Identification of O-cetylated N-acylneuraminic acids by mass spectrometry.
Abstract: A number of O-acetylated N-acylneuraminic acids, isolated from submandibular glands of cow and horse and from horse erythrocytes, have been characterized by mass spectrometry. On the basis of the typical fragmentation patterns of the pertrimethylsilyl derivatives of the methyl esters of the compounds, they were identified as 4-O-acetyl-, 9-O-acetyl-, 4,9-di-O-acetyl-, and 7,9-di-O-acetyl N-acetylneuraminic acid, and 4-O-acetyl-and 9-O-acetyl-N-glycolylneuraminic acid.
Publication Date: 1975-05-01 PubMed ID: 1137847DOI: 10.1016/s0008-6215(00)87002-0Google Scholar: Lookup
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- Journal Article
Summary
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This research paper is about the identification of certain types of N-acylneuraminic acids, which were extracted from cow and horse glands and horse erythrocytes, using mass spectrometry. The analysis identified the compounds based on their specific fragmentation patterns.
Research Methodology and Experimentation
- The research was conducted with the focus on identifying O-acetylated N-acylneuraminic acids, a specific class of sialic acids. These compounds were isolated from the submandibular glands of cows and horses, as well as from horse erythrocytes.
- The technique used for characterizing these structures was mass spectrometry, a method commonly employed in the field of analytical chemistry for identifying the amount and type of chemicals present in a sample.
Findings and Identification
- The result of the mass spectrometry led to the discovery of a number of O-acetylated N-acylneuraminic acids. The compounds were differentiated based on their unique fragmentation patterns when treated with a pertrimethylsilyl derivative treatment.
- Specifically, they were identified as 4-O-acetyl-, 9-O-acetyl-, 4,9-di-O-acetyl-, and 7,9-di-O-acetyl N-acetylneuraminic acid, and 4-O-acetyl-and 9-O-acetyl-N-glycolylneuraminic acid. Each descriptor explicates the position of the O-acetyl groups on the N-acylneuraminic acid structure.
Significance of the Study
- The successful identification of these compounds contributes to our understanding of biochemical structures and functions. More specifically, it improves our knowledge of sialic acids, a class of sugars that are widely dispersed in animal tissues and play crucial roles in biological functions such as cell-to-cell interaction, host-pathogen interaction, and more.
- This study is significant as it demonstrates the effectiveness of mass spectrometry as an analytical method to elucidate the structure and function of biochemical substances. This research can serve as a basis for further studies involving sialic acids and related compounds.
Cite This Article
APA
Kamerling JP, Vliegenthart JF.
(1975).
Identification of O-cetylated N-acylneuraminic acids by mass spectrometry.
Carbohydr Res, 41, 7-17.
https://doi.org/10.1016/s0008-6215(00)87002-0 Publication
Researcher Affiliations
MeSH Terms
- Acetates
- Animals
- Cattle
- Chromatography, Gas
- Erythrocytes / analysis
- Horses
- Mass Spectrometry
- Organ Specificity
- Sialic Acids / analysis
- Sialic Acids / blood
- Species Specificity
- Structure-Activity Relationship
- Submandibular Gland / analysis
Citations
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