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Home / Equine Research Bank / Arch Virol / Equine herpesvirus type 1 (EHV-1)-induced rearrangements of ...
Archives of virology2013; 159(6); 1341-1349; doi: 10.1007/s00705-013-1949-3

Equine herpesvirus type 1 (EHV-1)-induced rearrangements of actin filaments in productively infected primary murine neurons.

Abstract: Equine herpesvirus type 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. In the present study, we investigated reorganization of the cytoskeleton in neurons infected with two EHV-1 strains: Jan-E (wild-type strain) and Rac-H (attenuated strain). The studies were performed on primary murine neurons, which are an excellent model for studying neurotropism and neurovirulence of EHV-1. We have demonstrated for the first time that EHV-1 infection causes rearrangements in the actin network of neurons that are dependent on the virus strain and its adaptation to cell culture in vitro. Immunofluorescent labeling and confocal microscopy revealed the formation of long, thin projections in neurons infected with the Jan-E strain, which was probably associated with enhanced intracellular spread of the virus. The EHV-1 Rac-H strain caused disruption of the microfilaments system and general depolymerization of actin, but treatment of neurons with cytochalasin D or latrunculin A resulted in limitation of viral replication. It can therefore be assumed that actin filaments are required only at the early stages of infection. Our results allow us to suggest that the actin cytoskeleton participates in EHV-1 infection of primary murine neurons but is not essential, and that other components of the cytoskeleton and/or cellular mechanisms may be also involved during EHV-1 infection.
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Publication Date: 2013-12-19 PubMed ID: 24352436PubMed Central: PMC4042010DOI: 10.1007/s00705-013-1949-3Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses how the Equine herpesvirus type 1 (EHV-1) affects the actin filaments in primary murine neurons, causing respiratory disease, abortion, and neurological disorders in horses. The effects vary according to the virus strain and some changes can be limited by certain treatments.

Overview of the Research

  • The research is centered on understanding the impact of EHV-1 infection on the arrangement of actin filaments (the skeletal framework inside cell cytoplasm) in the neurons of mice. The researchers conducted their study on two strains of EHV-1, namely Jan-E (wild-type strain) and Rac-H (attenuated strain).
  • The study model involved primary murine neurons, an optimal choice given their relevance for studying the tendency of EHV-1 towards nerves (neurotropism) and the severity of the disease (neurovirulence).

Main Findings

  • The research noticed for the first time that an EHV-1 infection can cause significant changes in the actin network of neurons. This depends largely on the type of virus strain and its adjustment to cell culture in vitro.
  • Through immunofluorescent marking techniques and advanced microscopy, long, thin projections were observed in neurons infected with the Jan-E strain. The researchers suggest that this could be linked to the increased spread of the virus within the cell.
  • The Rac-H strain of EHV-1 led to the destruction of the microfilaments system and widespread breakdown of actin. However, when the neurons were treated with substances like cytochalasin D or latrunculin A, the replication of the virus was restricted.
  • Thus, it is suggested that actin filaments are essential during the initial stages of infection.

Implications

  • The results implicate an active role of the actin cytoskeleton in the infection of primary murine neurons by EHV-1. However, they also note that the actin cytoskeleton is not indispensable to the process.
  • Thus, the research suggests that other elements of the cytoskeleton or other cellular mechanics could also contribute to the progression of EHV-1 infection.

Cite This Article

APA
Słońska A, Cymerys J, Godlewski MM, Dzieciątkowski T, Tucholska A, Chmielewska A, Golke A, Bańbura MW. (2013). Equine herpesvirus type 1 (EHV-1)-induced rearrangements of actin filaments in productively infected primary murine neurons. Arch Virol, 159(6), 1341-1349. https://doi.org/10.1007/s00705-013-1949-3

Publication

Archives of virology
ISSN: 1432-8798
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 159
Issue: 6
Pages: 1341-1349

Researcher Affiliations

Słońska, A
  • Division of Microbiology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, SGGW, Ciszewskiego 8, 02-786, Warsaw, Poland, anex007@op.pl.
Cymerys, J
    Godlewski, M M
      Dzieciątkowski, T
        Tucholska, A
          Chmielewska, A
            Golke, A
              Bańbura, M W

                MeSH Terms

                • Actin Cytoskeleton / metabolism
                • Animals
                • Cells, Cultured
                • Herpesvirus 1, Equid / growth & development
                • Herpesvirus 1, Equid / physiology
                • Host-Pathogen Interactions
                • Mice
                • Neurons / virology

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                Citations

                This article has been cited 5 times.
                1. Chodkowski M, Słońska A, Gregorczyk-Zboroch K, Nowak-Zyczynska Z, Golke A, Krzyżowska M, Bańbura MW, Cymerys J. Equid Alphaherpesvirus 1 (EHV-1) Influences Morphology and Function of Neuronal Mitochondria In Vitro.. Pathogens 2022 Aug 3;11(8).
                  doi: 10.3390/pathogens11080876pubmed: 36014997google scholar: lookup
                2. Bartak M, Chodkowski M, Słońska A, Grodzik M, Szczepaniak J, Bańbura MW, Cymerys J. Equid Alphaherpesvirus 1 Modulates Actin Cytoskeleton and Inhibits Migration of Glioblastoma Multiforme Cell Line A172.. Pathogens 2022 Mar 25;11(4).
                  doi: 10.3390/pathogens11040400pubmed: 35456075google scholar: lookup
                3. Słońska A, Cymerys J, Chodkowski M, Bąska P, Krzyżowska M, Bańbura MW. Human herpesvirus type 2 infection of primary murine astrocytes causes disruption of the mitochondrial network and remodeling of the actin cytoskeleton: an in vitro morphological study.. Arch Virol 2021 May;166(5):1371-1383.
                  doi: 10.1007/s00705-021-05025-xpubmed: 33715038google scholar: lookup
                4. Denes CE, Miranda-Saksena M, Cunningham AL, Diefenbach RJ. Cytoskeletons in the Closet-Subversion in Alphaherpesvirus Infections.. Viruses 2018 Feb 13;10(2).
                  doi: 10.3390/v10020079pubmed: 29438303google scholar: lookup
                5. Cymerys J, Słońska A, Tucholska A, Golke A, Chmielewska A, Bańbura MW. Influence of long-term equine herpesvirus type 1 (EHV-1) infection on primary murine neurons-the possible effects of the multiple passages of EHV-1 on its neurovirulence.. Folia Microbiol (Praha) 2018 Jan;63(1):1-11.
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