FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Viruses / Equid alphaherpesvirus 1 from Italian Horses: Evaluation of ...
Viruses2019; 11(9); 851; doi: 10.3390/v11090851

Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes.

Abstract: This study investigates the genetic variability of the equid alphaherpesvirus type 1 (EHV-1), a virus in horses, by evaluating four specific genes (ORF30, ORF33, ORF34, and ORF68) in Italian horse […]
Get Full Text
Publication Date: 2019-09-13 PubMed ID: 31540321PubMed Central: PMC6784080DOI: 10.3390/v11090851Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the genetic variability of the equid alphaherpesvirus type 1 (EHV-1), a virus in horses, by evaluating four specific genes (ORF30, ORF33, ORF34, and ORF68) in Italian horse populations.

Virus and Genes Studied

  • The research explores the equid alphaherpesvirus type 1 (EHV-1), a virus that infects horses and can cause various health problems including respiratory disease, abortion and a neurologic disease.
  • The focus of this study is on the variability in four genes of EHV-1: ORF30, ORF33, ORF34, and ORF68. These genes were selected because variants in these genes have been found to affect the severity and type of disease caused by EHV-1.

Methodology

  • The researchers collected samples from Italian horses infected with EHV-1.
  • They carried out sequence analysis on the genes ORF30, ORF33, ORF34, and ORF68 in these virus samples.
  • Next, they compared the sequences of these genes to those of known EHV-1 viruses to identify any mutations or variants.

Results and Implications

  • The exact results of the analysis would depend on the data discovered, which is not given in this abstract. However, the aim was to characterize the variability in these crucial EHV-1 genes in Italian horses.
  • Such information could be important for understanding the behavior of EHV-1 in different horse populations and could possibly lead to the development of improved diagnostic tests or vaccines. It could also play a fundamental role in designing and implementing targeted measures for the control and prevention of diseases caused by this virus in horses.

Cite This Article

APA
Preziuso S, Sgorbini M, Marmorini P, Cuteri V. (2019). Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes. Viruses, 11(9), 851. https://doi.org/10.3390/v11090851

Publication

Viruses
ISSN: 1999-4915
NlmUniqueID: 101509722
Country: Switzerland
Language: English
Volume: 11
Issue: 9
PII: 851

Researcher Affiliations

Preziuso, Silvia
  • School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica (MC), Italy. silvia.preziuso@unicam.it.
Sgorbini, Micaela
  • Department of Veterinary Sciences, University of Pisa, San Piero a Grado, 56122 Pisa, Italy. micaela.sgorbini@unipi.it.
Marmorini, Paola
  • Private Practitioner, 52100 Arezzo, Italy. ninnaro@tiscali.it.
Cuteri, Vincenzo
  • School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica (MC), Italy. vincenzo.cuteri@unicam.it.

MeSH Terms

  • Animals
  • DNA, Viral / genetics
  • Databases, Nucleic Acid
  • Genetic Variation
  • Genotype
  • Herpesviridae Infections / veterinary
  • Herpesvirus 1, Equid / classification
  • Herpesvirus 1, Equid / genetics
  • Horse Diseases / virology
  • Horses / virology
  • Italy
  • Open Reading Frames
  • Polymorphism, Single Nucleotide
  • Sequence Analysis, DNA

