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Home/Equine Research Bank/Vet Sci/Regenerative Medicine Applied to Musculoskeletal Diseases in...
Veterinary sciences2023; 10(12); 666; doi: 10.3390/vetsci10120666

Regenerative Medicine Applied to Musculoskeletal Diseases in Equines: A Systematic Review.

Abstract: Musculoskeletal injuries in horses have a great economic impact, predominantly affecting tendons, ligaments, and cartilage, which have limited natural regeneration. Cell therapy, which uses mesenchymal stem cells due to their tissue differentiation properties and anti-inflammatory and immunoregulatory effects, aims to restore damaged tissue. In this manuscript, we performed a systematic review using the Parsifal tool, searching the PubMed and Web of Science databases for articles on regenerative medicine for equine musculoskeletal injuries. Our review covers 17 experimental clinical studies categorized by the therapeutic approach used: platelet-rich plasma, conditioned autologous serum, mesenchymal stem cells, and secretome. These therapies reduce healing time, promote regeneration of fibrocartilaginous tissue, improve cellular organization, and improve joint functionality and sustainability. In conclusion, regenerative therapies using platelet-rich plasma, conditioned autologous serum, equine mesenchymal stem cells, and the emerging field of the secretome represent a promising and highly effective approach for the treatment of joint pathologies in horses, implying a valuable advance in equine healthcare.
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Publication Date: 2023-11-23 PubMed ID: 38133217PubMed Central: PMC10748126DOI: 10.3390/vetsci10120666Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers reviewed previous studies to understand the impact of regenerative therapies like platelet-rich plasma, conditioned autologous serum, and mesenchymal stem cells on musculoskeletal injuries in horses.

Objective of the Research

  • This research was conducted to understand the efficiency and affectivity of various regenerative therapies, particularly platelet-rich plasma, conditioned autologous serum, and mesenchymal stem cells for musculoskeletal injuries in horses. Musculoskeletal damages in horses are common and have a significant economic burden, given their limited natural regeneration capabilities. The researchers aimed to evaluate the possible advantages regenerative medicine could offer in managing such injuries.

Methodology

  • The researchers performed a systematic review of scientific literature available on PubMed and Web of Science databases. The review was conducted using Parsifal, a tool specifically designed for systematic literature reviews.
  • The article covers 17 different clinical studies, all focused on regenerative therapy treatments for musculoskeletal injuries in horses.

Findings from the Review

  • The review found that therapies such as platelet-rich plasma, conditioned autologous serum, mesenchymal stem cells, and the secretome were effective. They not only reduced the time taken for healing but also promoted regeneration of fibrocartilaginous tissue and improved the organization of the cells.
  • These therapies also significantly improved joint functionality and sustainability, enhancing the overall quality of life for the horses post-treatment.

Conclusions

  • In their conclusion, the researchers state that regenerative therapies hold a promising future in treating joint pathologies in horses. They imply a valuable advancement in equine healthcare.
  • Therapies using platelet-rich plasma, conditioned autologous serum, equine mesenchymal stem cells, and the emerging field of the secretome show high effectiveness in treating such injuries.

Cite This Article

APA
Pu00e9rez Fraile A, Gonzu00e1lez-Cubero E, Martu00ednez-Flu00f3rez S, Olivera ER, Villar-Suu00e1rez V. (2023). Regenerative Medicine Applied to Musculoskeletal Diseases in Equines: A Systematic Review. Vet Sci, 10(12), 666. https://doi.org/10.3390/vetsci10120666

