Analyze Diet

Topic:Nutrition

Nutrition in horses encompasses the study of dietary requirements and feeding practices that support equine health, growth, and performance. It involves the analysis of nutrients such as carbohydrates, proteins, fats, vitamins, and minerals, and their roles in equine physiology. Proper nutrition is essential for maintaining optimal body condition, supporting metabolic processes, and preventing dietary-related disorders. Research in this field examines the nutritional needs of horses at different life stages and activity levels, as well as the effects of various feed types and supplements. This page compiles peer-reviewed research studies and scholarly articles that explore the composition, digestion, and impact of different dietary components on equine health and performance.
Airway Lipid Mediators in Horses: Baseline Bronchoalveolar Lavage Fluid Profiles and Effects of EPA and DHA-rich Microalgae Oil Supplementation.
Veterinary journal (London, England : 1997)    August 28, 2026   106862 doi: 10.1016/j.tvjl.2026.106862
Vainio Kati ME, House Andrew H, Höglund Nina M, Hanna R, Kurkela Kaisla MM, Leino Aino E, Reijo K, Mykkänen Anna K.We investigated how dietary omega-3 polyunsaturated fatty acids (n-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) affect airway inflammation, bronchoalveolar lavage fluid (BALF) lipid mediators and red blood cell fatty acids in horses. Increased BALF neutrophils are a hallmark of airway inflammation, commonly associated with equine asthma. The long-chain n-3 PUFAs EPA and DHA are essential nutrients and precursors of specialized pro-resolving mediators (SPMs) that regulate inflammatory responses. In this prospective field trial, we assigned horses to three diets, including...
Effect of sample storage on pH-value in equine faeces.
Translational animal science    August 25, 2026   Volume 10 txag131 doi: 10.1093/tas/txag131
Lindroth KM, Müller CE.Measurements of pH-value in equine faeces are often performed in studies on nutrition and health in horses, but a standard protocol for it is lacking despite the knowledge that different sample handling may affect the pH-value. Sample handling prior to pH-measurement is often determined by practical settings and has been reported to vary in published studies within the area. The purpose of the current study was therefore to investigate the influence of different combinations of storage time and temperature on the pH-value in equine faecal samples in comparison to immediate pH-measurement at de...
Correction to: PSI-9 Efficacy of Electrolyte Chews in Horses.
Journal of animal science    August 20, 2026   Volume 104 skag241 doi: 10.1093/jas/skag241
No abstract available
Relationship Between Feed Olfactory Exploration and Consumption in Horses.
Animals : an open access journal from MDPI    August 20, 2026   Volume 16, Issue 16 2599 doi: 10.3390/ani16162599
Wnuk E, Janicka W, Łuszczyński J, Wojtyniak A, Jagusiak W, Stachurska A.The aim of the study was to evaluate the relationship between horses' behaviour during olfactory exploration and following consumption of the same food containing herbs, either previously exposed to the herb odour or not. Twenty horses' responses to 14 herbs were compared in a smelling test, when the horses sniffed a herb, and after 20 min a consumption test, when they consumed oats mixed with the same herb. Before the tests, the horses were exposed to the scent of seven herbs, whereas the remaining seven herbs were non-pre-exposed. The results show that horses consumed oats with herbal additi...
From Diet to Disease: The Role of the Gut Microbiome and Microbial Metabolites in Horses.
Veterinary sciences    August 18, 2026   Volume 13, Issue 8 823 doi: 10.3390/vetsci13080823
Wu Y, Ma Y, Zan Y, Bai X, Li J, Li H.The equine gastrointestinal microbiome plays an essential role in digestion, energy metabolism, immune regulation, and maintenance of intestinal homeostasis. Increasing evidence suggests that microbial-derived metabolites provide a functional link between diet, the microbiome, and host physiology. This narrative review summarizes current knowledge on how dietary factors, including structural carbohydrates, non-structural carbohydrates, protein, and lipids, influence microbial function and metabolite production in horses. Relevant publications available up to June 2026 were identified through s...
Correlation between microbial communities, metabolites, and bioactivities in fermented mare’s milk revealed by metagenomics and metabolomics.
