Analyze Diet

Topic:Nutrition

Nutrition in horses encompasses the study of dietary requirements and feeding practices that support equine health, growth, and performance. It involves the analysis of nutrients such as carbohydrates, proteins, fats, vitamins, and minerals, and their roles in equine physiology. Proper nutrition is essential for maintaining optimal body condition, supporting metabolic processes, and preventing dietary-related disorders. Research in this field examines the nutritional needs of horses at different life stages and activity levels, as well as the effects of various feed types and supplements. This page compiles peer-reviewed research studies and scholarly articles that explore the composition, digestion, and impact of different dietary components on equine health and performance.
Do Polish Konik Horses Find Soaked Hay to Be Palatable?
Animals : an open access journal from MDPI    May 29, 2026   Volume 16, Issue 11 1663 doi: 10.3390/ani16111663
Tkaczyk E, Gazda IE, Humięcka M, Łuszczyński J, Basińska K, Kaczmarek B, Barłowska J, Tkaczyk P, Janczarek I.Frequently, equine diseases necessitate the moistening or soaking of hay for the horses. It was assumed that this treatment would not reduce the willingness of the Polish Konik horses with low nutritional requirements to consume the hay. Furthermore, it was believed that moistening would increase the palatability of this feed. The aim of this study was to determine time and frequency parameters and the amount of unconsumed dry, moist, or soaked hay. The research material comprised 12 Polish Konik horses consuming either 1 kg of dry hay, 2.1 kg of moist hay, or 3.3 kg of soaked hay on three sub...
A Revised View on Equine Hypertriglyceridemia, Hyperlipidemia, and Hyperlipemia.
The Veterinary clinics of North America. Equine practice    May 28, 2026   S0749-0739(26)00022-2 doi: 10.1016/j.cveq.2026.04.007
Dunkel B.Hypertriglyceridemia, also known as hyperlipidemia or hyperlipemia, affects equids of all breeds and ages, mostly secondary to a negative energy balance triggered by a disease process. Increased triglyceride concentrations (TG) have been described in well-eating equids with endocrinopathies, atypical myopathy, severe liver disease, and after treatment with sodium-glucose transporter 2 inhibitors. The fact that equids can have substantially increased TG without showing clinical signs prompted reevaluation of the clinical effects of hypertriglyceridemia. As long as the consequences of high TG ar...
The Role of Omega-3 Fatty Acids in Horses’ Nutrition-A Review.
Animals : an open access journal from MDPI    May 27, 2026   Volume 16, Issue 11 1626 doi: 10.3390/ani16111626
This narrative review presents examples of omega-3 fatty acids supplementation in horse nutrition. Fats are essential in equine nutrition, serving not only as an energy source but also as functional components influencing health. Among them, omega-3 polyunsaturated fatty acids (PUFAs), particularly EPA and DHA, play a key role in modulating inflammatory and metabolic processes. This review summarizes their effects on respiratory health, equine metabolic syndrome (EMS), musculoskeletal function, reproduction, and early development. Omega-3 supplementation has been shown to support airway inflam...
Effects of Dietary Zinc Cysteamine Supplementation on Growth Performance, Physiological Responses, and Fecal Microbiota in Weaned Foals.
Animals : an open access journal from MDPI    May 21, 2026   Volume 16, Issue 10 1568 doi: 10.3390/ani16101568
Ren J, Ma C, Yang K, Li X, Yang F, Guo X, Yao X, Wang C.Zn-CS chelate has shown beneficial effects on gut health and growth in piglets, but its impact on weaned foals remains largely unknown. This study evaluated the effects of dietary Zn-CS supplementation on growth performance, nutrient digestibility, physiological status, and fecal microbiota in weaned Ili foals. Thirty-two six-month-old foals were randomly assigned to four treatment groups receiving 0, 2, 4, or 6 mg Zn-CS/kg body weight per day for 90 days. Growth performance, nutrient digestibility, plasma biochemical parameters, liver function enzymes, serum hormones, antioxidant indices, fec...
Effects of supplementing concentrates with different energy levels on growth performance, blood indicators, and fecal microbiota of Mongolian horses in late gestation.
