Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: a randomized controlled trial.
- Journal Article
- Randomized Controlled Trial
- Research Support
- Non-U.S. Gov't
Summary
This research article provides an experimental study investigating the effectiveness and potential of using adipose-derived mesenchymal stem cells (adMSCs) and platelet concentrate (PC) in the treatment of tendonitis in equine subjects. The study concluded that this method is effective in preventing the progression of tendon injuries, reducing inflammation, and promoting better organization of collagen fibers.
Experimental Method and Procedure
The study involved eight mixed breed equines with artificially induced superficial digital flexor tendon lesions using a collagenase gel. After two weeks from the induction of the lesion, the researchers implemented the treatment – intralegional administration of adMSCs suspended in PC for the treated group and with phosphate buffered saline (PBS) for the control group. They conducted:
- Periodic ultrasound analyses every two weeks.
- A biopsy after 16 weeks for histopathological, immunohistochemical, and gene expression analyses which included type I collagen (COL1A1), type III collagen (COL3A1), tenascin-C (TNC), tenomodulin (TNMD), and scleraxis (SCX).
Major Findings
The results of both the treated and the control groups were compared and contrasted, showing significant differences. For the equines treated with adMSCs and PC:
- There was a notable decrease in the progression of the tendon lesion.
- Increased organization of collagen fibers was observed.
- Inflammation was significantly reduced.
- Ultrasound imaging demonstrated a lack of progression of the lesion area and its percentage.
- Power Doppler readings showed increased blood flow.
- No variation was found in the gene expression levels after the different treatments.
Conclusions
The study concluded that a combination of adMSCs and PC was successful in halting the progression of tendon injuries in equines, resulting in decreased inflammation and a better arrangement of collagen fibers. The implications of these results highlight the therapeutic potential of adMSCs and PC in treating equine tendonitis, although no significant changes were found in the gene expression levels of treated subjects. Overall, this experimental study contributes valuable insights and understanding towards the development of more effective treatments for equine tendon injuries.
Cite This Article
Publication
Researcher Affiliations
MeSH Terms
- Animals
- Blood Platelets / metabolism
- Cell- and Tissue-Based Therapy / methods
- Female
- Horse Diseases / pathology
- Horses
- Male
- Mesenchymal Stem Cells / metabolism
- Tendon Injuries / blood
- Tendon Injuries / therapy
References
- Kasashima Y, Takahashi T, Smith RK, Goodship AE, Kuwano A, Ueno T, Hirano S. Prevalence of superficial digital flexor tendonitis and suspensory desmitis in Japanese Thoroughbred flat racehorses in 1999.. Equine Vet J 2004 May;36(4):346-50.
- Avella CS, Ely ER, Verheyen KL, Price JS, Wood JL, Smith RK. Ultrasonographic assessment of the superficial digital flexor tendons of National Hunt racehorses in training over two racing seasons.. Equine Vet J 2009 May;41(5):449-54.
- Marfe G, Rotta G, De Martino L, Tafani M, Fiorito F, Di Stefano C, Polettini M, Ranalli M, Russo MA, Gambacurta A. A new clinical approach: use of blood-derived stem cells (BDSCs) for superficial digital flexor tendon injuries in horses.. Life Sci 2012 Jun 6;90(21-22):825-30.
- Nixon AJ, Dahlgren LA, Haupt JL, Yeager AE, Ward DL. Effect of adipose-derived nucleated cell fractions on tendon repair in horses with collagenase-induced tendinitis.. Am J Vet Res 2008 Jul;69(7):928-37.
- Carvalho A, Alves A, de Oliveira P, Alvarez L, Amorim R, Hussni C, Deffune E. Use of adipose tissue-derived mesenchymal stem cells for experimental tendinitis therapy in equines. J Equine Vet Sci. 2011;31:26u201334. doi: 10.1016/j.jevs.2010.11.014.
- Smith RK, Korda M, Blunn GW, Goodship AE. Isolation and implantation of autologous equine mesenchymal stem cells from bone marrow into the superficial digital flexor tendon as a potential novel treatment.. Equine Vet J 2003 Jan;35(1):99-102.
- Crovace A, Lacitignola L, De Siena R, Rossi G, Francioso E. Cell therapy for tendon repair in horses: an experimental study.. Vet Res Commun 2007 Aug;31 Suppl 1:281-3.
