Analyze Diet

PloS one.

Periodical
Medicine
Science
Publisher:
Public Library of Science
Frequency: Monthly
Country: United States
Language: English
Author(s):
Public Library of Science.
Start Year:2006 -
Identifiers
ISSN:1932-6203 (Electronic)
1932-6203 (Linking)
NLM ID:101285081
(OCoLC):70662135
LCCN:2006214532
Correction: Development of an indirect ELISA for the detection of venezuelan equine encephalitis virus specific antibodies in horses.
PloS one    September 15, 2026   Volume 21, Issue 9 e0358506 doi: 10.1371/journal.pone.0358506
[This corrects the article DOI: 10.1371/journal.pone.0338819.].
Improving sport horse selection using machine learning: Early prediction of elite-level show jumping performance.
PloS one    September 15, 2026   Volume 21, Issue 9 e0357799 doi: 10.1371/journal.pone.0357799
Zanchi M, Bordin C, Ablondi M, Asti V, Summer A, Valle E, Ozella L.Early identification of sport potential in show jumping horses remains challenging because competition outcomes vary widely, and elite-level performance develops after considerable time. In this study, we evaluated the applicability of machine learning approaches to competition records from horses identified through FEI participation, complemented by national-level records from Sweden, Belgium and France, with the aim of identifying horses likely to reach elite performance. Performance data from Fédération Équestre Internationale (FEI) competitions were analysed using an Extreme Gradient Bo...
Evidence-based interpretation of uterine cultures in mares: Linking bacterial growth patterns to endometrial inflammation.
PloS one    August 25, 2026   Volume 21, Issue 8 e0356822 doi: 10.1371/journal.pone.0356822
Yáñez U, Krupa M, Gibbons J, Storme J, Lewis N, Pascottini OB.We evaluated uterine bacterial growth patterns and their association with endometrial inflammation (EI) in equine samples collected using uterine swabs (US) or low-volume uterine lavage (UL). A database including 1,545 US and 2,066 UL with cytological and bacteriological results was retrospectively analysed. Endometrial inflammation was defined as ≥2 polymorphonuclear cells per high-power field, and bacterial culture was considered positive when aerobic growth occurred within 48 h, with the number of bacterial isolates per sample recorded (0, 1, 2, or ≥3). Data were analysed using generali...
Transforming retraining: Supporting racehorses to have successful second careers.
PloS one    August 19, 2026   Volume 21, Issue 8 e0355509 doi: 10.1371/journal.pone.0355509
Williams JM, Jordan S, Friend L, Kay E, Edmunds M, Nankervis K, Cheung J, Tabor G.Optimizing long-term welfare outcomes for Thoroughbred racehorses following retirement from racing is a priority across the global horse racing industry. Following their racing career, horses are required to adapt to a markedly different lifestyle, involving substantial physical, behavioural, and mental adjustment. This study consulted experienced retrainers to identify factors that inform successful retraining and transition of former racehorses to promote positive lifelong outcomes beyond racing. An online survey of 100 global stakeholders experienced in retraining former racehorses explor...
Recognition of unfamiliar predators in domestic horses through only visual predator cues.
PloS one    July 15, 2026   Volume 21, Issue 7 e0349298 doi: 10.1371/journal.pone.0349298
Hofacker R, Sebunia N, Pihlblad J, Benderlioglu Z.Acoustic, olfactory, and visual predator cues trigger various adaptive responses among a variety of prey species even when they are under human protection since birth. Previous studies have found that domesticated animals show increased vigilance, stress, flight, and aggregation behaviors in response to predation threats. These investigations, however, did not directly test visual threatening cues alone in animals that communicate more with body language than vocalizations while considering individual temperament and social status. The current study aims to address this issue. Eighteen horses ...
Determining optimal practices for foal weaning – A protocol for a systematic review and network meta-analysis.
