FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Osteoarthritis and cartilage2016; 25(3); 369-375; doi: 10.1016/j.joca.2016.09.005

Factors that influence outcome in experimental osteoarthritis.

Abstract: Osteoarthritis (OA) is the most common joint disease but an effective pharmacological therapy has not been developed yet. To identify targets for treatment and ways to interfere with OA development and progression both spontaneous and induced OA models are still needed. In this narrative review it is discussed what variables can be identified that lead to variation in OA animal model studies. Literature was screened (Pubmed) with the following terms; OA animal models in combination with species, age, strain, gender/sex, housing, diet, fighting, circadian rhythm, transgenic. Relevant articles were selected and additional papers were searched for and read for specific subtopics. Studies with OA models are subject to a multitude of variables, stimuli and conditions that can influence the outcome of an animal experiment. Outcome will depend on amongst others; the model used, species and strain, age, gender, diet, housing conditions, circadian rhythm, timing of intervention, stress levels and activity. Variations in these variables can account for discrepancies between OA model experiments, intervention studies and conclusions. To improve OA animal model research, investigators should be aware of all the stimuli and conditions that can interfere with disease development and disease intervention and take these into account in their study design and execution.
Copyright © 2016 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
Get Full Text
Publication Date: 2016-09-09 PubMed ID: 27616682DOI: 10.1016/j.joca.2016.09.005Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Review
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the different factors that could potentially influence the results in experimental osteoarthritis (OA) studies conducted on animal models.

Research Methodology

This paper is a narrative review based on a thorough analysis of existing literature, specifically focusing on animal models used in osteoarthritis studies. The researchers conducted a systematic search of articles on Pubmed using specific terms such as species, age, strain, gender/sex, housing, diet, circadian rhythm, and transgenic in combination with OA animal models. From this search, they selected relevant articles and further identified additional papers related to subtopics of interest for detailed examination.

Variables Influencing Osteoarthritis Models

The study outlines that results from osteoarthritis models can be subject to a range of variables, stimuli, and conditions which can significantly influence the outcomes of the experiments. These include:

  • Model used: The specific type of osteoarthritis model utilized in the study can significantly influence the outcome.
  • Species and strain: Variations in different species and strains used can lead to discrepancies in results.
  • Age and gender: The age and gender of the animal models can greatly impact the development and progression of OA, thus affecting the study outcomes.
  • Diet and housing conditions: These environmental variables can affect disease progression and the effectiveness of interventions.
  • Circadian rhythms, stress levels, and activity: These variables related to the biology and behavior of the animals can complicate the measurements and outcomes of the OA studies.

Implications for Future Research

Given the array of factors that can potentially influence the results of OA studies, the authors suggest that future researchers need to be aware of these stimuli and conditions that may interfere with the development of the disease and the effectiveness of interventions. By carefully considering and controlling these variables in their experimental design and execution, researchers can improve the accuracy and reliability of their results, making them more conducive to developing effective therapeutic strategies for osteoarthritis.

Cite This Article

APA
van der Kraan PM. (2016). Factors that influence outcome in experimental osteoarthritis. Osteoarthritis Cartilage, 25(3), 369-375. https://doi.org/10.1016/j.joca.2016.09.005

Publication

Osteoarthritis and cartilage
ISSN: 1522-9653
NlmUniqueID: 9305697
Country: England
Language: English
Volume: 25
Issue: 3
Pages: 369-375
PII: S1063-4584(16)30272-2

Researcher Affiliations

van der Kraan, P M
  • Experimental Rheumatology, Radboudumc, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. Electronic address: peter.vanderkraan@radboudumc.nl.

