Expression of wingless type (WNT) genes and their antagonists at mRNA levels in equine endometrium during the estrous cycle and early pregnancy.
Abstract: WNT signaling pathway plays important roles in reproductive events. Aims were to (1) determine presence of WNT genes and their antagonists in equine endometrium; and (2) to evaluate their expression profiles during early pregnancy. Endometrial biopsies were obtained from mares on day of ovulation (d0, n=4) and on days of 14 (P14, n=4), 18 (P18, n=4), 22 (P22, n=4) of early pregnancy. Biopsies were also collected from cyclic mares during late diestrus (LD, on day of 13.5-14, n=4) and after luteolysis in estrus phase (AL, on day of 17.5-18, n=4) of the cycle. PCR was used to detect expression of genes studied and then relative expression levels were quantified using real-time PCR analysis. A mixed model was fitted on the normalized data and least significant difference test (α=0.05) was employed. Eleven WNT genes (WNT2, WNT2B, WNT4, WNT5A, WNT5B, WNT7A, WNT8A, WNT9B, WNT10B, WNT11 and WNT16) and their antagonists (SFRP1, SFRP2, SFRP5, DKK1, DKK2 and WIF-1) were detected in equine endometrium. Compared to d0, WNT2, WNT5B, WNT7A and SFRP1 expressions were downregulated by the pregnancy while DKK1 was upregulated. WNT5A, WNT11 and WIF-1 were upregulated on P14 and P18, but WNT2B increased only on P14. When LD and P14 were compared, level of WNT8A decreased on P14 while increase in WNT4 level on P14 was slightly significant (P<0.06). Levels of WNT7A and SFRP1 decreased while DKK1 and WIF-1 increased by the pregnancy on P18 compared to AL. Moreover, WNT2B, WNT5A, WNT9B, WNT10B, WNT11, WNT16 DKK1 and WIF-1 were upregulated on LD compared to AL whereas WNT4, WNT7A, SFRP1 were downregulated. In conclusion, the results demonstrate that WNT genes and their antagonists appear to be regulated during early pregnancy in equine endometrium possibly due to embryonic factors and/or maternal progesterone.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication Date: 2011-04-14 PubMed ID: 21550190DOI: 10.1016/j.anireprosci.2011.04.001Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research examines the expression of wingless type (WNT) genes and their antagonists in horse endometrium during the estrous cycle and early pregnancy, highlighting some genes are regulated during early gestation by embryonic factors or maternal progesterone.
Aims and Methodology
- The main objectives of the study were:
- To identify the presence of WNT genes and their antagonists in the horse endometrium.
- To study their expression profiles during early pregnancy.
- Endometrial biopsies were taken from mares at specific points throughout the estrous cycle and early stages of pregnancy.
- The detected expression of genes was interrogated using Polymerase Chain Reaction (PCR) and the relative expression levels were quantified using real-time PCR analysis.
- A statistical model was used to interpret the data.
Findings
- Eleven WNT genes and their antagonists were detected in horse endometrium.
- Comparing to the day of ovulation, the expression levels of four WNT genes were downregulated by pregnancy, while a gene known as DKK1 showed an upregulated expression.
- Some WNT genes displayed increased expression on the 14th and 18th day of pregnancy, whereas WNT2B showed elevated expression only on the 14th day.
- When late diestrus and the 14th day of pregnancy were compared, the study observed a decrease in WNT8A levels on the 14th day and a slightly significant increase in WNT4 levels.
- The study also observed changes in gene expression between the 18th day on estrous and normal cycles, highlighting the role of pregnancy in altering WNT gene expressions.
Conclusion
- This study reinforces the role WNT genes and their antagonists play on the horse endometrium during early pregnancy.
- Changes in gene expression suggest they could be regulated by embryonic factors and/or maternal progesterone at the beginning of gestation.
- This research may potentially inform future studies about the role of WNT genes in equine reproduction.
Cite This Article
APA
Atli MO, Guzeloglu A, Dinc DA.
(2011).
Expression of wingless type (WNT) genes and their antagonists at mRNA levels in equine endometrium during the estrous cycle and early pregnancy.
Anim Reprod Sci, 125(1-4), 94-102.
https://doi.org/10.1016/j.anireprosci.2011.04.001 Publication
Researcher Affiliations
- Department of OBGYN, Faculty of Veterinary Medicine, Dicle University, Diyarbakir 21280, Turkey. moatli@hotmail.com
MeSH Terms
- Animals
- Biopsy / veterinary
- Endometrium / metabolism
- Endometrium / physiology
- Estrous Cycle / genetics
- Estrous Cycle / physiology
- Female
- Frizzled Receptors / biosynthesis
- Frizzled Receptors / genetics
- Gene Expression Regulation / physiology
- Horses / genetics
- Horses / metabolism
- Horses / physiology
- Male
- Pregnancy
- RNA, Messenger / biosynthesis
- RNA, Messenger / genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Wnt Proteins / biosynthesis
- Wnt Proteins / genetics
Citations
This article has been cited 12 times.- Aguilera C, Velásquez AE, Gutierrez-Reinoso MA, Wong YS, Melo-Baez B, Cabezas J, Caamaño D, Navarrete F, Rojas D, Riadi G, Castro FO, Rodriguez-Alvarez L. Extracellular Vesicles Secreted by Pre-Hatching Bovine Embryos Produced In Vitro and In Vivo Alter the Expression of IFNtau-Stimulated Genes in Bovine Endometrial Cells.. Int J Mol Sci 2023 Apr 18;24(8).
- Vaishnav S, Chauhan A, Ajay A, Saini BL, Kumar S, Kumar A, Bhushan B, Gaur GK. Allelic to genome wide perspectives of swine genetic variation to litter size and its component traits.. Mol Biol Rep 2023 Apr;50(4):3705-3721.
- Zhang Z, Sui Z, Zhang J, Li Q, Zhang Y, Xing F. Transcriptome Sequencing-Based Mining of Genes Associated With Pubertal Initiation in Dolang Sheep.. Front Genet 2022;13:818810.
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