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BMC veterinary research2016; 12(1); 254; doi: 10.1186/s12917-016-0880-8

Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum.

Abstract: Dendritic cells are professional antigen-presenting cells that play an essential role in the initiation and modulation of T cell responses. They have been studied widely for their potential clinical applications, but for clinical use to be successful, alternatives to xenogeneic substances like fetal bovine serum (FBS) in cell culture need to be found. Protocols for the generation of dendritic cells ex vivo from monocytes are well established for several species, including horses. Currently, the gold standard protocol for generating dendritic cells from monocytes across various species relies upon a combination of GM-CSF and IL-4 added to cell culture medium which is supplemented with FBS. The aim of this study was to substitute FBS with heterologous horse serum. For this purpose, equine monocyte-derived dendritic cells (eqMoDC) were generated in the presence of horse serum or FBS and analysed for the effect on morphology, phenotype and immunological properties. Changes in the expression of phenotypic markers (CD14, CD86, CD206) were assessed during dendritic cell maturation by flow cytometry. To obtain a more complete picture of the eqMoDC differentiation and assess possible differences between FBS- and horse serum-driven cultures, a transcriptomic microarray analysis was performed. Lastly, immature eqMoDC were primed with a primary antigen (ovalbumin) or a recall antigen (tetanus toxoid) and, after maturation, were co-cultured with freshly isolated autologous CD5 T lymphocytes to assess their T cell stimulatory capacity. Results: The microarray analysis demonstrated that eqMoDC generated with horse serum were indistinguishable from those generated with FBS. However, eqMoDC incubated with horse serum-supplemented medium exhibited a more characteristic dendritic cell morphology during differentiation from monocytes. A significant increase in cell viability was also observed in eqMoDC cultured with horse serum. Furthermore, eqMoDC generated in the presence of horse serum were found to be superior in their functional T lymphocyte priming capacity and to elicit significantly less non-specific proliferation. Conclusions: EqMoDC generated with horse serum-supplemented medium showed improved morphological characteristics, higher cell viability and exhibited a more robust performance in the functional T cell assays. Therefore, horse serum was found to be superior to FBS for generating equine monocyte-derived dendritic cells.
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Publication Date: 2016-11-15 PubMed ID: 27846835PubMed Central: PMC5111218DOI: 10.1186/s12917-016-0880-8Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the efficacy of using horse serum versus fetal bovine serum (FBS) in generating dendritic cells, with results indicating that horse serum is superior to FBS when it comes to the creation and function of dendritic cells derived from monocytes in horses.

Overview of the Research

  • The research was conducted with the aim of comparing dendritic cells generated using horse serum versus those generated using fetal bovine serum (FBS).
  • Dendritic cells play a crucial role in kickstarting and modulating T cell responses, thus making them a significant area of study for potential clinical applications.
  • The scientists pointed out that for clinical applications to be successful, alternatives to xenogeneic substances like FBS needed to be explored.
  • The dendritic cells used in this study were produced ex vivo from monocytes, a method which is established across several species, including horses.
  • The team compared the effects of horse serum and FBS on the morphology, phenotype, and immunological properties of the dendritic cells.

Findings of the Study

  • The morphological changes of the dendritic cells were more characteristic when developed with horse serum, and cell viability also increased, compared to those produced with FBS.
  • Results from the transcriptomic microarray analysis showed that dendritic cells generated with either horse serum or FBS couldn’t be differentiated based on their transcriptomic information.
  • The functionality of the dendritic cells was also observed by priming immature cells with primary and recall antigens, after which they were co-cultured with T lymphocytes to evaluate their T cell stimulatory capacity.
  • It was found that dendritic cells generated with horse serum demonstrated superior capacity to prime T lymphocytes and significantly less non-specific proliferation.

Conclusions from the Study

  • The researchers concluded that horse serum-supplemented medium proves to be superior to FBS in generating dendritic cells from equine monocytes.
  • The dendritic cells produced with horse serum demonstrated improved morphological characteristics, enhanced cell viability, and superior performance in T cell assays.

Cite This Article

APA
Ziegler A, Everett H, Hamza E, Garbani M, Gerber V, Marti E, Steinbach F. (2016). Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum. BMC Vet Res, 12(1), 254. https://doi.org/10.1186/s12917-016-0880-8

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 12
Issue: 1
Pages: 254
PII: 254

Researcher Affiliations

Ziegler, Anja
  • Department of Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, 3001, Bern, Switzerland. anja.ziegler@vetsuisse.unibe.ch.
Everett, Helen
  • Virology Department, Animal and Plant Health Agency-Weybridge, Woodham Lane, Addlestone, Surrey, KT15 3NB, UK.
Hamza, Eman
  • Department of Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, 3001, Bern, Switzerland.
  • Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Garbani, Mattia
  • Swiss Institute of Allergy and Asthma Research (SIAF), University of Zürich, Davos, Switzerland.
Gerber, Vinzenz
  • Swiss Institute of Equine Medicine, University of Bern, Bern, Switzerland.
Marti, Eliane
  • Department of Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, 3001, Bern, Switzerland.
Steinbach, Falko
  • Faculty of Health and Medical Sciences, School of Veterinary Medicine, University of Surrey, Daphne Jackson Road, Guildford, GU2 7AL, UK. f.steinbach@surrey.ac.uk.

MeSH Terms

  • Animals
  • Cattle
  • Cell Culture Techniques / methods
  • Cell Differentiation / drug effects
  • Cell Differentiation / genetics
  • Cell Survival / drug effects
  • Cells, Cultured
  • Culture Media / chemistry
  • Culture Media / pharmacology
  • Culture Media / standards
  • Dendritic Cells / cytology
  • Dendritic Cells / drug effects
  • Gene Expression Profiling / veterinary
  • Gene Expression Regulation / drug effects
  • Horses
  • Monocytes / cytology
  • Serum / metabolism

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This article has been cited 7 times.
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