BMC veterinary research    May 19, 2026   doi: 10.1186/s12917-026-05568-8
Liu Y, Wang X, Wang G, Wu Z, He Q, Zhao B, Du M, Zhao Y, Bou G, Bai D, Dugarjaviin M, Zhang X.Horse breeding and management have a globally extensive history, with horses serving vital roles in transportation, warfare, agriculture, and cultural activities. In China, particularly in Inner Mongolia, the horse industry faces challenges such as harsh winters and limited nutritional research for local breeds, especially during late gestation. This critical period is essential for fetal development and maternal health, and inadequate nutrition can lead to complications like nutritional abortions and poor foal growth. Similar issues exist worldwide in cold climates or regions with seasonal pa...
Alkaline-Promoted Hydrothermal Liquefaction of Polo Horse Manure for Biocrude Production.
ACS omega    May 16, 2026   Volume 11, Issue 21 31510-31520 doi: 10.1021/acsomega.6c01555
Namsiri P, Pianthong K, Thanakijkasem P.Horse manure (collected as pure feces) is a high-moisture, nutrient-dense organic waste stream with potential for valorization via hydrothermal liquefaction (HTL). However, catalyst selection is often reported without direct catalyst-class comparisons under identical conditions. In this study, manure collected in Thailand was characterized and converted via noncatalytic and catalytic HTL in a batch reactor at 300 °C for 40 min (feedstock/water = 1:10, w/w; heating rate 10 °C min). Four catalysts (KOH, NaOH, HPO, NH·HO) were evaluated at 10 and 20 wt % (dry feed basis), with biocrude recover...
Mitochondrial dysfunction in equine ageing: Current evidence and clinical implications for geroscience.
Equine veterinary journal    May 11, 2026   doi: 10.1002/evj.70189
Puchalska M, Witkowska-Piłaszewicz O.Ageing is a complex biological process affecting a growing geriatric human and equine population worldwide. Mitochondrial dysfunction is considered one of the hallmarks of ageing, representing a multifaceted process. This review synthesises current findings on mitochondrial dysfunction in aged equines, drawing parallels with human medicine and identifying current knowledge gaps. Integrating findings from human and equine research may bridge existing challenges and offer new opportunities, including the development of novel translational models for ageing research. Furthermore, it highlights th...
Long-Read Sequencing for Species-Level Resolution of the Equine Gut Microbiota Reveals the Need for Improved Databases.
Animals : an open access journal from MDPI    May 9, 2026   Volume 16, Issue 10 1459 doi: 10.3390/ani16101459
Leduc L, Boucher L, Mach N, Leclère M, Costa M.Differences in gut microbiota composition related to diet have been reported in horses, but characterization of specific microbial taxa remains limited, particularly at the species level. The objective of this study was to use long-read sequencing of the 16S rRNA gene to provide additional taxonomic insight into the intestinal microbiota in horses. Fecal samples were collected from 12 horses on pasture and from 6 of them after switching to a hay diet. Sequencing yielded low read counts per sample, and the analysis failed to detect statistical differences in alpha- and beta-diversity among diet...
The Effects of Boron Mineral on Performance, Bone and Mineral Metabolism in Purebred Arabian Foals.
Biological trace element research    May 6, 2026   doi: 10.1007/s12011-026-05138-x
Yilmaz İÇ, Güler T, Ekmen EG, Özçelik M.Boron plays significant roles in various biological systems, including mineral, lipid, and energy metabolism, immune and endocrine systems, and brain function. It has been suggested to enhance performance and may prevent conditions such as osteoporosis, osteoarthritis, and arthritis. Despite these known benefits, its effects on growth performance and mineral metabolism in horses remain understudied, particularly in young animals like Purebred Arabian foals. The primary objective of this study was to investigate the effects of different doses of boron supplementation (0, 5, 10, and 15 mg/day ...
Oral docosahexaenoic acid supplementation alters alveolar macrophage metabolism and protein and lipid profiles in the lower airway of healthy horses.