MeSH Terms

  • Age Factors
  • Animals
  • Disease Models, Animal
  • Dogs
  • Female
  • Goats
  • Guinea Pigs
  • Horses
  • Housing, Animal
  • Humans
  • Joints / anatomy & histology
  • Male
  • Mice
  • Mice, Transgenic
  • Osteoarthritis / drug therapy
  • Rabbits
  • Rats
  • Sex Factors
  • Sheep
  • Species Specificity
  • Treatment Outcome

Citations

This article has been cited 32 times.
  1. Lin YY, Kuan CY, Chang CT, Chuang MH, Syu WS, Zhang KL, Lee CH, Lin PC, Dong GC, Lin FH. 3D-Cultured Adipose-Derived Stem Cell Spheres Using Calcium-Alginate Scaffolds for Osteoarthritis Treatment in a Mono-Iodoacetate-Induced Rat Model. Int J Mol Sci 2023 Apr 11;24(8).
    doi: 10.3390/ijms24087062pubmed: 37108239google scholar: lookup
  2. Hart DA, Werle J, Robert J, Kania-Richmond A. Long wait times for knee and hip total joint replacement in Canada: An isolated health system problem, or a symptom of a larger problem?. Osteoarthr Cartil Open 2021 Jun;3(2):100141.
    doi: 10.1016/j.ocarto.2021.100141pubmed: 36474990google scholar: lookup
  3. Sohn HS, Choi JW, Jhun J, Kwon SP, Jung M, Yong S, Na HS, Kim JH, Cho ML, Kim BS. Tolerogenic nanoparticles induce type II collagen-specific regulatory T cells and ameliorate osteoarthritis. Sci Adv 2022 Nov 25;8(47):eabo5284.
    doi: 10.1126/sciadv.abo5284pubmed: 36427299google scholar: lookup
  4. Liu Z, Zhuang Y, Fang L, Yuan C, Wang X, Lin K. Breakthrough of extracellular vesicles in pathogenesis, diagnosis and treatment of osteoarthritis. Bioact Mater 2023 Apr;22:423-452.
    doi: 10.1016/j.bioactmat.2022.10.012pubmed: 36311050google scholar: lookup
  5. Hu W, Lin J, Wei J, Yang Y, Fu K, Zhu T, Zhu H, Zheng X. Modelling osteoarthritis in mice via surgical destabilization of the medial meniscus with or without a stereomicroscope. Bone Joint Res 2022 Aug;11(8):518-527.
    doi: 10.1302/2046-3758.118.BJR-2021-0575.R1pubmed: 35909337google scholar: lookup
  6. Delbaldo C, Tschon M, Martini L, Fini M, Codispoti G. Benefits of Applying Nanotechnologies to Hydrogels in Efficacy Tests in Osteoarthritis Models-A Systematic Review of Preclinical Studies. Int J Mol Sci 2022 Jul 26;23(15).
    doi: 10.3390/ijms23158236pubmed: 35897805google scholar: lookup
  7. Drevet S, Favier B, Brun E, Gavazzi G, Lardy B. Mouse Models of Osteoarthritis: A Summary of Models and Outcomes Assessment. Comp Med 2022 Feb 1;72(1):3-13.
    doi: 10.30802/AALAS-CM-21-000043pubmed: 34986927google scholar: lookup
  8. Iijima H, Gilmer G, Wang K, Sivakumar S, Evans C, Matsui Y, Ambrosio F. Meta-analysis Integrated With Multi-omics Data Analysis to Elucidate Pathogenic Mechanisms of Age-Related Knee Osteoarthritis in Mice. J Gerontol A Biol Sci Med Sci 2022 Jul 5;77(7):1321-1334.
    doi: 10.1093/gerona/glab386pubmed: 34979545google scholar: lookup
  9. Takahashi I, Matsuzaki T, Kuroki H, Hoso M. Physiological Reloading Recovers Histologically Disuse Atrophy of the Articular Cartilage and Bone by Hindlimb Suspension in Rat Knee Joint. Cartilage 2021 Dec;13(2_suppl):1530S-1539S.
    doi: 10.1177/19476035211063857pubmed: 34886706google scholar: lookup
  10. Estrada McDermott J, Pezzanite L, Goodrich L, Santangelo K, Chow L, Dow S, Wheat W. Role of Innate Immunity in Initiation and Progression of Osteoarthritis, with Emphasis on Horses. Animals (Basel) 2021 Nov 13;11(11).
    doi: 10.3390/ani11113247pubmed: 34827979google scholar: lookup
