Analyze Diet

PloS one.

Periodical
Medicine
Science
Publisher:
Public Library of Science
Frequency: Monthly
Country: United States
Language: English
Author(s):
Public Library of Science.
Start Year:2006 -
Identifiers
ISSN:1932-6203 (Electronic)
1932-6203 (Linking)
NLM ID:101285081
(OCoLC):70662135
LCCN:2006214532
Risk factors for, metrics of, and consequences of access to veterinary care for companion animals: A scoping review.
PloS one    May 30, 2025   Volume 20, Issue 5 e0325455 doi: 10.1371/journal.pone.0325455
O'Connor A, Totton SC, Hernandez M, Meyers E, Meyers K, Abreu HM, Spofford N, Morrison J.Barriers to accessing veterinary care can be challenging for companion-animal caregivers and may lead to preventable health conditions or even death of pets. Objective: We conducted a scoping review to: 1) catalog the definitions of access to veterinary care (A2VC) used by researchers, 2) identify risk factors for and consequences of A2VC, and 3) map the risk factors onto dimensions of access to care (affordability, availability, accessibility, accommodation, acceptability). Methods: Primary research on companion animals not involved in commercial enterprises (e.g., horse racing) examining con...
Effect of orally administered cannabidiol oil on daily tonometric curve in healthy Italian Saddle horses.
PloS one    May 28, 2025   Volume 20, Issue 5 e0325191 doi: 10.1371/journal.pone.0325191
Bazzano M, Laus F, Cerquetella M, Spaterna A, Marchegiani A.Phytocannabinoids have the potential to lower intraocular pressure in both normal and glaucomatous eyes and they have been tested in different animal species, but not in the horse. The present paper describes the tonometric curve of healthy adult Italian Saddle horses after oral administration of cannabidiol oil (CBD). Methods: CBD 20% was administered orally (oily solution) at the dose of 1 mg/kg to 8 adult horses and intraocular pressure (IOP) was evaluated by tonometric curve. Data were then compared to those of the same horses obtained the day before (blank) CBD administration. Results: ...
Auditory enrichment on facial and physiological responses of Pantaneiro geldings and mares under short-term stress.
PloS one    May 20, 2025   Volume 20, Issue 5 e0323649 doi: 10.1371/journal.pone.0323649
da Fé VCS, Dos Santos VMO, de Lima ACB, Hernandes MSP, Caldara FR, Gomes MNB.This study aimed to evaluate the effect of auditory stimuli on mares and geldings during social isolation and movement restriction, and their ability to promote the reduction of stress responses. The research was conducted with eight Pantaneiro horses, divided into Experiment I (4 mares) and Experiment II (4 geldings), both experiments were executed equally using a 4x4 Latin square design with experimental (classical, country and new age) and control (no music) treatments. Physiological parameters (heart rate, heart rate variability, respiratory rate, ocular temperature by infrared thermograph...
Characterisation of facial expressions and behaviours of horses in response to positive and negative emotional anticipation using network analysis.
PloS one    May 14, 2025   Volume 20, Issue 5 e0319315 doi: 10.1371/journal.pone.0319315
Phelipon R, Bertrand L, Jardat P, Reigner F, Lewis K, Micheletta J, Lansade L.The welfare of an animal is closely linked to their emotional experiences, making it essential to identify reliable indicators of these emotions. This study aimed to identify behaviours and facial movements in horses experiencing contrasting emotional valence, triggered by the anticipation of a positive condition (going to pasture) or a negative condition (going alone to a novel environment). Twenty horses were daily trained to wait in a starting box before being exposed to these two conditions. After one week of positive training or negative training, we analysed horses' behaviours, cortisol ...
Contributing to evidence-based veterinary medicine: A qualitative study of veterinary professionals’ views and experiences of client-owned companion animal research.
PloS one    May 9, 2025   Volume 20, Issue 5 e0322902 doi: 10.1371/journal.pone.0322902
Furtado T, Perkins E, Archer DC.Research on the outcomes of veterinary treatments in dogs, cats and horses has important benefits for these animals and their owners. However, this information is not always available, and the evidence base is often lower-quality than in human medicine. To identify ways to improve the generation of evidence, we investigated the views of veterinary professionals about research involving companion animal patients and their owners. This qualitative study used semi-structured interviews with veterinary surgeons and registered veterinary nurses working in UK companion animal clinical practice. Inte...