American journal of veterinary research    May 4, 2026   1-9 doi: 10.2460/ajvr.26.01.0001
Cooper BL, O'Donnell K, Hickman E, Bayless R, Sheats MK.To determine the impact of a commercially available docosahexaeonic acid (DHA) supplement (DHA, methylslfonylmethane, and mushroom blend) on equine alveolar macrophage metabolism and lipid and protein profiles in bronchoalveolar lavage (BAL) supernatant. Unassigned: This was a prospective, sequential, placebo-controlled study using 10 healthy adult horses. Bronchoalveolar lavage fluid was collected at baseline, following 45 days of oral placebo administration, and following 45 days of oral commercially available DHA supplement, with a 14-day washout. Whole blood was collected following placebo...
The effect of form on equine salt intake.
Journal of equine veterinary science    May 2, 2026   105923 doi: 10.1016/j.jevs.2026.105923
Murphy B, Catalano DN.Sodium and chloride are required nutrients and are typically met using sodium chloride (salt) via compressed blocks. The objective of this study was to compare salt intake using block or loose salt. Sixteen mature horses were blocked by housing style and assigned to block salt or loose salt (form) for four 7-day periods. Salt intake was measured by weight at the end of each period and was not affected by form (P = 0.070) or period (P = 0.902). There was an effect of horse (P < 0.001). Horses consumed between 2 and 135 g of salt daily, however; over half of the horses in the study di...
Accuracy of large language model-based artificial intelligence tools for equine topics.
Journal of equine veterinary science    May 1, 2026   105924 doi: 10.1016/j.jevs.2026.105924
Aldworth-Yang S, Coleman SJ, O'Reilly K, Catalano DN.Artificial intelligence (AI) platforms are becoming increasingly popular as resources for equine information. However, these platforms generate responses from a wide range of sources and do not always distinguish between fact and opinion. Objective: The objective of this study was to assess the accuracy and quality of AI-generated answers to equine-related questions. Researchers hypothesized that AI platforms could answer basic equine questions effectively but would perform poorly on complex topics or questions. Methods: Forty questions were written covering general horse care, facilities mana...
The Effects of L-Citrulline on the Apparent Digestion and Metabolism of Nutrients, Blood Hormone Levels, Amino Acid Metabolism and the Diversity of Faecal Microbiota in Mares in the Later Stage of Pregnancy.
Life (Basel, Switzerland)    April 30, 2026   Volume 16, Issue 5 744 doi: 10.3390/life16050744
Liu P, Yang F, Li J, Li C, Guo X, Li X.This study aimed to investigate the impact of dietary L-citrulline supplementation on the health of mares during late gestation. Thirty-two healthy mares in late pregnancy were randomly assigned to four groups: a control group (CON, 0 g/d) and three treatment groups receiving 15, 30, and 45 g/d/head of L-citrulline, respectively. The trial spanned 72 days, including a 12-day adaptation phase and a 60-day formal feeding period. A fixed daily feeding amount of 11.2 kg/head was provided, ensuring complete consumption and consistent dry matter intake across all groups. Results demonstrated that su...
Pasture Intake of Whole-Day Stocked Thoroughbred Foals With Growth.
Animal science journal = Nihon chikusan Gakkaiho    April 28, 2026   Volume 97, Issue 1 e70168 doi: 10.1111/asj.70168
Tanabe T, Matsui A, Tominari M, Ueda K, Mitani T, Kawai M.We investigated the changes in the forage and nutrient intake of thoroughbred foals on pastures with whole-day stocking with age. Twelve thoroughbred foals were used and were allowed to graze on a pasture for 21 h/day from June to September. All foals were weaned just before 5 months of age. The pasture intake of the foals was measured using the double-indicator method. During the suckling period, the total voluntary dry matter intake (VDMI) of the pasture for foals was higher at 4 months (2.1 kgDM/day) than at 2 months (1.3 kgDM/day). However, VDMI of pastures per body weight (%BW) for foal...
Efficacy of an iron absorption test on assessing iron status in horses.
Journal of equine veterinary science    April 23, 2026   105913 doi: 10.1016/j.jevs.2026.105913
Gluck-Flynn CR, Pratt-Phillips SE.Iron absorption tests (IATs) are used to evaluate iron absorption in humans. However, this testing has not been conducted in horses. Objective: The objective was to assess how a large dose of iron affects blood iron parameters during an IAT. Methods: Mixed-breed geldings (n=12) were offered 2% of their bodyweight (BW) in mixed-grass hay and an iron-free vitamin-mineral supplement. A baseline IAT (Baseline IAT) was performed, where horses received 25 milligrams per kilogram (mg/kg) of BW of iron (ferrous sulfate) orally. Jugular venous samples were taken before the iron dose, and 3-, 6-, 12-, a...