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 10
Issue: 12
PII: 666

Researcher Affiliations

Pu00e9rez Fraile, Andrea
  • Department of Anatomy, Veterinary Faculty, Campus de Vegazana, Universidad de Leu00f3n, 24007 Leu00f3n, Spain.
Gonzu00e1lez-Cubero, Elsa
  • Department of Anatomy, Veterinary Faculty, Campus de Vegazana, Universidad de Leu00f3n, 24007 Leu00f3n, Spain.
Martu00ednez-Flu00f3rez, Susana
  • Department of Anatomy, Veterinary Faculty, Campus de Vegazana, Universidad de Leu00f3n, 24007 Leu00f3n, Spain.
  • Institute of Biomedicine (IBIOMED), Veterinary Faculty, Campus de Vegazana, Universidad de Leu00f3n, 24007 Leu00f3n, Spain.
Olivera, Elu00edas R
  • Department of Molecular Biology, Veterinary Faculty, Campus de Vegazana, Universidad de Leu00f3n, 24007 Leu00f3n, Spain.
Villar-Suu00e1rez, Vega
  • Department of Anatomy, Veterinary Faculty, Campus de Vegazana, Universidad de Leu00f3n, 24007 Leu00f3n, Spain.
  • Institute of Biomedicine (IBIOMED), Veterinary Faculty, Campus de Vegazana, Universidad de Leu00f3n, 24007 Leu00f3n, Spain.