PloS one    July 1, 2026   Volume 21, Issue 7 e0352182 doi: 10.1371/journal.pone.0352182
Cranston N, Buckley P, Rose L, Loy J, Randle H.Weaning is a stressful time in a foal's (Equus caballus) life. As humans artificially manage weaning in domestic horses, it provides an opportunity to safeguard horse welfare early in life, with potential long-term benefits. While numerous studies have examined the effects of different weaning and management interventions on foal stress, optimal weaning practices have yet to be identified. Overall purpose of the study: To identify which weaning interventions and management practices have the greatest potential to improve foal welfare based on physiological and behavioural outcomes. Research qu...
Development of an indirect ELISA for the detection of venezuelan equine encephalitis virus specific antibodies in horses.
PloS one    June 10, 2026   Volume 21, Issue 6 e0338819 doi: 10.1371/journal.pone.0338819
Chevrier M, Mathews-Martin L, Mariteragi-Helle T, Dumarest M, Boudjadi Y, Bréard E, Turpaud M, Deshayes T, Vissani MA, Vinueza RL, Coello Peralta R....Venezuelan equine encephalitis virus (VEEV) is a re-emerging zoonotic pathogen to which equines are most susceptible mammals hosts with a high mortality rate raging from19 to 83%. The virus, transmitted between vertebrates and mosquitos, causes unpredictable sporadic epizootic outbreaks in equids and humans across Central and South America. Although VEEV is not currently circulating in Europe the combination of global change, increasing international trade and the presence of potentially competent vectors (mosquitoes of the genera Aedes and Culex), raises a non-negligible risk of its introduct...
Ovarian follicle dynamics in the plains zebra (Equus quagga).
PloS one    May 22, 2026   Volume 21, Issue 5 e0348772 doi: 10.1371/journal.pone.0348772
Barros LO, Hyde KA, Gazzaniga N, Gastal MO, Aguiar FLN, Carneiro GF, Gastal EL.Understanding follicular dynamics during the estrous cycle is essential for developing breeding programs and assisted reproductive technologies (ARTs) in any species. In this context, knowledge of follicular dynamics and related uterine changes in the plains zebra (Equus quagga) is essential to increase the declining population of this vital African species and to provide comparative insights into more endangered zebra species. Likely due to the difficulty of handling these wild species, no detailed reproductive study using ultrasonography has been performed in any zebra. Therefore, the presen...
A service provider perspective in Irish horseracing on the availability of retirement specific support services for professional jockeys and perceived barriers and facilitators to their use.
PloS one    April 30, 2026   Volume 21, Issue 4 e0348055 doi: 10.1371/journal.pone.0348055
Langton L, Cullen SJ, Warrington G, Pugh J, McGoldrick A, Losty C.Retirement can come at any time in horse racing due to the unpredictable nature of the sport. Preplanning for the retirement transition in sport has been reported to lead to faster adjustment to retirement and better transition for athletes. The purpose of this study was to highlight the current support services available to Irish horseracing jockeys to aid with career transitions and to a) explore perceived barriers and b) perceived facilitators to their use. Semi-structured interviews were conducted with 11 representatives from various racing bodies including the Irish Horse Racing Regulator...
Social enrichment mitigates facial expressions and physiological indicators of short-term stress in horses.
PloS one    April 20, 2026   Volume 21, Issue 4 e0347571 doi: 10.1371/journal.pone.0347571
Bini de Lima AC, Sebastião da Fé VC, Palermo Hernandes MS, Pfeifer de Cristo EC, Dos Santos Fagundes Euzébio AG, E Silva Sousa MV....This study aimed to evaluate the ability of social noncontact environmental enrichment to facilitate social buffering and to characterize the emotional experience of horses subjected to restraint in stock by assessing physiological parameters and facial expressions. Pantaneiro horses (n = 11) were evaluated in a crossover design with two treatments: social noncontact enrichment during stock restraint and social isolation during stock restraint. Physiological parameters (heart rate, heart rate variability, respiratory rate, ocular temperature by infrared thermography, and auricular temperat...
Influence of riders’ physical activity on improving seat balance in the context of horse welfare – A pilot study.