Downregulation of a choline transporter gene in equine colitis indicates a role for choline supplementation to support the large intestinal epithelial barrier.
Journal of equine veterinary science    April 23, 2026   105916 doi: 10.1016/j.jevs.2026.105916
Sheahan BJ, O'Neill MK, Jeter MA, McDermott LB, Megeed HS.Acute colitis is an inflammatory condition of the equine colon associated with high morbidity and mortality. In these horses, epithelial barrier function is critical but the mechanisms underlying large intestinal epithelial damage and recovery are incompletely understood. Objective: Our objectives were to (1) identify intestinal colitis-associated transcriptional pathways, and (2) determine the effect of in vitro choline supplementation on the epithelial barrier. Methods: We performed RNA-sequencing on rectal biopsies from healthy and colitis horses (n=3/group). Downregulated SLC5A7, a gene en...
Evaluation of the tolerance and effects of 1-deoxynojirimycin on insulin and glucose dynamics in healthy horses and horses at risk for insulin dysregulation.
Journal of veterinary internal medicine    April 21, 2026   Volume 40, Issue 2 aalag074 doi: 10.1093/jvimsj/aalag074
Whitfield-Cargile C, Coleman M, Hart K, Gomes D, Berghaus L, Duberstein KJ, Ellis K, Tinkle A, Shirzad R.Insulin dysregulation (ID) is common and diminishes welfare of horses. Current management relies on diet and exercise, with variable responses and limited medical options. Alpha-glucosidase inhibitors (AGIs) might provide adjunctive therapy. Objective: To evaluate the tolerance and effects of Reducose®, a Morus alba leaf extract standardized to 5% 1-deoxynojirimycin (DNJ), in healthy horses and horses at risk of ID. We hypothesized DNJ would be well-tolerated and reduce insulin responses to oral sugar tests (OSTs). Methods: Twenty-seven horses: 6 healthy (Phase I), 5 at risk for ID (Phase II)...
Successful nutritional management of a warmblood horse with multiple pathologies that respond to nutrition.
Journal of equine veterinary science    April 21, 2026   105908 doi: 10.1016/j.jevs.2026.105908
López-Císcar C, Ibáñez-López A, Rivero JLL, Harris P.This report describes the nutritional management of an 18-year-old, 553 kg gelding warmblood used for dressage lessons that presented with severe weight loss, marked muscle atrophy, poor dentition, sabulous urolithiasis, gastric ulcers, and later small colon impaction. On admission, the diet consisted of ad libitum wheat straw and mixed cereals (73:27 forage:concentrate), providing 2% of body weight as dry matter without vitamin-mineral supplementation. Marked deficiencies were identified in digestible energy (38%), crude protein (20%), lysine, threonine, vitamins, and minerals, while sugar an...
Equine Oocyte and Embryo Metabolism in the ART Setting: Current Knowledge and Clinical Significance.
Journal of equine veterinary science    April 21, 2026   105910 doi: 10.1016/j.jevs.2026.105910
Lewis N.Equine in vitro embryo production has become very efficient and is used extensively worldwide for the clinical production of foals. What we know so far about offspring health and performance is promising; however, elements of the in vitro process remain unphysiological when compared to in vivo conditions. Studying the metabolism of oocytes and embryos can help inform the optimisation of culture systems and identify aberrations if they occur. Given the growing body of knowledge on the downstream effects of an inadequate peri-conception environment, both in vivo and in vitro, it is imperative th...
Post exercise hydration responses to an electrolyte, glycerol, and creatine supplement in horses: a preliminary study.
BMC veterinary research    April 20, 2026   doi: 10.1186/s12917-026-05493-w
Peterson R, Dietrich J.Electrolyte supplementation is utilized to support hydration in horses. However, limited data are available on formulations that include osmotically active compounds such as glycerol and creatine. These additional ingredients may enhance fluid retention, yet their physiologic effects in equine athletes have not been evaluated. This preliminary study assessed hematologic responses, body mass recovery, and voluntary water intake following moderate exercise with and without supplementation using a commercially available hydration supplement containing electrolytes, creatine, and glycerol. Six hea...
