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BMC physiology2009; 9; 12; doi: 10.1186/1472-6793-9-12

Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses.

Abstract: The stress response is a critical factor in the training of equine athletes; it is important for performance and for protection of the animal against physio-pathological disorders.In this study, the molecular mechanisms involved in the response to acute and strenuous exercise were investigated using peripheral blood mononuclear cells (PBMCs). Results: Quantitative real-time PCR (qRT-PCR) was used to detect modifications in transcription levels of the genes for matrix metalloproteinase-1 (MMP-1) and interleukin 8 (IL-8), which were derived from previous genome-wide expression analysis. Significant up-regulation of these two genes was found in 10 horses that had completed a race of 90-120 km in a time-course experimental design. Conclusions: These results suggest that MMP-1 and IL-8 are both involved in the exercise-induced stress response, and this represents a starting point from which to understand the adaptive responses to this phenomenon.
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Publication Date: 2009-06-24 PubMed ID: 19552796PubMed Central: PMC2705340DOI: 10.1186/1472-6793-9-12Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the molecular mechanisms in horses that respond to intense exercise, focusing on changes in the genes MMP-1 and IL-8. The findings suggest these genes play a role in the stress response to exercise.

Understanding the Study Objectives

  • This study centers around understanding the effects of rigorous exercise on horses, particularly the molecular mechanisms that spring into action as a response.
  • The focus lies on two genes – matrix metalloproteinase-1 (MMP-1) and Interleukin 8 (IL-8) – derived from previous genome-wide expression analysis. These genes were chosen because changes in their transcription levels were noted under exercise stress.

Methodology of the Research

  • The research involved a group of 10 horses that had completed a race of distance between 90 to 120 km.
  • The researchers collected peripheral blood mononuclear cells (PBMCs) from the horses as the primary tool for investigation. These cells allow researchers to analyze their genetic responses to specific conditions, in this case, strenuous exercise.
  • A Quantitative real-time PCR (qRT-PCR), a laboratory technique used to amplify and simultaneously quantify a targeted DNA molecule, was used to identify modifications in the transcription levels of MMP-1 and IL-8.

Key Findings of the Research

  • The research found a significant increase/up-regulation in the transcription levels of both MMP-1 and IL-8 genes in the horses following strenuous exercise.
  • These genetically regulated responses suggest a biological reaction to the stress induced by exercise. Essentially, it shows that these genes likely play a central role in how a horse’s body responds to the stress of exercise.

Conclusions of the Study

  • The research concludes that both MMP-1 and IL-8 genes are seemingly involved in the equine exercise-induced stress response.
  • This finding provides a foundation for future exploration and understanding of how horses physiologically adapt to rigorous exercise; forming the basis for better training methods and potential protection against disorders related to intense exercise.

Cite This Article

APA
Cappelli K, Felicetti M, Capomaccio S, Pieramati C, Silvestrelli M, Verini-Supplizi A. (2009). Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses. BMC Physiol, 9, 12. https://doi.org/10.1186/1472-6793-9-12

Publication

BMC physiology
ISSN: 1472-6793
NlmUniqueID: 101088687
Country: England
Language: English
Volume: 9
Pages: 12

Researcher Affiliations

Cappelli, Katia
  • Pathology, Diagnostic and Veterinary Clinic Department, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy. katia.cappelli@unipg.it
Felicetti, Michela
    Capomaccio, Stefano
      Pieramati, Camillo
        Silvestrelli, Maurizio
          Verini-Supplizi, Andrea

            MeSH Terms

            • Animals
            • Horses / physiology
            • Interleukin-8 / physiology
            • Matrix Metalloproteinase 1 / physiology
            • Physical Conditioning, Animal / methods
            • Physical Endurance / physiology
            • Stress, Physiological / physiology
            • Up-Regulation / physiology

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            Citations

            This article has been cited 8 times.
            1. Valigura HC, Leatherwood JL, Martinez RE, Norton SA, White-Springer SH. Dietary supplementation of a Saccharomyces cerevisiae fermentation product attenuates exercise-induced stress markers in young horses. J Anim Sci 2021 Aug 1;99(8).
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