Equine adipose-derived mesenchymal stem cells: phenotype and growth characteristics, gene expression profile and differentiation potentials.
Abstract: Because of the therapeutic application of stem cells (SCs), isolation and characterization of different types of SCs, especially mesenchymal stem cells (MSCs), have gained considerable attention in recent studies. Adipose tissue is an abundant and accessible source of MSCs which can be used for tissue engineering and in particular for treatment of musculoskeletal disorders. This study was aimed to isolate and culture equine adipose-derived MSCs (AT-MSCs) from little amounts of fat tissue samples and determine some of their biological characteristics. Methods: In this descriptive study, only 3-5 grams of fat tissue were collected from three crossbred mares. Immediately, cells were isolated by mechanical means and enzymatic digestion and were cultured in optimized conditions until passage 3 (P3). The cells at P3 were evaluated for proliferative capacities, expression of specific markers, and osteogenic, chondrogenic and adipogenic differentiation potentials. Results: Results showed that the isolated cells were plastic adherent with a fibroblast-like phenotype. AT-MSCs exhibited expression of mesenchymal cluster of differentiation (CD) markers (CD29, CD44 and CD90) and not major histocompatibility complex II (MHC-II) and CD34 (hematopoietic marker). Cellular differentiation assays demonstrated the chondrogenic, adipogenic and osteogenic potential of the isolated cells. Conclusions: Taken together, our findings reveal that equine MSCs can be obtained easily from little amounts of fat tissue which can be used in the future for regenerative purposes in veterinary medicine.
Publication Date: 2015-01-13 PubMed ID: 25685736PubMed Central: PMC4297484DOI: 10.22074/cellj.2015.491Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research article is about the isolation and characterization of mesenchymal stem cells (MSCs) from equine fat tissues and studying their growth behavior, gene expression, and differentiation potentials.
Isolation and Culture of Adipose-Derived MSCs
- In the study, adipose tissues weighing between 3-5 grams were obtained from three crossbred mares.
- Through mechanical procedures and enzymatic digestion, cells were isolated from these tissues.
- Then, these cells were cultured under optimal conditions until they reached the third passage (P3), a measure of how many times the cells have been subcultured or replicated.
Cell Evaluation and Characterization
- The isolated cells showed adherence to a plastic substrate and had a fibroblastic phenotype, a characteristic common to MSCs.
- The cells displayed the expression of certain cluster of differentiation (CD) markers—CD29, CD44, and CD90—which are distinctive indicators for MSCs.
- At the same time, these cells didn’t express the major histocompatibility complex II (MHC-II), and CD34, a marker for hematopoietic stem cells. This finding further confirms the cells as MSCs, not of hematopoietic origination.
Differentiation Potential of MSCs
- Cellular assays were conducted to evaluate the differentiation potentials of the isolated cells.
- The cells showed the capacity to differentiate into chondrogenic (cartilage-forming), adipogenic (fat-producing), and osteogenic (bone-forming) cell types.
Findings and Conclusions
- The research reveals that equine MSCs can be efficiently isolated from small amounts of adipose tissue.
- These adipose-derived MSCs displayed the appropriate characteristics of mesenchymal stem cells and showed potent ability to differentiate into a variety of cell types.
- The study concludes that this finding holds great future prospects for veterinary regenerative therapies.
Cite This Article
APA
Alipour F, Parham A, Kazemi Mehrjerdi H, Dehghani H.
(2015).
Equine adipose-derived mesenchymal stem cells: phenotype and growth characteristics, gene expression profile and differentiation potentials.
Cell J, 16(4), 456-465.
https://doi.org/10.22074/cellj.2015.491 Publication
Researcher Affiliations
- Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
- Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran ; Embryonic and Stem Cell Biology and Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
- Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
- Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran ; Embryonic and Stem Cell Biology and Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
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