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Topic:Young Horses

Young horses are generally defined as those under five years of age, transitioning from foals to adults. Research in this area examines the study of equine development, behavior, and health across different life stages. This includes the physiological and anatomical changes that occur as horses mature from foals into adults. Additionally, understanding behavioral aspects such as socialization, learning, and adaptation to training is key to this field. This page gathers peer-reviewed research studies and scholarly articles that explore the developmental processes, health considerations, and management practices relevant to young horses and their transition into adulthood.
New Noninvasive Long-Term Stress indicator in Horses: Analysis of Cortisol Levels in the Hoof of Foals.
Animal science journal = Nihon chikusan Gakkaiho    October 16, 2026   Volume 97, Issue 1 e70251 doi: 10.1111/asj.70251
Ojima Y, Kimura Y, Maeda Y, Matsuura A.Noninvasive sampling methods are recommended in animals as far as possible. However, there has been no way to safely measure long-term stress in all horses including foals and stallions. Currently, nail cortisol in humans has been reported to be a promising noninvasive long-term stress indicator. This study aimed to evaluate the usefulness of a horse's hoof as a noninvasive long-term stress indicator by determining whether hoof cortisol levels varied similarly to hair cortisol levels noted in a previous study. All hooves were sampled by one farrier after a foal's birth. They were divided into ...
Ultrasonography of the spleen, kidneys and bladder in healthy Mangalarga Marchador horses across different life stages.
Journal of equine veterinary science    October 6, 2026   106170 doi: 10.1016/j.jevs.2026.106170
Pessoa GO, Botelho JHV, Junior ACCL, Ferrante M, Sousa TM, Peconick AP.This study is the first breed-specific ultrasonographic reference values for spleen, kidneys, and bladder in Mangalarga Marchador horses from different life stages. Objective: The aim of this study was to establish a normal reference range of measurements of the spleen, kidneys and bladder, as well as to describe the anatomy and sonographic characteristics of these organs in Mangalarga Marchador horses. Methods: Abdominal ultrasound examinations were performed in 88 Mangalarga Marchador horses of both sexes, aged 1, 7 and 15 days, monthly from their 1st to the 11th month of age ando n their 1s...
The effects of feeding frequency on glycemia and insulinemia in moderately exercised young horses.
Research in veterinary science    October 5, 2026   Volume 212 106428 doi: 10.1016/j.rvsc.2026.106428
Hess T, da Costa Santos H, Black J.Concentrates are typically fed twice daily to supplement forages in horses to facilitate management, especially those with higher energy needs, but high-grain meals can contribute to digestive and metabolic challenges, including insulin dysregulation. This study investigated the effects of feeding frequency on glycemia, insulinemia, and insulin sensitivity measured by an oral sugar test in moderately exercised young horses. Thirty-two healthy Quarter Horses (2-3 years old) were assigned to either traditional feeding (TF; two grain meals/day) or fractioned feeding (FF; automated grain delivery...
Phenotypic associations between young horse assessments and orthopaedic diagnoses in insured Swedish Warmblood horses.
Equine veterinary journal    September 23, 2026   doi: 10.1002/evj.70331
Bonow S, Hernlund E, Eriksson S, Strandberg E, Viklund ÅG.Lameness is the most common health problem in riding horses, and insurance data have previously been used to investigate the prevalence of orthopaedic diagnoses in Swedish Warmblood (SWB) horses. Young horse performance testing is an important part of the SWB breeding programme and contributes data to the genetic evaluation. Assessed traits from these tests have been shown to be useful indicator traits of performance in show jumping and dressage, but their associations with orthopaedic health have not previously been investigated. Objective: To study the associations between assessed traits fr...
Behavioral changes in thoroughbred horses following pre-training: insights from novel object tests and activity monitoring under field conditions.
Frontiers in veterinary science    September 21, 2026   Volume 13 1927282 doi: 10.3389/fvets.2026.1927282
Pinetzki L, Menze J, Eichler F, Brüsewitz I, Engler T, Stutzke S, Merle R, Hoffmann G, Wiegard M, Thöne-Reineke C, Kirsch K.Racehorse training starts with pre-training, which is conducted at a very early age and remains controversial from an animal welfare perspective. This study investigated how a 12-week pre-training program influenced the behavior of young Thoroughbred horses. Horses were assessed before (A) and after (B) pre-training, which progressed from habituation and handling to ridden exercise and canter work on a racetrack. Behavioral responses were evaluated using standardized novel object tests and continuous 24-h stall monitoring via inertial measurement unit sensors. Novel object test behavior was vi...
Oxidative stress markers in different age ranges of healthy horses.
Research in veterinary science    September 20, 2026   Volume 212 106415 doi: 10.1016/j.rvsc.2026.106415
de Brito Souza LMR, Carrasco LMC, Vasconcelos LA, de Campos Lopes LE, Bosculo MRM, Montechiesi DF, de Almeida BFM.Oxidative stress results from an imbalance between oxidants and antioxidants, impairing cellular functions and contributing to diseases and aging. Despite the growing interest in oxidative stress, studies specifically evaluating the influence of age on laboratory and oxidative stress parameters in clinically healthy horses remain limited. Thus, this study aimed to evaluate the influence of age on laboratory and oxidative stress parameters in clinically healthy horses. A total of 152 clinically healthy horses (80 females and 72 males) were selected, including young horses aged 3 to 48 months (...
Training- and competition-related changes on circulating adrenocorticotropic hormone and cortisol response in Thoroughbred Racehorses.
