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Scientific reports2025; 15(1); 43295; doi: 10.1038/s41598-025-03271-6

Impact of dietary essential fatty acids on phospholipid composition and mitochondrial function in aged mares.

Abstract: Advancing age is associated with a decline in fertility and functional capacity, which may result in part from suboptimal nutrition and impaired mitochondrial function. Dietary essential polyunsaturated fatty acids (PUFA) are broadly recommended to mitigate weight loss and reduce risk of chronic disease in aged populations, but their effects on mitochondrial function are less clear. The present study investigated the impacts of dietary supplementation with essential omega-3 PUFA (flaxseed oil; N3) or omega-6 PUFA (corn oil, N6) on blood, muscle and follicular cell fatty acid composition and mitochondrial function in aged light-horse mares (23.2 ± 1.1 year), which are excellent models of human reproductive aging and a focus of dietary interventions in the equine industry. Diets were fed for 6 weeks before adding a supplemental supportive nutrient formulation (DS) designed to optimize equine metabolic health for another 6 weeks. Results demonstrate tissue-specific effects of the dietary oils on mitochondrial function, most notably a decrease in oxidative capacity of platelets and muscle, and greater release of reactive oxygen species (ROS) from granulosa cells and muscle, with no significant difference in effects between N3 and N6 diets. Addition of the DS improved oxidative phosphorylation efficiency and tended to reduce ROS release across all cell types, and increased basal oocyte oxygen consumption. Taken together, these results provide novel insight to the diverse impacts of dietary PUFA intake on equine mitochondrial function, and suggest that supportive nutrients may be required to prevent negative health effects of dietary oil supplementation in aged mares.
© 2025. The Author(s).
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Publication Date: 2025-12-05 PubMed ID: 41350304PubMed Central: PMC12686463DOI: 10.1038/s41598-025-03271-6Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

Overview

  • This study examines how dietary supplementation with essential fatty acids, specifically omega-3 and omega-6 polyunsaturated fatty acids (PUFAs), affects phospholipid composition and mitochondrial function in aged mares.
  • The research evaluates changes in blood, muscle, and follicular cells, and assesses whether a supportive nutrient formulation can improve mitochondrial health and reduce oxidative stress in these animals.

Background and Rationale

  • Aging in mares, similar to humans, is linked to decreased fertility and reduced physical function.
  • One potential cause of decline in function is mitochondrial dysfunction, which affects cells’ energy production and leads to increased oxidative stress.
  • Essential PUFAs (omega-3 and omega-6) are often recommended to support health in aged populations, but their specific effects on mitochondria are not well understood.
  • Aged mares are a valuable model for studying reproductive aging and nutritional interventions relevant to both animal and potentially human health.

Study Design and Methods

  • Subjects: Aged light-horse mares averaging 23.2 years old.
  • Dietary Intervention:
    • Six weeks on either omega-3 PUFA supplement (flaxseed oil, N3) or omega-6 PUFA supplement (corn oil, N6).
    • Followed by six weeks with the addition of a supportive nutrient formulation (DS) designed to optimize equine metabolic health.
  • Tissue Sampling: Blood platelets, muscle tissue, and follicular cells were analyzed.
  • Measurements:
    • Fatty acid composition in phospholipids.
    • Mitochondrial function including oxidative capacity and oxidative phosphorylation efficiency.
    • Generation of reactive oxygen species (ROS), indicators of oxidative stress.
    • Basal oxygen consumption rates, especially in oocytes.

Key Findings

  • Tissue-Specific Effects:
    • Both N3 and N6 diets decreased mitochondrial oxidative capacity in platelets and muscle tissue.
    • There was an increase in ROS production from granulosa cells and muscle, indicating higher oxidative stress.
    • No significant differences were observed between omega-3 and omega-6 supplementation regarding these mitochondrial changes.
  • Impact of Supportive Nutrient Formulation (DS):
    • Improved the efficiency of oxidative phosphorylation across examined cell types, suggesting better mitochondrial energy production.
    • Tended to reduce ROS release, pointing to decreased oxidative stress.
    • Increased basal oxygen consumption specifically in oocytes, implying enhanced cellular metabolic activity relevant to fertility.