Retrospective radiographic myelogram measurements and long-term outcomes in horses undergoing cervical interbody fusion surgery: 22 cases.
PloS one    May 7, 2025   Volume 20, Issue 5 e0323083 doi: 10.1371/journal.pone.0323083
England D, Newsom L, White C, McKenzie E.Site selection for cervical stabilization surgery in horses with spinal ataxia frequently relies on measurements derived from radiographic myelography. A variety of measurement criteria exist and can provide conflicting results. The main objectives of this study were to assess the correlation between two commonly used myelographic measures, dorsal contrast column reduction (DCCR) and dural diameter reduction (DDR), and their association with previously selected operative sites in a population of horses operated at a tertiary clinic. Secondary objectives were to determine if articular process j...
3000 yr-old patterns of mobile pastoralism revealed by multiple isotopes and radiocarbon dating of ancient horses from the Mongolian Altai.
PloS one    May 7, 2025   Volume 20, Issue 5 e0322431 doi: 10.1371/journal.pone.0322431
Zazzo A, Le Corre M, Lazzerini N, Marchina C, Bayarkhuu N, Bernard V, Cervel M, Fiorillo D, Joly D, Lemoine M, Telouk P, Thil F, Turbat T, Balter V....Pastoral nomadism is of great cultural and economic importance in several regions of the world today. However, documenting ancient patterns of mobility in societies where pastoralism was central is challenging and requires tailored approaches and methodologies. Here we use strontium, oxygen and carbon isotopic analyses of dental enamel, together with a local strontium isoscape, to reconstruct the mobility patterns of seven domestic horses deposited in a Late Bronze Age grave from western Mongolia. Radiocarbon indicates that the animals were deposited within a short period of time, 3000 years a...
Metagenomic and proteomic analyses reveal similar reproductive microbial profiles and shared functional pathways in uterine immune regulation in mares and jennies.
PloS one    April 16, 2025   Volume 20, Issue 4 e0321389 doi: 10.1371/journal.pone.0321389
da Silva-Álvarez E, Gómez-Arrones V, Correa-Fiz F, Martín-Cano FE, Gaitskell-Phillips G, Carrasco JJ, Rey J, Aparicio IM, Peña FJ, Alonso JM....This study aims to unveil potential differences in the vaginal and uterine microbiomes in mares and jennies, and to identify possible mechanisms involved in uterine immune homeostasis. The microbiota was characterized using 16S rRNA sequencing, and the uterine proteome was analyzed using UHPLC/MS/MS in 18 samples from healthy mares and 14 from jennies. While taxonomic analysis revealed high interspecies similarities, β-diversity analysis showed distinct clustering, with only two vaginal taxa and five uterine taxa differing between species. Despite compositional differences, PICRUSt analysis s...
Detection of mare parturition through balanced multi-scale feature fusion based on improved Libra RCNN.
PloS one    March 4, 2025   Volume 20, Issue 3 e0318498 doi: 10.1371/journal.pone.0318498
Wang B, Duan W, Zhao J, Bai D.Once a mare experiences parturition abnormalities, the outcome between a live foal and a stillborn can change rapidly. Automated detection of mare parturition and timely human intervention is crucial to reducing risks during mare and foal parturition. This paper addresses the challenges of manual monitoring of parturition in large-scale equine facilities due to the unpredictability of mare parturition timing, proposing an algorithm for detecting mare parturition through a balanced multi-scale feature fusion based on an improved Libra RCNN. Initially, a ResNet101 backbone network incorporating ...
Correction: Automated recognition of emotional states of horses from facial expressions.
PloS one    February 12, 2025   Volume 20, Issue 2 e0319501 doi: 10.1371/journal.pone.0319501
Feighelstein M, Ricci-Bonot C, Hasan H, Weinberg H, Rettig T, Segal M, Distelfeld T, Shimshoni I, Mills DS, Zamansky A.[This corrects the article DOI: 10.1371/journal.pone.0302893.].
Effect of the intrinsic and extrinsic factors on the growth and development of young foals under subtropical conditions of Pakistan.
