First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil.
Abstract: The present study focuses on Cryptosporidium infections of foals in Brazil. A total of 92 animals of different breeds from 11 farms in the vicinity of Araçatuba in the state of São Paulo, were examined. According to PCR targeting the 18S rRNA gene, Cryptosporidium sp. DNA was detected in 21.7% (20/92) of foals. Good quality 18S rRNA, actin, HSP70 and gp60 genes nPCR amplicons were obtained from five fecal samples. PCR amplification and sequencing of a fragment of the GP60 sporozoite surface glycoprotein gene revealed C. parvum genotypes IIaA18G3R1, IIaA15G2R1. Interestingly, we also detected in two foals a GP60 genotype related to the human parasite C. hominis.
Copyright © 2016 Elsevier B.V. All rights reserved.
Publication Date: 2016-12-02 PubMed ID: 28043388DOI: 10.1016/j.vetpar.2016.11.021Google Scholar: Lookup
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- Journal Article
Summary
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This research investigates Cryptosporidium infections in foals in Brazil, reporting the first instances of specific Cryptosporidium genotypes. Three different genotypes were identified: one related to the human parasite Cryptosporidium hominis, and two to Cryptosporidium parvum.
Research Context
- The study took place at 11 farms around Araçatuba in the state of São Paulo, Brazil.
- It examined 92 foals of various breeds to establish the extent of Cryptosporidium infections.
Methodology
- A PCR (Polymerase Chain Reaction) analysis was conducted, specifically targeting the 18S rRNA gene, to detect any presence of Cryptosporidium DNA
- From the 92 foals, 20 tested positive for Cryptosporidium, which suggests a relatively high infection rate of around 21.7%.
- A high-quality nPCR (nested PCR) amplification was achieved for the actin, HSP70, gp60, and further 18S rRNA genes from five of the Cryptosporidium-positive fecal samples.
Findings
- Upon sequencing a fragment of the GP60 gene—which codes for a protein on the surface of Cryptosporidium’s infectious stage called the sporozoite—the researchers discovered three genotypes:
- One was related to Cryptosporidium hominis, a type of Cryptosporidium more commonly found infecting humans.
- The other two genotypes identified were related to Cryptosporidium parvum, a species well-known for causing infection in a range of animals, including humans.
- This is the first time these specific Cryptosporidium genotypes have been identified in foals in Brazil, highlighting new insight into the epidemiology of Cryptosporidium infections.
Significance
- This detection of the Cryptosporidium hominis genotype in foals may suggest the potential for interspecies transmission, which could have important public health implications.
Cite This Article
APA
Inácio SV, Widmer G, de Brito RL, Zucatto AS, de Aquino MC, Oliveira BC, Nakamura AA, Neto LD, Carvalho JG, Gomes JF, Meireles MV, Bresciani KD.
(2016).
First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil.
Vet Parasitol, 233, 48-51.
https://doi.org/10.1016/j.vetpar.2016.11.021 Publication
Researcher Affiliations
- Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, São Paulo, Brazil. Electronic address: sandra_byol@yahoo.com.br.
- Department of Infectious Disease & Global Health, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA, USA. Electronic address: giovanni.widmer@tufts.edu.
- Universidade Estadual Paulista (UNESP), de Ciências Agrárias e Veterinárias, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Jaboticabal, São Paulo, Brazil. Electronic address: rolomonte@gmail.com.
- Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, São Paulo, Brazil. Electronic address: anaszucatto@hotmail.com.
- Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, São Paulo, Brazil. Electronic address: monallyaquino@hotmail.com.
- Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, São Paulo, Brazil. Electronic address: bruno.9988@hotmail.com.
- Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, São Paulo, Brazil. Electronic address: akiravt@gmail.com.
- Universidade Estadual Paulista (UNESP), de Ciências Agrárias e Veterinárias, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Jaboticabal, São Paulo, Brazil. Electronic address: luiz.silveira@gmail.com.
- Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, São Paulo, Brazil. Electronic address: jgbcarvalho@uol.com.br.
- Universidade Estadual de Campinas (Unicamp), Institutos de Biologia e Computação, Campinas, São Paulo, Brazil. Electronic address: jgomes@ic.unicamp.br.
- Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, São Paulo, Brazil. Electronic address: marcelo@fmva.unesp.br.
- Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, São Paulo, Brazil. Electronic address: bresciani@fmva.unesp.br.
MeSH Terms
- Animals
- Brazil
- Cryptosporidiosis / parasitology
- Cryptosporidium / genetics
- Cryptosporidium parvum / genetics
- DNA, Protozoan / genetics
- Genotype
- HSP70 Heat-Shock Proteins / genetics
- Horses
- RNA, Ribosomal, 18S / genetics
Citations
This article has been cited 12 times.- Li XM, Geng HL, Wei YJ, Yan WL, Liu J, Wei XY, Zhang M, Wang XY, Zhang XX, Liu G. Global prevalence and risk factors of Cryptosporidium infection in Equus: A systematic review and meta-analysis. Front Cell Infect Microbiol 2022;12:1072385.
- Menu E, Mosnier E, Cotrel A, Favennec L, Razakandrainibe R, Valot S, Blanchet D, Dalle F, Costa D, Gaillet M, Demar M, de Laval F. Cryptosporidiosis outbreak in Amazonia, French Guiana, 2018. PLoS Negl Trop Dis 2022 Jan;16(1):e0010068.
- Ryan U, Zahedi A, Feng Y, Xiao L. An Update on Zoonotic Cryptosporidium Species and Genotypes in Humans. Animals (Basel) 2021 Nov 19;11(11).
- Jang DH, Cho HC, Shin SU, Kim EM, Park YJ, Hwang S, Park J, Choi KS. Prevalence and distribution pattern of Cryptosporidium spp. among pre-weaned diarrheic calves in the Republic of Korea. PLoS One 2021;16(11):e0259824.
- Couso-Pérez S, de Limia FB, Ares-Mazás E, Gómez-Couso H. First report of zoonotic Cryptosporidium parvum GP60 subtypes IIaA15G2R1 and IIaA16G3R1 in wild ponies from the northern Iberian Peninsula. Parasitol Res 2020 Jan;119(1):249-254.
- Certad G, Follet J, Gantois N, Hammouma-Ghelboun O, Guyot K, Benamrouz-Vanneste S, Fréalle E, Seesao Y, Delaire B, Creusy C, Even G, Verrez-Bagnis V, Ryan U, Gay M, Aliouat-Denis C, Viscogliosi E. Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France. Front Microbiol 2019;10:1037.
- Razakandrainibe R, Diawara EHI, Costa D, Le Goff L, Lemeteil D, Ballet JJ, Gargala G, Favennec L. Common occurrence of Cryptosporidium hominis in asymptomatic and symptomatic calves in France. PLoS Negl Trop Dis 2018 Mar;12(3):e0006355.
- Feng Y, Xiao L. Molecular Epidemiology of Cryptosporidiosis in China. Front Microbiol 2017;8:1701.
- Deng L, Li W, Zhong Z, Gong C, Cao X, Song Y, Wang W, Huang X, Liu X, Hu Y, Fu H, He M, Wang Y, Zhang Y, Wu K, Peng G. Occurrence and Genetic Characteristics of Cryptosporidium hominis and Cryptosporidium andersoni in Horses from Southwestern China. J Eukaryot Microbiol 2017 Sep;64(5):716-720.
- Peng X, Wang X, Jian J, Zuo Q, Liu H, Wang Y, Su Y, Cao J, Jiang B, Shen Y. Investigation of Cryptosporidium spp. and Enterocytozoon bieneusi in free-ranged livestock on the southeastern Qinghai-Xizang Plateau, China. BMC Infect Dis 2025 Mar 13;25(1):356.
- Xu L, Fukuda Y, Murakoshi F, Alviola P, Masangkay J, Recuenco FC, Shehata A, Omatsu T, Bando H, Fujii H, Une Y, Kato K. Molecular characterization and zoonotic risk assessment of Cryptosporidium spp. in Philippine bats. Food Waterborne Parasitol 2025 Mar;38:e00249.
- Li P, Zhang B, Zhao Y, Chen R, Yu F, Qi M, Zhang Z. Molecular detection of Cryptosporidium in Alpine musk deer (Moschus chrysogaster) in Gansu Province, Northwest China. Parasitol Res 2024 Jun 3;123(6):231.
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