FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Lipids1980; 15(9); 682-685; doi: 10.1007/BF02534019

Horse erythrocyte gangliosides: preparation of the major hematoside NeuNG1-Lac-Cer.

Abstract: A simple method for the isolation of hematoside NeuNG1-Lac-Cer from horse erythrocytes is described. An aliquot of the crude ganglioside fraction was labeled by tritiated sodium borohydride after mild periodate oxidation. The compounds obtained were used as radioactive tracers in column chromatography. Gangliosides were applied onto a silicic acid column and eluted stepwise by solvents of steadily increasing polarity. The major ganglioside, NeuNG1-Lac-Cer, was eluted in a high yield by the solvent mixture chloroform/methanol/water (60:35:8, v/v/v).
Get Full Text
Publication Date: 1980-09-01 PubMed ID: 7421423DOI: 10.1007/BF02534019Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study provides a straightforward method for isolating NeuNG1-Lac-Cer ganglioside from horse red blood cells.

Abstract Interpretation

In this research article abstract, the researchers describe a simple and effective method they developed to isolate a specific type of ganglioside, called NeuNG1-Lac-Cer, from erythrocytes (red blood cells) of a horse. They used a labeling and column chromatography process for the isolation. The solvent mixture chloroform/methanol/water provided a high yield of the major ganglioside.

Explanation of the Research Paper

Objective & Overview of the Method

The main aim of this research was to find a simple method to isolate NeuNG1-Lac-Cer ganglioside from horse red blood cells. The researchers designed a method that involved:

  • Labeling a crude ganglioside fraction
  • Applying labeled gangliosides to a silicic acid column
  • Eluting the gangliosides stepwise using solvents of increasing polarity

Procedure Details

  • An aliquot (portion) of the crude ganglioside fraction was first labeled by tritiated sodium borohydride following a mild periodate oxidation. This helps in tracing these molecules during the experiment.
  • These labeled gangliosides were then applied onto a chromatographic column filled with silicic acid, a widely used stationary phase in column chromatography.
  • The gangliosides were eluted step by step using solvents of increasing polarity, which helps separate components based on their retention times or the speed at which they pass through the column.

Outcome of the Procedure

  • The major ganglioside, NeuNG1-Lac-Cer, was successfully eluted by the solvent mixture of chloroform, methanol, and water (in a volume ratio of 60:35:8), suggesting this method was effective in isolating this specific ganglioside.
  • This method was noted for its effectiveness and simplicity which may make it a useful approach for research and applications where isolation of this specific ganglioside is required.

Cite This Article

APA
Maget-Dana R, Michalski JC. (1980). Horse erythrocyte gangliosides: preparation of the major hematoside NeuNG1-Lac-Cer. Lipids, 15(9), 682-685. https://doi.org/10.1007/BF02534019

Publication

Lipids
ISSN: 0024-4201
NlmUniqueID: 0060450
Country: United States
Language: English
Volume: 15
Issue: 9
Pages: 682-685

Researcher Affiliations

Maget-Dana, R
    Michalski, J C

      MeSH Terms

      • Animals
      • Chromatography, Thin Layer
      • Erythrocytes / analysis
      • G(M3) Ganglioside / blood
      • G(M3) Ganglioside / isolation & purification
      • Gangliosides / blood
      • Horses
      • Sialic Acids / analysis