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 36 references
  1. OIE-Listed Diseases, Infections and Infestations in Force in 2019. [(accessed on 27 July 2019)]; Available online: www.oie.int.
  2. Telford EA, Watson MS, McBride K, Davison AJ. The DNA sequence of equine herpesvirus-1.. Virology 1992 Jul;189(1):304-16.
    doi: 10.1016/0042-6822(92)90706-Upubmed: 1318606google scholar: lookup
  3. OIE. Equine rhinopneumonitis (infection with equid herpesvirus-1 and -4). OIE Terrestrial Manual 2017; Chapter 2.5.9.
  4. Nugent J, Birch-Machin I, Smith KC, Mumford JA, Swann Z, Newton JR, Bowden RJ, Allen GP, Davis-Poynter N. Analysis of equid herpesvirus 1 strain variation reveals a point mutation of the DNA polymerase strongly associated with neuropathogenic versus nonneuropathogenic disease outbreaks.. J Virol 2006 Apr;80(8):4047-60.
    doi: 10.1128/JVI.80.8.4047-4060.2006pmc: PMC1440451pubmed: 16571821google scholar: lookup
  5. Allen GP, Breathnach CC. Quantification by real-time PCR of the magnitude and duration of leucocyte-associated viraemia in horses infected with neuropathogenic vs. non-neuropathogenic strains of EHV-1.. Equine Vet J 2006 May;38(3):252-7.
    doi: 10.2746/042516406776866453pubmed: 16706281google scholar: lookup
  6. Goodman LB, Loregian A, Perkins GA, Nugent J, Buckles EL, Mercorelli B, Kydd JH, Palù G, Smith KC, Osterrieder N, Davis-Poynter N. A point mutation in a herpesvirus polymerase determines neuropathogenicity.. PLoS Pathog 2007 Nov;3(11):e160.
    doi: 10.1371/journal.ppat.0030160pmc: PMC2065875pubmed: 17997600google scholar: lookup
  7. Malik P, Bálint A, Dán A, Pálfi V. Molecular characterisation of the ORF68 region of equine herpesvirus-1 strains isolated from aborted fetuses in Hungary between 1977 and 2008.. Acta Vet Hung 2012 Mar;60(1):175-87.
    doi: 10.1556/AVet.2012.015pubmed: 22366142google scholar: lookup
  8. Anagha G, Gulati BR, Riyesh T, Virmani N. Genetic characterization of equine herpesvirus 1 isolates from abortion outbreaks in India.. Arch Virol 2017 Jan;162(1):157-163.
    doi: 10.1007/s00705-016-3097-zpubmed: 27699511google scholar: lookup
  9. Negussie H, Gizaw D, Tessema TS, Nauwynck HJ. Equine Herpesvirus-1 Myeloencephalopathy, an Emerging Threat of Working Equids in Ethiopia.. Transbound Emerg Dis 2017 Apr;64(2):389-397.
    doi: 10.1111/tbed.12377pubmed: 26010868google scholar: lookup
  10. Stasiak K, Dunowska M, Hills SF, Rola J. Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland.. Arch Virol 2017 Aug;162(8):2329-2335.
    doi: 10.1007/s00705-017-3376-3pmc: PMC5506511pubmed: 28451902google scholar: lookup
  11. Matczuk AK, Skarbek M, Jackulak NA, Bażanów BA. Molecular characterisation of equid alphaherpesvirus 1 strains isolated from aborted fetuses in Poland.. Virol J 2018 Dec 3;15(1):186.
    doi: 10.1186/s12985-018-1093-5pmc: PMC6276253pubmed: 30509297google scholar: lookup
  12. Garvey M, Lyons R, Hector RD, Walsh C, Arkins S, Cullinane A. Molecular Characterisation of Equine Herpesvirus 1 Isolates from Cases of Abortion, Respiratory and Neurological Disease in Ireland between 1990 and 2017.. Pathogens 2019 Jan 15;8(1).
    doi: 10.3390/pathogens8010007pmc: PMC6471309pubmed: 30650561google scholar: lookup