  11. Longo UG, Forriol F, Candela V, Tecce SM, De Salvatore S, Altonaga JR, Wallace AL, Denaro V. Arthroscopic Tenotomy of the Long Head of the Biceps Tendon and Section of the Anterior Joint Capsule Produce Moderate Osteoarthritic Changes in an Experimental Sheep Model. Int J Environ Res Public Health 2021 Jul 13;18(14).
    doi: 10.3390/ijerph18147471pubmed: 34299937google scholar: lookup
  12. Takahashi I, Takeda K, Matsuzaki T, Kuroki H, Hoso M. Reduction of knee joint load suppresses cartilage degeneration, osteophyte formation, and synovitis in early-stage osteoarthritis using a post-traumatic rat model. PLoS One 2021;16(7):e0254383.
    doi: 10.1371/journal.pone.0254383pubmed: 34270585google scholar: lookup
  13. Duan X, Cai L, Pham CTN, Abu-Amer Y, Pan H, Brophy RH, Wickline SA, Rai MF. Amelioration of Posttraumatic Osteoarthritis in Mice Using Intraarticular Silencing of Periostin via Nanoparticle-Based Small Interfering RNA. Arthritis Rheumatol 2021 Dec;73(12):2249-2260.
    doi: 10.1002/art.41794pubmed: 33982891google scholar: lookup
  14. Wang Y, Wu C, Tao J, Zhao D, Jiang X, Tian W. Differential proteomic analysis of tibial subchondral bone from male and female guinea pigs with spontaneous osteoarthritis. Exp Ther Med 2021 Jun;21(6):633.
    doi: 10.3892/etm.2021.10065pubmed: 33968164google scholar: lookup
  15. Kosinska MK, Eichner G, Schmitz G, Liebisch G, Steinmeyer J. A comparative study on the lipidome of normal knee synovial fluid from humans and horses. PLoS One 2021;16(4):e0250146.
    doi: 10.1371/journal.pone.0250146pubmed: 33861772google scholar: lookup
  16. Landi M, Everitt J, Berridge B. Bioethical, Reproducibility, and Translational Challenges of Animal Models. ILAR J 2021 Dec 31;62(1-2):60-65.
    doi: 10.1093/ilar/ilaa027pubmed: 33693624google scholar: lookup
  17. Contartese D, Tschon M, De Mattei M, Fini M. Sex Specific Determinants in Osteoarthritis: A Systematic Review of Preclinical Studies. Int J Mol Sci 2020 May 24;21(10).
    doi: 10.3390/ijms21103696pubmed: 32456298google scholar: lookup
  18. Pinamont WJ, Yoshioka NK, Young GM, Karuppagounder V, Carlson EL, Ahmad A, Elbarbary R, Kamal F. Standardized Histomorphometric Evaluation of Osteoarthritis in a Surgical Mouse Model. J Vis Exp 2020 May 6;(159).
    doi: 10.3791/60991pubmed: 32449702google scholar: lookup
  19. Casper-Taylor ME, Barr AJ, Williams S, Wilcox RK, Conaghan PG. Initiating factors for the onset of OA: A systematic review of animal bone and cartilage pathology in OA. J Orthop Res 2020 Aug;38(8):1810-1818.
    doi: 10.1002/jor.24605pubmed: 31975435google scholar: lookup
  20. Malfait AM, Miller RE. Why we should study osteoarthritis pain in experimental models in both sexes. Osteoarthritis Cartilage 2020 Apr;28(4):397-399.
    doi: 10.1016/j.joca.2019.12.008pubmed: 31926266google scholar: lookup
  21. Brown SB, Hornyak JA, Jungels RR, Shah YY, Yarmola EG, Allen KD, Sharma B. Characterization of Post-Traumatic Osteoarthritis in Rats Following Anterior Cruciate Ligament Rupture by Non-Invasive Knee Injury (NIKI). J Orthop Res 2020 Feb;38(2):356-367.
    doi: 10.1002/jor.24470pubmed: 31520482google scholar: lookup
  22. Allen KD, Chan KM, Yarmola EG, Shah YY, Partain BD. The effects of age on the severity of joint damage and intra-articular inflammation following a simulated medial meniscus injury in 3, 6, and 9 month old male rats. Connect Tissue Res 2020 Jan;61(1):82-94.