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

References

This article includes 53 references
  1. van Weeren P.R., Back W. Musculoskeletal Disease in Aged Horses and Its Management. Vet. Clin. N. Am.-Equine Pract. 2016;32:229u2013247. doi: 10.1016/j.cveq.2016.04.003.
    doi: 10.1016/j.cveq.2016.04.003pubmed: 27449390google scholar: lookup
  2. Wayne Mcilwraith C., Fortier L.A., Frisbie D.D., Nixon A.J. Equine Models of Articular Cartilage Repair. Cartilage. 2011;2:317u2013326. doi: 10.1177/1947603511406531.
    doi: 10.1177/1947603511406531pmc: PMC4297134pubmed: 26069590google scholar: lookup
  3. Torricelli P., Fini M., Filardo G., Tschon M., Pischedda M., Pacorini A., Kon E., Giardino R. Regenerative Medicine for the Treatment of Musculoskeletal Overuse Injuries in Competition Horses. Int. Orthop. 2011;35:1569u20131576. doi: 10.1007/s00264-011-1237-3.
    doi: 10.1007/s00264-011-1237-3pmc: PMC3174295pubmed: 21394594google scholar: lookup
  4. Godwin E.E., Young N.J., Dudhia J., Beamish I.C., Smith R.K.W. Implantation of Bone Marrow-Derived Mesenchymal Stem Cells Demonstrates Improved Outcome in Horses with Overstrain Injury of the Superficial Digital Flexor Tendon. Equine Vet. J. 2012;44:25u201332. doi: 10.1111/j.2042-3306.2011.00363.x.
    doi: 10.1111/j.2042-3306.2011.00363.xpubmed: 21615465google scholar: lookup
  5. Frisbie D.D., Smith R.K.W. Clinical Update on the Use of Mesenchymal Stem Cells in Equine Orthopaedics. Equine Vet. J. 2010;42:86u201389. doi: 10.2746/042516409X477263.
    doi: 10.2746/042516409X477263pubmed: 20121921google scholar: lookup
  6. Bogers S.H. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know. Front. Vet. Sci. 2018;5:70. doi: 10.3389/fvets.2018.00070.
    doi: 10.3389/fvets.2018.00070pmc: PMC5911772pubmed: 29713634google scholar: lookup
  7. Wojdasiewicz P., Poniatowski u0141.A., Szukiewicz D. The Role of Inflammatory and Anti-Inflammatory Cytokines in the Pathogenesis of Osteoarthritis. Mediat. Inflamm. 2014;2014:561459. doi: 10.1155/2014/561459.
    doi: 10.1155/2014/561459pmc: PMC4021678pubmed: 24876674google scholar: lookup
  8. Goldring M.B., Otero M. Inflammation in Osteoarthritis. Curr. Opin. Rheumatol. 2011;23:471u2013478. doi: 10.1097/BOR.0b013e328349c2b1.
    doi: 10.1097/BOR.0b013e328349c2b1pmc: PMC3937875pubmed: 21788902google scholar: lookup
  9. Baccarin R.Y.A., Seidel S.R.T., Michelacci Y.M., Tokawa P.K.A., Oliveira T.M. Osteoarthritis: A Common Disease That Should Be Avoided in the Athletic Horseu2019s Life. Anim. Front. 2022;12:25u201336. doi: 10.1093/af/vfac026.
    doi: 10.1093/af/vfac026pmc: PMC9197312pubmed: 35711506google scholar: lookup
  10. Dakin S.G., Dudhia J., Smith R.K.W. Resolving an Inflammatory Concept: The Importance of Inflammation and Resolution in Tendinopathy. Vet. Immunol. Immunopathol. 2014;158:121u2013127. doi: 10.1016/j.vetimm.2014.01.007.
    doi: 10.1016/j.vetimm.2014.01.007pmc: PMC3991845pubmed: 24556326google scholar: lookup
  11. Hunter D.J., Bierma-Zeinstra S. Osteoarthritis. Lancet. 2019;393:1745u20131759. doi: 10.1016/S0140-6736(19)30417-9.
    doi: 10.1016/S0140-6736(19)30417-9pubmed: 31034380google scholar: lookup
  12. Brennan M.L., Arlt S.P., Belshaw Z., Buckley L., Corah L., Doit H., Fajt V.R., Grindlay D.J.C., Moberly H.K., Morrow L.D., et al. Critically Appraised Topics (CATs) in Veterinary Medicine: Applying Evidence in Clinical Practice. Front. Vet. Sci. 2020;7:314. doi: 10.3389/fvets.2020.00314.