Food microbiology    August 17, 2026   Volume 141 105278 doi: 10.1016/j.fm.2026.105278
Huang X, Luo R, Wang Y, Zheng W, Lu X, Liu G, Abdulla P, Niu R, Tu Y, Xing J, Hong J, Zheng W, Liu J.Koumiss exhibits various functional properties, including antioxidant, hypoglycemic, and antihypertensive effects; however, natural fermentation is characterized by an uncontrollable microbial community structure, significant fluctuations in product quality, and a lack of specialized fermentation agents. The mechanisms underlying the links between microbial communities, functional metabolites, and bioactivity in natural and inoculated fermentation systems remain unclear. Therefore, this study employed bioactivity assays, metagenomics, and non-targeted metabolomics to systematically analyze dif...
Administration of the Postbiotic Substance 412 to Slovak Warm-Blooded Horses: A Pilot Experiment.
Life (Basel, Switzerland)    August 16, 2026   Volume 16, Issue 8 1342 doi: 10.3390/life16081342
Lauková A, Focková V, Micenková L, Gancarčíková S, Plachá I, Grešáková Ľ, Kováčik A, Halo M, Gálik B, Pogány Simonová M.Antimicrobial proteinaceous postbiotic substances (PS) and/or bacteriocins can beneficially influence animal health, as previously shown with enterocins from non-autochthonous enterococcal strains. Therefore, the effectiveness of PS 412, produced by our beneficial autochthonous strain EF 412, was assessed in horses. In the pilot experiment, PS 412 was administered to 12 horses of various ages and breeds for 21 days via a small feed ball. Sampling was performed on days 0/1, 21, and 42 (after a 3-week cessation of PS 412). The horses served as their own controls. Although the number of horses w...
The effects of diet on the physical characteristics of feces and fiber digestibility in healthy horses.
Journal of animal science    August 13, 2026   skag255 doi: 10.1093/jas/skag255
Starrett A, Sarturi JO, Leatherwood JL, Chaffin MK, Mukhopadhyay S, Pilla R, Arnold CE.Diet is a known risk factor for colic, but few studies have explored how diet affects fecal characteristics which are often altered in horses with colic. This prospective cross-over study evaluated the effects of two common equine diets on fecal characteristics and fiber digestibility in healthy adult horses. We hypothesized that horses offered a complete pelleted diet would exhibit increased fecal dry matter (DM) and decreased fecal particle size compared to those offered a forage-based diet. Twenty horses at Texas Tech University School of Veterinary Medicine served as subjects in the study....
Energy Metabolism Dysregulation in Horses and Foals.
The Veterinary clinics of North America. Equine practice    August 12, 2026   S0749-0739(26)00052-0 doi: 10.1016/j.cveq.2026.07.005
Hostnik LD.Dysregulation of energy metabolism occurs in horses and foals with transient systemic illness, malnutrition, or chronic metabolic diseases. The pathophysiologic processes resulting in energy dysregulation are important to interpret, understand, and appropriately monitor laboratory abnormalities associated with various disease states (hypoglycemia and hyperglycemia), as well as to aid in the management of energy dysregulation through nutritional and medical management.
Efficiency of a Combination of Polyethylene Glycol and Psyllium on the Evacuation of Sand Compared to Established Treatment Regimes in Sand Enteropathy of the Horse.
Animals : an open access journal from MDPI    August 12, 2026   Volume 16, Issue 16 2507 doi: 10.3390/ani16162507
Lousberg LK, Merle R, Barton AK.The accumulation of sand in the abdomen is a rising risk for colic in horses. Sand mainly accumulates in the ascending colon in the cranioventral abdomen. Evacuation of sand is facilitated by the application of psyllium supported with a laxative via nasogastric intubation. Laxatives currently used are either sodium sulfate/magnesium sulfate or mineral oil. The aim of this study was to compare the efficiency of polyethylene glycol, a laxative already used in human medicine for chronic constipation, with that of the above-described, established therapy options. Therefore, 44 horses diagnosed wit...
Four Haylages, One Metabonome: Defining Diet Driven Metabolic Shifts in Ponies.
