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Home / Equine Research Bank / Antioxidants (Basel) / Impact of Chlorogenic Acid on Peripheral Blood Mononuclear C...
Antioxidants (Basel, Switzerland)2023; 12(11); 1924; doi: 10.3390/antiox12111924

Impact of Chlorogenic Acid on Peripheral Blood Mononuclear Cell Proliferation, Oxidative Stress, and Inflammatory Responses in Racehorses during Exercise.

Abstract: Green coffee extract is currently of great interest to researchers due to its high concentration of chlorogenic acid (CGA) and its potential health benefits. CGA constitutes 6 to 10% of the dry weight of the extract and, due to its anti-inflammatory properties, is a promising natural supplement and agent with therapeutic applications. The purpose of our study was to discover the effects of CGA on peripheral blood mononuclear cell proliferation, and the production of pro- and anti-inflammatory cytokines as well as reactive oxidative species (ROS) in horses during exercise. According to the findings, CGA can affect the proliferation of T helper cells. In addition, at a dose of 50 g/mL, CGA increased the activation of CD4+FoxP3+ and CD8+FoxP3+ regulatory cells. Physical activity decreases ROS production in CD5+ monocytes, but this effect depends on the concentration of CGA, and the effect of exercise on oxidative stress was lower in CD14+ than in CD5+ cells. Regardless of CGA content, CGA significantly increased the release of the anti-inflammatory cytokine IL-10. Moreover, the production of IL-17 was greater in cells treated with 50 g/mL of CGA from beginners compared to the control and advanced groups of horses. Our findings suggest that CGA may have immune-enhancing properties. This opens new avenues of research into the mechanisms of action of CGA and possible applications in prevention and health promotion in sport animals.
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Publication Date: 2023-10-28 PubMed ID: 38001777PubMed Central: PMC10669817DOI: 10.3390/antiox12111924Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research looks at how chlorogenic acid (CGA), a compound in green coffee extract, affects immune cell function, inflammation, and oxidative stress in racehorses during exercise.

Objective of the Study

  • The aim of the study was to understand the impact of CGA on the proliferation of peripheral blood mononuclear cells, which are essential immune cells, and the production of pro-inflammatory and anti-inflammatory cytokines, which are molecules that regulate immune and inflammatory responses. The researchers also examined the effect of CGA on the production of reactive oxidative species (ROS), which are chemicals that can cause damage to cells, in horses during physical activity.

Methodology and Results

  • The study found that CGA influenced the proliferation of a subset of immune cells, T helper cells.
  • When administered at a dose of 50 g/mL, it was found to increase the activation of specific regulatory cells, CD4+FoxP3+ and CD8+FoxP3+.
  • The research indicates that CGA reduced the production of ROS in CD5+ monocytes (another type of immune cell) during physical activity. However, this effect was dependent on the concentration of CGA.
  • On the other hand, the effect of exercise on oxidative stress was less in CD14+ cells, one type of monocyte, compared to CD5+ cells.
  • CGA was found to significantly boost the release of IL-10, an anti-inflammatory cytokine, regardless of its concentration.
  • Moreover, there was a greater production of IL-17, another cytokine, in cells treated with 50 g/mL of CGA in the beginners’ group of horses compared to the control and advanced groups.

Conclusion and Implications

  • The study provides evidence that CGA might enhance the immune response, indicating potential therapeutic applications for the prevention of diseases and health promotion in sports animals such as racehorses.
  • This understanding could also open new avenues in research to understand the mechanisms through which CGA functions and its potential health benefits.

Cite This Article

APA
Dąbrowska I, Grzędzicka J, Niedzielska A, Witkowska-Piłaszewicz O. (2023). Impact of Chlorogenic Acid on Peripheral Blood Mononuclear Cell Proliferation, Oxidative Stress, and Inflammatory Responses in Racehorses during Exercise. Antioxidants (Basel), 12(11), 1924. https://doi.org/10.3390/antiox12111924

Publication

Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
NlmUniqueID: 101668981
Country: Switzerland
Language: English
Volume: 12
Issue: 11
PII: 1924

Researcher Affiliations

Dąbrowska, Izabela
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Grzędzicka, Jowita
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Niedzielska, Adrianna
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Witkowska-Piłaszewicz, Olga
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.

Grant Funding

  • 2021/41/B/NZ7/03548 / National Science Center

Conflict of Interest Statement

The authors declare no conflict of interest.

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