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The Journal of veterinary medical science2024; 86(8); 828-832; doi: 10.1292/jvms.23-0342

Genetic analysis based on next generation sequencing of Streptococcus equi subsp. equi isolated from horses imported into Japan.

Abstract: Strangles is a globally widespread, commonly diagnosed and important infectious disease of equids caused by Streptococcus equi subsp. equi. We performed whole genome sequencing of 19 S. equi isolates collected from imported horses at the Japanese border. Of these isolates, 15 isolates were obtained from clinical cases and 4 were from subclinical cases. The 19 isolates were grouped into 3 Bayesian analysis of population structure (BAPS) groups by the core genome single nucleotide polymorphism analysis corresponding to exporting country, SeM typing, or exporter of the horses. The 19 isolates possessed same pathogenic genes regardless of clinical status in imported horses and no antimicrobial resistance genes. The disease status of the horses may rather reflect the prior exposure of animals with sub-clinical infection to S. equi.
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Publication Date: 2024-06-18 PubMed ID: 38897953PubMed Central: PMC11300130DOI: 10.1292/jvms.23-0342Google Scholar: Lookup
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APA
Nakajima K, Kasuya K, Senba H, Tagami K, Kinoshita Y, Niwa H. (2024). Genetic analysis based on next generation sequencing of Streptococcus equi subsp. equi isolated from horses imported into Japan. J Vet Med Sci, 86(8), 828-832. https://doi.org/10.1292/jvms.23-0342

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 86
Issue: 8
Pages: 828-832

Researcher Affiliations

Nakajima, Kei
  • Moji Branch Shinmoji Quarantine Facility, Animal Quarantine Service, MAFF, Fukuoka, Japan.
Kasuya, Kazufumi
  • Microbiological Examination Division, Laboratory Department, Animal Quarantine Service, MAFF, Kanagawa, Japan.
Senba, Hironobu
  • Pathological and Physiochemical Examination Division, Laboratory Department, Animal Quarantine Service, MAFF, Kanagawa, Japan.
Tagami, Katsunori
  • Moji Branch Shinmoji Quarantine Facility, Animal Quarantine Service, MAFF, Fukuoka, Japan.
Kinoshita, Yuta
  • Microbiology Division, Equine Research Institute, Japan Racing Association, Tochigi, Japan.
Niwa, Hidekazu
  • Microbiology Division, Equine Research Institute, Japan Racing Association, Tochigi, Japan.

MeSH Terms

  • Horses
  • Animals
  • Horse Diseases / microbiology
  • Japan
  • Streptococcal Infections / veterinary
  • Streptococcal Infections / microbiology
  • High-Throughput Nucleotide Sequencing / veterinary
  • Polymorphism, Single Nucleotide
  • Whole Genome Sequencing
  • Streptococcus / genetics
  • Streptococcus / isolation & purification
  • Streptococcus equi / genetics
  • Streptococcus equi / isolation & purification
  • Genome, Bacterial

Conflict of Interest Statement

The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

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