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Journal of clinical microbiology1985; 22(2); 265-269; doi: 10.1128/jcm.22.2.265-269.1985

Experimental reproduction of Potomac horse fever in horses with a newly isolated Ehrlichia organism.

Abstract: Potomac horse fever, a recently recognized disease of equines, characterized by high fever, leukopenia, and a profuse diarrhea, was studied for its etiology. An Ehrlichia organism was isolated in equine macrophage-fibroblast cell cultures and mouse macrophage cell cultures from the mononuclear cells of blood of infected horses. The agent was continuously propagated in mouse macrophage cell cultures. The organism multiplied in the cytoplasm of mouse macrophage cells and was identified by Giemsa staining, acridine orange staining, and by indirect immunofluorescence with convalescent sera from infected horses. The disease was experimentally reproduced in horses inoculated with Ehrlichia-infected cell culture material. The Ehrlichia organism was reisolated from the blood of these infected horses during the course of the disease. Antibody against the organism was detected in the sera of experimentally infected horses. This study confirmed that the new Ehrlichia organism is the etiological agent of Potomac horse fever.
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Publication Date: 1985-08-01 PubMed ID: 4031040PubMed Central: PMC268372DOI: 10.1128/jcm.22.2.265-269.1985Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the cause of Potomac horse fever, known for high fever and diarrhoea in horses, and determines that the newly isolated Ehrlichia organism is responsible for the disease.

Introduction

The study was aimed at exploring the etiology of Potomac horse fever, a common ailment affecting horses, which was isolated in equine and mouse macrophage cell cultures sourced from the blood of infected horses.

Methodology

  • The researchers used a newly isolated Ehrlichia organism that was propagated in mouse macrophage cell cultures for their experimentation.
  • This Ehrlichia organism developed in the cytoplasm of mouse macrophage cells and was identified with Giemsa staining, acridine orange staining and indirect immunofluorescence using convalescent sera from infected horses for verification.

Experiment and Results

  • To experimentally reproduce the disease, horses were injected with the Ehrlichia-infected cell culture material.
  • The Ehrlichia microorganism was then reobtained from the blood of these infected horses during the disease’s progression.
  • The researchers were successful in detecting an antibody against the organism in the serum taken from experimentally infected horses.

Conclusion

This research affirms that the newly discovered Ehrlichia organism is the etiological agent responsible for causing Potomac horse fever. This discovery represents a significant step forward in understanding the disease, potentially leading to improved treatments and prevention strategies.

Cite This Article

APA
Dutta SK, Myrup AC, Rice RM, Robl MG, Hammond RC. (1985). Experimental reproduction of Potomac horse fever in horses with a newly isolated Ehrlichia organism. J Clin Microbiol, 22(2), 265-269. https://doi.org/10.1128/jcm.22.2.265-269.1985

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 22
Issue: 2
Pages: 265-269

Researcher Affiliations

Dutta, S K
    Myrup, A C
      Rice, R M
        Robl, M G
          Hammond, R C

            MeSH Terms

            • Animals
            • Antibodies, Bacterial / isolation & purification
            • Cells, Cultured
            • Disease Models, Animal
            • Ehrlichia / immunology
            • Ehrlichia / isolation & purification
            • Horse Diseases / etiology
            • Horse Diseases / microbiology
            • Horses
            • Mice
            • Rickettsiaceae / isolation & purification
            • Rickettsiaceae Infections / etiology
            • Rickettsiaceae Infections / microbiology
            • Rickettsiaceae Infections / veterinary

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            Citations

            This article has been cited 25 times.
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