    doi: 10.3389/fvets.2020.00314pmc: PMC7333781pubmed: 32695798google scholar: lookup
  13. Magni A., Agostoni P., Bonezzi C., Massazza G., Menu00e8 P., Savarino V., Fornasari D. Management of Osteoarthritis: Expert Opinion on NSAIDs. Pain Ther. 2021;10:783u2013808. doi: 10.1007/s40122-021-00260-1.
    doi: 10.1007/s40122-021-00260-1pmc: PMC8586433pubmed: 33876393google scholar: lookup
  14. Malone E.D. Managing Chronic Arthritis. Vet. Clin. N. Am.-Equine Pract. 2002;18:411u2013437. doi: 10.1016/S0749-0739(02)00024-X.
    doi: 10.1016/S0749-0739(02)00024-Xpubmed: 12516926google scholar: lookup
  15. Fendrick A.M., Greenberg B.P. A Review of the Benefits and Risks of Nonsteroidal Anti-Inflammatory Drugs in the Management of Mild-to-Moderate Osteoarthritis. Osteopath. Med. Prim. Care. 2009;3:1. doi: 10.1186/1750-4732-3-1.
    doi: 10.1186/1750-4732-3-1pmc: PMC2646740pubmed: 19126235google scholar: lookup
  16. Esteves C.L., Sheldrake T.A., Mesquita S.P., Pesu00e1ntez J.J., Menghini T., Dawson L., Pu00e9ault B., Donadeu F.X. Isolation and Characterization of Equine Native MSC Populations. Stem Cell Res. Ther. 2017;8:80. doi: 10.1186/s13287-017-0525-2.
    doi: 10.1186/s13287-017-0525-2pmc: PMC5395828pubmed: 28420427google scholar: lookup
  17. Mason C., Dunnill P. A Brief Definition of Regenerative Medicine. Regen. Med. 2008;3:1u20135. doi: 10.2217/17460751.3.1.1.
    doi: 10.2217/17460751.3.1.1pubmed: 18154457google scholar: lookup
  18. Ribitsch I., Oreff G.L., Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases. Animals. 2021;11:234. doi: 10.3390/ani11010234.
    doi: 10.3390/ani11010234pmc: PMC7832834pubmed: 33477808google scholar: lookup
  19. Pezzanite L.M., Chow L., Griffenhagen G.M., Bass L., Goodrich L.R., Impastato R., Dow S. Distinct Differences in Immunological Properties of Equine Orthobiologics Revealed by Functional and Transcriptomic Analysis Using an Activated Macrophage Readout System. Front. Vet. Sci. 2023;10:1109473. doi: 10.3389/fvets.2023.1109473.
    doi: 10.3389/fvets.2023.1109473pmc: PMC9978772pubmed: 36876001google scholar: lookup
  20. Choudhary R.K., Choudhary S. Stem Cells in Veterinary Science. Springer; Berlin/Heidelberg, Germany: 2022.
  21. Kawase T. Platelet-Rich Plasma and Its Derivatives as Promising Bioactive Materials for Regenerative Medicine: Basic Principles and Concepts Underlying Recent Advances. Odontology. 2015;103:126u2013135. doi: 10.1007/s10266-015-0209-2.
    doi: 10.1007/s10266-015-0209-2pubmed: 26040505google scholar: lookup
  22. Su00e1nchez M., Anitua E., Orive G., Mujika I., Andia I. Platelet-Rich Therapies in the Treatment of Orthopaedic Sport Injuries. Sports Med. 2009;39:345u2013354. doi: 10.2165/00007256-200939050-00002.
    doi: 10.2165/00007256-200939050-00002pubmed: 19402740google scholar: lookup
  23. Mishra A., Harmon K., Woodall J., Vieira A. Sports Medicine Applications of Platelet Rich Plasma. Curr. Pharm. Biotechnol. 2012;13:1185u20131195. doi: 10.2174/138920112800624283.
    doi: 10.2174/138920112800624283pubmed: 21740373google scholar: lookup
  24. Geburek F., Gaus M., van Schie H.T.M., Rohn K., Stadler P.M. Effect of Intralesional Platelet-Rich Plasma (PRP) Treatment on Clinical and Ultrasonographic Parameters in Equine Naturally Occurring Superficial Digital Flexor Tendinopathiesu2014A Randomized Prospective Controlled Clinical Trial. BMC Vet. Res. 2016;12:191. doi: 10.1186/s12917-016-0826-1.