- Crovace A, Lacitignola L, Rossi G, Francioso E. Histological and immunohistochemical evaluation of autologous cultured bone marrow mesenchymal stem cells and bone marrow mononucleated cells in collagenase-induced tendinitis of equine superficial digital flexor tendon.. Vet Med Int 2010;2010:250978.
- Lacitignola L, Crovace A, Rossi G, Francioso E. Cell therapy for tendinitis, experimental and clinical report.. Vet Res Commun 2008 Sep;32 Suppl 1:S33-8.
- Pacini S, Spinabella S, Trombi L, Fazzi R, Galimberti S, Dini F, Carlucci F, Petrini M. Suspension of bone marrow-derived undifferentiated mesenchymal stromal cells for repair of superficial digital flexor tendon in race horses.. Tissue Eng 2007 Dec;13(12):2949-55.
- Del Bue M, Riccu00f2 S, Ramoni R, Conti V, Gnudi G, Grolli S. Equine adipose-tissue derived mesenchymal stem cells and platelet concentrates: their association in vitro and in vivo.. Vet Res Commun 2008 Sep;32 Suppl 1:S51-5.
- Bosch G, van Schie HT, de Groot MW, Cadby JA, van de Lest CH, Barneveld A, van Weeren PR. Effects of platelet-rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendons: A placebo-controlled experimental study.. J Orthop Res 2010 Feb;28(2):211-7.
- Schnabel LV, Lynch ME, van der Meulen MC, Yeager AE, Kornatowski MA, Nixon AJ. Mesenchymal stem cells and insulin-like growth factor-I gene-enhanced mesenchymal stem cells improve structural aspects of healing in equine flexor digitorum superficialis tendons.. J Orthop Res 2009 Oct;27(10):1392-8.
- Argu00fcelles D, Carmona JU, Climent F, Muu00f1oz E, Prades M. Autologous platelet concentrates as a treatment for musculoskeletal lesions in five horses.. Vet Rec 2008 Feb 16;162(7):208-11.
- Godwin EE, Young NJ, Dudhia J, Beamish IC, Smith RK. Implantation of bone marrow-derived mesenchymal stem cells demonstrates improved outcome in horses with overstrain injury of the superficial digital flexor tendon.. Equine Vet J 2012 Jan;44(1):25-32.
- Alves AL, de Mattos Carvalho A, Hussni CA. Mesenchymal stem cell therapy for equine tendinitis. Rec Pat Regen Med. 2013;3:103u2013110.
- Burk J, Ribitsch I, Gittel C, Juelke H, Kasper C, Staszyk C, Brehm W. Growth and differentiation characteristics of equine mesenchymal stromal cells derived from different sources.. Vet J 2013 Jan;195(1):98-106.
- Textor JA, Tablin F. Activation of equine platelet-rich plasma: comparison of methods and characterization of equine autologous thrombin.. Vet Surg 2012 Oct;41(7):784-94.
- Bosch G, Moleman M, Barneveld A, van Weeren PR, van Schie HT. The effect of platelet-rich plasma on the neovascularization of surgically created equine superficial digital flexor tendon lesions.. Scand J Med Sci Sports 2011 Aug;21(4):554-61.
- Filho Cerruti H, Kerkis I, Kerkis A, Tatsui NH, da Costa Neves A, Bueno DF, da Silva MC. Allogenous bone grafts improved by bone marrow stem cells and platelet growth factors: clinical case reports.. Artif Organs 2007 Apr;31(4):268-73.
- Pieri F, Lucarelli E, Corinaldesi G, Fini M, Aldini NN, Giardino R, Donati D, Marchetti C. Effect of mesenchymal stem cells and platelet-rich plasma on the healing of standardized bone defects in the alveolar ridge: a comparative histomorphometric study in minipigs.. J Oral Maxillofac Surg 2009 Feb;67(2):265-72.
- Yamada Y, Ueda M, Naiki T, Takahashi M, Hata K, Nagasaka T. Autogenous injectable bone for regeneration with mesenchymal stem cells and platelet-rich plasma: tissue-engineered bone regeneration.. Tissue Eng 2004 May-Jun;10(5-6):955-64.
- de Mattos Carvalho A, Alves AL, Golim MA, Moroz A, Hussni CA, de Oliveira PG, Deffune E. Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue.. Vet Immunol Immunopathol 2009 Dec 15;132(2-4):303-6.
- Watts AE, Nixon AJ, Yeager AE, Mohammed HO. A collagenase gel/physical defect model for controlled induction of superficial digital flexor tendonitis.. Equine Vet J 2012 Sep;44(5):576-86.