PloS one    April 15, 2026   Volume 21, Issue 4 e0344969 doi: 10.1371/journal.pone.0344969
Zeliszewska-Duk P, Skowerska-Wiśniewska A, Wilk I, Nowicka B, Duk M, Strzelec K.This study aimed to investigate the impact of gym exercises for riders on their posture and the subsequent effect on reducing discomfort for their horses during training, using electronic pressure sensors. The study involved 20 warmblood horses aged 5-10 years, regularly ridden under saddle, and four riders of similar height and body weight. The study group was divided into a control group (10 horses) and an experimental group (10 horses). The horses were trained 5 days a week for 60 days in total. Riders trained three times a week for approximately 60 minutes. The exercises were designed to i...
The transition from winter to spring has an impact on the airway metabolome profile of asthmatic horses.
PloS one    April 3, 2026   Volume 21, Issue 4 e0346250 doi: 10.1371/journal.pone.0346250
Drespling J, Heelemann S, Strathmeyer S, Kühn H, Schwarz B, Mundhenk L.Equine asthma is a chronic, non-infectious inflammatory disease of the lower airways in horses, classified as mild to moderate (MEA) or severe (SEA). Its pathogenesis is not fully understood and is influenced by environmental and seasonal factors. In this cross-sectional study, seasonal effects on the bronchoalveolar lavage fluid (BALF) metabolome were investigated in asthmatic and non-asthmatic horses. The metabolome of 230 BALF samples from horses across different seasons, classified as cytologically unremarkable (CUA), MEA, or SEA, was analyzed using proton nuclear magnetic resonance spectr...
Saliva and plasma metabolome analysis during the five days before foaling in the mare.
PloS one    April 1, 2026   Volume 21, Issue 4 e0344530 doi: 10.1371/journal.pone.0344530
Nadal-Desbarats L, Dupuy C, Montigny F, Bertevello PS, Douet C, Gesbert A, Reigner F, Goudet G.Saliva is a relevant biofluid for real-time welfare-friendly monitoring of systemic events in animals, because some bioanalytes have a systemic origin and its collection is painless, stress-free and non-invasive. Our aim was to analyze the metabolome of equine saliva during the five days before foaling, with a focus on identifying metabolites whose level significantly changed before parturition, that could be potential salivary biomarkers of the onset of parturition. We compared the saliva and plasma metabolomes to investigate their relationship. Saliva and blood samples were collected from tw...
Computational modelling of the equine arteritis virus GP5/M Dimer: Implications for immune evasion and virulence.
PloS one    March 10, 2026   Volume 21, Issue 3 e0344287 doi: 10.1371/journal.pone.0344287
Veit M, Matczuk AK.Equine arteritis virus (EAV) is a positive-stranded RNA virus of the Arteriviridae family. Its GP5/M dimer, the principal component of the viral envelope, mediates virus budding and serves as a key target for neutralizing antibodies. Using AlphaFold3, we predicted the 3D structure of the EAV GP5/M dimer and compared it to its homolog in porcine reproductive and respiratory syndrome virus (PRRSV). Both complexes share a conserved architecture comprising a short ectodomain, three helical transmembrane regions, and a β-sheet-rich endodomain. EAV GP5 features a longer ectodomain with four α-heli...
Rider education at Swedish riding schools: Comparing teachers’ and pupils’ perspectives.
PloS one    February 27, 2026   Volume 21, Issue 2 e0331059 doi: 10.1371/journal.pone.0331059
Nyberg L, Blokhuis MZ, McLean A, Hartmann E.Previous research has highlighted a gap between scientific evidence and its application in equestrian practice, leading to concerns about horse welfare and human safety. Riding schools present an important platform for promoting science-based practices, as they bring together riders of all ages and levels and serve as sites for equestrian education and for shaping attitudes toward horse welfare. Yet, the teaching in riding schools is often rooted in traditional practices. Therefore, mapping current educational methods and exploring how teaching and learning are perceived by both riding school ...
Obtention and preliminary clinical evaluation of an equine albumin for intravenous administration in adult Colombian Creole Horses (Equus ferus caballus).
