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Home / Equine Research Bank / Lipids / Horse erythrocyte gangliosides: preparation of the major hem...
Lipids1980; 15(9); 682-685; doi: 10.1007/BF02534019

Horse erythrocyte gangliosides: preparation of the major hematoside NeuNG1-Lac-Cer.

Abstract: A simple method for the isolation of hematoside NeuNG1-Lac-Cer from horse erythrocytes is described. An aliquot of the crude ganglioside fraction was labeled by tritiated sodium borohydride after mild periodate oxidation. The compounds obtained were used as radioactive tracers in column chromatography. Gangliosides were applied onto a silicic acid column and eluted stepwise by solvents of steadily increasing polarity. The major ganglioside, NeuNG1-Lac-Cer, was eluted in a high yield by the solvent mixture chloroform/methanol/water (60:35:8, v/v/v).
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Publication Date: 1980-09-01 PubMed ID: 7421423DOI: 10.1007/BF02534019Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study provides a straightforward method for isolating NeuNG1-Lac-Cer ganglioside from horse red blood cells.

Abstract Interpretation

In this research article abstract, the researchers describe a simple and effective method they developed to isolate a specific type of ganglioside, called NeuNG1-Lac-Cer, from erythrocytes (red blood cells) of a horse. They used a labeling and column chromatography process for the isolation. The solvent mixture chloroform/methanol/water provided a high yield of the major ganglioside.

Explanation of the Research Paper

Objective & Overview of the Method

The main aim of this research was to find a simple method to isolate NeuNG1-Lac-Cer ganglioside from horse red blood cells. The researchers designed a method that involved:

  • Labeling a crude ganglioside fraction
  • Applying labeled gangliosides to a silicic acid column
  • Eluting the gangliosides stepwise using solvents of increasing polarity

Procedure Details

  • An aliquot (portion) of the crude ganglioside fraction was first labeled by tritiated sodium borohydride following a mild periodate oxidation. This helps in tracing these molecules during the experiment.
  • These labeled gangliosides were then applied onto a chromatographic column filled with silicic acid, a widely used stationary phase in column chromatography.
  • The gangliosides were eluted step by step using solvents of increasing polarity, which helps separate components based on their retention times or the speed at which they pass through the column.

Outcome of the Procedure

  • The major ganglioside, NeuNG1-Lac-Cer, was successfully eluted by the solvent mixture of chloroform, methanol, and water (in a volume ratio of 60:35:8), suggesting this method was effective in isolating this specific ganglioside.
  • This method was noted for its effectiveness and simplicity which may make it a useful approach for research and applications where isolation of this specific ganglioside is required.

Cite This Article

APA
Maget-Dana R, Michalski JC. (1980). Horse erythrocyte gangliosides: preparation of the major hematoside NeuNG1-Lac-Cer. Lipids, 15(9), 682-685. https://doi.org/10.1007/BF02534019

Publication

Lipids
ISSN: 0024-4201
NlmUniqueID: 0060450
Country: United States
Language: English
Volume: 15
Issue: 9
Pages: 682-685

Researcher Affiliations

Maget-Dana, R
    Michalski, J C

      MeSH Terms

      • Animals
      • Chromatography, Thin Layer
      • Erythrocytes / analysis
      • G(M3) Ganglioside / blood
      • G(M3) Ganglioside / isolation & purification
      • Gangliosides / blood
      • Horses
      • Sialic Acids / analysis

      References

      This article includes 16 references
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      Citations

      This article has been cited 0 times.
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