    doi: 10.1186/s12917-016-0826-1pmc: PMC5015224pubmed: 27604193google scholar: lookup
  25. Wehling P., Moser C., Frisbied D., McIlwraith C.W., Kawcak C.E., Krauspe R., Reinecke J.A. Autologous Conditioned Serum in the Treatment of Orthopedic Diseases: The Orthokineu00ae Therapy. BioDrugs. 2007;21:323u2013332. doi: 10.2165/00063030-200721050-00004.
    doi: 10.2165/00063030-200721050-00004pubmed: 17896838google scholar: lookup
  26. Camargo Garbin L., Morris M.J. A Comparative Review of Autologous Conditioned Serum and Autologous Protein Solution for Treatment of Osteoarthritis in Horses. Front. Vet. Sci. 2021;8:602978. doi: 10.3389/fvets.2021.602978.
    doi: 10.3389/fvets.2021.602978pmc: PMC7933025pubmed: 33681323google scholar: lookup
  27. Linardi R.L., Dodson M.E., Moss K.L., King W.J., Ortved K.F. The Effect of Autologous Protein Solution on the Inflammatory Cascade in Stimulated Equine Chondrocytes. Front. Vet. Sci. 2019;6:64. doi: 10.3389/fvets.2019.00064.
    doi: 10.3389/fvets.2019.00064pmc: PMC6414419pubmed: 30895181google scholar: lookup
  28. Mahla R.S. Stem Cells Applications in Regenerative Medicine and Disease Therapeutics. Int. J. Cell Biol. 2016;2016:6940283. doi: 10.1155/2016/6940283.
    doi: 10.1155/2016/6940283pmc: PMC4969512pubmed: 27516776google scholar: lookup
  29. Barrachina L., Romero A., Zaragoza P., Rodellar C., Vu00e1zquez F.J. Practical Considerations for Clinical Use of Mesenchymal Stem Cells: From the Laboratory to the Horse. Vet. J. 2018;238:49u201357. doi: 10.1016/j.tvjl.2018.07.004.
    doi: 10.1016/j.tvjl.2018.07.004pubmed: 30103915google scholar: lookup
  30. Gittel C., Brehm W., Burk J., Juelke H., Staszyk C., Ribitsch I. Isolation of Equine Multipotent Mesenchymal Stromal Cells by Enzymatic Tissue Digestion or Explant Technique: Comparison of Cellular Properties. BMC Vet. Res. 2013;9:22. doi: 10.1186/1746-6148-9-221.
    doi: 10.1186/1746-6148-9-221pmc: PMC4228449pubmed: 24168625google scholar: lookup
  31. Smith R.K., Korda M., Blunn G.W., Goodship A.E. Isolation and Implantation of Autologous Equine Mesenchymal Stem Cells from Bone Marrow into the Superficial Digital Flexor Tendon as a Potential Novel Treatment. Equine Vet. J. 2003;35:99u2013102. doi: 10.2746/042516403775467388.
    doi: 10.2746/042516403775467388pubmed: 12553472google scholar: lookup
  32. Barker W.H.J., Smith M.R.W., Minshall G.J., Wright I.M. Soft tissue injuries of the tarsocrural joint: A retrospective analysis of 30 cases evaluated arthroscopically. Equine Vet. J. 2013;45:435u2013441. doi: 10.1111/j.2042-3306.2012.00685.x.
    doi: 10.1111/j.2042-3306.2012.00685.xpubmed: 23173847google scholar: lookup
  33. Nicpou0144 J., Marycz K., Grzesiak J. Therapeutic Effect of Adipose-Derived Mesenchymal Stem Cell Injection in Horses Suffering from Bone Spavin. Pol. J. Vet. Sci. 2013;16:753u2013754. doi: 10.2478/pjvs-2013-0107.
    doi: 10.2478/pjvs-2013-0107pubmed: 24597313google scholar: lookup
  34. Kearney C.M., Khatab S., van Buul G.M., Plomp S.G.M., Korthagen N.M., Labbertu00e9 M.C., Goodrich L.R., Kisiday J.D., Van Weeren P.R., van Osch G.J.V.M., et al. Treatment Effects of Intra-Articular Allogenic Mesenchymal Stem Cell Secretome in an Equine Model of Joint Inflammation. Front. Vet. Sci. 2022;9:907616. doi: 10.3389/fvets.2022.907616.
    doi: 10.3389/fvets.2022.907616pmc: PMC9257274pubmed: 35812845google scholar: lookup