PloS one    February 20, 2026   Volume 21, Issue 2 e0341577 doi: 10.1371/journal.pone.0341577
Cifuentes V, Zuluaga-Cabrera AM, Vargas-Muñoz LJ, Estrada-Gómez S.Albumin is one of the most abundant and physiologically important blood protein in horses due to its ability to regulate vascular volume and transport blood metabolites or drugs. Despite the importance of this protein, in Colombia there is no previous reference of the use of equine albumin in horses as a pharmacological therapy and there is no available any pharmaceutical preparation of this protein to be administrated in horses. This study aims to evaluate for first time the preliminary clinical response of healthy adult Colombian Creole horses after the intravenous administration of an equin...
Beyond the host: Unveiling the independent microbiome of equine gastrointestinal nematodes.
PloS one    February 10, 2026   Volume 21, Issue 2 e0339596 doi: 10.1371/journal.pone.0339596
Gentilini F, Ogundipe TG, Turba ME, Romagnoli N, Lambertini C, Pollera C, Cremonesi P, Stancampiano L.Gastrointestinal nematode infections significantly impact equine health and welfare, with rising anthelmintic resistance demanding alternative control strategies. Emerging evidence suggests that parasitic nematodes harbour distinct microbiomes, potentially influencing host-parasite dynamics and parasite survival. This study aimed to characterize and compare the microbiomes of equine gastrointestinal nematodes and their hosts, focusing on differences in composition, diversity, and core microbiota structure across different intestinal sites, nematode subfamilies, and sexes. Faecal and nematode s...
Aberrant skeletal muscle morphogenesis and myofiber differentiation characterize equine myotonic dystrophy.
PloS one    January 29, 2026   Volume 21, Issue 1 e0341655 doi: 10.1371/journal.pone.0341655
Valberg SJ, Williams ZJ, Ames EG, Mickelson JR, Nout-Lomas YS, Landolt G, Sanz M, Gardner K.Equine myotonic dystrophy (eMD) is a rare neuromuscular disorder of undetermined origin marked by muscle hypertrophy and stiffness, dystrophic muscle histopathology, and myotonic discharges. In humans, myotonic dystrophy (DM) arises from trinucleotide repeat expansions in dystrophia myotonica protein kinase (DMPK) (DM1) or tetranucleotide expansions in cellular nucleic acid-binding protein (CNBP) (DM2), which disrupt mRNA processing and induce embryonic splicing patterns across multiple genes. In 6 eMD Quarter Horse types, (2-36 months-of-age) and 8 control Quarter Horses we determined: (1) fi...
Human emotional odours influence horses’ behaviour and physiology.
PloS one    January 14, 2026   Volume 21, Issue 1 e0337948 doi: 10.1371/journal.pone.0337948
Jardat P, Destrez A, Damon F, Tanguy-Guillo N, Lainé AL, Parias C, Reigner F, Ferreira VHB, Calandreau L, Lansade L.Olfaction is the most widespread sensory modality animals use to communicate, yet much remains to be discovered about its role. While most studies focused on intraspecific interactions and reproduction, new evidence suggests chemosignals may influence interspecific interactions and emotional communication. This study explores this possibility, investigating the potential role of olfactory signals in human-horse interactions. Cotton pads carrying human odours from fear and joy contexts, or unused pads (control odour) were applied to 43 horses' nostrils during fear tests (suddenness and novelty ...
Detection of vasculogenic mimicry in equine ocular, oronasal, and genital squamous cell carcinoma.
PloS one    January 5, 2026   Volume 21, Issue 1 e0328584 doi: 10.1371/journal.pone.0328584
Schwarz S, Kummer S, Klang A, Walter I, Nell B, Brandt S.Squamous cell carcinoma (SCC) is the most common malignant tumor disease in horses. It predominantly affects the ocular, oronasal, and anogenital region. Equine SCC is difficult to treat, also because important aspects of SCC development and metastasis are still unclear. We previously provided evidence that equine SCC cells can adopt a stem cell-like phenotype as a hallmark of malignant progression. Here, we investigated whether equine SCCs harbor endothelial-like tumor cells that form an alternative network of pseudo-vessels better known as vasculogenic mimicry (VM). Following histopathologic...