Interpretation and Implications

  • Dietary supplementation with essential fatty acids alone may impair mitochondrial function and increase oxidative stress in aged mares, regardless of the omega-3 or omega-6 source.
  • The addition of targeted supportive nutrients appears to mitigate these negative effects by boosting mitochondrial efficiency and lowering oxidative damage.
  • This suggests that essential PUFA supplementation in aged populations may require concurrent nutrient support to avoid adverse effects on cellular metabolism.
  • The findings provide valuable insights for managing reproductive and metabolic aging in mares and potentially guide nutritional strategies in other aging animals and humans.

Conclusions

  • The study reveals the complex role of dietary essential fatty acids on mitochondrial health in aged mares.
  • Supportive nutrient formulations can offset some negative consequences of PUFA supplementation, improving mitochondrial function and reducing oxidative stress.
  • These results emphasize the need to consider combined nutritional strategies rather than isolated supplementation for maintaining health in aging individuals.

Cite This Article

APA
Fresa K, Catandi GD, Gonzalez-Castro R, Omar A, Whitcomb LA, Cheng MH, Chen TW, Carnevale EM, Chicco AJ. (2025). Impact of dietary essential fatty acids on phospholipid composition and mitochondrial function in aged mares. Sci Rep, 15(1), 43295. https://doi.org/10.1038/s41598-025-03271-6

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 15
Issue: 1
Pages: 43295
PII: 43295

Researcher Affiliations

Fresa, Kyle
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
Catandi, Giovana D
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
  • Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK, 74078, USA.
Gonzalez-Castro, Raul
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
Omar, Asma
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
Whitcomb, Luke A
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
Cheng, Ming-Hao
  • Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO, 80523, USA.
Chen, Thomas W
  • Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO, 80523, USA.
Carnevale, Elaine M
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA.
Chicco, Adam J
  • Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523-1680, USA. Adam.chicco@colostate.edu.

MeSH Terms

  • Animals
  • Female
  • Horses
  • Mitochondria / metabolism
  • Mitochondria / drug effects
  • Dietary Supplements
  • Phospholipids / metabolism
  • Aging / metabolism
  • Fatty Acids, Essential / pharmacology
  • Fatty Acids, Essential / administration & dosage
  • Fatty Acids, Omega-6 / administration & dosage
  • Reactive Oxygen Species / metabolism
  • Fatty Acids, Omega-3 / administration & dosage
  • Animal Feed
  • Diet

Conflict of Interest Statement

Declarations. Competing interests: The authors declare no competing interests.

