Erratum to: Viability of equine mesenchymal stem cells during transport and implantation.

The research focuses on the viability of equine mesenchymal stem cells during transport and implantation. It particularly looks into how storage and injection techniques affect the stem cells’ ability to survive, grow, and behave, recommending that the cells should be implanted within 24 hours of recovery using larger needle bores for better results.

Introduction

• The research begins with a mention of how injecting mesenchymal stem cells (MSC), which are self-generated or autologous, into horse tendon injuries has proven to be safe and effective. Studies in this area are helping to develop a method to translate this technique for use in humans.
• To successfully move these cells from the lab to the tissue requires specific and validated transport and implantation techniques.

Methods

• The researchers carried out tests on cell survivability in diverse media for a span of 72 hours and after injection through needles of varying thickness sizes (19G, 21G or 23G).
• They utilized TrypanBlue, alamarBlue®, and AnnexinV assays to analyze cell viability, growth, and apoptosis (programmed cell death).

Results

• The results revealed that the Neil viability was the same in all re-suspension media after 24 hours of storage. However, cell death was most rapid in bone marrow aspirate, platelet-rich plasma, and serum after extended storage.
• Cryogenic media showed the highest cell viability, regardless of how long they were stored.
• Cell proliferation after 24 and 72 hours of storage was the same for all media, except for serum. Cells stored in serum showed enhanced proliferation after 24 hours.
• The ability of MSCs to differentiate into three distinct lineages and their viability did not significantly alter when passed through 19G, 21G, or 23G needles. But, injecting through 21G and 23G needles significantly increased the number of apoptotic cells compared to those passed through a 19G needle and those not injected.
• After the injection, all gauges caused a temporary drop in cell metabolic activity but it recovered by 2 hours.

Conclusions

• The research concludes by highlighting that storage and injection methods significantly impact the viability and behaviour of the stem cells.
• They recommend that for better therapy outcomes, the cells should be implanted within 24 hours of recovery from the culture. They also recommended using larger needle bores.