Journal of animal physiology and animal nutrition    August 6, 2026   doi: 10.1111/jpn.70099
Moore-Colyer M, Harris P, Daniels S.Haylage is widely fed to all categories of stabled horses across Europe and New Zealand. Haylage can vary from high energy nutrient dense forage to low energy highly fibrous fodder, yet little is known about the impact different haylages have on metabolism in horses. This study provides the first direct comparison of feeding four different haylages: early cut (Lolium multiflorum), later cut (Lolium perenne) rye grass (R1 and R2); early and late cut meadow grass (M1 and M2), on urinary and faecal metabonomics, in ponies at maintenance. Using replicated Latin Square design, eight individually ho...
Growth Performance, Milk Productivity, Productive Longevity, and Milk Composition of Mugalzhar Horses.
Animals : an open access journal from MDPI    July 22, 2026   Volume 16, Issue 14 2272 doi: 10.3390/ani16142272
Toishimanov M, Alikhanov O, Aubakirov K, Karibayeva D, Kargaeva M, Sapakhova Z, Begaliyeva D, Kanat R, Tugunova Y, Daurov D, Daurova A, Zhambakin K....The Mugalzhar horse is an important native breed of Kazakhstan, valued for its adaptability, milk production, and meat productivity under extensive pasture-based management. This study evaluated the relationships among first-lactation milk yield, productive longevity, lifetime milk production, growth, morphometric traits, and milk composition in the Mugalzhar horse breed using data from 100 mares with complete lifetime production records, 108 adult mares and 12 stallions for morphometric evaluation, and 68 foals (35 colts and 33 fillies) for growth analysis. The study was conducted on Mugalzha...
Differential shaping of equine gut microbiota structure and function by breed and feeding regimen.
Frontiers in microbiology    July 16, 2026   Volume 17 1852554 doi: 10.3389/fmicb.2026.1852554
Lin Y, Qiri G, Du M, Dugarjaviin M, Cao J, Fang X, Yun S, Li X, Gong W, Ding W, Bou T, Su S, Chen J, Xing N, Bai D, Zhao Y.The gut microbiota plays an essential role in host energy metabolism and immune function. Horses are non-ruminant herbivores that rely heavily on hindgut microbial fermentation to meet their energy requirements. However, the relative contributions of host genetic background (breed) and environmental factors (feeding regimen and geographical location) to shaping the equine gut microbiota remain poorly understood. Unassigned: In this study, 16S rRNA gene sequencing and functional prediction analysis were performed on 139 equine fecal samples to systematically investigate the differential effects...
A Comprehensive Review of the Equine Gut Microbiome in Health and Disease.
Veterinary sciences    July 7, 2026   Volume 13, Issue 7 659 doi: 10.3390/vetsci13070659
Ericsson AC.Molecular microbiology has revolutionized our understanding of the complex host-associated microbiomes required for normative development and physiology. Horses and other members of the family are particularly reliant on the early maturation and lifelong maintenance of an unusually rich hindgut microbiome for optimal digestion and overall health and performance. Research on the equine gut microbiome has accelerated in the past several years, necessitating a renewed appraisal of the field. The present work is a comprehensive and critical review of the literature regarding the bacterial gastroi...
Risk factors for post-partum colic in a population of UK and Irish Thoroughbred broodmares.
Equine veterinary journal    June 30, 2026   doi: 10.1002/evj.70219
Cullen E, Carslake HB, Henderson I, Stoneham S, Pinchbeck GL, Archer DC.Broodmares are at increased risk of gastrointestinal colic. However, colic risk factors specific to this population have not been investigated. Objective: To investigate horse- and management-level risk factors for post-partum colic in a cohort of Thoroughbred broodmares in the United Kingdom and Ireland. Methods: Prospective, nested case-control study. Methods: Twenty-nine Thoroughbred stud farms in the United Kingdom and Ireland were recruited, and 1044 mares were followed over the 2021 and/or 2022 foaling seasons. Data were collected on signalment, medical and management history for post-pa...
Short-term provision of moderate dietary starch alters fecal microbiota but does not exacerbate exercise-induced inflammation in yearling Quarter Horses.