  13. Bryant NA, Wilkie GS, Russell CA, Compston L, Grafham D, Clissold L, McLay K, Medcalf L, Newton R, Davison AJ, Elton DM. Genetic diversity of equine herpesvirus 1 isolated from neurological, abortigenic and respiratory disease outbreaks.. Transbound Emerg Dis 2018 Jun;65(3):817-832.
    doi: 10.1111/tbed.12809pmc: PMC5947664pubmed: 29423949google scholar: lookup
  14. Autorino GL, Corradi V, Frontoso R, Galletti S, Manna G, Mascioni A, Pallone A, Ricci I, Rosone F, Simula M. P.3 Gestione di un focolaio neurologico da Equine herpesvirus 1 (EHV-1). Workshop Nazionale di Virologia Veterinaria 2014; p. 15.
  15. Preziuso S, Cuteri V. A Multiplex Polymerase Chain Reaction Assay for Direct Detection and Differentiation of β-Hemolytic Streptococci in Clinical Samples from Horses. J. Equine Vet. Sci. 2012;32:292–296.
    doi: 10.1016/j.jevs.2011.11.001google scholar: lookup
  16. Preziuso S, Pinho MD, Attili AR, Melo-Cristino J, Acke E, Midwinter AC, Cuteri V, Ramirez M. PCR based differentiation between Streptococcus dysgalactiae subsp. equisimilis strains isolated from humans and horses.. Comp Immunol Microbiol Infect Dis 2014 May;37(3):169-72.
    doi: 10.1016/j.cimid.2014.04.001pubmed: 24813401google scholar: lookup
  17. Wang L, Raidal SL, Pizzirani A, Wilcox GE. Detection of respiratory herpesviruses in foals and adult horses determined by nested multiplex PCR.. Vet Microbiol 2007 Mar 31;121(1-2):18-28.
    doi: 10.1016/j.vetmic.2006.11.009pubmed: 17208393google scholar: lookup
  18. Allen GP. Antemortem detection of latent infection with neuropathogenic strains of equine herpesvirus-1 in horses.. Am J Vet Res 2006 Aug;67(8):1401-5.
    doi: 10.2460/ajvr.67.8.1401pubmed: 16881853google scholar: lookup
  19. Untergasser A, Nijveen H, Rao X, Bisseling T, Geurts R, Leunissen JA. Primer3Plus, an enhanced web interface to Primer3.. Nucleic Acids Res 2007 Jul;35(Web Server issue):W71-4.
    doi: 10.1093/nar/gkm306pmc: PMC1933133pubmed: 17485472google scholar: lookup
  20. Hall TA. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 1999;41:95–98.
  21. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput.. Nucleic Acids Res 2004;32(5):1792-7.
    doi: 10.1093/nar/gkh340pmc: PMC390337pubmed: 15034147google scholar: lookup
  22. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.. Mol Biol Evol 2016 Jul;33(7):1870-4.
    doi: 10.1093/molbev/msw054pmc: PMC8210823pubmed: 27004904google scholar: lookup
  23. Mori E, Lara Mdo C, Cunha EM, Villalobos EM, Mori CM, Soares RM, Brandão PE, Fernandes WR, Richtzenhain LJ. Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers.. Braz J Microbiol 2015 Jun;46(2):565-70.
    doi: 10.1590/S1517-838246220140096pmc: PMC4507552pubmed: 26273275google scholar: lookup
  24. Turan N, Yildirim F, Altan E, Sennazli G, Gurel A, Diallo I, Yilmaz H. Molecular and pathological investigations of EHV-1 and EHV-4 infections in horses in Turkey.. Res Vet Sci 2012 Dec;93(3):1504-7.
    doi: 10.1016/j.rvsc.2012.01.019pubmed: 22401978google scholar: lookup
  25. Perkins GA, Goodman LB, Tsujimura K, Van de Walle GR, Kim SG, Dubovi EJ, Osterrieder N. Investigation of the prevalence of neurologic equine herpes virus type 1 (EHV-1) in a 23-year retrospective analysis (1984-2007).. Vet Microbiol 2009 Nov 18;139(3-4):375-8.
    doi: 10.1016/j.vetmic.2009.06.033pubmed: 19615831google scholar: lookup