- Argu00fcelles D, Carmona JU, Pastor J, Iborra A, Viu00f1als L, Martu00ednez P, Bach E, Prades M. Evaluation of single and double centrifugation tube methods for concentrating equine platelets.. Res Vet Sci 2006 Oct;81(2):237-45.
- Watts AE, Yeager AE, Kopyov OV, Nixon AJ. Fetal derived embryonic-like stem cells improve healing in a large animal flexor tendonitis model.. Stem Cell Res Ther 2011 Jan 27;2(1):4.
- Risselada M, Kramer M, Saunders JH, Verleyen P, Van Bree H. Power Doppler assessment of the neovascularization during uncomplicated fracture healing of long bones in dogs and cats.. Vet Radiol Ultrasound 2006 May-Jun;47(3):301-6.
- Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.. Methods 2001 Dec;25(4):402-8.
- Flaminio MJ, Borges AS, Nydam DV, Horohov DW, Hecker R, Matychak MB. The effect of CpG-ODN on antigen presenting cells of the foal.. J Immune Based Ther Vaccines 2007 Jan 25;5:1.
- Schramme M, Hunter S, Campbell N, Blikslager A, Smith R. A surgical tendonitis model in horses: technique, clinical, ultrasonographic and histological characterisation.. Vet Comp Orthop Traumatol 2010;23(4):231-9.
- Lake SP, Ansorge HL, Soslowsky LJ. Animal models of tendinopathy.. Disabil Rehabil 2008;30(20-22):1530-41.
- Dirks RC, Warden SJ. Models for the study of tendinopathy.. J Musculoskelet Neuronal Interact 2011 Jun;11(2):141-9.
- Vidal MA, Kilroy GE, Lopez MJ, Johnson JR, Moore RM, Gimble JM. Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells.. Vet Surg 2007 Oct;36(7):613-22.
- Smith RK, Webbon PM. Harnessing the stem cell for the treatment of tendon injuries: heralding a new dawn?. Br J Sports Med 2005 Sep;39(9):582-4.
- Caniglia CJ, Schramme MC, Smith RK. The effect of intralesional injection of bone marrow derived mesenchymal stem cells and bone marrow supernatant on collagen fibril size in a surgical model of equine superficial digital flexor tendonitis.. Equine Vet J 2012 Sep;44(5):587-93.
- Boswell SG, Cole BJ, Sundman EA, Karas V, Fortier LA. Platelet-rich plasma: a milieu of bioactive factors.. Arthroscopy 2012 Mar;28(3):429-39.
- Barreira AP, Alves AL, Saito M, Amorim R, Kohayagawa A, Menarim B, Mota L. Autologous implant of bone marrow mononuclear cells as treatment of induced equine tendinitis. Intern J Appl Res Vet Med. 2008;6:46u201354.
- Bendinelli P, Matteucci E, Dogliotti G, Corsi MM, Banfi G, Maroni P, Desiderio MA. Molecular basis of anti-inflammatory action of platelet-rich plasma on human chondrocytes: mechanisms of NF-u03baB inhibition via HGF.. J Cell Physiol 2010 Nov;225(3):757-66.
- Puissant B, Barreau C, Bourin P, Clavel C, Corre J, Bousquet C, Taureau C, Cousin B, Abbal M, Laharrague P, Penicaud L, Casteilla L, Blancher A. Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells.. Br J Haematol 2005 Apr;129(1):118-29.
- Smith R. Tendon and Ligament Injury. Proceedings of the 54th Annual Convention of the American Association of Equine Practitioners 2008; San Diego, California. 2008. pp. 475u2013501.
- Salgado AJ, Reis RL, Sousa NJ, Gimble JM. Adipose tissue derived stem cells secretome: soluble factors and their roles in regenerative medicine.. Curr Stem Cell Res Ther 2010 Jun;5(2):103-10.
- Crop MJ, Baan CC, Korevaar SS, Ijzermans JN, Weimar W, Hoogduijn MJ. Human adipose tissue-derived mesenchymal stem cells induce explosive T-cell proliferation.. Stem Cells Dev 2010 Dec;19(12):1843-53.
- Nixon AJ, Watts AE, Schnabel LV. Cell- and gene-based approaches to tendon regeneration.. J Shoulder Elbow Surg 2012 Feb;21(2):278-94.
