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Home / Equine Research Bank / Pflugers Arch / Estimating transit time for capillary blood in selected musc...
Pflugers Archiv : European journal of physiology1992; 421(6); 578-584; doi: 10.1007/BF00375054

Estimating transit time for capillary blood in selected muscles of exercising animals.

Abstract: The mean minimal capillary transit time was estimated in muscles of various animals using a combination of physiological and morphometric methods. Radioactive microspheres were injected intravascularly in various animals running on a treadmill at maximum oxygen consumption rate (VO2,max) to label blood flow to individual muscles. The muscles were then removed and preserved by standard methods for electron microscopy. The volume density of mitochondria was measured to assess muscle oxidative capacity. Capillary densities in muscle cross-sections, capillary diameters and tortuosities were incorporated into an estimate of capillary volume per unit muscle mass. Mean capillary transit time (tc) in the exercising muscles was estimated by dividing mass-specific capillary volume by mass-specific blood flow. Estimates of tc ranged from values near 1 s in horse heart and thigh muscles to 0.2 s in duck gastrocnemius. The relationship between muscle blood flow and tc was hyperbolic. The experimental data indicate a limiting value of 0.2 s for transit times at very high blood flows. There was no correlation between tc and body-mass-specific VO2,max.
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Publication Date: 1992-09-01 PubMed ID: 1437519DOI: 10.1007/BF00375054Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the estimate of the average minimum capillary transit time in the muscles of various exercising animals using physiological and morphometric methods, including the use of radioactive microspheres and electron microscopy.

Research Methodology

  • The researchers conducted studies on the muscles of different animals that were made to run on a treadmill at their maximum oxygen consumption rate (VO2,max).
  • The process of labeling blood flow to individual muscles was done through injecting intravascular radioactive microspheres.
  • The muscles were then removed and preserved using standard methods for electron microscopy for later analysis.
  • Additionally, the researchers measured the volume density of mitochondria within these muscles to gauge their oxidative capacity.

Data Collection and Analysis

  • The data collection involved measuring the capillary densities in the muscle cross-sections, capillary diameters, and capillary tortuosities (curves).
  • These measurements were used to estimate the capillary volume per unit muscle mass.
  • The mean capillary transit time (the average time it takes for blood to pass through the capillaries) was then estimated by dividing mass-specific capillary volume by mass-specific blood flow.
  • These estimates varied across the different animals and muscles studied, with times ranging from approximately 1 second in the heart and thigh muscles of a horse, to 0.2 seconds in the gastrocnemius (calf muscle) of a duck.

Findings and Insights

  • The collected data showed a hyperbolic relationship between muscle blood flow and capillary transit time. In simpler terms, as blood flow increased, the transit time would decrease, but at a diminishing rate.
  • According to the data, the limit for transit times at extremely high blood flows was found to be 0.2 seconds.
  • Interestingly, the researchers did not find a correlation between capillary transit time and body-mass-specific maximum oxygen consumption.

Cite This Article

APA
Kayar SR, Hoppeler H, Armstrong RB, Laughlin MH, Lindstedt SL, Jones JH, Conley KR, Taylor CR. (1992). Estimating transit time for capillary blood in selected muscles of exercising animals. Pflugers Arch, 421(6), 578-584. https://doi.org/10.1007/BF00375054

Publication

Pflugers Archiv : European journal of physiology
ISSN: 0031-6768
NlmUniqueID: 0154720
Country: Germany
Language: English
Volume: 421
Issue: 6
Pages: 578-584

Researcher Affiliations

Kayar, S R
  • Department of Anatomy, University of Bern, Switzerland.
Hoppeler, H
    Armstrong, R B
      Laughlin, M H
        Lindstedt, S L
          Jones, J H
            Conley, K R
              Taylor, C R

                MeSH Terms

                • Animals
                • Blood Flow Velocity / physiology
                • Capillaries / anatomy & histology
                • Capillaries / physiology
                • Cattle
                • Ducks
                • Foxes
                • Goats
                • Horses
                • Muscles / blood supply
                • Muscles / physiology
                • Physical Conditioning, Animal
                • Regional Blood Flow
                • Swine
                • Time Factors

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                Citations

                This article has been cited 5 times.
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                3. Johnson DE, Casey JR. Cytosolic H+ microdomain developed around AE1 during AE1-mediated Cl-/HCO3- exchange. J Physiol 2011 Apr 1;589(Pt 7):1551-69.
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