PloS one    January 30, 2025   Volume 20, Issue 1 e0310784 doi: 10.1371/journal.pone.0310784
Chatha MA, Ahmad N, Abbas MA, Saadullah M, Khan JA.This study was designed to explore the impact of intrinsic (breed of foal, age of dam, and age of foal at weaning) and extrinsic (season of birth and housing type) factors on the growth and survival of foals in the subtropical conditions of Pakistan. For the growth study, retrospective data analysis of foals (n = 150) born from purebred brood mares of Thoroughbred, Arabs, and Percheron breeds (n1, n2, and n3 = 50 each) was made. Six hundred and twenty-four (n = 624) foals born between 2020 to 2022 were observed for the study of foal survival rate. The survival of these foals till the age of on...
Nebulization of 2% lidocaine has no detectable impact on the healthy equine respiratory microbiota.
PloS one    January 24, 2025   Volume 20, Issue 1 e0316079 doi: 10.1371/journal.pone.0316079
Holley L, Creasey HN, Bedenice D, Reed S, Romualdo da Silva DR, Trautwein V, Mazan M, Widmer G.Glucocorticosteroids remain the most common pharmaceutical approach for the treatment of equine asthma but can be associated with significant side effects, including respiratory microbiome alterations. The goal of the study was to assess the impact of 2% lidocaine nebulization, a projected alternative treatment of equine asthma, on the healthy equine respiratory microbiota. A prospective, randomized, controlled, blinded, 2-way crossover study was performed, to assess the effect of 1 mg/kg 2% lidocaine (7 treatments over 4 days) on the equine respiratory microbiota compared to control horses (s...
Exploring a pico-well based scRNA-seq method (HIVE) for simplified processing of equine bronchoalveolar lavage cells.
PloS one    January 24, 2025   Volume 20, Issue 1 e0317343 doi: 10.1371/journal.pone.0317343
Fegraeus K, Riihimäki M, Nordlund J, Akula S, Wernersson S, Raine A.Single-cell RNA sequencing (scRNA-seq) is a valuable tool for investigating cellular heterogeneity in diseases such as equine asthma (EA). This study evaluates the HIVE™ scRNA-seq method, a pico-well-based technology, for processing bronchoalveolar lavage (BAL) cells from horses with EA. The HIVE method offers practical advantages, including compatibility with both field and clinical settings, as well as a gentle workflow suited for handling sensitive cells. Our results show that the major cell types in equine BAL were successfully identified; however, the proportions of T cells and macropha...
Respiratory extracellular vesicle isolation optimization through proteomic profiling of equine samples and identification of candidates for cell-of-origin studies.
PloS one    January 24, 2025   Volume 20, Issue 1 e0315743 doi: 10.1371/journal.pone.0315743
Hickman E, Carberry V, Carberry C, Cooper B, Mordant AL, Mills A, Sokolsky M, Herring LE, Alexis NE, Rebuli ME, Jaspers I, Sheats K, Rager JE.Growing evidence supports the importance of extracellular vesicle (EV) as mediators of communication in pathological processes, including those underlying respiratory disease. However, establishing methods for isolating and characterizing EVs remains challenging, particularly for respiratory samples. This study set out to address this challenge by comparing different EV isolation methods and evaluating their impacts on EV yield, markers of purity, and proteomic signatures, utilizing equine/horse bronchoalveolar lavage samples. Horses can serve as effective translational animal models for respi...
Factors associated with time to return to horse racing following a clavicle fracture in jockeys competing in Great Britain: A review and analysis of medical records.
PloS one    January 23, 2025   Volume 20, Issue 1 e0317724 doi: 10.1371/journal.pone.0317724
Newton LJ, Dobbin N, Goodwin P, Crampton JS.Competitive horse racing is the second largest sport in Great Britain by spectator attendance, employability, and revenue. It is a lucrative yet hazardous sport, with high injury rates, particularly from falls. Clavicular fractures are one of the most common injuries reported, yet their management, especially regarding return to racing, is under-researched. The purpose of this study was to explore the factors associated with the time to return to competition following a clavicular fracture in jockeys competing in Great Britain. Methods: This review of medical records utilised data from the Bri...
Whole genome sequences of nine Taylorella equigenitalis strains isolated in the Czech Republic between 1982-2021: Molecular dating suggests a common ancestor at the time of Roman Empire.
