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Journal of equine science2023; 34(3); 67-72; doi: 10.1294/jes.34.67

Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells.

Abstract: Resveratrol (RSV; trans-3,5,4'-trihydroxystilbene) strongly activates sirtuin 1, and it and its analogue V29 enhance the proliferation of mesenchymal stem/stromal cells (MSCs).Although culture medium containing 5-azacytydine and RSV inhibits senescence of adipose tissue-derived MSCs isolated from horses with metabolic syndrome, few studies have reported the effects of RSV on equine bone marrow-derived MSCs (eBMMSCs) isolated from horses without metabolic syndrome. The aim of this study was to investigate the effects of RSV and V29 on the cell cycle of eBMMSCs. Following treatment with 5 µM RSV or 10 µM V29, the cell proliferation capacity of eBMMSCs derived from seven horses was evaluated by EdU (5-ethynyl-2'-deoxyuridine) and Ki-67 antibody assays. Brightfield images of cells and immunofluorescent images of EdU, Ki-67, and DAPI staining were recorded by fluorescence microscopy, and the number of cells positive for each was quantified and compared by Friedman's test at P<0.05. The growth fraction of eBMMSCs was significantly increased by RSV and V29 as measured by the EdU assay (control 28.1% ± 13.8%, V29 31.8% ± 14.6%, RSV 32.0% ± 10.8%; mean ± SD; P<0.05) but not as measured by the Ki-67 antibody assay (control 27.0% ± 11.2%, V29 27.4% ± 10.8%, RSV 27.7% ± 6.8%). RSV and V29 promoted progression of the cell cycle of eBMMSCs into the S phase and may be useful for eBMMSC expansion.
©2023 The Japanese Society of Equine Science.
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Publication Date: 2023-09-08 PubMed ID: 37781569PubMed Central: PMC10534064DOI: 10.1294/jes.34.67Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the effects of Resveratrol (RSV) and its analogue, V29, on the cell cycle of equine bone marrow-derived mesenchymal stem/stromal cells (eBMMSCs). It found that RSV and V29 promoted the cell cycle progression into the S phase, increasing cell proliferation, potentially useful in expanding eBMMSCs.

Research Purpose and Approach

  • The purpose of this study was to investigate the effects of the compound Resveratrol (RSV) and its analogue, V29, on the cell cycle of horse mesenchymal stem/stromal cells (MSCs) obtained from bone marrow (eBMMSCs). These compounds have been found to enhance the proliferation of MSCs, but their effects on eBMMSCs from horses without metabolic syndrome were less understood.
  • This research aims to fill the knowledge gap about the potential use of these compounds in the expansion of stem cells for therapeutic applications.
  • The researchers treated eBMMSCs derived from seven horses with 5 µM RSV or 10 µM V29, and evaluated their ability to proliferate using two different laboratory techniques: the EdU (5-ethynyl-2′-deoxyuridine) and Ki-67 antibody assays.

Methods and Findings

  • The researchers examined and recorded the number of proliferating cells through brightfield microscopy and immunofluorescent images, using EdU, Ki-67, and DAPI staining techniques.
  • They found that both RSV and V29 compounds increased the growth fraction of eBMMSCs as measured by the EdU assay, but not as measured by the Ki-67 antibody assay.
  • RSV and V29 were found to promote the progression of eBMMSCs’ cell cycle into the S phase, which is a crucial phase for DNA replication in the cell division process.
  • These findings were statistically significant, indicating that these compounds could indeed enhance cell proliferation and could be useful for eBMMSC expansion in potential clinical settings.

Implication and Conclusion

  • The findings of this study suggest potential utility of RSV and V29 in therapeutic applications related to stem cell expansion.
  • This study offers insights into the use of these compounds in enhancing the proliferation of eBMMSCs, which could have significant implications in stem cell research and regenerative medicine.

Cite This Article

APA
Tamura N, Heidari N, Faragher RGA, Smith RKW, Dudhia J. (2023). Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells. J Equine Sci, 34(3), 67-72. https://doi.org/10.1294/jes.34.67

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 34
Issue: 3
Pages: 67-72

Researcher Affiliations

Tamura, Norihisa
  • Clinical Veterinary Medicine Division, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
  • Department of Clinical Sciences and Services, Royal Veterinary College, University of London, Hatfield AL9 7TA, UK.
Heidari, Neda
  • Department of Clinical Sciences and Services, Royal Veterinary College, University of London, Hatfield AL9 7TA, UK.
Faragher, Richard G A
  • School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton BN2 4GJ, UK.
Smith, Roger K W
  • Department of Clinical Sciences and Services, Royal Veterinary College, University of London, Hatfield AL9 7TA, UK.
Dudhia, Jayesh
  • Department of Clinical Sciences and Services, Royal Veterinary College, University of London, Hatfield AL9 7TA, UK.

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Citations

This article has been cited 1 times.
  1. Wątroba M, Szewczyk G, Szukiewicz D. The Role of Sirtuin-1 (SIRT1) in the Physiology and Pathophysiology of the Human Placenta. Int J Mol Sci 2023 Nov 11;24(22).
    doi: 10.3390/ijms242216210pubmed: 38003402google scholar: lookup
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