Journal of equine veterinary science    June 27, 2026   106071 doi: 10.1016/j.jevs.2026.106071
DiSilvestro AN, Wesolowski LT, Williams BD, Warren LK, Athrey G, White-Springer SH.Energy-dense feeds commonly provided to equine athletes may be high in starch, which alter gastrointestinal microbiota and could promote systemic inflammation. Objective: To test the hypothesis that exercise-induced inflammation would be greater in horses receiving a starch- versus fiber-based concentrate. Methods: Quarter Horses (mean±SD 16±1mo; 337±30kg) received either a fiber-based control (CON; 7 fillies, 8 geldings) or an isocaloric, isonitrogenous starch concentrate (STARCH; 8 fillies, 7 geldings) for 24d. Fecal metagenomics were evaluated on d0 and 21. Blood inflammatory mediators w...
Fecal microbiota of weaned equine and mule foals grazing bermudagrass pastures.
Journal of equine veterinary science    June 27, 2026   106070 doi: 10.1016/j.jevs.2026.106070
Araújo LSA, Franzan BC, Almeida MIV, Coelho IS, Almeida FQ.Weaning is a critical period in the lives of foals. After weaning, forage consumption represents a major challenge for both equine or mule foals. Objective: This study evaluated the fecal bacterial concentration, composition, diversity and differential abundance in weaned equine and mule foals under rotational grazing on Bermudagrass (Cynodon spp.) pasture. Methods: A randomized design was used with equine and mule foals grazing two rotations on three Bermudagrass cultivars (Coastcross, Florakirk, and Tifton-85). Pasture rotation occurred every 20 days. The first rotation was evaluated at 20, ...
Forage nutritive analysis in practical equine feeding: challenges and opportunities.
Journal of equine veterinary science    June 18, 2026   106067 doi: 10.1016/j.jevs.2026.106067
Nilsson ST, Müller CE, Lindqvist H.Previous research has shown that forage nutritive analyses are not used to any large extent in equine nutrition. As forages differ widely in nutritional quality, knowledge of the nutritive value is essential to avoid under- or overfeeding of horses. Objective: The aim of this study was to investigate how forage nutritive analyses were used among horse owners in Sweden, and to identify challenges horse owners encounter with such analyses. Methods: Data was collected through an online survey with questions about the respondent, access to forage analytical reports, if and how the respondent used ...
Placental adaptation to maternal environment in domestic animals.
The Journal of reproduction and development    June 18, 2026   Volume 72, Issue 3 134-142 doi: 10.1262/jrd.2026-006
Chavatte-Palmer P, Dahl GE, Couturier-Tarrade A.The developmental origins of health and disease (DOHaD) is based on the observation that environmental conditions during sensitive windows of development shape lifelong physiology and disease susceptibility. While fetal organs are often viewed as direct programming targets, the placenta is a crucial mechanistic hub because it senses maternal conditions and actively constructs the fetal environment through nutrient transport, endocrine signaling, and immune regulation. Placental plasticity spans morphology and how exchanges are favored by tissue topology, vascular development and perfusion, tra...
Mastication characterization of horses fed bermudagrass hay in a two-part field study.
Journal of equine veterinary science    June 17, 2026   106068 doi: 10.1016/j.jevs.2026.106068
Long JF, Hutton R, Abrams A, Duberstein JA, Duberstein KJ.Bermudagrass (Cynodon dactylon L.) is a common forage in the southeastern United States but remains controversial in equine diets due to reported associations with impaction colic. Reduced fiber digestibility has been proposed as a contributing factor, however differences in mastication of fine-stemmed bermudagrass hay have not been studied and may play a role in digestive disturbances. Objective: To compare chewing characteristics, including chews per unit weight and chews per minute, in horses consuming bermudagrass hay compared to other common hay types. Methods: Two studies were conducted....
Effect of Spirulina platensis extract (C-phycocyanin) supplementation to the semen extender on kinematic and oxidative parameters of Marwari stallions during freezing.