Milk productivity of mares.
Brazilian journal of biology = Revista brasleira de biologia    April 20, 2026   Volume 86 e303508 doi: 10.1590/1519-6984.303508
Asset Z, Rahim K, Kargayeva M.To determine the milk productivity of Kazakh horses of the Adai region in variants of selection and technological indicators of milk for the production of cumus in the conditions of the Mangyshlak peninsula of the Republic of Kazakhstan. The object of the study was the Kazakh horse of the Adai intra-breed type at "Taushyk" LLP (Tupkaragan District, Mangystau Region). Mares with a more pronounced milk production were selected and analyzed . they have investigated the quality of milk for further processing. A characteristic feature of the dynamics of milk sugar content during lactation has been ...
Exploratory evaluation of equine placental extract supplementation on ageing indicators in geriatric dogs: a single-arm pre-post study.
BMC veterinary research    April 15, 2026   Volume 22, Issue 1 311 doi: 10.1186/s12917-026-05475-y
Morita N, Hirano E.BACKGROUND: Recent advances in preventive veterinary healthcare, nutritional management, and owner engagement have extended the longevity of domestic dogs. However, this increased lifespan has led to a higher prevalence of age-associated physiological and functional decline. Equine placental extract contains a variety of bioactive constituents, with reported antioxidative and anti-inflammatory properties. Although the use of equine placental extract in veterinary practice is expanding, empirical evidence supporting its effects on ageing-related clinical outcomes in dogs remains limited. In thi...
Overview of the Current Osteoarthritis Treatment in Veterinary Medicine and Future Directions.
ACS pharmacology & translational science    April 14, 2026   Volume 9, Issue 5 1037-1054 doi: 10.1021/acsptsci.5c00706
Quintão NLM, Moffa EB, Kroier M, Goldoni RC, Bundgaard L, Jensen AL, Fernandes ES.Osteoarthritis (OA) is a chronic and progressive joint disease that can cause permanent loss of joint function. OA is highly prevalent in horses, dogs, and cats, affecting different joints and leading to reduced mobility and chronic pain. Different risk factors for animal OA have been identified, including trauma and breed. Inflammation is a hallmark of the disease, with various underlying mechanisms such as cyclooxygenase and metalloproteinase activation, nerve growth factor, cytokine, and prostaglandin upregulation, among others. Although disease complexity makes OA difficult to treat, eno...
Microbiome analysis reveals the positive effects of exercise on the gut microbiota in horse.
Microbial pathogenesis    April 13, 2026   108501 doi: 10.1016/j.micpath.2026.108501
Ma R, Wang C.Gut microbiota is vital to host health, involving many aspects including immunity, metabolism and digestion. Exercise is recognized as a significant factor influencing the gut microbiota. However, the effects of exercise on the gut microbiota in horses remain unclear. Here, this study aims to investigate the effects of exercise on the gut bacterial and fungal communities in the horses using 16S rDNA and ITS genes amplicon sequencing. Results indicated that exercise significantly increased the ACE, Chao1 and Shannon indices of the gut bacterial community, accompanied by significant alterations ...
Terpenic compounds possess anthelmintic and immunomodulatory properties with potential for controlling equine cyathostomin infections.
International journal for parasitology. Drugs and drug resistance    April 12, 2026   Volume 31 100642 doi: 10.1016/j.ijpddr.2026.100642
Malsa J, Chereau A, Guégnard F, Serreau D, Gesbert A, Reigner F, Chamoin L, Guillot J, Vernudachi A, Mach N, Fleurance G, Lacroix-Lamandé S....The emergence of anthelmintic-resistant parasite isolates necessitates alternative control strategies. The use of dietary additives with anti-parasitic or immunomodulatory activities has been proposed as a solution, but whether such additives can simultaneously exert dual bioactivities within this context has rarely been explored. Here, we evaluated whether selected terpenes could exert dual bioactivities (anti-parasitic and immunomodulatory) with a view to their use as food additives that can limit strongyle infections in horses. In vitro tests with cyathostomin larval development, larval mig...
