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Home / Equine Research Bank / Viruses / Emergence of Equine-like G3P[8] Rotavirus Strains Infecting ...
Viruses2025; 17(3); 410; doi: 10.3390/v17030410

Emergence of Equine-like G3P[8] Rotavirus Strains Infecting Children in Venezuela.

Abstract: is the leading cause of acute gastroenteritis worldwide in young humans and animals. In 2023-2024, a relatively high rotavirus detection rate (34.5%) was detected in children with diarrhea in Caracas. All rotavirus strains were typed as P[8], using a multiplex RT-PCR assay, while the G-type was not identified. This unusual pattern, not previously observed in Venezuela, prompted the VP7 gene sequencing of nineteen strains, which displayed a high sequence identity (99.3-100%) compatible with the G3 genotype. These strains clustered into a well-supported lineage IX encompassing human reassortants of equine-like G3P[8] strains described elsewhere, showing a very close genetic relationship (99.0-99.9%). Old G3 rotavirus isolates obtained from diarrheic samples in the past were included in the analysis and grouped into lineage I together with ancestral reference G3 strains. The novel G3P[8]s carry amino acid changes in VP7-neutralizing epitopes, compared with the RotaTeq-WI78-8-vaccine strain. Full genome sequencing of a representative strain revealed a genotype constellation including an equine-like G3P[8] in a DS-1-like backbone (I2-R2-C2-M2-A2-N2-T2-E2-H2), confirming the role of animal strains as a source of diversification, and the importance of unceasingly revising molecular typing strategies and vaccine efficacy to guarantee their success.
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Publication Date: 2025-03-13 PubMed ID: 40143336PubMed Central: PMC11946648DOI: 10.3390/v17030410Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored the emergence of an equine-like strain of rotavirus infecting children in Venezuela. In 2023-2024, there was a high rate of rotavirus detection, and they discovered that the strains were of a type not previously observed in the region.

Identification of the Rotavirus Strain

  • The research ostensibly focused on an unusually high detection rate of acute gastroenteritis in children in Caracas, Venezuela, caused by the rotavirus.
  • RotaVirus is the major cause of gastroenteritis in young humans Worldwide.
  • Detection rates rose to 34.5% thus prompting investigations into the strain causing the outbreak.
  • Through multiplex RT-PCR assays, researchers identified all the rotavirus strains as P[8] type, but the G-type could not be identified.

Detailed Analysis into the Strains

  • An in-depth analysis through VP7 gene sequencing in nineteen strains was conducted due to the unusual pattern of the strains
  • The strains revealed a high sequence identity (99.3-100%) in line with the G3 genotype.
  • The characterised strains formed a well-established line IX, matching human reassortments of equine-like G3P[8] strains reported elsewhere.
  • These strains demonstrated a very close genetic relationship (99.0-99.9%) with previous strains.

Past vs New Strains

  • For comparative purposes, older G3 rotavirus strains were included in the study.
  • The older strains and some reference G3 strains grouped in lineage I, separate from the emergent strains.

Characteristics of Novel G3P[8] Strains

  • Compared with the RotaTeq-WI78-8-vaccine strain, the novel G3P[8]s harbor different amino acid compositions in VP7-neutralizing epitopes.
  • Full genome sequencing of a representative strain revealed a genotype constellation, suggesting the possible role of animal strains in diversification.

Implications for Molecular Typing and Vaccine Efficacy

  • This study underscores the crucial role of continuously reviewing molecular typing strategies and vaccine efficacy.
  • The emergent strains’ distinctive characteristics hint at the need for modifications to guarantee the effectiveness of vaccine programs.

Cite This Article

APA
(2025). Emergence of Equine-like G3P[8] Rotavirus Strains Infecting Children in Venezuela. Viruses, 17(3), 410. https://doi.org/10.3390/v17030410

Publication

Viruses
ISSN: 1999-4915
NlmUniqueID: 101509722
Country: Switzerland
Language: English
Volume: 17
Issue: 3
PII: 410

Researcher Affiliations

MeSH Terms

  • Rotavirus / genetics
  • Rotavirus / classification
  • Rotavirus / isolation & purification
  • Rotavirus Infections / virology
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / veterinary
  • Venezuela / epidemiology
  • Humans
  • Phylogeny
  • Genotype
  • Animals
  • Genome, Viral
  • Child, Preschool
  • Diarrhea / virology
  • Diarrhea / veterinary
  • Diarrhea / epidemiology
  • Infant
  • Capsid Proteins / genetics
  • Horses / virology
  • Child
  • Antigens, Viral / genetics
  • Gastroenteritis / virology
  • Gastroenteritis / epidemiology
  • Female
  • Reassortant Viruses / genetics
  • Reassortant Viruses / classification
  • Reassortant Viruses / isolation & purification
  • Feces / virology
  • Male

Grant Funding

  • 20230PGP53 / Fondo Nacional de Ciencia Tecnologu00eda e Innovaciu00f3n

Conflict of Interest Statement

The authors declare no conflicts of interest.

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