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Home/Equine Research Bank/Int J Mol Sci/Equine Hoof Progenitor Cells Display Increased Mitochondrial...
International journal of molecular sciences2023; 24(14); doi: 10.3390/ijms241411446

Equine Hoof Progenitor Cells Display Increased Mitochondrial Metabolism and Adaptive Potential to a Highly Pro-Inflammatory Microenvironment.

Abstract: Medicinal signaling cells (MSC) exhibit distinct molecular signatures and biological abilities, depending on the type of tissue they originate from. Recently, we isolated and described a new population of stem cells residing in the coronary corium, equine hoof progenitor cells (HPCs), which could be a new promising cell pool for the treatment of laminitis. Therefore, this study aimed to compare native populations of HPCs to well-established adipose-derived stem cells (ASCs) in standard culture conditions and in a pro-inflammatory milieu to mimic a laminitis condition. ASCs and HPCs were either cultured in standard conditions or subjected to priming with a cytokines cocktail mixture. The cells were harvested and analyzed for expression of key markers for phenotype, mitochondrial metabolism, oxidative stress, apoptosis, and immunomodulation using RT-qPCR. The morphology and migration were assessed based on fluorescent staining. Microcapillary cytometry analyses were performed to assess the distribution in the cell cycle, mitochondrial membrane potential, and oxidative stress. Native HPCs exhibited a similar morphology to ASCs, but a different phenotype. The HPCs possessed lower migration capacity and distinct distribution across cell cycle phases. Native HPCs were characterized by different mitochondrial dynamics and oxidative stress levels. Under standard culture conditions, HPCs displayed different expression patterns of apoptotic and immunomodulatory markers than ASCs, as well as distinct miRNA expression. Interestingly, after priming with the cytokines cocktail mixture, HPCs exhibited different mitochondrial dynamics than ASCs; however, the apoptosis and immunomodulatory marker expression was similar in both populations. Native ASCs and HPCs exhibited different baseline expressions of markers involved in mitochondrial dynamics, the oxidative stress response, apoptosis and inflammation. When exposed to a pro-inflammatory microenvironment, ASCs and HPCs differed in the expression of mitochondrial condition markers and chosen miRNAs.
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Publication Date: 2023-07-14 PubMed ID: 37511204PubMed Central: PMC10379971DOI: 10.3390/ijms241411446Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigated the properties and potential therapeutic uses of stem cells found in the hooves of horses, known as hoof progenitor cells (HPCs). The study compared these cells with those derived from fat tissue and evaluated how they responded to inflammatory conditions that mirror laminitis, a painful disease in the hooves of horses.

Objective and Methodology

  • Researchers aimed to compare the biological characteristics and responses of HPCs and adipose-derived stem cells (ASCs) under standard conditions and in an environment simulating laminitis inflammation.
  • Following isolation, these cells were cultivated in standard conditions as well as inflammatory conditions using a mixture of cytokines, chemicals that coordinate cellular responses to inflammation and infection.
  • Cells were then examined for expression of specific markers to ascertain their phenotype, mitochondrial metabolism, oxidative stress, cell death (apoptosis), and immune modulation. Techniques like RT-qPCR, fluorescent staining, and microcapillary cytometry were used to analyze the cells.

Findings

  • HPCs showed similar morphology but different phenotype compared to ASCs. Histological differences included lower migration capacity and variations in cell cycle distributions for HPCs.
  • Researchers also observed that HPCs had unique mitochondrial dynamics and levels of oxidative stress.
  • Under standard conditions, HPCs exhibited different expression patterns of apoptotic and immunomodulatory markers than ASCs, as well as distinct miRNA expressions.
  • Inflammatory conditions led to changes in HPCs indicative of different mitochondrial responses compared to ASCs. Nevertheless, the expression levels of death and immune markers under these conditions were similar in both populations.

Conclusion

  • The study discovered that HPCs have unique cellular and molecular features when compared to ASCs. Their responses to inflammation were also unique, suggesting different susceptibilities and responses to laminitis.
  • The findings raise the possibility of using HPCs as a new source of stem cells for treating laminitis, meriting further investigation.

Cite This Article

APA
Pielok A, Kępska M, Steczkiewicz Z, Grobosz S, Bourebaba L, Marycz K. (2023). Equine Hoof Progenitor Cells Display Increased Mitochondrial Metabolism and Adaptive Potential to a Highly Pro-Inflammatory Microenvironment. Int J Mol Sci, 24(14). https://doi.org/10.3390/ijms241411446

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 24
Issue: 14

Researcher Affiliations

Pielok, Ariadna
  • Department of Experimental Biology, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 27B, 50-375 Wroclaw, Poland.
Kępska, Martyna
  • Department of Experimental Biology, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 27B, 50-375 Wroclaw, Poland.
Steczkiewicz, Zofia
  • Department of Experimental Biology, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 27B, 50-375 Wroclaw, Poland.
Grobosz, Sylwia
  • Department of Experimental Biology, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 27B, 50-375 Wroclaw, Poland.
Bourebaba, Lynda
  • Department of Experimental Biology, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 27B, 50-375 Wroclaw, Poland.
Marycz, Krzysztof
  • Department of Experimental Biology, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 27B, 50-375 Wroclaw, Poland.
  • International Institute of Translational Medicine, Jesionowa 11, Malin, 55-114 Wisznia Mała, Poland.

MeSH Terms

  • Animals
  • Horses
  • Adipose Tissue / metabolism
  • Mesenchymal Stem Cells / metabolism
  • Hoof and Claw
  • Stem Cells / metabolism
  • Cytokines / metabolism

Grant Funding

  • the Leading Research Groups support project from the subsidy increased for the period 2020-2025 in the amount of 2 % of the subsidy referred to Art. 387 (3) of the Law of 20 July 2018 on Higher Education and Science, obtained in 2019". / Wroclaw University of Environmental and Life Sciences

Conflict of Interest Statement

The authors declare no conflict of interest.

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