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Home / Equine Research Bank / Clin Vaccine Immunol / Identification of Western equine encephalitis virus structur...
Clinical and vaccine immunology : CVI2009; 17(1); 176-179; doi: 10.1128/CVI.00377-09

Identification of Western equine encephalitis virus structural proteins that confer protection after DNA vaccination.

Abstract: DNA vaccines encoding different portions of the structural proteins of western equine encephalitis virus were tested for the efficacy of their protection in a 100% lethal mouse model of the virus. The 6K-E1 structural protein encoded by the DNA vaccine conferred complete protection against challenge with the homologous strain and limited protection against challenge with a heterologous strain.
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Publication Date: 2009-11-18 PubMed ID: 19923571PubMed Central: PMC2812089DOI: 10.1128/CVI.00377-09Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about the evaluation of DNA vaccines encoding different parts of structural proteins of the Western equine encephalitis virus for their protective efficacy using a deadly mouse model of the virus.

Introduction

  • The study is focused on the Western equine encephalitis virus (WEEV), a mosquito-transmitted virus that can cause severe disease in humans and equines.
  • The structural proteins of WEEV, including the 6K-E1 protein, play crucial roles in the viral life cycle, and are believed to be potential targets for a vaccine.

Methods

  • The researchers used DNA vaccines encoding various components of WEEV’s structural proteins.
  • They tested the vaccines’ protective capacity using a 100% lethal mouse model of the virus. This means all mice in this model are expected to die from the infection, providing a rigorous testing ground for potential vaccines.

Findings

  • The DNA vaccine encoding the 6K-E1 structural protein of WEEV produced the most promising results.
  • This vaccine conferred complete protection against the identical (‘homologous’) strain of WEEV that the vaccine was designed to combat.
  • Moreover, the vaccine provided limited protection against a different (‘heterologous’) strain of WEEV, indicating some degree of cross-protection.

Conclusion

  • The findings suggest that the 6K-E1 structural protein of WEEV is a potential target for a vaccine against the virus.
  • Furthermore, the capacity of the vaccine to provide some degree of cross-protection could be an important attribute considering the genetic diversity of WEEV and related viruses.

Future Directions

  • The research could pave the way for further investigation into the potential of DNA vaccines in protecting against WEEV.
  • Other proteins encoded by WEEV may likewise be investigated as potential targets for vaccine development.

Cite This Article

APA
Gauci PJ, Wu JQ, Rayner GA, Barabé ND, Nagata LP, Proll DF. (2009). Identification of Western equine encephalitis virus structural proteins that confer protection after DNA vaccination. Clin Vaccine Immunol, 17(1), 176-179. https://doi.org/10.1128/CVI.00377-09

Publication

Clinical and vaccine immunology : CVI
ISSN: 1556-679X
NlmUniqueID: 101252125
Country: United States
Language: English
Volume: 17
Issue: 1
Pages: 176-179

Researcher Affiliations

Gauci, Penelope J
  • Biological Countermeasures, Human Protection and Performance Division, Defence Science & Technology Organisation, 506 Lorimer St., Fishermans Bend, VIC 3207, Australia. Penny.Gauci@dsto.defence.gov.au
Wu, Josh Q H
    Rayner, George A
      Barabé, Nicole D
        Nagata, Leslie P
          Proll, David F

            MeSH Terms

            • Animals
            • Antigens, Viral / genetics
            • Antigens, Viral / immunology
            • Disease Models, Animal
            • Encephalitis Virus, Western Equine / genetics
            • Encephalitis Virus, Western Equine / immunology
            • Encephalomyelitis, Equine / prevention & control
            • Female
            • Mice
            • Mice, Inbred BALB C
            • Survival Analysis
            • Vaccines, DNA / immunology
            • Viral Structural Proteins / genetics
            • Viral Structural Proteins / immunology
            • Viral Vaccines / immunology

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            Citations

            This article has been cited 5 times.
            1. Torres-Ruesta A, Chee RS, Ng LFP. Insights into Antibody-Mediated Alphavirus Immunity and Vaccine Development Landscape.. Microorganisms 2021 Apr 22;9(5).
              doi: 10.3390/microorganisms9050899pubmed: 33922370google scholar: lookup
            2. Stromberg ZR, Fischer W, Bradfute SB, Kubicek-Sutherland JZ, Hraber P. Vaccine Advances against Venezuelan, Eastern, and Western Equine Encephalitis Viruses.. Vaccines (Basel) 2020 Jun 3;8(2).
              doi: 10.3390/vaccines8020273pubmed: 32503232google scholar: lookup
            3. Dupuy LC, Richards MJ, Livingston BD, Hannaman D, Schmaljohn CS. A Multiagent Alphavirus DNA Vaccine Delivered by Intramuscular Electroporation Elicits Robust and Durable Virus-Specific Immune Responses in Mice and Rabbits and Completely Protects Mice against Lethal Venezuelan, Western, and Eastern Equine Encephalitis Virus Aerosol Challenges.. J Immunol Res 2018;2018:8521060.
              doi: 10.1155/2018/8521060pubmed: 29967804google scholar: lookup
            4. Phillips AT, Schountz T, Toth AM, Rico AB, Jarvis DL, Powers AM, Olson KE. Liposome-antigen-nucleic acid complexes protect mice from lethal challenge with western and eastern equine encephalitis viruses.. J Virol 2014 Feb;88(3):1771-80.
              doi: 10.1128/JVI.02297-13pubmed: 24257615google scholar: lookup
            5. Toth AM, Geisler C, Aumiller JJ, Jarvis DL. Factors affecting recombinant Western equine encephalitis virus glycoprotein production in the baculovirus system.. Protein Expr Purif 2011 Dec;80(2):274-82.
              doi: 10.1016/j.pep.2011.08.002pubmed: 21864686google scholar: lookup
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