MeSH Terms

  • Animals
  • Bone Marrow / physiology
  • Cell Differentiation
  • Chondrocytes / physiology
  • Chondrogenesis
  • Cytokines / metabolism
  • Horses / physiology
  • Mesenchymal Stem Cells / cytology
  • Synovial Fluid / physiology

Citations

This article has been cited 17 times.
  1. Genç D, Sezer Kürkçü M, Yiğittürk G, Günaydın B, Elbe H, Aladağ A, Çöl B, Tarhan EF. Synovial fluid niche promoted differentiation of dental follicle mesenchymal stem cells toward chondrogenesis in rheumatoid arthritis.. Arch Rheumatol 2022 Mar;37(1):94-109.
    doi: 10.46497/ArchRheumatol.2022.8891pubmed: 35949879google scholar: lookup
  2. Bhogoju S, Khan S, Subramanian A. Continuous Low-Intensity Ultrasound Preserves Chondrogenesis of Mesenchymal Stromal Cells in the Presence of Cytokines by Inhibiting NFκB Activation.. Biomolecules 2022 Mar 11;12(3).
    doi: 10.3390/biom12030434pubmed: 35327626google scholar: lookup
  3. Yue D, Du L, Zhang B, Wu H, Yang Q, Wang M, Pan J. Time-dependently Appeared Microenvironmental Changes and Mechanism after Cartilage or Joint Damage and the Influences on Cartilage Regeneration.. Organogenesis 2021 Oct 2;17(3-4):85-99.
    doi: 10.1080/15476278.2021.1991199pubmed: 34806543google scholar: lookup
  4. Bohaud C, Contreras-Lopez R, De La Cruz J, Terraza-Aguirre C, Wei M, Djouad F, Jorgensen C. Pro-regenerative Dialogue Between Macrophages and Mesenchymal Stem/Stromal Cells in Osteoarthritis.. Front Cell Dev Biol 2021;9:718938.
    doi: 10.3389/fcell.2021.718938pubmed: 34604219google scholar: lookup
  5. Fülber J, Agreste FR, Seidel SRT, Sotelo EDP, Barbosa ÂP, Michelacci YM, Baccarin RYA. Chondrogenic potential of mesenchymal stem cells from horses using a magnetic 3D cell culture system.. World J Stem Cells 2021 Jun 26;13(6):645-658.
    doi: 10.4252/wjsc.v13.i6.645pubmed: 34249233google scholar: lookup
  6. Zayed M, Adair S, Dhar M. Effects of Normal Synovial Fluid and Interferon Gamma on Chondrogenic Capability and Immunomodulatory Potential Respectively on Equine Mesenchymal Stem Cells.. Int J Mol Sci 2021 Jun 15;22(12).
    doi: 10.3390/ijms22126391pubmed: 34203758google scholar: lookup
  7. Bartolotti I, Roseti L, Petretta M, Grigolo B, Desando G. A Roadmap of In Vitro Models in Osteoarthritis: A Focus on Their Biological Relevance in Regenerative Medicine.. J Clin Med 2021 Apr 28;10(9).
    doi: 10.3390/jcm10091920pubmed: 33925222google scholar: lookup
  8. Ganiev I, Alexandrova N, Aimaletdinov A, Rutland C, Malanyeva A, Rizvanov A, Zakirova E. The treatment of articular cartilage injuries with mesenchymal stem cells in different animal species.. Open Vet J 2021 Jan-Mar;11(1):128-134.
    doi: 10.4314/ovj.v11i1.19pubmed: 33898294google scholar: lookup
  9. Neybecker P, Henrionnet C, Pape E, Grossin L, Mainard D, Galois L, Loeuille D, Gillet P, Pinzano A. Respective stemness and chondrogenic potential of mesenchymal stem cells isolated from human bone marrow, synovial membrane, and synovial fluid.. Stem Cell Res Ther 2020 Jul 25;11(1):316.
    doi: 10.1186/s13287-020-01786-5pubmed: 32711576google scholar: lookup
  10. Zhu H, Hu Y, Wang C, Zhang X, He D. CircGCN1L1 promotes synoviocyte proliferation and chondrocyte apoptosis by targeting miR-330-3p and TNF-α in TMJ osteoarthritis.. Cell Death Dis 2020 Apr 24;11(4):284.
    doi: 10.1038/s41419-020-2447-7pubmed: 32332704google scholar: lookup
  11. Lo Monaco M, Gervois P, Beaumont J, Clegg P, Bronckaers A, Vandeweerd JM, Lambrichts I. Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis.. Cells 2020 Apr 15;9(4).
    doi: 10.3390/cells9040980pubmed: 32326610google scholar: lookup
  12. Gugjoo MB, Hussain S, Amarpal, Shah RA, Dhama K. Mesenchymal Stem Cell-Mediated Immuno-Modulatory and Anti- Inflammatory Mechanisms in Immune and Allergic Disorders.. Recent Pat Inflamm Allergy Drug Discov 2020;14(1):3-14.
    doi: 10.2174/1872213X14666200130100236pubmed: 32000656google scholar: lookup
  13. van der Kraan PM. The Interaction between Joint Inflammation and Cartilage Repair.. Tissue Eng Regen Med 2019 Aug;16(4):327-334.
    doi: 10.1007/s13770-019-00204-zpubmed: 31413937google scholar: lookup
  14. Gugjoo MB, Fazili MR, Gayas MA, Ahmad RA, Dhama K. Animal mesenchymal stem cell research in cartilage regenerative medicine - a review.. Vet Q 2019 Dec;39(1):95-120.
    doi: 10.1080/01652176.2019.1643051pubmed: 31291836google scholar: lookup
  15. Liu X, Niu Y, Xie W, Wei D, Du Q. Tanshinone IIA promotes osteogenic differentiation of human periodontal ligament stem cells via ERK1/2-dependent Runx2 induction.. Am J Transl Res 2019;11(1):340-350.
    pubmed: 30787991
  16. Neybecker P, Henrionnet C, Pape E, Mainard D, Galois L, Loeuille D, Gillet P, Pinzano A. In vitro and in vivo potentialities for cartilage repair from human advanced knee osteoarthritis synovial fluid-derived mesenchymal stem cells.. Stem Cell Res Ther 2018 Nov 28;9(1):329.
    doi: 10.1186/s13287-018-1071-2pubmed: 30486903google scholar: lookup
  17. Zayed M, Newby S, Misk N, Donnell R, Dhar M. Xenogenic Implantation of Equine Synovial Fluid-Derived Mesenchymal Stem Cells Leads to Articular Cartilage Regeneration.. Stem Cells Int 2018;2018:1073705.
    doi: 10.1155/2018/1073705pubmed: 29977305google scholar: lookup
Subscribe to our newsletter
Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.