PloS one    January 3, 2025   Volume 20, Issue 1 e0315946 doi: 10.1371/journal.pone.0315946
Hrala M, Andrla P, Bosák J, Fedrová P, Mugutdinov A, Karpíšková R, Nedbalcová K, Raichová J, Faldyna M, Hořín P, Šmajs D.Taylorella equigenitalis is the causative agent of sexually transmitted contagious equine metritis. Infections manifest as cervicitis, vaginitis and endometritis and cause temporary infertility and miscarriages of mares. While previous studies have analyzed this organism for various parameters, the evolutionary dynamics of this pathogen, including the emergence of antibiotic resistance, remains unresolved. The aim of this study was to isolate contemporary strains, determine their genome sequences, evaluate their antibiotic resistance and compare them with other strains. We determined nine comp...
Protocol to implement and evaluate a culturally secure, strength-based, equine-assisted learning program, “Yawardani Jan-ga” (horses helping), to support the social and emotional wellbeing of Australian aboriginal children and young people.
PloS one    December 30, 2024   Volume 19, Issue 12 e0312389 doi: 10.1371/journal.pone.0312389
Coffin J, Vaz S, Kickett-Tucker C, Milroy H, Olsson C, Kirby M, Nelson L, McPhee R, Cross D.Australian Aboriginal people experience stressors from inequalities across crucial social determinants, including deep and entrenched disadvantage and exclusion. The impact of unaddressed historical issues is pervasive and intergenerational. The disproportionate rates of Aboriginal youth suicide, juvenile detention and imprisonment highlight the inadequacy of existing social and emotional wellbeing programs and services for Aboriginal children and young people. There is increasing recognition in Australia that aligning social and emotional wellbeing interventions with Western values and concep...
A comparative study evaluating three line immunoassays available for serodiagnosis of equine Lyme borreliosis: Detection of Borrelia burgdorferi sensu lato-specific antibodies in serum samples of vaccinated and non-vaccinated horses.
PloS one    December 23, 2024   Volume 19, Issue 12 e0316170 doi: 10.1371/journal.pone.0316170
Broeckl CV, Hiereth S, Straubinger RK.Diagnosis of equine Lyme borreliosis (LB), an infection caused by members of the Borrelia burgdorferi sensu lato complex (Bbsl), is challenging due to the nonspecific clinical signs of the disease and due to the variety of non-standardized serological tests. Specific vaccine-induced antibodies against LB, providing an effective protection against the infection, complicate the issue further. The standard for the detection of specific antibodies against Bbsl is a two-tier test system based on an enzyme-linked immunosorbent assay (ELISA) or indirect fluorescent antibody test (IFA) for antibody sc...
Anesthesia for non-obstetric surgery during late term pregnancy in mares.
PloS one    November 22, 2024   Volume 19, Issue 11 e0313563 doi: 10.1371/journal.pone.0313563
Brito PHS, Ferreira MA, Rusch E, Arantes JA, Carregaro AB, Valadão CAA, Ghantous GF, Dória RGS.Submitting late-term pregnant mares to anesthesia for non-obstetric surgery raises concerns about the survival of the mother and fetus. This study aimed to evaluate and describe transient maternal and fetal hemodynamic changes during general inhalation anesthesia in mares during the last month of gestation. Nine adult mares in the last month of gestation were subjected to general inhalation anesthesia and dorsal recumbency for 90 minutes. Trans-anesthetic vital parameters, arterial hemogasometry, cardiac output, pulmonary arterial pressure, central venous pressure, and fetal heart rates were a...
Treatment approaches to horses with acute diarrhea admitted to referral institutions: A multicenter retrospective study.
PloS one    November 20, 2024   Volume 19, Issue 11 e0313783 doi: 10.1371/journal.pone.0313783
Gomez DE, Kopper JJ, Byrne DP, Renaud DL, Schoster A, Dunkel B, Arroyo LG, Mykkanen A, Gilsenan WF, Pihl TH, Lopez-Navarro G, Tennent-Brown BS....This study aimed to describe and compare therapeutic approaches for horses with acute diarrhea in different geographic regions worldwide. Methods: Clinical information was retrospectively collected from diarrheic horses presented to participating institutions between 2016 and 2020, including fluid therapy on admission, antimicrobial drugs, probiotics, anti-endotoxic medications, anti-inflammatory drugs, gastroprotectants, digital cryotherapy, and toxin-binding agents. Seasonal and geographic differences were investigated. Results: 1438 horses from 26 participating hospitals from 5 continents w...