Tropical animal health and production    June 12, 2026   Volume 58, Issue 5 326 doi: 10.1007/s11250-026-05136-0
Yadav V, Talluri TR, Dholpuria S, Khandelwal S, Joshi A.The current research aimed at examining the impact of different quantities of Spirulina platensis extract (C-phycocyanin) supplemented to the semen extender on the post-thaw quality of Marwari stallion spermatozoa. Semen samples were obtained via an artificial vagina from six fertile stallions and diluted with an extender containing Spirulina platensis extract (C-phycocyanin) at concentrations of 10, 20, and 50 µg/mL, or without Spirulina as a control. Aliquots of diluted semen were subjected to cooling (4 °C, 120 min) and then frozen (-196 °C, 1 day), after which the kinematic paramet...
Effect of Antioxidant Supplementation on Systemic Oxidative Status in Breeding Stallions During Intensive Semen Collection.
Animals : an open access journal from MDPI    June 11, 2026   Volume 16, Issue 12 1804 doi: 10.3390/ani16121804
Del Prete C, Ruggiero A, Longobardi C, Cutrignelli MI, Vastolo A.Breeding stallions are subjected to increased metabolic and physiological demands during the reproductive season, which may lead to enhanced production of reactive oxygen species and disruption of redox balance. The present study evaluated the effects of dietary antioxidant supplementation on oxidative status, metabolic profile, and hindgut fermentation in stallions undergoing intensive semen collection activity. Ten adult breeding stallions were assigned either to a control group or to a treatment group receiving a commercial antioxidant supplement (Oxyliver) for 60 days, followed by a 60-day...
Metabolomic Analysis of the Effects of Cysteamine Zinc on the Composition and Amino Acid Profile of Mare’s Milk.
Life (Basel, Switzerland)    June 11, 2026   Volume 16, Issue 6 983 doi: 10.3390/life16060983
Yang F, Ma Y, Li X, Yao X, Yang K, Wang C.This study aims to investigate the effects of cysteamine zinc supplementation on milk production, composition, amino acid profile, and metabolites in mares. Building on prior experimental findings, a dose of 7 mg/kg body weight of CS-Zn was selected for the experimental group, which was compared with a control group. Milk samples were collected at various time points, and milk yield was recorded each time. Routine analysis of milk components, as well as the determination of milk metabolites and amino acids, were performed. The results indicated that, compared to the control group, the experime...
Successful restoration of heathlands and grasslands associated with long-term habitat preferences of cattle and horses: Insights from GPS tracking.
Journal of environmental management    June 9, 2026   Volume 411 130113 doi: 10.1016/j.jenvman.2026.130113
Hendler R, Tischew S, Hensen H, Metze K, Schütz L, Bade K, Fischer C.Long-term, year-round grazing by large herbivores is increasingly used to restore degraded temperate heathlands and grasslands, yet the behavioral mechanisms of grazers underlying restoration remain insufficiently understood. We combined over 13 years of high-resolution GPS-tracking data of free-ranging Heck cattle and Konik horses with repeated vegetation surveys across various habitat types (encroachment-derived Calamagrostis epigejos stands, dry sandy grasslands, dry heaths, and pioneer forests) in Central Germany. We quantified how habitat preference and vegetation structure co-develop und...
Meat Quality Characteristics of Mongolian Horses in Inner Mongolia: Regional Superiority and Transcriptomic Insights into Tenderness Differences Between Muscular Locations.
Animals : an open access journal from MDPI    June 9, 2026   Volume 16, Issue 12 1788 doi: 10.3390/ani16121788
Liu Y, Wang X, Tan G, Dugarjaviina M, Zhang X.Horse meat is a high-quality protein source. A total of 50 two-year-old male horses with similar body weights (300 ± 50 kg) were used: 30 for regional comparison (10 each from Bulgan province region of Mongolia (BPM), Tuv province region of Mongolia (TPM), and Inner Mongolia region of China (IMC)) and an additional 20 from the IMC region for muscle-site analysis. To elucidate the molecular mechanisms underlying regional and muscle-site differences in the meat quality of Mongolian horses, with particular emphasis on tenderness, this study analyzed chemical composition, minerals, fatty acids, a...