    doi: 10.1080/03008207.2019.1641495pubmed: 31438735google scholar: lookup
  23. Brown S, Kumar S, Sharma B. Intra-articular targeting of nanomaterials for the treatment of osteoarthritis. Acta Biomater 2019 Jul 15;93:239-257.
    doi: 10.1016/j.actbio.2019.03.010pubmed: 30862551google scholar: lookup
  24. Son YO, Park S, Kwak JS, Won Y, Choi WS, Rhee J, Chun CH, Ryu JH, Kim DK, Choi HS, Chun JS. Estrogen-related receptor γ causes osteoarthritis by upregulating extracellular matrix-degrading enzymes. Nat Commun 2017 Dec 15;8(1):2133.
    doi: 10.1038/s41467-017-01868-8pubmed: 29247173google scholar: lookup
  25. Xue HY, Shen XL, Wang ZH, Bi HC, Xu HG, Wu J, Cui RM, Liu MW. Research progress on mesenchymal stem cell‑derived exosomes in the treatment of osteoporosis induced by knee osteoarthritis (Review). Int J Mol Med 2025 Oct;56(4).
    doi: 10.3892/ijmm.2025.5601pubmed: 40747674google scholar: lookup
  26. Wu KC, Chang YH, Ding DC, Lin SZ. Mesenchymal Stromal Cells for Aging Cartilage Regeneration: A Review. Int J Mol Sci 2024 Nov 30;25(23).
    doi: 10.3390/ijms252312911pubmed: 39684619google scholar: lookup
  27. Liang W, Feng R, Li X, Duan X, Feng S, Chen J, Li Y, Chen J, Liu Z, Wang X, Ruan G, Tang S, Ding C, Huang B, Zou Z, Chen T. A RANKL-UCHL1-sCD13 negative feedback loop limits osteoclastogenesis in subchondral bone to prevent osteoarthritis progression. Nat Commun 2024 Oct 10;15(1):8792.
    doi: 10.1038/s41467-024-53119-2pubmed: 39389988google scholar: lookup
  28. Oestreich AK, Harasymowicz NS, Savadipour A, Harissa Z, Rashidi N, Luhmann MK, Kuziez M, Moley KH, Guilak F. Maternal high-fat high-sugar diet impairs bone quality and strength but not cartilage in aging mice. J Orthop Res 2025 Jan;43(1):117-127.
    doi: 10.1002/jor.25980pubmed: 39342461google scholar: lookup
  29. Whittaker JL, Kalsoum R, Bilzon J, Conaghan PG, Crossley K, Dodge GR, Getgood A, Li X, Losina E, Mason DJ, Pietrosimone B, Risberg MA, Roemer F, Felson D, Culvenor AG, Meuffels D, Gerwin N, Simon LS, Lohmander LS, Englund M, Watt FE. Toward designing human intervention studies to prevent osteoarthritis after knee injury: A report from an interdisciplinary OARSI 2023 workshop. Osteoarthr Cartil Open 2024 Jun;6(2):100449.
    doi: 10.1016/j.ocarto.2024.100449pubmed: 38440780google scholar: lookup
  30. Hildebrandt A, Dietrich T, Weber J, Günderoth MM, Zhou S, Fleckenstein FN, Jiang S, Winkler T, Duda GN, Tsitsilonis S, Keller J, Maleitzke T. The dual pro-inflammatory and bone-protective role of calcitonin gene-related peptide alpha in age-related osteoarthritis. Arthritis Res Ther 2023 Dec 15;25(1):244.
    doi: 10.1186/s13075-023-03215-3pubmed: 38102666google scholar: lookup
  31. Takahashi I, Matsuzaki T, Kuroki H, Hoso M. Treadmill Exercise Suppresses Histological Progression of Disuse Atrophy in Articular Cartilage in Rat Knee Joints during Hindlimb Suspension. Cartilage 2023 Dec;14(4):482-491.
    doi: 10.1177/19476035231154510pubmed: 36802945google scholar: lookup
  32. Chan KM, Bowe MT, Allen KD. Recommendations for the analysis of rodent gait data to evaluate osteoarthritis treatments. Osteoarthritis Cartilage 2023 Apr;31(4):425-434.
    doi: 10.1016/j.joca.2022.11.006pubmed: 36435413google scholar: lookup
Subscribe to our newsletter
Join 100,127 horse owners like you who receive our email updates on horse health and nutrition.