Equine bone marrow-derived mesenchymal stromal cells reduce established S. aureus and E. coli biofilm matrix in vitro.
PloS one    October 31, 2024   Volume 19, Issue 10 e0312917 doi: 10.1371/journal.pone.0312917
Khatibzadeh SM, Dahlgren LA, Caswell CC, Ducker WA, Werre SR, Bogers SH.Biofilms reduce antibiotic efficacy and lead to complications and mortality in human and equine patients with orthopedic infections. Equine bone marrow-derived mesenchymal stromal cells (MSC) kill planktonic bacteria and prevent biofilm formation, but their ability to disrupt established orthopedic biofilms is unknown. Our objective was to evaluate the ability of MSC to reduce established S. aureus or E. coli biofilms in vitro. We hypothesized that MSC would reduce biofilm matrix and colony-forming units (CFU) compared to no treatment and that MSC combined with the antibiotic, amikacin sulfate...
Working horse welfare in Senegal is linked to owner’s socioeconomic status, their attitudes and belief in horse sentience.
PloS one    October 18, 2024   Volume 19, Issue 10 e0309149 doi: 10.1371/journal.pone.0309149
Seck M, Carder G, Wathan J, Randau M, Fletcher K, Proops L.The role that working equids play in both rural and urban communities in low and middle-income countries is invaluable. They contribute to daily tasks such as carrying food, water and people, support income generation, and are of social and cultural importance. Despite their importance, global standards of working equid welfare are low. Many variables can impact the welfare status of animals under human care, but often specific factors are explored in isolation. Factors can include, but are not limited to an owner's socioeconomic status and their attitudes and beliefs towards animals. In this ...
Rein tensions and behaviour with five rein types in international-level vaulting horses.
PloS one    October 17, 2024   Volume 19, Issue 10 e0311919 doi: 10.1371/journal.pone.0311919
Biau S, Pycik E, Boichot L, Berg LC, Ruet A.Health and performance of vaulting horses cantering with reins might be affected by rein tensions. The primary aim of this present study was to measure rein and lunge line tensions in international-level vaulting horses with several types of reins adjusted in accordance with the requirements of the FEI Vaulting Rules and study the effect of reins types on it. The secondary aim was to evaluate behavioural signs of discomfort under the same conditions and study the effect of reins types on it. The relationship between tensions and behavioural signs of discomfort was then explored. Thirty-nine in...
Distal sagittal forelimb conformation in young Walloon horses: Radiographic assessment and its relationship with osteochondral fragments.
PloS one    October 11, 2024   Volume 19, Issue 10 e0311965 doi: 10.1371/journal.pone.0311965
Van Cauter R, Caudron I, Lejeune JP, Rousset A, Serteyn D.Osteochondral fragments within equine joints are commonly encountered and may predispose to lameness and limitation to sport purposes. Factors leading to this condition include genetic, nutritional and environmental conditions. However, few studies have evaluated the impact of conformation traits and their correlation with osteochondrosis. This study, based on the radiographic screenings of young horses born in Wallonia (266 individuals, 532 forelimbs), evaluated the correlation between foot, fetlock conformations of the front limb, height at the withers and presence of osteochondral fragments...
Hoof slip duration at impact in galloping Thoroughbred ex-racehorses trialling eight shoe-surface combinations.
PloS one    October 11, 2024   Volume 19, Issue 10 e0311899 doi: 10.1371/journal.pone.0311899
Horan K, Coburn J, Kourdache K, Day P, Carnall H, Brinkley L, Harborne D, Hammond L, Millard S, Weller R, Pfau T.Horseshoes used during racing are a major determinant of safety as they play a critical role in providing traction with the ground surface. Although excessive hoof slip is detrimental and can predispose to instabilities, falls and injuries, some slip is essential to dissipate energy and lower stresses on the limb tissues during initial loading. This study aimed to quantify hoof slip duration in retired Thoroughbred racehorses galloping over turf and artificial (Martin Collins Activ-Track) tracks at the British Racing School in the following four shoeing conditions: 1) aluminium; 2) steel; 3) G...
