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Scientific reports2024; 14(1); 27684; doi: 10.1038/s41598-024-75734-1

Identification of altered blood metabolic pathways in equines following ethyl pyruvate administration using non-targeted metabolomics.

Abstract: Ethyl pyruvate (EP) has emerged as a promising compound with potential therapeutic benefits attributed to its anti-inflammatory and antioxidant properties. This study aimed to understand the effects of EP on plasma metabolites and immune cells in horses, utilizing advanced liquid chromatography-mass spectrometry (LC-MS)-based metabolomics, quantitative polymerase chain reaction (qPCR), and blood chemistry analyses. Our comprehensive analysis detected 2,366 ions, and 126 metabolites were accurately identified. Remarkably, EP administration induced significant changes in 28 metabolites at 1 h and 11 metabolites at 8 h, highlighting its time-dependent impact on metabolic pathways such as phenylalanine and arginine biosynthesis. Moreover, EP significantly lowered the expression of inflammatory markers interleukin (IL)-6 and heme oxygenase (HO)-1, indicating its potential as an anti-inflammatory agent. Blood chemistry analysis revealed notable reductions in glucose and triglyceride levels. These findings demonstrate that EP is a substance with potential effects on pathways associated with inflammation, oxidative stress, and metabolic processes.
© 2024. The Author(s).
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Publication Date: 2024-11-12 PubMed ID: 39532936PubMed Central: PMC11557697DOI: 10.1038/s41598-024-75734-1Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article explores the effects of a potentially therapeutic compound, Ethyl Pyruvate (EP), on the blood metabolites and immune cells in horses. The study suggests that EP has time-dependent impacts on metabolic pathways and might exhibit anti-inflammatory properties.

Research Methods

  • This study used advanced liquid chromatography-mass spectrometry (LC-MS) based metabolomics, which is a technique used to identify and quantify cellular metabolites. This method helps in deconstructing the complex network of metabolic reactions and pathways.
  • Along with this, the researchers applied a quantitative polymerase chain reaction (qPCR), a method used for measuring the quantity of specific DNA sequences or genes in a sample.
  • Furthermore, a detailed blood chemistry analysis was performed to understand more granular impacts of EP on horses’ blood.

Key Findings

  • The researchers found a significant change in 28 metabolites at 1 hour and 11 metabolites at 8 hours after EP administration, highlighting that the impact of EP is time-dependent. This primarily affected metabolic pathways associated with the biosynthesis of phenylalanine and arginine, which are essential amino acids for mammals.
  • The study also revealed that EP significantly lowered the expression of inflammatory markers such as Interleukin 6 (IL-6) and Heme Oxygenase 1 (HO-1). These are mediating molecules in the body’s response to inflammation, suggesting that EP could act as an anti-inflammatory agent.
  • The blood chemistry analysis showed a noticeable reduction in glucose and triglyceride levels after the application of EP, implying potential effects on metabolic processes.

Implications of the Study

  • The findings provide new insights into the potential therapeutic properties of Ethyl Pyruvate (EP), particularly its anti-inflammatory and antioxidant effects.
  • It also stresses the impact of EP on specific metabolic pathways, which can be valuable knowledge for studying diseases linked with metabolic dysfunctions.
  • The successful application of integrated methods (LC-MS, qPCR, and blood chemistry analysis) used in this study also demonstrates their potential utility in future research into disease mechanisms and therapeutic agents.

Cite This Article

APA
Kwak YB, Seo SA, Kim M, Yoon J. (2024). Identification of altered blood metabolic pathways in equines following ethyl pyruvate administration using non-targeted metabolomics. Sci Rep, 14(1), 27684. https://doi.org/10.1038/s41598-024-75734-1

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 14
Issue: 1
Pages: 27684
PII: 27684

Researcher Affiliations

Kwak, Young Beom
  • Department of Pharmaceutical Engineering, Inje University, Gimhae, Republic of Korea.
Seo, Soo Ah
  • Department of Animal Science, College of Natural Resource & Life Science, Pusan National University, Busan, Republic of Korea.
  • Future Earth Research Institute, PNU JYS Science Academy, Pusan National University, Busan, 46241, Republic of Korea.
Kim, Myunghoo
  • Department of Animal Science, College of Natural Resource & Life Science, Pusan National University, Busan, Republic of Korea. kimmhmm3@gmail.com.
  • Future Earth Research Institute, PNU JYS Science Academy, Pusan National University, Busan, 46241, Republic of Korea. kimmhmm3@gmail.com.
Yoon, Jungho
  • Korea Racing Authority, Gwacheon, Republic of Korea. junghoy11@gmail.com.

MeSH Terms

  • Pyruvates / metabolism
  • Animals
  • Metabolomics / methods
  • Horses
  • Metabolic Networks and Pathways / drug effects
  • Anti-Inflammatory Agents / pharmacology
  • Chromatography, Liquid / methods
  • Oxidative Stress / drug effects
  • Metabolome / drug effects
  • Male
  • Interleukin-6 / blood
  • Interleukin-6 / metabolism

Conflict of Interest Statement

The authors declare no competing interests.

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