References

This article includes 61 references
  1. Larsson L. Aging-Related loss of muscle mass and function.. (1), 427–511 (2019).
    doi: 10.1152/physrev.00061.2017pmc: PMC6442923pubmed: 30427277google scholar: lookup
  2. Dent E, Wright ORL, Woo J, Hoogendijk EO. Malnutrition in older adults.. (10380), 951–966 (2023).
    doi: 10.1016/S0140-6736(22)02612-5pubmed: 36716756google scholar: lookup
  3. Kim J. Age-Related changes in metabolic properties of equine skeletal muscle associated with muscle plasticity.. (3), 397–403 (2005).
    doi: 10.1016/j.tvjl.2004.03.016pubmed: 15848782google scholar: lookup
  4. Ireland JL. Management, preventive health care and disease in aged horses.. (2), 195–214 (2016).
    doi: 10.1016/j.cveq.2016.04.001pubmed: 27449388google scholar: lookup
  5. Rizzo M. The horse as a natural model to study reproductive Aging-Induced aneuploidy and weakened centromeric cohesion in oocytes.. (21), 22220–22232 (2020).
    doi: 10.18632/aging.104159pmc: PMC7695376pubmed: 33139583google scholar: lookup
  6. Jarvis NG. Nutrition of the aged horse.. (1), 155–166 (2009).
    doi: 10.1016/j.cveq.2009.01.003pubmed: 19303557google scholar: lookup
  7. Siciliano PD. Nutrition and feeding of the geriatric horse.. (3), 491–508 (2002).
    doi: 10.1016/S0749-0739(02)00028-7pubmed: 12516930google scholar: lookup
  8. Mozaffarian D, Micha R, Wallace S. Effects on coronary heart disease of increasing polyunsaturated fat in place of saturated fat: A systematic review and Meta-Analysis of randomized controlled trials.. (3), e1000252 (2010).
    doi: 10.1371/journal.pmed.1000252pmc: PMC2843598pubmed: 20351774google scholar: lookup
  9. Tortosa-Caparrós E, Navas-Carrillo D, Marín F, Orenes-Piñero E. Anti-Inflammatory effects of Omega 3 and Omega 6 polyunsaturated fatty acids in cardiovascular disease and metabolic syndrome.. (16), 3421–3429 (2017).
    doi: 10.1080/10408398.2015.1126549pubmed: 26745681google scholar: lookup
  10. O’Connor CI. The effect of dietary fish oil supplementation on exercising horses.. (10), 2978–2984 (2004).
    doi: 10.2527/2004.82102978xpubmed: 15484950google scholar: lookup
  11. Simopoulos AP. Human requirement for N-3 polyunsaturated fatty acids.. (7), 961–970 (2000).
    doi: 10.1093/ps/79.7.961pubmed: 10901194google scholar: lookup
  12. Clauss M, Codron D, Hummel J. Equid nutritional physiology and behavior: an evolutionary perspective.. , 104265 (2023).
    doi: 10.1016/j.jevs.2023.104265pubmed: 36893821google scholar: lookup
  13. Chilton F. Diet-Gene interactions and PUFA metabolism: A potential contributor to health disparities and human diseases.. (5), 1993–2022 (2014).
    doi: 10.3390/nu6051993pmc: PMC4042578pubmed: 24853887google scholar: lookup
  14. Ishihara T, Yoshida M, Arita M. Omega-3 fatty Acid-Derived mediators that control inflammation and tissue homeostasis.. (9), 559–567 (2019).
    doi: 10.1093/intimm/dxz001pubmed: 30772915google scholar: lookup
  15. Kris-Etherton P, Fleming J, Harris WS. The debate about N-6 polyunsaturated fatty acid recommendations for cardiovascular health.. (2), 201–204 (2010).
    doi: 10.1016/j.jada.2009.12.006pubmed: 20102846google scholar: lookup
  16. Sherratt SCR, Libby P, Budoff MJ, Bhatt DL, Mason RP. Role of Omega-3 fatty acids in cardiovascular disease: the debate continues.. (1), 1–17 (2023).
    doi: 10.1007/s11883-022-01075-xpmc: PMC9834373pubmed: 36580204google scholar: lookup