  35. Lavoie J.R., Rosu-Myles M. Uncovering the Secretes of Mesenchymal Stem Cells. Biochimie. 2013;95:2212u20132221. doi: 10.1016/j.biochi.2013.06.017.
    doi: 10.1016/j.biochi.2013.06.017pubmed: 23810910google scholar: lookup
  36. Page M.J., McKenzie J.E., Bossuyt P.M., Boutron I., Hoffmann T.C., Mulrow C.D., Shamseer L., Tetzlaff J.M., Akl E.A., Brennan S.E., et al. The PRISMA 2020 Statement: An Updated Guideline for Reporting Systematic Reviews. BMJ. 2021;372:1u20139. doi: 10.1136/bmj.n71.
    doi: 10.1136/bmj.n71pmc: PMC8005924pubmed: 33782057google scholar: lookup
  37. Mayet A., Zablotski Y., Roth S.P., Brehm W., Troillet A. Systematic Review and Meta-Analysis of Positive Long-Term Effects after Intra-Articular Administration of Orthobiologic Therapeutics in Horses with Naturally Occurring Osteoarthritis. Front. Vet. Sci. 2023;10:1125695. doi: 10.3389/fvets.2023.1125695.
    doi: 10.3389/fvets.2023.1125695pmc: PMC9997849pubmed: 36908512google scholar: lookup
  38. Camargo Garbin L., Lopez C., Carmona J.U. A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade. Front. Vet. Sci. 2021;8:641818. doi: 10.3389/fvets.2021.641818.
    doi: 10.3389/fvets.2021.641818pmc: PMC8044532pubmed: 33869321google scholar: lookup
  39. Scala M., Lenarduzzi S., Spagnolo F., Trapasso M., Ottonello C., Muraglia A., Barla A., Strada P. Regenerative Medicine for the Treatment of Teno-Desmic Injuries of the Equine. A Series of 150 Horses Treated with Platelet-Derived Growth Factors. Vivo. 2014;28:1119u20131124.
    pubmed: 25398809
  40. Yamada A.L.M., Alvarenga M.L., Brandu00e3o J.S., Watanabe M.J., Rodrigues C.A., Hussni C.A., Alves A.L.G. Arcabouu00e7o de PRP-Gel Associado a Cu00e9lulas Tronco Mesenquimais: Uso Em Lesu00f5es Condrais Em Modelo Experimental Equino. Pesqui. Vet. Bras. 2016;36:461u2013467. doi: 10.1590/S0100-736X2016000600001.
    doi: 10.1590/S0100-736X2016000600001google scholar: lookup
  41. Beerts C., Suls M., Broeckx S.Y., Seys B., Vandenberghe A., Declercq J., Duchateau L., Vidal M.A., Spaas J.H. Tenogenically Induced Allogeneic Peripheral Blood Mesenchymal Stem Cells in Allogeneic Platelet-Rich Plasma: 2-Year Follow-up after Tendon or Ligament Treatment in Horses. Front. Vet. Sci. 2017;4:158. doi: 10.3389/fvets.2017.00158.
    doi: 10.3389/fvets.2017.00158pmc: PMC5622984pubmed: 29018808google scholar: lookup
  42. Georg R., Maria C., Gisela A., Bianca C. Autologous Conditioned Plasma as Therapy of Tendon and Ligament Lesions in Seven Horses. J. Vet. Sci. 2010;11:173u2013175. doi: 10.4142/jvs.2010.11.2.173.
    doi: 10.4142/jvs.2010.11.2.173pmc: PMC2873820pubmed: 20458161google scholar: lookup
  43. Geburek F., Lietzau M., Beineke A., Rohn K., Stadler P.M. Effect of a Single Injection of Autologous Conditioned Serum (ACS) on Tendon Healing in Equine Naturally Occurring Tendinopathies. Stem Cell Res. Ther. 2015;6:126. doi: 10.1186/s13287-015-0115-0.
    doi: 10.1186/s13287-015-0115-0pmc: PMC4513386pubmed: 26113022google scholar: lookup
  44. Marques-Smith P., Kallerud A.S., Johansen G.M., Boysen P., Jacobsen A.M., Reitan K.M., Henriksen M.M., Lu00f6fgren M., Fjordbakk C.T. Is Clinical Effect of Autologous Conditioned Serum in Spontaneously Occurring Equine Articular Lameness Related to ACS Cytokine Profile? BMC Vet. Res. 2020;16:181. doi: 10.1186/s12917-020-02391-7.
    doi: 10.1186/s12917-020-02391-7pmc: PMC7278142pubmed: 32513154google scholar: lookup