Genomic epidemiology of strains currently and formerly classified as Enterobacter spp. recovered from equine necropsy samples.
PloS one    November 13, 2025   Volume 20, Issue 11 e0333701 doi: 10.1371/journal.pone.0333701
Harel B, Sévin C, Le Hello S, Moreau P, Giard JC, Petry S, Gravey F.Enterobacteriaceae are opportunistic pathogens responsible for local or systemic infections in both human and veterinary medicine. To monitor circulating strains in stud farms in Normandy (France), we investigated a collection of Enterobacteriaceae isolated from necropsied equids performed in the region between 1997 and 2020. These strains were initially identified using MALDI-TOF; however, as this method failed to identify some isolates, whole genome sequencing followed by rMLST analysis was subsequently performed. Different genera were identified: Enterobacter spp., Huaxiibacter spp., Lellio...
Collagen composition in equine exuberant granulation tissue reflects tissue immaturity.
PloS one    November 6, 2025   Volume 20, Issue 11 e0335179 doi: 10.1371/journal.pone.0335179
Partusch L, Rutland CS, Martens A, Du Cheyne C, De Spiegelaere W, Michler JK.Exuberant granulation tissue (EGT) is a second intention wound healing disorder. It commonly occurs in the distal limb of horses. EGT causes significant increase in the duration and cost of treatment, potentially leading to the decision not to pursue treatment and euthanize the patient. The underlying pathomechanisms of this fibroproliferative disorder remain unclear, particularly in terms of collagen composition and the association between myofibroblasts and blood vessels. This study investigated the collagen composition in naturally occurring EGT following trimming in 19 horses (EGT group). ...
Exploring the association between stress-related hormonal changes, behaviours and facial movements after an interval training exercise in French Standardbred.
PloS one    November 5, 2025   Volume 20, Issue 11 e0328430 doi: 10.1371/journal.pone.0328430
Hennes N, Tutin L, Foury A, Vancassel S, Bourguignon H, Duluard A, Ruet A, Lansade L.Physical exercise can act as a physiological and a mental stressor. Monitoring exercise-induced stress is therefore essential to understand racehorses 'responses to effort and to ensure their welfare. Stress perceived by the horse during physical activity can be measured using various indicators, including stress-related hormones such as cortisol and adrenaline, and other neuromodulators such as serotonin, all involved in the stress response and its regulation. Another approach to assess physiological and emotional responses to stimuli such as exercise is through behaviours and facial movement...
A move in the right direction: Tracking the traceability of British Thoroughbreds outside of racing.
PloS one    September 19, 2025   Volume 20, Issue 9 e0331968 doi: 10.1371/journal.pone.0331968
Williams JM, Jordan S, Friend L, Kay E, Edmunds M, Flynn H, Wensley S.Horse welfare within/after racing is often questioned by the public. British Racing's Horse Welfare Board's "A life well-lived" strategy provides a blueprint for Thoroughbred welfare, advocating accurate lifetime traceability of horses as essential to achieve this. The Census aimed to establish a population density model for British Thoroughbreds, not actively engaged in racing. Equestrians who owned/kept a Thoroughbred were asked to complete the Census between May and December 2023. Frequency analysis identified patterns in passport compliance, knowledge and understanding of current systems, ...
Normal MRI features of the manica flexoria in horses and evaluation of the anatomic variability between forelimbs and hindlimbs.
PloS one    July 21, 2025   Volume 20, Issue 7 e0327880 doi: 10.1371/journal.pone.0327880
Miles S, McCauley C, Carossino M, Del Piero F, Liu CC, Gaschen L.Manica flexoria tears are increasingly recognized as a cause of lameness in horses resulting in a need for improved pre-operative diagnosis. Partial tears are difficult to pre-operatively diagnose and thus research for diagnostics of manica flexoria tears has been seen in increasing frequency over the past decade, though a deficit of information exists for the features of this structure on MRI, which is best suited for evaluation of soft tissues. The goal is to perform an observational study on the morphometry of the normal manica flexoria prospectively and describe it retrospectively on MRI. ...