  17. Li C, White SH, Warren LK, Wohlgemuth SE. Effects of aging on mitochondrial function in skeletal muscle of American American quarter horses.. (1), 299–311 (2016).
    doi: 10.1152/japplphysiol.01077.2015pmc: PMC5040552pubmed: 27283918google scholar: lookup
  18. Catandi G. Effects of maternal age on oxygen consumption of oocytes and in Vitro-Produced equine embryos.. (2), 175–175 (2020).
    doi: 10.1071/RDv32n2Ab98google scholar: lookup
  19. Catandi GD. Equine maternal aging affects oocyte lipid content, metabolic function and developmental potential.. (4), 399–409 (2021).
    doi: 10.1530/REP-20-0494pmc: PMC7969451pubmed: 33539317google scholar: lookup
  20. Pasquariello R. Alterations in oocyte mitochondrial number and function are related to spindle defects and occur with maternal aging in mice and humans†.. (4), 971–981 (2019).
    doi: 10.1093/biolre/ioy248pubmed: 30476005google scholar: lookup
  21. Grevendonk L. Impact of aging and exercise on skeletal muscle mitochondrial capacity, energy metabolism, and physical function.. (1), 4773 (2021).
    doi: 10.1038/s41467-021-24956-2pmc: PMC8346468pubmed: 34362885google scholar: lookup
  22. Catandi GD. Oocyte metabolic function, lipid composition, and developmental potential are altered by diet in older mares.. (4), 183–198 (2022).
    doi: 10.1530/REP-21-0351pmc: PMC8942336pubmed: 37379450google scholar: lookup
  23. Aleksandrova K, Koelman L, Rodrigues CE. Dietary patterns and biomarkers of oxidative stress and inflammation: A systematic review of observational and intervention studies.. , 101869 (2021).
    doi: 10.1016/j.redox.2021.101869pmc: PMC8113044pubmed: 33541846google scholar: lookup
  24. Fritsche KL, Johnston PV. Rapid autoxidation of fish oil in diets without added antioxidants.. (4), 425–426 (1988).
    doi: 10.1093/jn/118.4.425pubmed: 3357057google scholar: lookup
  25. Henneke DR, Potter GD, Kreider JL, Yeates BF. Relationship between condition score, physical measurements and body fat percentage in mares.. (4), 371–372 (1983).
    pubmed: 6641685doi: 10.1111/j.2042-3306.1983.tb01826.xgoogle scholar: lookup
  26. Carter RA, Geor RJ, Burton Staniar W, Cubitt TA, Harris PA. Apparent adiposity assessed by standardised scoring systems and morphometric measurements in horses and ponies.. (2), 204–210 (2009).
    doi: 10.1016/j.tvjl.2008.02.029pubmed: 18440844google scholar: lookup
  27. . Isolation of Human Platelets from Whole Blood. .
  28. Carnevale EM. Advances in collection, transport and maturation of equine oocytes for assisted reproductive techniques.. (3), 379–399 (2016).
    doi: 10.1016/j.cveq.2016.07.002pubmed: 27726987google scholar: lookup
  29. Wagner B, Freer H. Development of a Bead-Based multiplex assay for simultaneous quantification of cytokines in horses.. (3–4), 242–248 (2009).
    doi: 10.1016/j.vetimm.2008.10.313pubmed: 19027964google scholar: lookup
  30. Mulligan CM, Le CH, deMooy AB, Nelson CB, Chicco AJ. Inhibition of Delta-6 desaturase reverses Cardiolipin remodeling and prevents contractile dysfunction in the aged mouse heart without altering mitochondrial respiratory function.. (7), 799–809 (2014).
    doi: 10.1093/gerona/glt209pmc: PMC4111632pubmed: 24418793google scholar: lookup
  31. Li Puma LC. Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. (5), R972–R980 (2020).
    doi: 10.1152/ajpregu.00227.2019pubmed: 32233925google scholar: lookup
  32. Doerrier C. High-Resolution fluorespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Mitochondrial Bioenergetics: Methods and Protocols Springer New York: New York, NY; 31–70 (2018).
