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Effect of adipose-derived nucleated cell fractions on tendon repair in horses with collagenase-induced tendinitis.
Abstract: To assess the potential of adipose-derived nucleated cell (ADNC) fractions to improve tendon repair in horses with collagenase-induced tendinitis. Methods: 8 horses. Methods: Collagenase was used to induce tendinitis in the superficial digital flexor tendon of 1 forelimb in each horse. Four horses were treated by injection of autogenous ADNC fractions, and 4 control horses were injected with PBS solution. Healing was compared by weekly ultrasonographic evaluation. Horses were euthanatized at 6 weeks. Gross and histologic evaluation of tendon structure, fiber alignment, and collagen typing were used to define tendon architecture. Biochemical and molecular analyses of collagen, DNA, and proteoglycan and gene expression of collagen type I and type III, decorin, cartilage oligomeric matrix protein (COMP), and insulin-like growth factor-I were performed. Results: Ultrasonography revealed no difference in rate or quality of repair between groups. Histologic evaluation revealed a significant improvement in tendon fiber architecture; reductions in vascularity, inflammatory cell infiltrate, and collagen type III formation; and improvements in tendon fiber density and alignment in ADNC-treated tendons. Repair sites did not differ in DNA, proteoglycan, or total collagen content. Gene expression of collagen type I and type III in treated and control tendons were similar. Gene expression of COMP was significantly increased in ADNC-injected tendons. Conclusions: ADNC injection improved tendon organization in treated tendons. Although biochemical and molecular differences were less profound, tendons appeared architecturally improved after ADNC injection, which was corroborated by improved tendon COMP expression. Use of ADNC in horses with tendinitis appears warranted.
Publication Date: 2008-07-03 PubMed ID: 18593247DOI: 10.2460/ajvr.69.7.928Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study conducted research on the impact of using adipose-derived nucleated cell (ADNC) fractions on the healing process in horses with collagenase-induced tendinitis. It concludes that injecting ADNC can lead to improved tendon organization and increased expression of a key protein called COMP, which might make the use of ADNC a useful treatment for tendinitis in horses.
Research Background
- The study aimed to assess the effectiveness of using adipose-derived nucleated cell (ADNC) fractions in treating tendinitis in horses. Tendinitis was induced by injecting a collagenase solution into the superficial digital flexor tendon of a horse’s forelimb.
Methodology
- The research was conducted using 8 horses. Four had ADNC injected into their tendons, while the remaining four were injected with a PBS solution as a control.
- The study then observed healing progress in each group based on weekly ultrasonographical evaluations. After six weeks, the horses were euthanized for further examination.
- The tendon’s structure and fiber alignment were assessed using gross and histologic evaluations. Additional testing was done using molecular analyses and biochemical tests for collagen, DNA and proteoglycan content, along with the expression of various genes associated with tendinitis.
Results
- The study discovered that there were no differences identified through ultrasonography in the rate or quality of tendon repair between the groups.
- However, significantly improved tendon fiber architecture was observed in horses treated with the ADNC injections. This manifested as a reduction in vascularity, inflammatory cell infiltrate, and the formation of type III collagen. The tendon fiber’s density and alignment were also improved in the treated group.
- At a molecular level, the study found no significant difference between the treated and control groups in terms of DNA, proteoglycan or total collagen content. Similarly, the expressions of types I and III collagen were found to be almost identical in the two groups.
- However, the expression of COMP, a protein involved in tendon structure and health, were found to be significantly increased in the tendons treated with ADNC.
Conclusion
- Although there were no significant biochemical or molecular differences between the two groups, the study concludes on the basis of the observed architectural improvements in treated tendons and the increase in COMP expression that the treatment of tendinitis in horses with ADNC is warranted.
Cite This Article
APA
Nixon AJ, Dahlgren LA, Haupt JL, Yeager AE, Ward DL.
(2008).
Effect of adipose-derived nucleated cell fractions on tendon repair in horses with collagenase-induced tendinitis.
Am J Vet Res, 69(7), 928-937.
https://doi.org/10.2460/ajvr.69.7.928 Publication
Researcher Affiliations
- Comparative Orthopaedics Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853-6401, USA.
MeSH Terms
- Adipose Tissue / cytology
- Animals
- Cell Transplantation / methods
- Cell Transplantation / veterinary
- Collagen Type I / biosynthesis
- Collagen Type I / genetics
- Collagen Type I / immunology
- Collagen Type III / biosynthesis
- Collagen Type III / genetics
- Collagen Type III / immunology
- Decorin
- Extracellular Matrix Proteins / biosynthesis
- Extracellular Matrix Proteins / genetics
- Extracellular Matrix Proteins / immunology
- Glycoproteins / biosynthesis
- Glycoproteins / genetics
- Glycoproteins / immunology
- Horse Diseases / diagnostic imaging
- Horse Diseases / immunology
- Horse Diseases / therapy
- Horses
- Immunohistochemistry / veterinary
- Insulin-Like Growth Factor I / biosynthesis
- Insulin-Like Growth Factor I / genetics
- Insulin-Like Growth Factor I / immunology
- Matrilin Proteins
- Proteoglycans / biosynthesis
- Proteoglycans / genetics
- Proteoglycans / immunology
- RNA, Messenger / biosynthesis
- RNA, Messenger / genetics
- Random Allocation
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- Tendinopathy / diagnostic imaging
- Tendinopathy / immunology
- Tendinopathy / therapy
- Tendinopathy / veterinary
- Ultrasonography
Citations
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