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Home / Equine Research Bank / Talanta / Electrochemical study of gelatin as a matrix for the immobil...
Talanta2010; 82(5); 1980-1985; doi: 10.1016/j.talanta.2010.08.019

Electrochemical study of gelatin as a matrix for the immobilization of horse heart cytochrome c.

Abstract: The aim of this paper is to emphasize the strength of gelatin as a stable matrix for redox enzymes. Cyclic voltammetry has been applied for a detailed electrochemical study of horse heart cytochrome c (HHC) entrapped in a gelatin matrix immobilized on a gold electrode. The influence of the HHC concentration, the mass percentage of the gelatin and the nature of the gelatin on the electrochemical behaviour of HHC have been described in detail. In addition, attenuated total reflection infrared (ATR-IR) spectroscopy was used to prove the immobilization on a qualitative and conformational level. The thickness of the gelatin film was determined using a non-contact optical profiler. These results open up new perspectives in the development of stable, biocompatible matrices for redox enzymes. The latter has its relevance in the field of biosensor development.
Copyright © 2010 Elsevier B.V. All rights reserved.
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Publication Date: 2010-08-24 PubMed ID: 20875605DOI: 10.1016/j.talanta.2010.08.019Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper investigates the ability of gelatin to serve as a durable matrix for the immobilization of redox enzymes, specifically horse heart cytochrome c, with potential applications in biosensor development.

Electrochemical Study

  • The researchers carried out an exhaustive electrochemical investigation on horse heart cytochrome c (HHC) encapsulated in a gelatin matrix immobilized on a gold electrode. This was done using a method known as cyclic voltammetry, which measures the current that develops in an electrochemical cell under conditions where voltage is varied in a systematic way.

Factors Influencing Electrochemical Behaviour

  • The study also detailed how HHC concentration, the mass percentage of the gelatin, and the type of gelatin used influence the electrochemical behaviour of HHC.

Confirmation of Immobilization

  • To verify the immobilization and understand the conformational level, attenuated total reflection infrared (ATR-IR) spectroscopy was employed. This spectroscopic technique is used in chemistry to study a thin film or fine particles. It reveals valuable information about the chemical composition and molecular orientation in the sample.

Determination of Gelatin Film Thickness

  • A non-contact optical profiler was utilized to determine the thickness of the gelatin film. This is an instrument used to measure the surface profiles of objects without physically touching the object. The data collected from this measurement can provide important information about the film’s physical properties.

Potential Applications in Biosensor Development

  • The results of this research point towards the potential of gelatin to be used as a stable and biocompatible matrix for redox enzymes. This has considerable importance in the field of biosensor development, where such enzymes can be used to convert a biological response into an electrical signal.

Cite This Article

APA
De Wael K, De Belder S, Van Vlierberghe S, Van Steenberge G, Dubruel P, Adriaens A. (2010). Electrochemical study of gelatin as a matrix for the immobilization of horse heart cytochrome c. Talanta, 82(5), 1980-1985. https://doi.org/10.1016/j.talanta.2010.08.019

Publication

Talanta
ISSN: 1873-3573
NlmUniqueID: 2984816R
Country: Netherlands
Language: English
Volume: 82
Issue: 5
Pages: 1980-1985

Researcher Affiliations

De Wael, Karolien
  • Department of Analytical Chemistry, Ghent University, Krijgslaan 281 S12, B-9000 Ghent, Belgium. Karolien.DeWael@UGent.be
De Belder, Stijn
    Van Vlierberghe, Sandra
      Van Steenberge, Geert
        Dubruel, Peter
          Adriaens, Annemie

            MeSH Terms

            • Animals
            • Biosensing Techniques / instrumentation
            • Biosensing Techniques / methods
            • Cytochromes c / chemistry
            • Electrochemical Techniques / instrumentation
            • Electrochemical Techniques / methods
            • Electrodes
            • Enzymes, Immobilized / chemistry
            • Gelatin / chemistry
            • Gold / chemistry
            • Horses
            • Hydrogels
            • Myocardium / enzymology
            • Spectrophotometry, Infrared
            • Surface Properties

            Citations

            This article has been cited 7 times.
            1. Samal SK, Soenen S, Puppi D, De Wael K, Pati S, De Smedt S, Braeckmans K, Dubruel P. Bio-Nanohybrid Gelatin/Quantum Dots for Cellular Imaging and Biosensing Applications. Int J Mol Sci 2022 Oct 6;23(19).
              doi: 10.3390/ijms231911867pubmed: 36233168google scholar: lookup
            2. Hofbauer S, Pignataro M, Borsari M, Bortolotti CA, Di Rocco G, Ravenscroft G, Furtmüller PG, Obinger C, Sola M, Battistuzzi G. Pseudoperoxidase activity, conformational stability, and aggregation propensity of the His98Tyr myoglobin variant: implications for the onset of myoglobinopathy. FEBS J 2022 Feb;289(4):1105-1117.
              doi: 10.1111/febs.16235pubmed: 34679218google scholar: lookup
            3. De Henau S, Tilleman L, Vangheel M, Luyckx E, Trashin S, Pauwels M, Germani F, Vlaeminck C, Vanfleteren JR, Bert W, Pesce A, Nardini M, Bolognesi M, De Wael K, Moens L, Dewilde S, Braeckman BP. A redox signalling globin is essential for reproduction in Caenorhabditis elegans. Nat Commun 2015 Dec 1;6:8782.
              doi: 10.1038/ncomms9782pubmed: 26621324google scholar: lookup
            4. Hamidi-Asl E, Dardenne F, Blust R, De Wael K. An improved electrochemical aptasensor for chloramphenicol detection based on aptamer incorporated gelatine. Sensors (Basel) 2015 Mar 27;15(4):7605-18.
              doi: 10.3390/s150407605pubmed: 25825978google scholar: lookup
            5. De Wael K, De Belder S, Pilehvar S, Van Steenberge G, Herrebout W, Heering HA. Enzyme-gelatin electrochemical biosensors: scaling down. Biosensors (Basel) 2012 Mar 15;2(1):101-13.
              doi: 10.3390/bios2010101pubmed: 25585635google scholar: lookup
            6. Tilleman L, De Henau S, Pauwels M, Nagy N, Pintelon I, Braeckman BP, De Wael K, Van Doorslaer S, Adriaensen D, Timmermans JP, Moens L, Dewilde S. An N-myristoylated globin with a redox-sensing function that regulates the defecation cycle in Caenorhabditis elegans. PLoS One 2012;7(12):e48768.
              doi: 10.1371/journal.pone.0048768pubmed: 23251335google scholar: lookup
            7. Xu HY, Chen ZW, Wu YM. Antitumor activity of total paeony glycoside against human chronic myelocytic leukemia K562 cell lines in vitro and in vivo. Med Oncol 2012 Jun;29(2):1137-47.
              doi: 10.1007/s12032-011-9909-9pubmed: 21452044google scholar: lookup
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