- Rosenberg JB, Greengard JS, Montgomery RR. Genetic induction of a releasable pool of factor VIII in human endothelial cells.. Arterioscler Thromb Vasc Biol 2000 Dec;20(12):2689-95.
- Maffulli N, Ewen SW, Waterston SW, Reaper J, Barrass V. Tenocytes from ruptured and tendinopathic achilles tendons produce greater quantities of type III collagen than tenocytes from normal achilles tendons. An in vitro model of human tendon healing.. Am J Sports Med 2000 Jul-Aug;28(4):499-505.
- Chiquet-Ehrismann R, Chiquet M. Tenascins: regulation and putative functions during pathological stress.. J Pathol 2003 Jul;200(4):488-99.
- Minamitani T, Ikuta T, Saito Y, Takebe G, Sato M, Sawa H, Nishimura T, Nakamura F, Takahashi K, Ariga H, Matsumoto K. Modulation of collagen fibrillogenesis by tenascin-X and type VI collagen.. Exp Cell Res 2004 Aug 1;298(1):305-15.
- Docheva D, Hunziker EB, Fu00e4ssler R, Brandau O. Tenomodulin is necessary for tenocyte proliferation and tendon maturation.. Mol Cell Biol 2005 Jan;25(2):699-705.
- Schweitzer R, Chyung JH, Murtaugh LC, Brent AE, Rosen V, Olson EN, Lassar A, Tabin CJ. Analysis of the tendon cell fate using Scleraxis, a specific marker for tendons and ligaments.. Development 2001 Oct;128(19):3855-66.
- Young M. Stem cell applications in tendon disorders: a clinical perspective.. Stem Cells Int 2012;2012:637836.
- Taylor SE, Vaughan-Thomas A, Clements DN, Pinchbeck G, Macrory LC, Smith RK, Clegg PD. Gene expression markers of tendon fibroblasts in normal and diseased tissue compared to monolayer and three dimensional culture systems.. BMC Musculoskelet Disord 2009 Feb 26;10:27.
- Patterson-Kane JC, Becker DL, Rich T. The pathogenesis of tendon microdamage in athletes: the horse as a natural model for basic cellular research.. J Comp Pathol 2012 Aug-Oct;147(2-3):227-47.
Citations
This article has been cited 39 times.- Li C, Wang J, Yang W, Yu K, Hong J, Ji X, Yao M, Li S, Lu J, Chen Y, Yan S, Wu H, Ma C, Yu X, Jiang G, Liu A. 3D-printed hydrogel particles containing PRP laden with TDSCs promote tendon repair in a rat model of tendinopathy.. J Nanobiotechnology 2023 Jun 3;21(1):177.
- Petrova V, Vachkova E. Outlook of Adipose-Derived Stem Cells: Challenges to Their Clinical Application in Horses.. Vet Sci 2023 May 12;10(5).
- Song K, Yang GM, Han J, Gil M, Dayem AA, Kim K, Lim KM, Kang GH, Kim S, Jang SB, Vellingiri B, Cho SG. Modulation of Osteogenic Differentiation of Adipose-Derived Stromal Cells by Co-Treatment with 3, 4'-Dihydroxyflavone, U0126, and N-Acetyl Cysteine.. Int J Stem Cells 2022 Aug 30;15(3):334-345.
- Uberti B, Plaza A, Henru00edquez C. Pre-conditioning Strategies for Mesenchymal Stromal/Stem Cells in Inflammatory Conditions of Livestock Species.. Front Vet Sci 2022;9:806069.
- Chun SW, Kim W, Lee SY, Lim CY, Kim K, Kim JG, Park CH, Hong SH, Yoo HJ, Chung SG. A randomized controlled trial of stem cell injection for tendon tear.. Sci Rep 2022 Jan 17;12(1):818.
- Mocchi M, Bari E, Dotti S, Villa R, Berni P, Conti V, Del Bue M, Squassino GP, Segale L, Ramoni R, Torre ML, Perteghella S, Grolli S. Canine Mesenchymal Cell Lyosecretome Production and Safety Evaluation after Allogenic Intraarticular Injection in Osteoarthritic Dogs.. Animals (Basel) 2021 Nov 15;11(11).
- de Souza JB, Rosa GDS, Rossi MC, Stievani FC, Pfeifer JPH, Krieck AMT, Bovolato ALC, Fonseca-Alves CE, Borru00e1s VA, Alves ALG. In Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Delivery.. Front Bioeng Biotechnol 2021;9:674581.