    pubmed: 29850993doi: 10.1007/978-1-4939-7831-1_3google scholar: lookup
  33. Pesta D, Gnaiger E. High-Resolution Respirometry OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. 25–58 (2011).
    doi: 10.1007/978-1-61779-382-0_3pubmed: 22057559google scholar: lookup
  34. Starkov AA. Measurement of mitochondrial ROS production. Methods in Molecular Biology Vol. 648. pp 245–255 (Humana Press, 2010).
    pmc: PMC3057530pubmed: 20700717doi: 10.1007/978-1-60761-756-3_16google scholar: lookup
  35. Obeidat YM. Design of a Multi-Sensor platform for integrating extracellular acidification rate with Multi-Metabolite flux measurement for small biological samples. 39–47 (2019).
    doi: 10.1016/j.bios.2019.02.069pmc: PMC6660976pubmed: 30909011google scholar: lookup
  36. Cheng MH, Chicco AJ, Ball D, Chen TW. Analysis of mitochondrial oxygen consumption and hydrogen peroxide release from cardiac mitochondria using electrochemical Multi-Sensors. 131641 (2022).
    doi: 10.1016/j.snb.2022.131641google scholar: lookup
  37. Bedard K, Krause KH. The NOX family of ROS-Generating NADPH oxidases: physiology and pathophysiology. (1), 245–313 (2007).
    doi: 10.1152/physrev.00044.2005pubmed: 17237347google scholar: lookup
  38. Mowry KC. Effects of crude rice Bran oil and a flaxseed oil blend in young horses engaged in a training program. (21) (2022).
    pmc: PMC9653641pubmed: 36359130doi: 10.3390/ani12213006google scholar: lookup
  39. Williams CM, Burdge G. Long-chain – 3 PUFA: Plant v. Marine sources. (1), 42–50 (2006).
    pubmed: 16441943doi: 10.1079/pns2005473google scholar: lookup
  40. Pearson G, Goodale M, Wakshlag J, Fortier L. Dose-dependent increase in whole blood Omega-3 fatty acid concentration in horses receiving a marine-based fatty-acid supplement. .
    pubmed: 34800796doi: 10.1016/j.jevs.2021.103781google scholar: lookup
  41. Christmann U. Dynamics of DHA and EPA supplementation: Incorporation into equine plasma, synovial fluid, and surfactant glycerophosphocholines. (5) (2021).
    pubmed: 33866431doi: 10.1007/s11306-021-01792-5google scholar: lookup
  42. Schwärzler J. PUFA-Induced metabolic enteritis as a fuel for Crohn’s disease. (6), 1690–1704 (2022).
    doi: 10.1053/j.gastro.2022.01.004pubmed: 35031299google scholar: lookup
  43. Zhang Y, Mahmood T, Tang Z, Wu Y, Yuan J. Effects of naturally oxidized corn oil on inflammatory reaction and intestinal health of broilers. (1), 101541 (2022).
    doi: 10.1016/j.psj.2021.101541pmc: PMC8605181pubmed: 34788712google scholar: lookup
  44. Rohrbach S. Effects of dietary polyunsaturated fatty acids on mitochondria. (36), 4103–4116 (2009).
    doi: 10.2174/138161209789909692pubmed: 20041812google scholar: lookup
  45. Jedlička J, Kunc R, Kuncová J. Mitochondrial respiration of human platelets in young adult and advanced age – Seahorse or O2k?. S369–S379 (2021).
    pmc: PMC8884395pubmed: 35099255doi: 10.33549/physiolres.934812google scholar: lookup
  46. Braganza A. Platelet bioenergetics correlate with muscle energetics and are altered in older adults. (13), e128248 (2019).
    doi: 10.1172/jci.insight.128248pmc: PMC6629251pubmed: 31120438google scholar: lookup
  47. Mulligan CM. Dietary linoleate preserves Cardiolipin and attenuates mitochondrial dysfunction in the failing rat heart. (3), 460–468 (2012).
    doi: 10.1093/cvr/cvs118pmc: PMC3353802pubmed: 22411972google scholar: lookup
  48. Herbst EAF. Omega-3 supplementation alters mitochondrial membrane composition and respiration kinetics in human skeletal muscle. (6), 1341–1352 (2014).
