Flow cytometry analysis of CD11c-positive peripheral blood mononuclear cells in horses.
Abstract: Horses have played a prominent role in shaping our modern world, with important effects on health. Unfortunately, better characterization of the horse immune system is still needed. In this report, using flow cytometry techniques, four monoclonal antibodies against horse CD11c integrin were characterized and described for their ability to provide a positive recognition signal in peripheral blood mononuclear cells. Further immune cell phenotype experiments were performed using MHC-II, CD14, TLR4 and the specific anti-horse CD11c monoclonal antibody (1C4). With this staining panel, it was possible to detect a cell population defined by CD11c+MHC-II+TLR4+CD14low, which could be considered as putative dendritic cells. This manuscript shows that a new monoclonal antibody (1C4) can be used for the characterization of dendritic cells and their different lineages, opening the possibility of better understanding the mechanisms of immunity in horses.
Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
Publication Date: 2022-10-18 PubMed ID: 36327940DOI: 10.1016/j.vetimm.2022.110504Google Scholar: Lookup
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- Journal Article
Summary
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The study is about the use of a new monoclonal antibody to categorize a particular cell population in horses. The antibody can help in better understanding the horse immune system by recognizing and characterizing dendritic cells, a component of the immune system.
Research Goals and Methodology
- The primary aim of this research was to improve the understanding of the equine immune system by identifying and characterizing specific cells and proteins.
- To achieve this, four different kinds of monoclonal antibodies against a protein called CD11c were analyzed. This protein is found in specific immune cells of horses, and the antibodies are used to detect and interact with this protein.
- Flow cytometry, a technique used to measure physical and chemical characteristics of cells, was employed for this analysis.
Characterization of Monoclonal Antibodies
- The antibodies’ ability to identify CD11c in peripheral blood mononuclear cells, a type of immune cell found in blood, was examined.
- Certain antibodies, including one named 1C4, showed a positive recognition signal, which means they successfully bonded with the CD11c integrin in these blood cells.
Detection of Dendritic Cells
- Following the characterization of antibodies, the researchers conducted further tests to classify the immune cells. These cells were scanned for the presence of different markers, specifically MHC-II, CD14, TLR4, and the CD11c with the help of the newly identified anti-horse CD11c antibody (1C4).
- The cells that tested positive for the presence of CD11c, MHC-II, TLR4, and a low presence of CD14, were categorized as potential dendritic cells. Dendritic cells play a crucial role in the immune response by presenting antigens to T cells.
Implications of the Study
- The use of the newly characterized monoclonal antibody (1C4) for marking CD11c in horses allows a more precise detection and categorization of dendritic cells and their lineages.
- This tool opens opportunities for a deeper understanding of the immune systems in horses, and potentially the development of targeted treatments and vaccines against equine diseases.
Cite This Article
APA
Espinoza-Duarte MR, Ortega-Ochoa C, Baca-Ramirez A, Possani LD, Espino-Solis GP.
(2022).
Flow cytometry analysis of CD11c-positive peripheral blood mononuclear cells in horses.
Vet Immunol Immunopathol, 253, 110504.
https://doi.org/10.1016/j.vetimm.2022.110504 Publication
Researcher Affiliations
- Facultad de Medicina y Ciencias Biomédicas, Autonomous University of Chihuahua, Circuito Universitario s/n. Campus II, 31125 Chihuahua, Chih, Mexico; Laboratorio Nacional de Citometría de Flujo - Sede Chihuahua, Autonomous University of Chihuahua, Faculty of Medicine, Circuito Universitario s/n. Campus II, 31125 Chihuahua, Chih, Mexico.
- Facultad de Zootecnia y Ecologia, Periférico Fco. R. Almada km 1, Chihuahua 31453, Mexico.
- Facultad de Medicina y Ciencias Biomédicas, Autonomous University of Chihuahua, Circuito Universitario s/n. Campus II, 31125 Chihuahua, Chih, Mexico.
- Instituto de Biotecnología - UNAM, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, 62210 Cuernavaca, Morelos, Mexico.
- Facultad de Medicina y Ciencias Biomédicas, Autonomous University of Chihuahua, Circuito Universitario s/n. Campus II, 31125 Chihuahua, Chih, Mexico; Laboratorio Nacional de Citometría de Flujo - Sede Chihuahua, Autonomous University of Chihuahua, Faculty of Medicine, Circuito Universitario s/n. Campus II, 31125 Chihuahua, Chih, Mexico. Electronic address: gespinos@uach.mx.
MeSH Terms
- Animals
- Horses
- Flow Cytometry / veterinary
- Flow Cytometry / methods
- Leukocytes, Mononuclear
- Toll-Like Receptor 4
- CD11c Antigen
- Antibodies, Monoclonal
- Dendritic Cells
Citations
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