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Home / Equine Research Bank / Vet Res / IgE and IgG antibodies in skin allergy of the horse.
Veterinary research2006; 37(6); 813-825; doi: 10.1051/vetres:2006039

IgE and IgG antibodies in skin allergy of the horse.

Abstract: In horses, allergies have been characterized by clinical signs and/or intradermal (i.d.) allergen testing. Our aim was to find the first direct evidence that immunoglobulin E (IgE) mediates equine allergy. In addition, we tested the hypothesis that immediate skin reactions in horses can also be mediated by IgG. Anti-IgE affinity columns were used to purify IgE from serum of one healthy horse and three horses affected with summer eczema, an allergic dermatitis which is believed to be induced by Culicoides midges. A modified Prausnitz-Küstner experiment was performed in four clinical healthy horses by i.d. injection of the purified serum IgE antibodies. The following day, Culicoides allergen was injected at the same sites. Skin reactions were not observed in response to allergen alone, and in two horses after stimulation at any previous IgE injection site. However, the other two horses showed an immediate skin reaction at the previous injection sites of IgE obtained from allergic horses. In addition, purified monoclonal antibodies to various equine immunoglobulin isotypes were injected i.d. into six healthy horses. Immediate skin reactions were observed in response to anti-IgE (6/6 horses) and anti-IgG(T) injections (5/6 horses). The specificities of both antibodies for IgE and IgG(T), respectively, were confirmed by enzyme linked immunosorbent assays. The results provide the first direct evidence that IgE mediates classical Type-I allergy in horses and plays a major role in the pathogenesis of summer eczema. The data also suggest that IgG(T) can bind to skin mast cells and might contribute to clinical allergy.
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Publication Date: 2006-09-15 PubMed ID: 16973120DOI: 10.1051/vetres:2006039Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studies the role of immunoglobulin E (IgE) and immunoglobulin G (IgG) antibodies in skin allergies in horses, particularly summer eczema, and provides evidence that IgE mediates the allergy and that IgG potentially contributes to it.

Objectives of the Research

  • The research aimed to find direct evidence that IgE mediates equine allergy.
  • The study also tested the hypothesis that immediate skin reactions in horses could be mediated by IgG.

Methodology

  • Anti-IgE affinity columns were used to purify IgE from the serum of one healthy horse and three horses afflicted with summer eczema, an allergic dermatitis believed to be induced by Culicoides midges.
  • A modified Prausnitz-Küstner experiment, which traditionally tests the transferability of immediate allergies, was conducted on four clinically healthy horses by intradermal injection of the purified serum IgE antibodies.
  • The following day, Culicoides allergen was injected at the same sites. Observations were made to examine if any skin reactions occurred.
  • Purified monoclonal antibodies to various equine immunoglobulin isotypes were also injected intradermally into six healthy horses, and any immediate skin reactions were observed.
  • The enzyme-linked immunosorbent assays were used to confirm the specificities of both antibodies for IgE and IgG.

Results of the Research

  • Immediate skin reactions were observed in two horses at the previous injection sites of IgE obtained from allergic horses, providing the first direct evidence that IgE mediates classical Type-I allergy in horses and significantly contributes to the development of summer eczema.
  • Immediate skin reactions were also observed in five out of the six healthy horses in response to anti-IgE and anti-IgG(T) injections, suggesting that IgG(T) can bind to skin mast cells and might contribute to clinical allergy.

Cite This Article

APA
Wagner B, Miller WH, Morgan EE, Hillegas JM, Erb HN, Leibold W, Antczak DF. (2006). IgE and IgG antibodies in skin allergy of the horse. Vet Res, 37(6), 813-825. https://doi.org/10.1051/vetres:2006039

Publication

Veterinary research
ISSN: 0928-4249
NlmUniqueID: 9309551
Country: England
Language: English
Volume: 37
Issue: 6
Pages: 813-825

Researcher Affiliations

Wagner, Bettina
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. bw73@cornell.edu
Miller, William H
    Morgan, Erin E
      Hillegas, Julia M
        Erb, Hollis N
          Leibold, Wolfgang
            Antczak, Douglas F

              MeSH Terms

              • Allergens / immunology
              • Animals
              • Antibodies / analysis
              • Ceratopogonidae / immunology
              • Eczema / immunology
              • Eczema / veterinary
              • Enzyme-Linked Immunosorbent Assay / veterinary
              • Female
              • Horse Diseases / immunology
              • Horses
              • Hypersensitivity, Immediate / immunology
              • Hypersensitivity, Immediate / veterinary
              • Immunoglobulin E / immunology
              • Immunoglobulin G / immunology
              • Intradermal Tests / veterinary
              • Male

              Citations

              This article has been cited 23 times.
              1. Simonin EM, Torsteinsdóttir S, Svansson V, Björnsdóttir S, Freer H, Tarsillo J, Wagner B. Early allergen introduction overrides allergy predisposition in offspring of horses with Culicoides hypersensitivity. Front Immunol 2025;16:1654693.
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