Survey of equine pasture best management practices and soil fertility in southeast Pennsylvania after the grazing season.
Journal of equine veterinary science    April 11, 2026   105893 doi: 10.1016/j.jevs.2026.105893
Splan RK, Noel FI, Gardner GM, Kenny LB.Proper pasture management improves forage yield, reduces costs, and minimizes environmental impacts, but may be difficult in suburban settings. Objective: This study evaluated pasture condition and management practices on 28 horse farms in counties adjacent to the Philadelphia metropolitan area. Methods: Between November 2019 and January 2020, 72 pastures (2-3/farm) were assessed via soil sampling, USDA-NRCS Pasture Condition Score Sheet (PCSS), Penn State Equine Pasture Evaluation Disc (EPED), and farm manager interviews. Linear models assessed effects of management factors on pasture conditi...
An overview of the livestock microbiome: sheep, horses, cattle, camels, and chickens.
Brazilian journal of biology = Revista brasleira de biologia    April 10, 2026   Volume 86 e299936 doi: 10.1590/1519-6984.299936
The animal microbiome plays a crucial role in determining the health, productivity, and welfare of livestock species, including sheep, horses, cattle, camel, and chicken. These animal species were selected due to the high consumption of their products in Kazakhstan. Enhancing their productivity, while maintaining the safety and quality of meat and milk derived from them, represents a pressing research priority. This review article includes current research on the composition, diversity, and purposes of the microbiota found within different organ systems of these species. This study focuses on ...
Physiological and nutritional adaptation of broodmares during the transition from late pregnancy to early lactation: Digestibility changes and the role of uNDF as an internal marker.
Veterinary research communications    April 9, 2026   Volume 50, Issue 4 258 doi: 10.1007/s11259-026-11194-4
Lamanna M, Mariella J, Freccero F, Lanci A, Colleluori R, Ghiaccio F, Buonaiuto G, Valle E, Raspa F, Castagnetti C, Cavallini D.Nutritional management of broodmares during late pregnancy and early lactation is essential for maternal health and foal well-being. This study evaluated a targeted dietary adaptation during the transition from late gestation to early lactation, monitored nutrient digestibility, and applied undigested neutral detergent fiber (uNDF) as an intrinsic digestibility marker. A prospective nutritional study was conducted on 11 clinically healthy Trotter broodmares (3 primiparous, 8 multiparous) monitored under standardized conditions. Mares received a forage-based diet with controlled starch intake a...
Polyphenol-Rich Feed Material increases Anaerostipes and reduces methanogenic archaea in the horse hindgut microbiome.
Journal of equine veterinary science    April 4, 2026   105885 doi: 10.1016/j.jevs.2026.105885
Bettio MK, Vidal T, Rose JJ, Jois M, Flavel M, Petrovski S.The gastrointestinal microbiota plays a critical role in horse health and performance. While sugarcane-derived polyphenols have shown microbiota-modulating properties in other species, their effects in horses remain unexplored. Objective: This study investigated whether supplementation with a sugarcane-derived polyphenol feed material modulates the hindgut microbiota of healthy adult horses. Methods: An observational longitudinal study was conducted on six horses over 12 weeks. Faecal samples were collected at three time points: baseline (P1), during supplementation (P2), and post-supplementat...
Biomarkers of Adipose Color: A Multi-Omics Analysis Unravels the Molecular Landscape of White and Yellow Fat in Kazakh Horse.
Biology    April 1, 2026   Volume 15, Issue 7 563 doi: 10.3390/biology15070563
Chang X, Shi X, Luo P, Yao X, Meng J, Wang J, Ren W, Li L, Zeng Y.Fat color is one of the most important economic traits influencing consumer preference and the market value of meat products. To explore the molecular mechanisms underlying abdominal adipose tissue color differentiation in the Kazakh horse, this study employed a multi-omics strategy, integrating targeted metabolomics (fatty acid and amino acid profiling), untargeted metabolomics, and transcriptomic analyses. Two types of adipose tissue, white adipose tissue (WAT, = 8) and yellow adipose tissue (YAT, = 8), were selected for systematic comparative analysis. Fatty acid composition analysis reve...