Metabolomics analysis and mRNA/miRNA profiling reveal potential cardiac regulatory mechanisms in Yili racehorses under different training regimens.
PloS one    July 14, 2025   Volume 20, Issue 7 e0322468 doi: 10.1371/journal.pone.0322468
Wang T, Meng J, Peng X, Huang J, Huang Y, Yuan X, Li X, Yang X, Chang X, Zeng Y, Yao X.Yili horses, a versatile breed from Xinjiang, China, are renowned for their racing abilities. However, studies on the links between cardiac morphology, function, and metabolic profiles with performance are limited. This study combined echocardiographic, transcriptomic, and metabolomic analyses to explore these relationships in high-level, average, and untrained Yili horses. Echocardiographic assessments revealed increased left ventricular mass in trained horses, with significant differences in intraventricular septal thickness and left ventricular end-diastolic diameter. RNA sequencing identif...
Influence of lighting on sleep behaviour, circadian rhythm and spontaneous blink rate in stabled riding school horses (Equus caballus).
PloS one    June 27, 2025   Volume 20, Issue 6 e0326567 doi: 10.1371/journal.pone.0326567
Greening L, Harkin E, Kyriazopoulou P, Heppelthwaite Z, Aragona F, Browne JA, Hemmings A, Williams JM, Murphy BA.Modern horse husbandry involves significant time spent indoors, often in suboptimal lighting conditions and with frequent night-time disturbances by humans for management purposes. The aim of this study was to investigate the influence of a customised light-emitting diode (LED) lighting system and a standard fluorescent lighting fixture on equine sleep behaviours, circadian rhythmicity and spontaneous blink rates in horses. Ten riding school horses experienced two stable lighting conditions for four weeks each in a cross-over study running from January to March, 2023. The treatment lighting co...
Impact of feeding strategies on the welfare and behaviour of horses in groups: An experimental study.
PloS one    June 25, 2025   Volume 20, Issue 6 e0325928 doi: 10.1371/journal.pone.0325928
Roig-Pons M, Bachmann I, Freymond SB.Finding feeding strategies that meet horses' needs without compromising health is essential for optimising welfare, particularly in group housing, where limited hay availability increases aggression and injury risks. Recently, two strategies have emerged: portioning daily intake into smaller, frequent meals using time-controlled hay racks, or slowing intake with "slow-feeders." However, the effects of such management practices on horse behaviour remain underexplored. We conducted a cross-over study with 18 mares divided into four groups to compare three feeding strategies: "traditional" (3 of ...
Correction: Expression of genes with biomarker potential identified in skin from DSLD-affected horses increases with age.
PloS one    June 16, 2025   Volume 20, Issue 6 e0326448 doi: 10.1371/journal.pone.0326448
Roberts JH, Zhang J, David F, McLean A, Blumenshine K, Müller-Alander E, Halper J.[This corrects the article DOI: 10.1371/journal.pone.0287740.].
Effects of jockey position and surfaces on horse movement asymmetry and horse-jockey synchronisation during trotting exercise.
PloS one    June 2, 2025   Volume 20, Issue 5 e0324753 doi: 10.1371/journal.pone.0324753
Horan K, Pfau T.Racehorses and jockeys can incur injuries, not only during gallops, but also during routine trotting exercise to access gallop tracks or warm-up. Understanding how jockey position affects racehorse movement may influence safety, and this may vary across different surface conditions. This study used inertial sensing technology (XSens MTw sensors) and linear mixed models to quantify and determine the significance (p ≤ 0.05) of jockey riding position ('rising' versus 'two-point seat') and surface type (artificial, grass and tarmac) on: 1) time offsets between stance and flight phases; 2) ho...
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