First isolation of Actinobacillus genomospecies 2 in Japan.
Abstract: We describe here the first isolation of Actinobacillus genomospecies 2 in Japan. The isolate was found in a septicemic foal and characterized by phenotypic and genetic analyses, with the latter consisting of 16S rDNA nucleotide sequence analysis plus multilocus sequence analysis using three housekeeping genes, recN, rpoA and thdF, that have been proposed for use as a genomic tool in place of DNA-DNA hybridization.
Publication Date: 2015-12-12 PubMed ID: 26668165PubMed Central: PMC4873865DOI: 10.1292/jvms.15-0597Google Scholar: Lookup
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Summary
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This research article reports on the first-ever isolation of Actinobacillus genomospecies 2 in Japan, found in a diseased young horse. It details how it was characterized, noting the use of genetic analyses including 16S rDNA nucleotide sequence analysis and multilocus sequence analysis with three specific housekeeping genes.
First Isolation of Actinobacillus Genomospecies 2
- The study revolves around the isolation of Actinobacillus genomospecies 2 in Japan, marking the first time this species has been identified in the country.
- The isolation of the bacteria was from a foal suffering from septicemia, a serious bloodstream infection that can lead to the failure of multiple body systems.
Characterization of the Isolate
- The isolated bacteria was characterized using both phenotypic and genetic analyses.
- Phenotypic analysis helps to understand the behavior and characteristics of an organism from an observational perspective, providing information about the microorganism’s visible traits such as structure, growth, and metabolism.
Genetic Analysis
- The genetic analysis consisted of multiple processes.
- The 16S rDNA nucleotide sequence analysis aids in identifying and comparing bacteria at the molecular level, offering insight into the genetic makeup of the organism.
- A multilocus sequence analysis was also carried out to provide a broad genetic overview of the organism. This analysis uses the nucleotide sequence data of several housekeeping genes, which are genes consistently expressed in most cells – in this case, three particular housekeeping genes were used, recN, rpoA, and thdF.
Use of Housekeeping Genes in Place of DNA-DNA Hybridization
- In addition to above-mentioned analysis methods, the study also proposes to use housekeeping genes as a genomic tool in place of DNA-DNA hybridization – a method traditionally used in defining bacterial species.
- This may help to refine and simplify the process of microbial species identification and description, which can be challenging given the complexity and variability of bacterial genetic structures.
Cite This Article
APA
Murakami M, Shimonishi Y, Hobo S, Niwa H, Ito H.
(2015).
First isolation of Actinobacillus genomospecies 2 in Japan.
J Vet Med Sci, 78(4), 701-703.
https://doi.org/10.1292/jvms.15-0597 Publication
Researcher Affiliations
- Kumamoto Central Livestock Hygiene Service Center, 1666 Jyonanmachi-Shizume, Minami-ku, Kumamoto 861-3205, Japan.
MeSH Terms
- Actinobacillus / classification
- Actinobacillus / isolation & purification
- Actinobacillus Infections / microbiology
- Actinobacillus Infections / veterinary
- Animals
- Animals, Newborn
- Bacteremia / microbiology
- Bacteremia / veterinary
- DNA, Bacterial
- Horse Diseases / microbiology
- Horses
- Japan
- Molecular Typing
- Phylogeny
- RNA, Ribosomal, 16S
References
This article includes 11 references
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Citations
This article has been cited 1 times.- Vereecke N, Vandekerckhove A, Theuns S, Haesebrouck F, Boyen F. Whole genome sequencing to study antimicrobial resistance and RTX virulence genes in equine Actinobacillus isolates. Vet Res 2023 Apr 5;54(1):33.
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