  26. Vissani MA, Becerra ML, Olguín Perglione C, Tordoya MS, Miño S, Barrandeguy M. Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina.. Vet Microbiol 2009 Nov 18;139(3-4):361-4.
    doi: 10.1016/j.vetmic.2009.06.025pubmed: 19589651google scholar: lookup
  27. Pronost S, Léon A, Legrand L, Fortier C, Miszczak F, Freymuth F, Fortier G. Neuropathogenic and non-neuropathogenic variants of equine herpesvirus 1 in France.. Vet Microbiol 2010 Oct 26;145(3-4):329-33.
    doi: 10.1016/j.vetmic.2010.03.031pubmed: 20427133google scholar: lookup
  28. Smith KL, Allen GP, Branscum AJ, Frank Cook R, Vickers ML, Timoney PJ, Balasuriya UB. The increased prevalence of neuropathogenic strains of EHV-1 in equine abortions.. Vet Microbiol 2010 Feb 24;141(1-2):5-11.
    doi: 10.1016/j.vetmic.2009.07.030pubmed: 19733451google scholar: lookup
  29. Fritsche AK, Borchers K. Detection of neuropathogenic strains of Equid Herpesvirus 1 (EHV-1) associated with abortions in Germany.. Vet Microbiol 2011 Jan 10;147(1-2):176-80.
    doi: 10.1016/j.vetmic.2010.06.014pubmed: 20619972google scholar: lookup
  30. Tsujimura K, Oyama T, Katayama Y, Muranaka M, Bannai H, Nemoto M, Yamanaka T, Kondo T, Kato M, Matsumura T. Prevalence of equine herpesvirus type 1 strains of neuropathogenic genotype in a major breeding area of Japan.. J Vet Med Sci 2011 Dec;73(12):1663-7.
    doi: 10.1292/jvms.11-0140pubmed: 21828961google scholar: lookup
  31. Castro ER, Arbiza J. Detection and genotyping of equid herpesvirus 1 in Uruguay.. Rev Sci Tech 2017 Dec;36(3):799-806.
    doi: 10.20506/rst.36.3.2715pubmed: 30160700google scholar: lookup
  32. Damiani AM, de Vries M, Reimers G, Winkler S, Osterrieder N. A severe equine herpesvirus type 1 (EHV-1) abortion outbreak caused by a neuropathogenic strain at a breeding farm in northern Germany.. Vet Microbiol 2014 Aug 27;172(3-4):555-62.
    doi: 10.1016/j.vetmic.2014.06.023pubmed: 25042527google scholar: lookup
  33. Vaz PK, Horsington J, Hartley CA, Browning GF, Ficorilli NP, Studdert MJ, Gilkerson JR, Devlin JM. Evidence of widespread natural recombination among field isolates of equine herpesvirus 4 but not among field isolates of equine herpesvirus 1.. J Gen Virol 2016 Mar;97(3):747-755.
    doi: 10.1099/jgv.0.000378pmc: PMC5381393pubmed: 26691326google scholar: lookup
  34. Guo X, Izume S, Okada A, Ohya K, Kimura T, Fukushi H. Full genome sequences of zebra-borne equine herpesvirus type 1 isolated from zebra, onager and Thomson's gazelle.. J Vet Med Sci 2014 Sep;76(9):1309-12.
    doi: 10.1292/jvms.14-0183pmc: PMC4197165pubmed: 24920546google scholar: lookup
  35. Said A, Damiani A, Osterrieder N. Ubiquitination and degradation of the ORF34 gene product of equine herpesvirus type 1 (EHV-1) at late times of infection.. Virology 2014 Jul;460-461:11-22.
    doi: 10.1016/j.virol.2014.05.009pubmed: 25010266google scholar: lookup
  36. Shakya AK, O'Callaghan DJ, Kim SK. Comparative Genomic Sequencing and Pathogenic Properties of Equine Herpesvirus 1 KyA and RacL11.. Front Vet Sci 2017;4:211.
    doi: 10.3389/fvets.2017.00211pmc: PMC5732242pubmed: 29312962google scholar: lookup

Citations

This article has been cited 7 times.
Subscribe to our newsletter
Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.