    doi: 10.1113/jphysiol.2013.267336pmc: PMC3961091pubmed: 24396061google scholar: lookup
  49. Varming K. The effect of N-3 fatty acids on neutrophil chemiluminescence. (1), 47–52 (1995).
    doi: 10.3109/00365519509075377pubmed: 7624736google scholar: lookup
  50. Schönfeld P, Schlüter T, Fischer KD, Reiser G. Non-Esterified polyunsaturated fatty acids distinctly modulate the mitochondrial and cellular ROS production in normoxia and hypoxia. (1), 69–78 (2011).
    doi: 10.1111/j.1471-4159.2011.07286.xpubmed: 21517851google scholar: lookup
  51. Suzuki N. Association between polyunsaturated fatty acid and reactive oxygen species production of neutrophils in the general population. (11), 3222 (2020).
    doi: 10.3390/nu12113222pmc: PMC7690262pubmed: 33105547google scholar: lookup
  52. Lalia AZ. Influence of Omega-3 fatty acids on skeletal muscle protein metabolism and mitochondrial bioenergetics in older adults. (4), 1096–1129 (2017).
    doi: 10.18632/aging.101210pmc: PMC5425117pubmed: 28379838google scholar: lookup
  53. Martins AR. Attenuation of obesity and insulin resistance by fish oil supplementation is associated with improved skeletal muscle mitochondrial function in mice fed a High-Fat diet. 76–88 (2018).
    doi: 10.1016/j.jnutbio.2017.11.012pubmed: 29413492google scholar: lookup
  54. Cabrero À. Increased reactive oxygen species production Down-Regulates peroxisome Proliferator-Activated α pathway in C2C12 skeletal muscle cells. (12), 10100–10107 (2002).
    doi: 10.1074/jbc.M110321200pubmed: 11792699google scholar: lookup
  55. Ferreira LF, Laitano O. Regulation of NADPH oxidases in skeletal muscle. 18–28 (2016).
    doi: 10.1016/j.freeradbiomed.2016.05.011pmc: PMC4975970pubmed: 27184955google scholar: lookup
  56. Basuroy S, Bhattacharya S, Leffler CW, Parfenova H. Nox4 NADPH oxidase mediates oxidative stress and apoptosis caused by TNF-α in cerebral vascular endothelial cells. (3), C422–C432 (2009).
    doi: 10.1152/ajpcell.00381.2008pmc: PMC2660262pubmed: 19118162google scholar: lookup
  57. White-Springer SH, Vineyard KR, Kivipelto J, Warren LK. Dietary Omega-3 fatty acid supplementation does not impair vitamin E status or promote lipid peroxidation in growing horses. (7), skab177 (2021).
    doi: 10.1093/jas/skab177pmc: PMC8259830pubmed: 34228797google scholar: lookup
  58. Karuputhula NB, Chattopadhyay R, Chakravarty B, Chaudhury K. Oxidative status in granulosa cells of infertile women undergoing IVF. (2), 91–98 (2013).
    doi: 10.3109/19396368.2012.743197pubmed: 23278116google scholar: lookup
  59. Gu L. Metabolic control of oocyte development: linking maternal nutrition and reproductive outcomes. (2), 251–271 (2015).
    doi: 10.1007/s00018-014-1739-4pmc: PMC4389777pubmed: 25280482google scholar: lookup
  60. Latham CM, Dickson EC, Owen RN, Larson CK, White-Springer SH. Complexed trace mineral supplementation alters antioxidant activities and expression in response to trailer stress in yearling horses in training. (1), 7352 (2021).
    doi: 10.1038/s41598-021-86478-7pmc: PMC8016935pubmed: 33795725google scholar: lookup
  61. Owen RN, Semanchik PL, Latham CM, Brennan KM, White-Springer SH. Elevated dietary selenium rescues mitochondrial capacity impairment induced by decreased vitamin E intake in young exercising horses. (8), skac172 (2022).
    doi: 10.1093/jas/skac172pmc: PMC9339289pubmed: 35908793google scholar: lookup

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