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Home / Equine Research Bank / J Vet Med Sci / Genetic characteristics of feral Misaki horses based on poly...
The Journal of veterinary medical science2019; 81(5); 707-711; doi: 10.1292/jvms.18-0565

Genetic characteristics of feral Misaki horses based on polymorphisms of microsatellites and mitochondrial DNA.

Abstract: The Misaki horse is a Japanese native horse, known as the "feral horse of Cape Toi". In this study, we acquired the genetic information to establish their studbook, and analyzed their genetic characteristics for conservation. We genotyped 32 microsatellites and a mitochondrial D-loop region in 77 Misaki horses (80.2% of the population). The average number of alleles, observed heterozygosity, and expected heterozygosity were 3.4, 0.509, and 0.497, respectively. A neighbor-joining phylogenetic tree of individuals was constructed. Moreover, the results suggested that Misaki horses experienced a bottleneck, but it was neither severe nor recent. In addition, three mitochondrial haplotypes were confirmed. Consequently, we clarified the genetic background of Misaki horses that have been resident at Cape Toi for a long time.
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Publication Date: 2019-03-12 PubMed ID: 30867351PubMed Central: PMC6541847DOI: 10.1292/jvms.18-0565Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the genetic characteristics of Japanese native Misaki horses. DNA analysis is conducted with the goal of establishing their genetic diversity and history while also setting the groundwork for proper conservation efforts.

Research Objective

  • The study aimed to enhance the understanding of the genetic makeup of the Misaki horses using microsatellite and mitochondrial DNA polymorphisms.
  • Results from this research intended to guide future conservation efforts and breeding plans to preserve the genetic uniqueness of Misaki horses.

Methodology

  • The research involved 77 Misaki horses, equivalent to 80.2% of the living Misaki horse population.
  • The scientists used 32 microsatellites and a mitochondrial D-loop region for genotyping each horse.
  • The number of alleles, observed heterozygosity, and expected heterozygosity were calculated, with averages of 3.4, 0.509, and 0.497 respectively being obtained.
  • A neighbor-joining phylogenetic tree was constructed to map the genetic relationships among individuals.

Investigation Findings

  • The results suggested that the Misaki horses experienced a genetic bottleneck, a process that occurred when a significant percentage of their population was wiped out.
  • The bottleneck event, however, was neither severe nor recent, implying a sustained size of the Misaki horse population over more recent times.
  • Additionally, three different mitochondrial DNA haplotypes were confirmed in the horses, indicating the presence of different maternal lineages within the population.
  • The study thus elucidated on the genetic background of the Misaki horses that have been residing at Cape Toi for many generations.

Cite This Article

APA
Kobayashi I, Akita M, Takasu M, Tozaki T, Kakoi H, Nakamura K, Senju N, Matsuyama R, Horii Y. (2019). Genetic characteristics of feral Misaki horses based on polymorphisms of microsatellites and mitochondrial DNA. J Vet Med Sci, 81(5), 707-711. https://doi.org/10.1292/jvms.18-0565

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 81
Issue: 5
Pages: 707-711

Researcher Affiliations

Kobayashi, Ikuo
  • Sumiyoshi Livestock Science Station, Field Science Center, University of Miyazaki, 10100-1 Shimanouchi, Miyazaki 880-0121, Japan.
Akita, Masaru
  • Kushima City, 5550 Nishikata, Kushima, Miyazaki 888-8555, Japan.
Takasu, Masaki
  • Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
  • Education Center for Food Animal Health, Gifu University (GeFAH), 1-1 Yanagido, Gifu 501-1193, Japan.
Tozaki, Teruaki
  • Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
  • Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
Kakoi, Hironaga
  • Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
Nakamura, Kotono
  • Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Senju, Natsuko
  • Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Matsuyama, Ryota
  • Graduate School of Medicine, Hokkaido University, Kita 15 Jo Nishi 7 chome, Kita-ku, Sapporo, Hokkaido 060-8638, Japan.
Horii, Yoichiro
  • Department of Veterinary Medicine, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.

MeSH Terms

  • Animals
  • DNA, Mitochondrial / genetics
  • Female
  • Haplotypes
  • Horses / genetics
  • Japan
  • Male
  • Microsatellite Repeats / genetics
  • Pedigree
  • Polymorphism, Genetic

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Citations

This article has been cited 8 times.
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  2. Yoshihara T, Kikuchi M, Akiba Y, Horiguchi M, Takasu M, Tozaki T. Genetic diversity analysis and parentage verification of Taishu horses using 31 microsatellites. J Equine Sci 2022 Dec;33(4):63-69.
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  4. Nakamura M, Tozaki T, Kakoi H, Nakamura K, Rajabi-Toustani R, Ohba Y, Matsubara T, Takasu M. Decreased genetic diversity in Kiso horses revealed through annual microsatellite genotyping. J Vet Med Sci 2020 Apr 15;82(4):503-540.
    doi: 10.1292/jvms.19-0535pubmed: 32147602google scholar: lookup
  5. Hou L, Sulayman A, Zeng Y, Zhou L, Aimaier A, Kader A, Shi L. Analysis of Genetic Diversity and Race Genetic Structure of Major Horse Breeds in Xinjiang, China. Animals (Basel) 2025 Sep 14;15(18).
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