- Przu0105dka P, Buczak K, Frejlich E, Gu0105sior L, Suliga K, Kieu0142bowicz Z. The Role of Mesenchymal Stem Cells (MSCs) in Veterinary Medicine and Their Use in Musculoskeletal Disorders.. Biomolecules 2021 Aug 2;11(8).
- Harman RM, Marx C, Van de Walle GR. Translational Animal Models Provide Insight Into Mesenchymal Stromal Cell (MSC) Secretome Therapy.. Front Cell Dev Biol 2021;9:654885.
- Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases.. Animals (Basel) 2021 Jan 19;11(1).
- Bukowska J, Szu00f3stek-Mioduchowska AZ, Kopcewicz M, Walendzik K, Machciu0144ska S, Gawrou0144ska-Kozak B. Adipose-Derived Stromal/Stem Cells from Large Animal Models: from Basic to Applied Science.. Stem Cell Rev Rep 2021 Jun;17(3):719-738.
- Ribitsch I, Baptista PM, Lange-Consiglio A, Melotti L, Patruno M, Jenner F, Schnabl-Feichter E, Dutton LC, Connolly DJ, van Steenbeek FG, Dudhia J, Penning LC. Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do.. Front Bioeng Biotechnol 2020;8:972.
- Jankowski M, Dompe C, Sibiak R, Wu0105siatycz G, Mozdziak P, Jau015bkowski JM, Antosik P, Kempisty B, Dyszkiewicz-Konwiu0144ska M. In Vitro Cultures of Adipose-Derived Stem Cells: An Overview of Methods, Molecular Analyses, and Clinical Applications.. Cells 2020 Jul 27;9(8).
- Mocchi M, Dotti S, Bue MD, Villa R, Bari E, Perteghella S, Torre ML, Grolli S. Veterinary Regenerative Medicine for Musculoskeletal Disorders: Can Mesenchymal Stem/Stromal Cells and Their Secretome Be the New Frontier?. Cells 2020 Jun 11;9(6).
- Kim SE, Kim JG, Park K. Biomaterials for the Treatment of Tendon Injury.. Tissue Eng Regen Med 2019 Oct;16(5):467-477.
- Torres-Torrillas M, Rubio M, Damia E, Cuervo B, Del Romero A, Pelu00e1ez P, Chicharro D, Miguel L, Sopena JJ. Adipose-Derived Mesenchymal Stem Cells: A Promising Tool in the Treatment of Musculoskeletal Diseases.. Int J Mol Sci 2019 Jun 25;20(12).
- Shojaee A, Parham A. Strategies of tenogenic differentiation of equine stem cells for tendon repair: current status and challenges.. Stem Cell Res Ther 2019 Jun 18;10(1):181.
- Kornicka K, Geburek F, Ru00f6cken M, Marycz K. Stem Cells in Equine Veterinary Practice-Current Trends, Risks, and Perspectives.. J Clin Med 2019 May 14;8(5).
- Klymiuk MC, Balz N, Elashry MI, Heimann M, Wenisch S, Arnhold S. Exosomes isolation and identification from equine mesenchymal stem cells.. BMC Vet Res 2019 Jan 28;15(1):42.
- Abat F, Alfredson H, Cucchiarini M, Madry H, Marmotti A, Mouton C, Oliveira JM, Pereira H, Peretti GM, Spang C, Stephen J, van Bergen CJA, de Girolamo L. Current trends in tendinopathy: consensus of the ESSKA basic science committee. Part II: treatment options.. J Exp Orthop 2018 Sep 24;5(1):38.
- Kovac M, Litvin YA, Aliev RO, Zakirova EY, Rutland CS, Kiyasov AP, Rizvanov AA. Gene Therapy Using Plasmid DNA Encoding VEGF164 and FGF2 Genes: A Novel Treatment of Naturally Occurring Tendinitis and Desmitis in Horses.. Front Pharmacol 2018;9:978.
- Ahrberg AB, Horstmeier C, Berner D, Brehm W, Gittel C, Hillmann A, Josten C, Rossi G, Schubert S, Winter K, Burk J. Effects of mesenchymal stromal cells versus serum on tendon healing in a controlled experimental trial in an equine model.. BMC Musculoskelet Disord 2018 Jul 18;19(1):230.
- Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance.. Cell Transplant 2018 Jan;27(1):93-116.
