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International journal of molecular sciences2017; 18(10); doi: 10.3390/ijms18102122

The Contribution of Adipose Tissue-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma to the Treatment of Chronic Equine Laminitis: A Proof of Concept.

Abstract: Laminitis, a highly debilitating disease of the foot in ungulates, is characterized by pathological changes of the complex lamellar structures that maintain the appendicular skeleton within the hoof. Laminitis is a multifactorial disease that involves perturbation of the vascular, hematological, and inflammatory homeostasis of the foot. Interestingly, the pathogenesis of the disease resembles what is observed in metabolic syndromes and sepsis-induced organ failure in humans and animals. We hypothesized that local administration of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) might contribute to establishing an anti-inflammatory and pro-angiogenic environment, and could stimulate the injured tissue in order to restore its functional integrity. According to this assumption, an experimental protocol based on the local intravenous administration of adipose tissue-derived MSCs (aMSCs) in combination with PRP was developed for the treatment of horses affected by chronic laminitis. Nine horses with severely compromised venograms (showing grade III and IV laminitis) that had been unsuccessfully treated with conventional therapies were enrolled. aMSCs and PRP (15 × 10⁶ cells resuspended in 15 mL of PRP) were injected into the lateral or medial digital vein three times, at one-month intervals. The first administration was performed with allogeneic aMSCs, while for the following administrations, autologous aMSCs were used. There was no adverse short-term reaction to the intravenous injection of aMSCs. In the long term, venograms outlined, in all subjects, a progressive amelioration of the vascularization of the foot. An improvement in the structure and function of the hoof was also observed. No adverse events were reported during the follow-up, and the horses returned to a comfortable quality of life. Although the number of animals enrolled in the study is limited, both clinical observations and venography demonstrated an enhancement in the condition of all horses, suggesting that the regenerative therapies in chronic laminitis could be useful, and are worthy of further investigation.
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Publication Date: 2017-10-11 PubMed ID: 29019941PubMed Central: PMC5666804DOI: 10.3390/ijms18102122Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores how a treatment involving adipose tissue-derived mesenchymal stem cells (aMSCs) and platelet-rich plasma (PRP) can improve chronic laminitis, a serious foot disease in horses. Nine horses with advanced laminitis were treated over three months using this protocol, resulting in improved foot health and quality of life.

Deeper Understanding of the Research Paper

  • The paper presents an investigation into treating chronic laminitis, a debilitating disease affecting the feet of hoofed animals, in particular, horses. The disease is complex, involving the subversion of regular vascular, hematological, and inflammatory systems within the foot. This condition mirrors sepsis-induced organ failure and metabolic syndromes found in humans and other animals.
  • The research is based on the hypothesis that local delivery of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) could create an anti-inflammatory and pro-angiogenic environment, potentially stimulating tissue repair and recovery. The researchers propose a treatment protocol involving intravenous administration of adipose tissue-derived MSCs (aMSCs) in conjunction with PRP for horses suffering from chronic laminitis.

Methodology and Results

  • The study involved nine horses diagnosed with severe laminitis (grade III and IV) that hadn’t responded to traditional therapies. These horses underwent a treatment involving both aMSCs and PRP injections, segmented over three one-month intervals.
  • The first injection contained allogeneic (donor) aMSCs due to the time required to grow autologous (patient’s own) aMSCs, and the following injections were comprised of autologous aMSCs. All injections contained 15×10⁶ cells resuspended in 15ml of PRP.
  • The horses showed no short-term adverse reactions to the aMSCs injections. Long-term observation revealed a positive progression in foot vascularization and overall hoof structure/function. During the study and subsequent follow-up, the horses appeared to return to a high quality of life with no negative side effects reported.

Conclusion

  • Although there is a cautionary note that the sample size of the study was small, the alleviation of the condition in all the horses included strongly suggests that this treatment protocol shows promise. The researchers call for continued investigation into the benefits of such regenerative therapies in managing chronic laminitis in horses.

Cite This Article

APA
Angelone M, Conti V, Biacca C, Battaglia B, Pecorari L, Piana F, Gnudi G, Leonardi F, Ramoni R, Basini G, Dotti S, Renzi S, Ferrari M, Grolli S. (2017). The Contribution of Adipose Tissue-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma to the Treatment of Chronic Equine Laminitis: A Proof of Concept. Int J Mol Sci, 18(10). https://doi.org/10.3390/ijms18102122

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 18
Issue: 10

Researcher Affiliations

Angelone, Mario
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. marioangelonevet@libero.it.
Conti, Virna
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. virna.conti@unipr.it.
Biacca, Cristiano
  • Veterinary Practitioner, Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. cristiano.biacca@libero.it.
Battaglia, Beatrice
  • Veterinary Practitioner, Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. beatricechiarabattaglia@gmail.com.it.
Pecorari, Laura
  • Veterinary Practitioner, Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. laurapecorari.dvm@gmail.com.
Piana, Francesco
  • Veterinary Practitioner, Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. f.piana.vet@gmail.com.
Gnudi, Giacomo
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. giacomo.gnudi@unipr.it.
Leonardi, Fabio
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. fabio.leonardi@unipr.it.
Ramoni, Roberto
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. roberto.ramoni@unipr.it.
Basini, Giuseppina
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. giuseppina.basini@unipr.it.
Dotti, Silvia
  • Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy. silvia.dotti@izsler.it.
Renzi, Sabrina
  • Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy. sabrina.renzi@izsler.it.
Ferrari, Maura
  • Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy. maura1.ferrari@libero.it.
Grolli, Stefano
  • Dipartimento Scienze Mediche Veterinarie, Università di Parma, Via del taglio, 10, 43126 Parma, Italy. stefano.grolli@unipr.it.

MeSH Terms

  • Adipose Tissue / cytology
  • Administration, Intravenous
  • Animals
  • Chronic Disease
  • Foot Diseases / therapy
  • Foot Diseases / veterinary
  • Hoof and Claw / pathology
  • Horse Diseases / therapy
  • Horses
  • Inflammation / therapy
  • Inflammation / veterinary
  • Mesenchymal Stem Cell Transplantation
  • Mesenchymal Stem Cells / cytology
  • Platelet-Rich Plasma
  • Quality of Life
  • Regenerative Medicine

Conflict of Interest Statement

The authors declare no conflicts of interest.

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