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Home / Equine Research Bank / Med Vet Entomol / Host-feeding preferences of Culex pipiens and its potential ...
Medical and veterinary entomology2025; doi: 10.1111/mve.12802

Host-feeding preferences of Culex pipiens and its potential significance for flavivirus transmission in the Camargue, France.

Abstract: The spread of the West Nile (WNV) and Usutu (USUV) flaviviruses in Europe in recent decades highlights the urgent need to understand the transmission networks of these pathogens as a basis for effective decision-making. These viruses are part of a complex disease cycle that involves birds as principal hosts and humans and horses as dead-end hosts. Our study aims to uncover the intricate relationships between the main mosquito vector of these viruses, Culex pipiens L. (Diptera: Culicidae) and its feeding preferences based on the forage ratio among several host species, primarily birds in a land-use gradient. We estimated the bird host potential to act as a host for flavivirus, the reservoir capacity index, based on forage ratios and potential host competence based on molecular prevalence. We sampled mosquitoes and, at the same time, conducted bird censuses in the Camargue region in southern France, where co-circulation of these viruses has been reported. Several localities were sampled along a land-use gradient in peri-urban, agricultural and natural areas from May to November 2021. We identified 55 vertebrate species in 110 engorged Cx. pipiens by PCR amplification and sequencing of mitochondrial 12S and 16S Ribosomal DNA genes. Culex pipiens feeds primarily on 51 bird species and secondarily on two mammals, one amphibian and one reptile. Based on forage ratios, we found a preference of Cx. pipiens in the Camargue for the order Passeriformes and, more specifically, for Columba livia domestica L. (Columbiformes: Columbidae) in agricultural areas, and for Passer domesticus/montanus L. (Passeriformes: Passeridae), in agricultural and peri-urban areas. The natural habitats had significantly higher forage ratio values than agricultural and peri-urban areas. We suggest that certain key species, such as Passer sp., Columba livia and Turdus sp., might be potentially considered locally relevant hosts for transmission in this area, as they are important for mosquito feeding and also potentially important hosts for flavivirus amplification. These data will be beneficial in understanding host-vector interactions and the relationships between bird communities, mosquito feeding preferences and emerging mosquito-borne diseases.
© 2025 The Author(s). Medical and Veterinary Entomology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.
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Publication Date: 2025-03-21 PubMed ID: 40118784DOI: 10.1111/mve.12802Google Scholar: Lookup
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APA
Rodríguez-Valencia V, Olive MM, Le Goff G, Faisse M, Bourel M, L'Ambert G, Vollot B, Tolsá-García MJ, Paupy C, Roiz D. (2025). Host-feeding preferences of Culex pipiens and its potential significance for flavivirus transmission in the Camargue, France. Med Vet Entomol. https://doi.org/10.1111/mve.12802

Publication

Medical and veterinary entomology
ISSN: 1365-2915
NlmUniqueID: 8708682
Country: England
Language: English

Researcher Affiliations

Rodríguez-Valencia, Víctor
  • MIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, France.
  • International Joint Laboratory ELDORADO, IRD/UNAM, Mexico City, Mexico.
Olive, Marie-Marie
  • MIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, France.
  • ASTRE, Univ. Montpellier, CIRAD, INRAE, Montpellier, France.
Le Goff, Gilbert
  • MIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, France.
Faisse, Marine
  • MIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, France.
Bourel, Marie
  • MIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, France.
L'Ambert, Grégory
  • Entente interdépartementale pour la démoustication du littoral méditerranéen (EID Méditerranée), Montpellier, France.
Vollot, Benjamin
  • MIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, France.
Tolsá-García, María José
  • MIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, France.
  • International Joint Laboratory ELDORADO, IRD/UNAM, Mexico City, Mexico.
Paupy, Christophe
  • MIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, France.
Roiz, David
  • MIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, France.
  • International Joint Laboratory ELDORADO, IRD/UNAM, Mexico City, Mexico.

Grant Funding

  • 2018-2019 Biodiversa joint call for research proposals under the Biodiversa 3ERA-net COFUND programme / Biodiversa
  • ANR-19-EB14-0001-04 / Agence Nationale de la Recherche
  • International doctoral scholarship / Conahcyt

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