- Beerts C, Suls M, Broeckx SY, Seys B, Vandenberghe A, Declercq J, Duchateau L, Vidal MA, Spaas JH. Tenogenically Induced Allogeneic Peripheral Blood Mesenchymal Stem Cells in Allogeneic Platelet-Rich Plasma: 2-Year Follow-up after Tendon or Ligament Treatment in Horses.. Front Vet Sci 2017;4:158.
- Watts AE, Millar NL, Platt J, Kitson SM, Akbar M, Rech R, Griffin J, Pool R, Hughes T, McInnes IB, Gilchrist DS. MicroRNA29a Treatment Improves Early Tendon Injury.. Mol Ther 2017 Oct 4;25(10):2415-2426.
- Geburek F, Roggel F, van Schie HTM, Beineke A, Estrada R, Weber K, Hellige M, Rohn K, Jagodzinski M, Welke B, Hurschler C, Conrad S, Skutella T, van de Lest C, van Weeren R, Stadler PM. Effect of single intralesional treatment of surgically induced equine superficial digital flexor tendon core lesions with adipose-derived mesenchymal stromal cells: a controlled experimental trial.. Stem Cell Res Ther 2017 Jun 5;8(1):129.
- Scharf A, Holmes SP, Thoresen M, Mumaw J, Stumpf A, Peroni J. MRI-Based Assessment of Intralesional Delivery of Bone Marrow-Derived Mesenchymal Stem Cells in a Model of Equine Tendonitis.. Stem Cells Int 2016;2016:8610964.
- Hofer HR, Tuan RS. Secreted trophic factors of mesenchymal stem cells support neurovascular and musculoskeletal therapies.. Stem Cell Res Ther 2016 Sep 9;7(1):131.
- Zhou Y, Wang JH. PRP Treatment Efficacy for Tendinopathy: A Review of Basic Science Studies.. Biomed Res Int 2016;2016:9103792.
- Markoski MM. Advances in the Use of Stem Cells in Veterinary Medicine: From Basic Research to Clinical Practice.. Scientifica (Cairo) 2016;2016:4516920.
- Mirza MH, Bommala P, Richbourg HA, Rademacher N, Kearney MT, Lopez MJ. Gait Changes Vary among Horses with Naturally Occurring Osteoarthritis Following Intra-articular Administration of Autologous Platelet-Rich Plasma.. Front Vet Sci 2016;3:29.
- Dex S, Lin D, Shukunami C, Docheva D. Tenogenic modulating insider factor: Systematic assessment on the functions of tenomodulin gene.. Gene 2016 Aug 1;587(1):1-17.
- Yu Y, Zhou Y, Cheng T, Lu X, Yu K, Zhou Y, Hong J, Chen Y. Hypoxia enhances tenocyte differentiation of adipose-derived mesenchymal stem cells by inducing hypoxia-inducible factor-1u03b1 in a co-culture system.. Cell Prolif 2016 Apr;49(2):173-84.
- Geburek F, Mundle K, Conrad S, Hellige M, Walliser U, van Schie HT, van Weeren R, Skutella T, Stadler PM. Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions--a pilot study.. Stem Cell Res Ther 2016 Feb 1;7:21.
- Balolong E, Lee S, Nemeno JG, Lee JI. Are They Really Stem Cells? Scrutinizing the Identity of Cells and the Quality of Reporting in the Use of Adipose Tissue-Derived Stem Cells.. Stem Cells Int 2016;2016:2302430.
- Gonzu00e1lez JC, Lu00f3pez C, u00c1lvarez ME, Pu00e9rez JE, Carmona JU. Autologous leukocyte-reduced platelet-rich plasma therapy for Achilles tendinopathy induced by collagenase in a rabbit model.. Sci Rep 2016 Jan 19;6:19623.
- Geburek F, Lietzau M, Beineke A, Rohn K, Stadler PM. Effect of a single injection of autologous conditioned serum (ACS) on tendon healing in equine naturally occurring tendinopathies.. Stem Cell Res Ther 2015 Jun 26;6(1):126.
- Arnhold S, Wenisch S. Adipose tissue derived mesenchymal stem cells for musculoskeletal repair in veterinary medicine.. Am J Stem Cells 2015;4(1):1-12.
- Maru0119dziak M, Marycz K, Lewandowski D, Siudziu0144ska A, u015amieszek A. Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)-a new approach in veterinary regenerative medicine.. In Vitro Cell Dev Biol Anim 2015 Mar;51(3):230-40.