Impact of collagen supplementation on the fecal microbiome of young horses in training.
Journal of equine veterinary science    June 6, 2026   106047 doi: 10.1016/j.jevs.2026.106047
Júnior AMCA, da Silva AH, Buroxid RP, Padovan JR, Nunes AT, Poleti MD, Fukumasu H, Gobesso AAO.Intense training in athletic foals may alter fecal microbiota, highlighting the need for nutritional strategies such as hydrolyzed collagen supplementation. Objective: This study aimed to evaluate the effects of dietary hydrolyzed collagen on the fecal microbiome of weanlings in training. Methods: Twenty Mangalarga Marchador foals (206 ± 18 kg; 7 ± 1 mo age) were assigned to two groups: a supplemented group (50 g/day of hydrolyzed collagen for 180 days) and a control group. A completely randomized design with repeated measures over time was used. Foals were exercised five consecutive days pe...
Physiological determinants of plasma biochemical parameters in horses: a structured review.
Journal of equine veterinary science    June 5, 2026   106052 doi: 10.1016/j.jevs.2026.106052
Satué K, La Fauci D, Velasco-Martínez MG, Cravana C, Medica P, Fazio E.The biochemical profile of horses is a cornerstone in equine clinical diagnostics, offering valuable insights into health status, disease progression, and physiological adaptation to internal and external stimuli. However, the interpretation of these biochemical parameters is far from straightforward, as they are subject to significant variation influenced by a range of physiological factors. Age, sex, breed, level of physical activity, nutritional condition, and reproductive status can all modulate baseline biochemical values, potentially leading to diagnostic inaccuracies if not properly con...
Comparative Analysis of Meat Quality and Flavor Among Four Categories of Mongolian Horses.
Foods (Basel, Switzerland)    June 5, 2026   Volume 15, Issue 11 2044 doi: 10.3390/foods15112044
Liu Y, Wang X, Gong S, Dugarjaviina M, Zhang X.This study aims to conduct a comparative analysis of the quality and flavor of meat from four categories of Mongolian horses (Wushen, Baicha, Barhu, and Ujimqin). Physicochemical indicators, electronic nose, electronic tongue, and lipidomics were used to characterize meat quality and flavor and to screen for differential markers. Results showed that Wushen Horses had the highest pH, serine, glutamic acid, total free amino acids (∑FAA), total non-essential amino acids (∑NEAA), total amino acids (∑TAA), NEAA/TAA, W2S sensor response, umami and richness values, and had the lowest cooking lo...
Estimating the economic value of working donkeys in Ethiopia.
Preventive veterinary medicine    May 30, 2026   Volume 254 106926 doi: 10.1016/j.prevetmed.2026.106926
Asteraye GB, Jobling R, Jemberu WT, Pinchbeck G, Knight-Jones TJD, Chaters GL, Rushton J.There is limited empirical evidence on the quantitative economic contribution of donkeys to livelihoods, leading to their undervaluation and exclusion from policy. Here an enterprise budget analysis is embedded within a stochastic dynamic population model for three production systems in Ethiopia to evaluate system inputs, outputs and the economic contribution of donkeys. The model was parameterised using data from household surveys, focus group discussions and key informant interviews, supplemented by literature. The economic value of donkeys' (unpaid) homestead and agricultural services was e...
Recent Advances in Natural Products for Colitis: Mechanisms and Translational Perspectives.
Veterinary sciences    May 29, 2026   Volume 13, Issue 6 532 doi: 10.3390/vetsci13060532
Jin F, Shi Y, Wang L, Kuang T, Ren K, Xu J, Zhang Y, Niu C, Cheng J, Pan S.Colitis is a chronic relapsing inflammatory disorder characterized by persistent mucosal inflammation, epithelial barrier disruption, oxidative stress, immune dysregulation, and gut microbiota imbalance. Although current therapies have improved disease management, their long-term use is still limited by incomplete response, relapse, adverse effects, and high cost. Natural products have attracted increasing attention as promising candidates for colitis intervention because of their structural diversity, broad bioactivity, and multitarget regulatory potential. In veterinary medicine, colitis and...
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