Competition and stereotypic behavior in Thoroughbred horses: The value of saliva as a diagnostic marker of stress.
PloS one    October 7, 2024   Volume 19, Issue 10 e0311697 doi: 10.1371/journal.pone.0311697
Bazzano M, Marchegiani A, La Gualana F, Petriti B, Petrucelli M, Accorroni L, Laus F.Many horses exhibit stereotypies, especially when living in human controlled environments that may prevent horses from satisfying natural needs in terms of feeding, drinking, moving, and socializing. In human medicine, obsessive compulsive disorder and other severe psychiatric disturbances are associated with stereotypic behaviors; salivary biomarkers evaluation is considered a reliable tool for diagnosis of common mental health disorders because saliva collection easy to obtain and noninvasive. In this study, we hypothesized that salivary cortisol concentrations, in addition to alpha-amylase ...
Lawsonia intracellularis regulates nuclear factor-κB signalling pathway during infection.
PloS one    September 26, 2024   Volume 19, Issue 9 e0310804 doi: 10.1371/journal.pone.0310804
Yang HW, Hu T, Ait-Ali T.Lawsonia intracellularis is the etiological agent of proliferative enteropathy (PE) in pigs, horses and wide range of mammals. Little is known about the role of innate immune response during L. intracellularis infection. In this study, we investigated the nuclear factor-κB (NF-κB)-regulated immune response against infection of a clinical strain Dkp23 and a live-attenuated Enterisol vaccine strain in PK-15 cells. We found that expression of NF-κB target genes TNF-α, IFN-γ, IL-6 and IL-8 were modulated during the course of infection. At 5 dpi, there was a significant increase in p65 NF-κB ...
Nineteen years of radiographic screening: Impact of sepsis and evolution of osteochondrosis dissecans prevalence in Walloon sport horses born between 2004 and 2022.
PloS one    September 10, 2024   Volume 19, Issue 9 e0308304 doi: 10.1371/journal.pone.0308304
Van Cauter R, Caudron I, Lejeune JP, Rousset A, Serteyn D.Osteochondrosis dissecans is a particularly common developmental orthopaedic disorder in equines. Among its causes, the hereditary component is no longer a matter of debate, and, for several decades, the various studbooks for sport horses have been selecting stallions in order to limit the prevalence of this condition in the population. However, to our knowledge, no study has evaluated changes in the prevalence of osteochondrosis dissecans over time through longitudinal monitoring of a population of sport horses. The study presented here is part of a large screening programme for developmental...
Association between equine asthma and fungal elements in the tracheal wash: An environment-matched case-control study.
PloS one    September 6, 2024   Volume 19, Issue 9 e0309835 doi: 10.1371/journal.pone.0309835
Dély S, Gerber V, Peters LM, Sage SE.The presence of fungi in tracheal wash (TW) of horses was recently linked to mild-moderate equine asthma, indicating a possible causal role; however, increased numbers of fungi may also stem from asthma-related alteration of tracheal mucus clearance or from environmental exposure. Our objective was to elucidate the association between the presence of fungi in TW and asthma status while controlling for relevant confounders. We conducted a retrospective case-control study involving 73 horses, including 34 controls and 39 asthmatic cases. Each asthmatic horse was matched with a control from the s...
Cortisol levels, heart rate, and autonomic responses in horses during repeated road transport with differently conditioned trucks in a tropical environment.
PloS one    September 6, 2024   Volume 19, Issue 9 e0301885 doi: 10.1371/journal.pone.0301885
Lertratanachai S, Poochipakorn C, Sanigavatee K, Huangsaksri O, Wonghanchao T, Charoenchanikran P, Lawsirirat C, Chanda M.Horse's stress responses have been reported during road transport in temperate but not tropical environments. Therefore, this study measured cortisol levels, heart rate (HR), and heart rate variability (HRV) in horses during medium-distance road transport with different truck conditions in a tropical environment. Six horses were repeatedly transported in either air-conditioned trucks with full (ATF) or space (ATS) loads or non-air-conditioned trucks with full (N-ATF) and space (N-ATS) loads. Blood cortisol was determined beforehand and 5, 30, and 90 minutes post-transport. HR and HRV were asse...