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Parasitology research2024; 123(9); 314; doi: 10.1007/s00436-024-08330-x

Extracellular vesicles released by Trypanosoma evansi: induction analysis and proteomics.

Abstract: Trypanosoma evansi is a unicellular protozoan responsible for causing a disease known as "surra," which is found in different regions of the world and primarily affects horses and camels. Few information is known about virulence factors released from the parasite within the animals. The organism can secrete extracellular vesicles (EVs), which transport a variety of molecules, including proteins. Before being considered exclusively as a means for eliminating unwanted substances, extracellular vesicles (EVs) have emerged as key players in intercellular communication, facilitating interactions between cells, host cells, and parasites, and even between parasites themselves. Thus, they may be used as potential biomarkers. This study aimed to assess the induction of EVs production by Ca, conduct a proteomic analysis of the EVs released by T. evansi, and identify epitopes that could serve as biomarkers. The findings indicated that Ca is not an effective promoter of vesiculation in T. evansi. Furthermore, the proteomic analysis has identified multiple proteins that have been investigated as biomarkers or vaccine antigens, previously. A total of 442 proteins were identified, with 7 of them specifically recognizing 9 epitopes that are unique to T. evansi. At least one of these epitopes of TevSTIB805.9.11580 have been previously identified, which increases the possibility of further investigating its potential as a biomarker.
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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Publication Date: 2024-09-03 PubMed ID: 39225716PubMed Central: 10975194DOI: 10.1007/s00436-024-08330-xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the release of extracellular vesicles (EVs) by Trypanosoma evansi, a protozoan that causes a disease called “surra”. The main objectives were determining the effect of calcium in producing EVs, identifying proteins within these vesicles and potential unique biomarker epitopes. The study finds that calcium doesn’t effectively induce vesicle creation, but identifies several proteins and epitopes that could be potential biomarkers.

Analysis of Extracellular Vesicle Release Induction

  • The study aimed to understand how calcium (Ca) affected EV release by Trypanosoma evansi. EVs are tiny bubbles secreted from cells that transport various molecules like proteins.
  • These vesicles, earlier thought to help discard unwanted substances, are now known to facilitate intercellular communication. These vesicles assist in interactions between different cells, host cells and parasites, and even between parasites themselves.
  • However, the study found that calcium was not an effective promoter of vesiculation (formation of vesicles) in Trypanosoma evansi.

Proteomic Analysis of EVs

  • The research also included a proteomic analysis of the EVs released by Trypanosoma evansi, aiming to catalogue their protein content.
  • The proteomic analysis is important as these proteins could potentially be investigated as biomarkers or vaccine antigens for surra disease.
  • The analysis identified a total of 442 proteins within the EVs.

Identifying Potential Biomarkers

  • Out of the identified proteins, 7 proteins specifically recognized 9 unique epitopes that are unique to Trypanosoma evansi.
  • Epitopes are parts of an antigen that an immune system’s antibodies latch onto. If an epitope is unique to a certain pathogen, like Trypanosoma evansi, then it could serve as an effective biomarker for that disease.
  • At least one of these epitopes (from the TevSTIB805.9.11580 protein) had been previously identified, thereby increasing the potential for its further investigation as a biomarker for the disease caused by Trypanosoma evansi.

Cite This Article

APA
Ungri AM, Dos Santos Sabatke BF, Rossi IV, das Neves GB, Marques J, Ribeiro BG, Borges GK, Moreira RS, Ramírez MI, Miletti LC. (2024). Extracellular vesicles released by Trypanosoma evansi: induction analysis and proteomics. Parasitol Res, 123(9), 314. https://doi.org/10.1007/s00436-024-08330-x

Publication

Parasitology research
ISSN: 1432-1955
NlmUniqueID: 8703571
Country: Germany
Language: English
Volume: 123
Issue: 9
Pages: 314

