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Fibronectin mRNA splice variant in articular cartilage lacks bases encoding the V, III-15, and I-10 protein segments.
Abstract: Fibronectin is an extracellular matrix glycoprotein encoded by a single gene. Alternative RNA splicing has been reported at three sites, ED (extra type III domain)-A, ED-B, and the variable or V region. Articular cartilage fibronectin monomers are rarely (ED-A)+, but approximately 25% are (ED-B)+. RNA gel electrophoresis and Northern blot analysis identified two (ED-B)+ and two (ED-B)- fibronectin transcripts in cartilage, each pair differing by approximately 750 bases. This difference results from a previously unreported RNA splicing pattern that eliminates not only the V region but also nucleotides encoding protein segments III-15 and I-10. This new splice variant, which we designate (V+C)-, represents the majority of fibronectin transcripts in equine, canine, and rabbit articular cartilage but is absent in the liver. Reverse transcriptase-polymerase chain reaction analyses of 11 additional equine tissues failed to detect the (V+C)- splice variant, except for very low levels in lymph node, bone, aorta, and skin. Furthermore, chondrocytes grown in monolayer culture maintain high levels of fibronectin expression but stop expressing (V+C)- transcripts over time. The tissue-specific expression pattern of this novel fibronectin isoform suggests that it may have an important function in the matrix organization of cartilage.
Publication Date: 1996-08-02 PubMed ID: 8702559DOI: 10.1074/jbc.271.31.18954Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates a specific variant of fibronectin mRNA in articular cartilage, which lacks certain protein sections. The study suggests that this variant, unique to cartilage and not seen in other tissues such as the liver, might play a crucial role in the cartilage’s matrix organization.
What Fibronectin Is
- Fibronectin is a glycoprotein found in the extracellular matrix.
- It’s encoded by a single gene, which can produce distinct RNA splice variants, which affect the function and structure of fibronectin proteins.
Alternative RNA Splicing Sites and Current Findings
- The identified splice sites of this gene are known as ED-A, ED-B, and the V region.
- However, in articular cartilage, the fibronectin molecules seldom include the ED-A splice, but about 25% include the ED-B splice.
- RNA gel electrophoresis and Northern blot analysis have identified two each of ED-B positive and ED-B negative fibronectin transcripts.
- These pairs differ by around 750 bases due to a distinctive RNA splicing pattern that removes not only the V region but also the regions encoding protein segments III-15 and I-10.
The New Fibronectin Splice Variant
- This previously unseen splice variant has been named (V+C)-.
- It makes up the majority of fibronectin transcripts in equine, canine, and rabbit articular cartilage but is missing in the liver.
- Additional analysis with eleven other equine tissues failed to detect the (V+C)- splice variant, except in very low levels found in the lymph node, bone, aorta, and skin.
- Chondrocytes, when grown in monolayer culture, continue to express high levels of fibronectin but stop expressing the (V+C)- transcripts over time.
Potential Function of the Novel Fibronectin Variant
- The tissue-specific nature of the (V+C)- splice variant implies that it could play an important part in cartilage’s matrix organization.
- This could be a valuable area for further research, as understanding the role of this variant may lead to better understanding and potential treatments for diseases affecting the cartilage such as osteoarthritis.
Cite This Article
APA
MacLeod JN, Burton-Wurster N, Gu DN, Lust G.
(1996).
Fibronectin mRNA splice variant in articular cartilage lacks bases encoding the V, III-15, and I-10 protein segments.
J Biol Chem, 271(31), 18954-18960.
https://doi.org/10.1074/jbc.271.31.18954 Publication
Researcher Affiliations
- Department of Physiology, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
MeSH Terms
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Base Sequence
- Cartilage, Articular / metabolism
- DNA Primers / genetics
- DNA, Complementary / genetics
- Dogs
- Fibronectins / chemistry
- Fibronectins / genetics
- Genetic Variation
- Horses
- In Vitro Techniques
- Molecular Sequence Data
- Molecular Structure
- Polymerase Chain Reaction
- RNA, Messenger / genetics
- RNA, Messenger / metabolism
- Rabbits
- Sequence Homology, Nucleic Acid
- Species Specificity
Citations
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