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Expression of leukaemia inhibitory factor at the conceptus?maternal interface during preimplantation development and in the endometrium during the oestrous cycle in the mare.
Abstract: Leukaemia inhibitory factor (LIF) plays a critical role in blastocyst development and implantation in several species. The present study investigated mRNA and protein expression for LIF, as well as the low-affinity LIF receptor (LIFR) and interleukin-6 signal transducer (IL6ST), in equine endometrium, trophoblast and histotroph during early pregnancy and in the endometrium during the oestrous cycle. Endometrial LIF mRNA expression was upregulated after Day 21 of pregnancy, whereas LIF immunoreactivity increased in the endometrium on Day 28. Expression of LIF mRNA in the yolk sac membrane increased from Day 21 of pregnancy, whereas LIF immunoreactivity increased from Day 28 in the trophoblast. LIFR and IL6ST mRNA was expressed in the endometrium during both the oestrous cycle and early pregnancy and, although LIFR and IL6ST protein were localised to the glandular epithelium during the cycle and first 14 days of pregnancy, from Day 21 they were located in the luminal epithelium. Trophoblast expression of LIFR and IL6ST increased as pregnancy proceeded. In conclusion, LIF expression increased at the conceptus-maternal interface during capsule attenuation. Because contemporaneous upregulation of both LIFR and IL6ST was also observed in the trophoblast, we propose that LIF plays an important role in the development of endometrial receptivity for trophoblast growth, apposition and adhesion in mares.
Publication Date: 2015-04-17 PubMed ID: 25881292DOI: 10.1071/RD14334Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research article focuses on examining the role of the leukaemia inhibitory factor (LIF) in blastocyst development and implantation in horses. The study particularly looks into the expression of LIF, LIF receptor (LIFR) and interleukin-6 signal transducer (IL6ST) during early pregnancy and the oestrous cycle, and suggests that LIF plays a vital role in the development of endometrial receptivity for trophoblast growth, apposition and adhesion in horses.
Expression of Leukaemia Inhibitory Factor (LIF) during preimplantation development
- The study found that the expression of LIF mRNA was upregulated in the equine endometrium after the 21st day of pregnancy.
- LIF immunoreactivity, or the ability of the immune system to respond to LIF, increased in the endometrium on the 28th day.
- Additionally, the researchers observed an increase in the expression of LIF mRNA in the yolk sac membrane from the 21st day of pregnancy. The LIF immunoreactivity increased from the 28th day in the trophoblast, a part of the mammalian blastocyst involved in implantation.
Expression of LIF receptor (LIFR) and interleukin-6 signal transducer (IL6ST) during the oestrous cycle
- Endometrium during both the oestrous cycle and early pregnancy expressed LIFR and IL6ST mRNA.
- During the cycle and the initial 14 days of pregnancy, LIFR and IL6ST proteins were localized to the glandular epithelium. However, from the 21st day of pregnancy, they were found in the luminal epithelium.
- The trophoblast’s expression of both LIFR and IL6ST increased as the pregnancy progressed.
Conclusion
- Conclusively, the researchers found an increase in LIF expression at the junction between the conceptus and mother during capsule attenuation, a phase in preimplantation development.
- The simultaneous increase of LIFR and IL6ST in the trophoblast suggests that LIF plays a critical role in fostering endometrial receptivity. It aids with trophoblast growth, its apposition or proximity with maternal tissues, and adhesion in horses.
Cite This Article
APA
de Ruijter-Villani M, Deelen C, Stout TA.
(2015).
Expression of leukaemia inhibitory factor at the conceptus?maternal interface during preimplantation development and in the endometrium during the oestrous cycle in the mare.
Reprod Fertil Dev.
https://doi.org/10.1071/RD14334 Publication
Researcher Affiliations
Citations
This article has been cited 5 times.- Gibson C, de Ruijter-Villani M, Stout TAE. Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy. Front Vet Sci 2022;9:912721.
- Juárez-Barber E, Francés-Herrero E, Corachán A, Vidal C, Giles J, Alamá P, Faus A, Pellicer A, Cervelló I, Ferrero H. Establishment of Adenomyosis Organoids as a Preclinical Model to Study Infertility. J Pers Med 2022 Feb 4;12(2).
- Klein C, Bruce P, Hammermueller J, Hayes T, Lillie B, Betteridge K. Transcriptional profiling of equine endometrium before, during and after capsule disintegration during normal pregnancy and after oxytocin-induced luteostasis in non-pregnant mares. PLoS One 2021;16(10):e0257161.
- Eozenou C, Lesage-Padilla A, Mauffré V, Healey GD, Camous S, Bolifraud P, Giraud-Delville C, Vaiman D, Shimizu T, Miyamoto A, Sheldon IM, Constant F, Pannetier M, Sandra O. FOXL2 is a Progesterone Target Gene in the Endometrium of Ruminants. Int J Mol Sci 2020 Feb 21;21(4).
- Bonometti S, Menarim BC, Reinholt BM, Ealy AD, Johnson SE. Growth factor modulation of equine trophoblast mitosis and prostaglandin gene expression. J Anim Sci 2019 Feb 1;97(2):865-873.
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