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Home / Equine Research Bank / Cytokine / Evaluation of the anti-inflammatory effects of two platelet-...
Cytokine2015; 76(2); 505-513; doi: 10.1016/j.cyto.2015.07.008

Evaluation of the anti-inflammatory effects of two platelet-rich gel supernatants in an in vitro system of cartilage inflammation.

Abstract: To study, in normal cartilage explants (CEs) challenged with lipopolysaccharide (LPS), the temporal effects (at 48 and 96h) of leukocyte- and platelet-rich gel (L-PRG) and pure platelet-rich gel (P-PRG) supernatants on the production and degradation of platelet-associated growth factors (GFs) (platelet-derived GF isoform BB [PDGF-BB] and transforming growth factor beta-1 [TGF-β1]), pro-inflammatory (tumour necrosis factor alpha [TNF-α]) and anti-inflammatory cytokines (interleukin 4 [IL-4] and IL-1 receptor antagonist [IL-1ra]). Methods: CEs from six horses were challenged with LPS and cultured for 96h with L-PRG and P-PRG supernatants at concentrations of 25% and 50%, respectively. The CE culture medium was changed every 48h and used for determination, by ELISA, of PDGF-BB, TGF-β1, TNF-α, IL-4 and IL-1ra. Results: Both the 25% and 50% PRG supernatants produced a different molecular profile in the culture media, unlike that of the CE challenged with LPS only. 50% L-PRG produced the most sustained release of growth factors and anti-inflammatory cytokines, although it produced the highest TNF-α release. PDGF-BB was significantly correlated with IL-1ra and TNF-α concentrations, whereas TNF-α was correlated with IL-4. Conclusions: 50% L-PRG supernatant produced a more sustained concentration of growth factors and anti-inflammatory cytokines than the other hemoderivatives evaluated. This substance could be evaluated in animal models of arthritis or in patients with arthropathies.
Copyright © 2015 Elsevier Ltd. All rights reserved.
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Publication Date: 2015-07-13 PubMed ID: 26185893DOI: 10.1016/j.cyto.2015.07.008Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aims to understand the anti-inflammatory effects of two types of platelet-rich gel supernatants, L-PRG and P-PRG. These effects were studied in an in vitro cartilage inflammation system, by comparing their effects on the production and degradation of various growth factors and cytokines at different time points. The study suggested that L-PRG supernatants in 50% concentration led to a more sustained release of growth factors and anti-inflammatory cytokines, indicating potential use in arthritis treatment.

Research Methodology

  • The researchers used normal cartilage explants (CEs) from six horses for this study. These explants were challenged with the inflammatory agent LPS (lipopolysaccharide).
  • The CEs were then cultured for 96 hours with two types of platelet-rich gel supernatants – leukocyte and platelet-rich gel (L-PRG), and pure platelet-rich gel (P-PRG), at concentrations of 25% and 50% respectively.
  • The CE culture medium was replaced every 48 hours and used further to establish the levels of platelet-derived growth factor isoform BB (PDGF-BB), transforming growth factor beta-1 (TGF-β1), tumor necrosis factor alpha (TNF-α), interleukin 4 (IL-4), and IL-1 receptor antagonist (IL-1ra) using ELISA tests.

Research Findings

  • Both concentrations (25% and 50%) of L-PRG and P-GRG supernatants produced a different molecular profile in the culture medium than that of the CE challenged only with LPS.
  • The 50% L-PRG supernatant led to the most sustained release of growth factors and anti-inflammatory cytokines. However, it also caused the highest release of the pro-inflammatory cytokine, TNF-α.
  • The study also found significant correlations between different growth factors and cytokines. There was a significant correlation between PDGF-BB and both IL-1ra and TNF-α, while TNF-α was correlated with IL-4.

Conclusion and Potential Applications

  • The results of the study indicated that the 50% L-PRG supernatant provided a more sustained concentration of growth factors and anti-inflammatory cytokines compared to other hemoderivatives evaluated in the study.
  • This suggests that it may be beneficial in controlling inflammation and promoting healing, which makes it worthy of evaluation in animal models of arthritis or potentially in human patients suffering from arthropathies.

Cite This Article

APA
Ríos DL, López C, Carmona JU. (2015). Evaluation of the anti-inflammatory effects of two platelet-rich gel supernatants in an in vitro system of cartilage inflammation. Cytokine, 76(2), 505-513. https://doi.org/10.1016/j.cyto.2015.07.008

Publication

Cytokine
ISSN: 1096-0023
NlmUniqueID: 9005353
Country: England
Language: English
Volume: 76
Issue: 2
Pages: 505-513
PII: S1043-4666(15)30018-1

Researcher Affiliations

Ríos, D L
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No 26-10, Manizales, Colombia.
López, C
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No 26-10, Manizales, Colombia.
Carmona, J U
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Calle 65 No 26-10, Manizales, Colombia. Electronic address: carmona@ucaldas.edu.co.

MeSH Terms

  • Animals
  • Arthritis / prevention & control
  • Blood Platelets
  • Cartilage, Articular / drug effects
  • Culture Media
  • Cytokines / biosynthesis
  • Cytokines / metabolism
  • Enzyme-Linked Immunosorbent Assay
  • Gels
  • Horses
  • In Vitro Techniques
  • Lipopolysaccharides / pharmacology

Citations

This article has been cited 12 times.
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  2. Gallego M, López C, Carmona JU. Evaluation of the Pro-, Anti-Inflammatory, and Anabolic Effects of Autologous Platelet-Rich Gel Supernatants in an in vitro Coculture System of Canine Osteoarthritis.. Vet Med Int 2022;2022:3377680.
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    doi: 10.3389/fvets.2021.641818pubmed: 33869321google scholar: lookup
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    doi: 10.1186/s13028-019-0460-6pubmed: 31146775google scholar: lookup
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    doi: 10.1155/2017/6059485pubmed: 28761774google scholar: lookup
  10. Casabona F, Gambelli I, Casabona F, Santi P, Santori G, Baldelli I. Autologous platelet-rich plasma (PRP) in chronic penile lichen sclerosus: the impact on tissue repair and patient quality of life.. Int Urol Nephrol 2017 Apr;49(4):573-580.
    doi: 10.1007/s11255-017-1523-0pubmed: 28161837google scholar: lookup
  11. Carmona JU, Ríos DL, López C, Álvarez ME, Pérez JE, Bohórquez ME. In vitro effects of platelet-rich gel supernatants on histology and chondrocyte apoptosis scores, hyaluronan release and gene expression of equine cartilage explants challenged with lipopolysaccharide.. BMC Vet Res 2016 Jul 1;12(1):135.
    doi: 10.1186/s12917-016-0759-8pubmed: 27369779google scholar: lookup
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    doi: 10.1371/journal.pone.0156137pubmed: 27258008google scholar: lookup
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