  45. Mokbel A.N., El Tookhy O.S., Shamaa A.A., Rashed L.A., Sabry D., El Sayed A.M. Homing and Reparative Effect of Intra-Articular Injection of Autologus Mesenchymal Stem Cells in Osteoarthritic Animal Model. BMC Musculoskelet. Disord. 2011;12:259. doi: 10.1186/1471-2474-12-259.
    doi: 10.1186/1471-2474-12-259pmc: PMC3232438pubmed: 22085445google scholar: lookup
  46. Carvalho A.D.M., Badial P.R., u00c1lvarez L.E.C., Yamada A.L.M., Borges A.S., Deffune E., Hussni C.A., Garcia Alves A.L. Equine Tendonitis Therapy Using Mesenchymal Stem Cells and Platelet Concentrates: A Randomized Controlled Trial. Stem Cell Res. Ther. 2013;4:85. doi: 10.1186/scrt236.
    doi: 10.1186/scrt236pmc: PMC3854756pubmed: 23876512google scholar: lookup
  47. Renzi S., Riccu00f2 S., Dotti S., Sesso L., Grolli S., Cornali M., Carlin S., Patruno M., Cinotti S., Ferrari M. Autologous bone marrow mesenchymal stromal cells for regeneration of injured equine ligaments and tendons: A clinical report. Res. Vet. Sci. 2013;95:272u2013277. doi: 10.1016/j.rvsc.2013.01.017.
    doi: 10.1016/j.rvsc.2013.01.017pubmed: 23419936google scholar: lookup
  48. Broeckx S., Zimmerman M., Crocetti S., Suls M., Mariu00ebn T., Ferguson S.J., Chiers K., Duchateau L., Franco-Obregu00f3n A., Wuertz K., et al. Regenerative Therapies for Equine Degenerative Joint Disease: A Preliminary Study. PLoS ONE. 2014;9:e85917. doi: 10.1371/journal.pone.0085917.
    doi: 10.1371/journal.pone.0085917pmc: PMC3896436pubmed: 24465787google scholar: lookup
  49. Gonzu00e1lez-Fernu00e1ndez M.L., Pu00e9rez-Castrillo S., Su00e1nchez-Lu00e1zaro J.A., Prieto-Fernu00e1ndez J.G., Lu00f3pez-Gonzu00e1lez M.E., Lobato-Pu00e9rez S., Colau00e7o B.J., Olivera E.R., Villar-Suu00e1rez V. Assessment of Regeneration in Meniscal Lesions by Use of Mesenchymal Stem Cells Derived from Equine Bone Marrow and Adipose Tissue. Am. J. Vet. Res. 2016;77:779u2013788. doi: 10.2460/ajvr.77.7.779.
    doi: 10.2460/ajvr.77.7.779pubmed: 27347833google scholar: lookup
  50. Villatoro A.J., Hermida-Prieto M., Fernu00e1ndez V., Fariu00f1as F., Alcoholado C., Isabel Rodru00edguez-Garcu00eda M., Mariu00f1as-Pardo L., Becerra J. Allogeneic Adipose-Derived Mesenchymal Stem Cells (Horse Allo 20) for the Treatment of Osteoarthritis-Associated Lameness in Horses: Characterization, Safety, and Efficacy of Intra-Articular Treatment. Stem Cells Dev. 2018;27:1147u20131160. doi: 10.1089/scd.2018.0074.
    doi: 10.1089/scd.2018.0074pubmed: 29978736google scholar: lookup
  51. Xing D., Kwong J., Yang Z., Hou Y., Zhang W., Ma B., Lin J. Intra-Articular Injection of Mesenchymal Stem Cells in Treating Knee Osteoarthritis: A Systematic Review of Animal Studies. Osteoarthr. Cartil. 2018;26:445u2013461. doi: 10.1016/j.joca.2018.01.010.
    doi: 10.1016/j.joca.2018.01.010pubmed: 29427723google scholar: lookup
  52. Makridakis M., Roubelakis M.G., Vlahou A. Stem Cells: Insights into the Secretome. Biochim. Biophys. Acta. 2013;1834:2380u20132384. doi: 10.1016/j.bbapap.2013.01.032.
    doi: 10.1016/j.bbapap.2013.01.032pubmed: 23376432google scholar: lookup
  53. Aru00e9valo-Turrubiarte M., Baratta M., Ponti G., Chiaradia E., Martignani E. Extracellular Vesicles from Equine Mesenchymal Stem Cells Decrease Inflammation Markers in Chondrocytes in Vitro. Equine Vet. J. 2022;54:1133u20131143. doi: 10.1111/evj.13537.
    doi: 10.1111/evj.13537pmc: PMC9787580pubmed: 34741769google scholar: lookup

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