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Veterinary medicine international2010; 2010; 250978; doi: 10.4061/2010/250978

Histological and immunohistochemical evaluation of autologous cultured bone marrow mesenchymal stem cells and bone marrow mononucleated cells in collagenase-induced tendinitis of equine superficial digital flexor tendon.

Abstract: The aim of this study was to compare treatment with cultured bone marrow stromal cells (cBMSCs), bone marrow Mononucleated Cells (BMMNCs), and placebo to repair collagenase-induced tendinitis in horses. In six adult Standardbred horses, 4000 IU of collagenase were injected in the superficial digital flexor tendon (SDFT). Three weeks after collagenase treatment, an average of either 5.5 x 10(6) cBMSCs or 1.2 x 10(8) BMMNCs, fibrin glue, and saline solution was injected intralesionally in random order. In cBMSC- and BMMNCS-treated tendons, a high expression of cartilage oligomeric matrix protein (COMP) and type I collagen, but low levels of type III collagen were revealed by immunohistochemistry, with a normal longitudinally oriented fiber pattern. Placebo-treated tendons expressed very low quantities of COMP and type I collagen but large numbers of randomly oriented type III collagen fibers. Both cBMSC and BMMNCS grafts resulted in a qualitatively similar heling improvement of tendon extracellular matrix, in terms of the type I/III collagen ratio, fiber orientation, and COMP expression.
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Publication Date: 2010-03-22 PubMed ID: 20445779PubMed Central: PMC2859019DOI: 10.4061/2010/250978Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research conducted an experiment involving horses to evaluate the effectiveness of cultured bone marrow stromal cells (cBMSCs) and bone marrow Mononucleated Cells (BMMNCs) for treating tendinitis. The results showed that both approaches greatly improved the healing process and were superior compared to a placebo treatment.

Introduction and Methodology

  • The purpose of the research was to examine and contrast the effectiveness of two types of cell treatments, cultured bone marrow stromal cells (cBMSCs) and bone marrow Mononucleated Cells (BMMNCs), against a placebo in remedying equine tendinitis that was induced by collagenase.
  • In six adult Standardbred horses, the researchers started the process by inducing tendinitis in the superficial digital flexor tendon (SDFT) by injecting 4000 IU of collagenase.
  • Three weeks following the collagenase treatment, each horse received an intralesional injection which consisted of either 5.5 x 10(6) cBMSCs or 1.2 x 10(8) BMMNCs, fibrin glue, and saline solution, with the order being randomized for each horse.

Results

  • Immunohistochemical analysis was carried out on the treated tendons. Those which had been treated with cBMSCs and BMMNCs both showed good recovery markers: these were high levels of both cartilage oligomeric matrix protein (COMP) and type I collagen, which are associated with tissue healing.
  • The same tendons showed low amounts of type III collagen, which is a fibrillary collagen associated with scar tissue and is thus considered an undesirable component in healed tendons. The fiber pattern also returned to normal, in a longitudinally oriented manner.
  • On the other hand, the placebo-treated tendons did not demonstrate these promising results – they showed exceptionally low levels of COMP and type I collagen, but a significant presence of type III collagen fibers, which were also randomly oriented, indicating poor healing.

Conclusion

  • The study concluded that both cBMSC and BMMNCS were significantly better than the placebo treatment in healing the tendon extracellular matrix.
  • Both treatments provided a similar level of healing in terms of the type I/III collagen ratio, fiber orientation, and COMP expression.
  • This study illustrates the potential use of these cellular therapies in the treatment of tendinitis in horses, although it should be reiterated that this was a small sample size and more extensive research would need to be conducted.

Cite This Article

APA
Crovace A, Lacitignola L, Rossi G, Francioso E. (2010). Histological and immunohistochemical evaluation of autologous cultured bone marrow mesenchymal stem cells and bone marrow mononucleated cells in collagenase-induced tendinitis of equine superficial digital flexor tendon. Vet Med Int, 2010, 250978. https://doi.org/10.4061/2010/250978

Publication

Veterinary medicine international
ISSN: 2042-0048
NlmUniqueID: 101524203
Country: United States
Language: English
Volume: 2010
Pages: 250978
PII: 250978

Researcher Affiliations

Crovace, Antonio
  • Dipartimento dell'Emergenza e dei Trapianti di Organi (D.E.T.O.), Sezione di Chirurgia Veterinaria, Facoltà di Medicina Veterinaria, Università degli Studi di Bari, s.p. per Casamassima Km 3, Valenzano, 70010 Bari, Italy.
Lacitignola, Luca
    Rossi, Giacomo
      Francioso, Edda

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