      References

      This article includes 16 references
      1. Iwamori M, Nagai Y. A new chromatographic approach to the resolution of individual gangliosides. Ganglioside mapping.. Biochim Biophys Acta 1978 Feb 27;528(2):257-67.
        pubmed: 623779
      2. Puro K. Isolation of bovine kidney gangliosides.. Acta Chem Scand 1970;24(1):13-22.
        pubmed: 5463866doi: 10.3891/acta.chem.scand.24-0013google scholar: lookup
      3. Veh R, Corfield AP, Sander M, Schauer R. Neuraminic acid-specific modification and tritium labelling of gangliosides.. Biochim Biophys Acta 1976 Jan 18;486(1):145-60.
        pubmed: 188483doi: 10.1016/0005-2760(77)90079-0google scholar: lookup
      4. Tettamanti G, Bonali F, Marchesini S, Zambotti V. A new procedure for the extraction, purification and fractionation of brain gangliosides.. Biochim Biophys Acta 1973 Jan 19;296(1):160-70.
        pubmed: 4693502doi: 10.1016/0005-2760(73)90055-6google scholar: lookup
      5. Momoi T, Ando S, Magai Y. High resolution preparative column chromatographic system for gangliosides using DEAE-Sephadex and a new porus silica, Iatrobeads.. Biochim Biophys Acta 1976 Sep 27;441(3):488-97.
        pubmed: 184827
      6. Zanetta JP, Breckenridge WC, Vincendon G. Analysis of monosaccharides by gas-liquid chromatography of the O-methyl glycosides as trifluoroacetate derivatives. Application to glycoproteins and glycolipids.. J Chromatogr 1972 Jul 5;69(2):291-304.
        pubmed: 5039936doi: 10.1016/s0021-9673(00)92897-8google scholar: lookup
      7. Gahmberg CG, Hakomori SI. External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes.. J Biol Chem 1973 Jun 25;248(12):4311-7.
        pubmed: 4711609
      8. Siddiqui B, McCluer RH. Lipid components of sialosylgalactosylceramide of human brain.. J Lipid Res 1968 May;9(3):366-70.
        pubmed: 5646187
      9. Ledeen RW, Yu RK, Eng LF. Gangliosides of human myelin: sialosylgalactosylceramide (G7) as a major component.. J Neurochem 1973 Oct;21(4):829-39.
        pubmed: 4754859doi: 10.1111/j.1471-4159.1973.tb07527.xgoogle scholar: lookup
      10. Craven DA, Gehrke CW. Quantitative determination of N-acetylneuraminic acid by gas-liquid chromatography.. J Chromatogr 1968 Oct 22;37(3):414-21.
        pubmed: 5685232doi: 10.1016/s0021-9673(01)99137-xgoogle scholar: lookup
      11. HANDA S, YAMAKAWA T. CHEMISTRY OF LIPIDS OF POSTHEMOLYTIC RESIDUE OR STROMA OF ERYTHROCYTES. XII. CHEMICAL STRUCTURE AND CHROMATOGRAPHIC BEHAVIOUR OF HEMATOSIDES OBTAINED FROM EQUINE AND DOG ERYTHROCYTES.. Jpn J Exp Med 1964 Oct;34:293-304.
        pubmed: 14264338
      12. Hakomori S, Saito T. Isolation and characterization of a glycosphingolipid having a new sialic acid.. Biochemistry 1969 Dec;8(12):5082-8.
        pubmed: 5365794doi: 10.1021/bi00840a061google scholar: lookup
      13. Svennerholm L, Fredman P. A procedure for the quantitative isolation of brain gangliosides.. Biochim Biophys Acta 1980 Jan 18;617(1):97-109.
        pubmed: 7353026doi: 10.1016/0005-2760(80)90227-1google scholar: lookup
      14. Tanner MJ, Boxer DH. Separation and some properties of the major proteins of the human erythrocyte membrane.. Biochem J 1972 Sep;129(2):333-47.
        pubmed: 4643321doi: 10.1042/bj1290333google scholar: lookup
      15. FOLCH J, LEES M, SLOANE STANLEY GH. A simple method for the isolation and purification of total lipides from animal tissues.. J Biol Chem 1957 May;226(1):497-509.
        pubmed: 13428781
      16. Seyfried TN, Ando S, Yu RK. Isolation and characterization of human liver hematoside.. J Lipid Res 1978 Jul;19(5):538-43.
        pubmed: 670832

      Citations

      This article has been cited 0 times.
      Subscribe to our newsletter
      Join 100,127 horse owners like you who receive our email updates on horse health and nutrition.