Researcher Affiliations

Ungri, Amanda Martins
  • Laboratório de Hemoparasitas E Vetores, Centro de Ciências Agroveterinárias (CAV), Universidade Do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Conta Dinheiro, Lages, 88520-000, SC, Brazil.
Dos Santos Sabatke, Bruna Fernanda
  • Laboratório de Biologia Celular, EVAHPI-Extracellular Vesicles and Host-Parasite Interactions Research Group, Instituto Carlos Chagas-Fiocruz, Curitiba, 81310-020, Brazil.
Rossi, Izadora Volpato
  • Laboratório de Biologia Celular, EVAHPI-Extracellular Vesicles and Host-Parasite Interactions Research Group, Instituto Carlos Chagas-Fiocruz, Curitiba, 81310-020, Brazil.
das Neves, Gabriella Bassi
  • Laboratório de Hemoparasitas E Vetores, Centro de Ciências Agroveterinárias (CAV), Universidade Do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Conta Dinheiro, Lages, 88520-000, SC, Brazil.
Marques, Júlia
  • Laboratório de Hemoparasitas E Vetores, Centro de Ciências Agroveterinárias (CAV), Universidade Do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Conta Dinheiro, Lages, 88520-000, SC, Brazil.
Ribeiro, Brenda Guedes
  • Laboratório de Hemoparasitas E Vetores, Centro de Ciências Agroveterinárias (CAV), Universidade Do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Conta Dinheiro, Lages, 88520-000, SC, Brazil.
Borges, Gabriela Kaiser
  • Laboratório de Hemoparasitas E Vetores, Centro de Ciências Agroveterinárias (CAV), Universidade Do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Conta Dinheiro, Lages, 88520-000, SC, Brazil.
Moreira, Renato Simões
  • Instituto Federal de Santa Catarina (IFSC), Campus Gaspar, R. Adriano Kormann, 510-Bela Vista, Gaspar, SC, Brazil.
Ramírez, Marcel Ivan
  • Laboratório de Biologia Celular, EVAHPI-Extracellular Vesicles and Host-Parasite Interactions Research Group, Instituto Carlos Chagas-Fiocruz, Curitiba, 81310-020, Brazil.
Miletti, Luiz Claudio
  • Laboratório de Hemoparasitas E Vetores, Centro de Ciências Agroveterinárias (CAV), Universidade Do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Conta Dinheiro, Lages, 88520-000, SC, Brazil. luiz.miletti@udesc.br.
  • Departamento de Produção Animal E Alimentos, Centro de Ciências Agroveterinárias, Universidade Do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Bairro Conta Dinheiro, Lages, SC, 88520-000, Brazil. luiz.miletti@udesc.br.

MeSH Terms

  • Trypanosoma / metabolism
  • Trypanosoma / genetics
  • Extracellular Vesicles / metabolism
  • Proteomics
  • Protozoan Proteins / genetics
  • Protozoan Proteins / metabolism
  • Animals
  • Calcium / metabolism
  • Biomarkers
  • Trypanosomiasis / parasitology
  • Proteome
  • Epitopes / immunology

Grant Funding

  • 402165/ 2016-4 / Conselho Nacional de Desenvolvimento Cientu00edfico e Tecnolu00f3gico
  • 2019TR655 / Fundau00e7u00e3o de Amparo u00e0 Pesquisa e Inovau00e7u00e3o do Estado de Santa Catarina

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Citations

This article has been cited 1 times.
  1. Rodrigues A, Weber JI, Durães-Oliveira J, Moreno C, Ferla M, Aires Pereira M, Valério-Bolas A, Freitas BE, Nunes T, Antunes WT, Alexandre-Pires G, Pereira da Fonseca I, Santos-Gomes GM. Extracellular Vesicles Derived from Trypanosomatids: The Key to Decoding Host-Parasite Communication. Int J Mol Sci 2025 May 1;26(9).
    doi: 10.3390/ijms26094302pubmed: 40362539google scholar: lookup
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