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First isolation of Actinobacillus genomospecies 2 in Japan.
Abstract: We describe here the first isolation of Actinobacillus genomospecies 2 in Japan. The isolate was found in a septicemic foal and characterized by phenotypic and genetic analyses, with the latter consisting of 16S rDNA nucleotide sequence analysis plus multilocus sequence analysis using three housekeeping genes, recN, rpoA and thdF, that have been proposed for use as a genomic tool in place of DNA-DNA hybridization.
Publication Date: 2015-12-12 PubMed ID: 26668165PubMed Central: PMC4873865DOI: 10.1292/jvms.15-0597Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
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Summary
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This research article reports on the first-ever isolation of Actinobacillus genomospecies 2 in Japan, found in a diseased young horse. It details how it was characterized, noting the use of genetic analyses including 16S rDNA nucleotide sequence analysis and multilocus sequence analysis with three specific housekeeping genes.
First Isolation of Actinobacillus Genomospecies 2
- The study revolves around the isolation of Actinobacillus genomospecies 2 in Japan, marking the first time this species has been identified in the country.
- The isolation of the bacteria was from a foal suffering from septicemia, a serious bloodstream infection that can lead to the failure of multiple body systems.
Characterization of the Isolate
- The isolated bacteria was characterized using both phenotypic and genetic analyses.
- Phenotypic analysis helps to understand the behavior and characteristics of an organism from an observational perspective, providing information about the microorganism’s visible traits such as structure, growth, and metabolism.
Genetic Analysis
- The genetic analysis consisted of multiple processes.
- The 16S rDNA nucleotide sequence analysis aids in identifying and comparing bacteria at the molecular level, offering insight into the genetic makeup of the organism.
- A multilocus sequence analysis was also carried out to provide a broad genetic overview of the organism. This analysis uses the nucleotide sequence data of several housekeeping genes, which are genes consistently expressed in most cells – in this case, three particular housekeeping genes were used, recN, rpoA, and thdF.
Use of Housekeeping Genes in Place of DNA-DNA Hybridization
- In addition to above-mentioned analysis methods, the study also proposes to use housekeeping genes as a genomic tool in place of DNA-DNA hybridization – a method traditionally used in defining bacterial species.
- This may help to refine and simplify the process of microbial species identification and description, which can be challenging given the complexity and variability of bacterial genetic structures.
Cite This Article
APA
Murakami M, Shimonishi Y, Hobo S, Niwa H, Ito H.
(2015).
First isolation of Actinobacillus genomospecies 2 in Japan.
J Vet Med Sci, 78(4), 701-703.
https://doi.org/10.1292/jvms.15-0597 Publication
Researcher Affiliations
- Kumamoto Central Livestock Hygiene Service Center, 1666 Jyonanmachi-Shizume, Minami-ku, Kumamoto 861-3205, Japan.
MeSH Terms
- Actinobacillus / classification
- Actinobacillus / isolation & purification
- Actinobacillus Infections / microbiology
- Actinobacillus Infections / veterinary
- Animals
- Animals, Newborn
- Bacteremia / microbiology
- Bacteremia / veterinary
- DNA, Bacterial
- Horse Diseases / microbiology
- Horses
- Japan
- Molecular Typing
- Phylogeny
- RNA, Ribosomal, 16S
References
This article includes 11 references
- Christensen H, Bisgaard M. Revised definition of Actinobacillus sensu stricto isolated from animals. A review with special emphasis on diagnosis.. Vet Microbiol 2004 Mar 26;99(1):13-30.
- Christensen H, Bisgaard M, Angen O, Olsen JE. Final classification of Bisgaard taxon 9 as Actinobacillus arthritidis sp. nov. and recognition of a novel genomospecies for equine strains of Actinobacillus lignieresii.. Int J Syst Evol Microbiol 2002 Jul;52(Pt 4):1239-46.
- Christensen H, Kuhnert P, Busse HJ, Frederiksen WC, Bisgaard M. Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae.. Int J Syst Evol Microbiol 2007 Jan;57(Pt 1):166-178.
- Dousse F, Thomann A, Brodard I, Korczak BM, Schlatter Y, Kuhnert P, Miserez R, Frey J. Routine phenotypic identification of bacterial species of the family Pasteurellaceae isolated from animals.. J Vet Diagn Invest 2008 Nov;20(6):716-24.
- Hiraishi A, Shin YK, Ueda Y, Sugiyama J. Automated sequencing of PCR-amplified 16S rDNA on ‘Hydrolink’ gels. J. Microbiol. Methods 19: 145–154.
- Kuhnert P, Korczak BM. Prediction of whole-genome DNA-DNA similarity, determination of G+C content and phylogenetic analysis within the family Pasteurellaceae by multilocus sequence analysis (MLSA).. Microbiology (Reading) 2006 Sep;152(Pt 9):2537-2548.
- Layman QD, Rezabek GB, Ramachandran A, Love BC, Confer AW. A retrospective study of equine actinobacillosis cases: 1999-2011.. J Vet Diagn Invest 2014 May;26(3):365-375.
- Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol. Today 33: 152–155.
- Stackebrandt E, Goebel BM. Taxonomic notes: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Evol. Microbiol. 44: 846–849.
- Stackebrandt E, Frederiksen W, Garrity GM, Grimont PAD, Kämpfer P, Maiden MCJ, Nesme X, Rosselló-Mora R, Swings J, Trüper HG, Vauterin L, Ward AC, Whitman WB. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology.. Int J Syst Evol Microbiol 2002 May;52(Pt 3):1043-1047.
- Tindall BJ, Rosselló-Móra R, Busse HJ, Ludwig W, Kämpfer P. Notes on the characterization of prokaryote strains for taxonomic purposes.. Int J Syst Evol Microbiol 2010 Jan;60(Pt 1):249-266.
Citations
This article has been cited 1 times.- Vereecke N, Vandekerckhove A, Theuns S, Haesebrouck F, Boyen F. Whole genome sequencing to study antimicrobial resistance and RTX virulence genes in equine Actinobacillus isolates. Vet Res 2023 Apr 5;54(1):33.
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