Journal of equine veterinary science    September 4, 2026   106137 doi: 10.1016/j.jevs.2026.106137
Cravana C, Fazio E, Satué K, Brancato G, Deborah F.Exercise is a physiological challenge inducing intensity-dependent hypothalamic-pituitary-adrenal (HPA) axis responses. The hypothesis was that training and racing sessions induce different HPA axis activation. Objective: To assess whether different exercise sessions affect equine adrenal responsiveness according to age and sex. Methods: Ten clinically healthy Thoroughbred racehorses (6 females and 4 males) were recruited. Four were 2 years old and six were 3 years old. Horses were subjected to two exercise conditions: training and competitive racing sessions. Blood samples were collected at r...
Hidden Tarsal Lesions: The Role of Flexed and Oblique Radiographs in Young Horses.
Journal of equine veterinary science    August 28, 2026   106136 doi: 10.1016/j.jevs.2026.106136
Cianci JM, Dobbs EC, Bills KW, Engiles JB, Orsini JA.Radiographically apparent tarsal lesions associated with developmental orthopedic disease and joint sepsis are common in young horses. Standard radiographic projections are routinely used to identify osseous changes but may fail to detect lesions due to superimposition and complex anatomy. This retrospective case series describes plantar trochlear ridge lesions in young horses that were not identified on standard four projection radiograph series but were detected using flexed lateromedial and flexed oblique projections. Cases were selected based on persistent clinical signs localizing to the ...
Depth and distribution of colonic sand accumulation in a desert population of horses and its association with diagnosis of sand colic.
Journal of the American Veterinary Medical Association    August 21, 2026   1-6 doi: 10.2460/javma.26.02.0122
Sikorska Z, Stapley E, Oliveira J, Lazala JE, Fernandes T, David F, Vinardell T, Jamieson C.To describe patient and management characteristics associated with radiographic sand accumulation in a desert population of horses, as well as identify factors independently associated with a diagnosis of sand colic and need for surgical intervention. Unassigned: Horses were prospectively allocated into 3 groups: control horses, horses with sand colic, and horses with non-sand-associated colic. Signalment, radiographs, and management outcomes were compared between groups. Unassigned: Median depth of sand was significantly greater in horses diagnosed with sand colic compared to other groups (11...
Prevalence, distribution, and associated risk factors of major equine endoparasitic infections in South Gondar Zone, Northwest Ethiopia.
Veterinary and animal science    August 17, 2026   Volume 34 100821 doi: 10.1016/j.vas.2026.100821
Alemayehu H, Alemneh T, Birhan M, Seyoum Z, Baylie M.Endoparasites remain major constraints on equine health and productivity in Ethiopia; however, epidemiological data from northwestern regions are limited. This study investigated the occurrence, parasite diversity, infection intensity, and risk factors associated with endoparasite infections in equines in northwestern Ethiopia. A cross-sectional study was conducted using coprological examinations complemented by postmortem inspections. Standard parasitological techniques were employed, and multivariable logistic regression analysis was used to identify predictors of infection. Data were analyz...
Sleep architecture and lying behavior in horses across age groups and two housing systems: an overnight non-invasive EEG study.
Frontiers in veterinary science    August 6, 2026   Volume 13 1898580 doi: 10.3389/fvets.2026.1898580
Zimmer L, Schramel JP, Lyrakis M, Trachsel DS, Auer U, Leschnik M, Macho-Maschler S, Hotz M, Wahl H, Brunner S, Palme R, Cavalleri JV.Sleep is a crucial factor for the well-being of horses, yet assessing equine sleep in common housing conditions remains challenging. This study investigated the effects of changes in horses' immediate environment and the influence of age on their sleep and resting behavior using a novel, non-invasive electroencephalography (EEG) mask. Ten horses from the teaching herd at the University of Veterinary Medicine Vienna were observed over four consecutive nights in two different housing systems (a large communal stable vs. two separate, adjacent individual stalls). Data collection included EEG reco...
Hypothalamic-pituitary-adrenal axis response and glucose homeostasis during exercise to fatigue before and after training in old and young adult Standardbred mares.
Journal of equine veterinary science    July 28, 2026   106103 doi: 10.1016/j.jevs.2026.106103
Liburt NR, Herbst AC, Malinowski K, McKeever KH.While reduced cortisol responses to exercise have been described in older horses, the effects of aging and training on the hypothalamic-pituitary-adrenal axis (HPA axis) and downstream metabolic signaling are currently unknown. Unassigned: This study tested the hypothesis that age and exercise training alter the response of the HPA axis, insulin, and glucose during an acute exercise challenge to fatigue. Methods: Six old (22.0±0.7 years) and six young adult (7.3±0.6 years) unfit Standardbred mares were tested. Mares ran a graded exercise test before (GXT1) and after (GXT2) 8 weeks of trainin...
Environmental and genetic effects on measured hock bone proportions in young Polish Warmblood horses undergoing performance test training for breeding horses.
Veterinary journal (London, England : 1997)    July 25, 2026   106795 doi: 10.1016/j.tvjl.2026.106795
Buczkowska R, Hecold M, Verwilghen D, Lewczuk D.The hock bone proportions in young Polish Warmblood horses (69) were studied based on x-ray image measurements done during official breeding horse performance testing. Six measurements were used to identify 4 width and length proportion indices. The radiological examinations were done before and after horse training, which consists of work with or without the rider at walk, trot and canter (40-50min) with some light class (< 100cm) jumping (<12 jumps) 6 days a week. The training effect was investigated using analysis of variance (procedure MIXED, Statistical Analysis System). The statist...
The Peridental Structures of Equine Cheek Teeth: An Age-Dependent Anatomical Study of Maxilla and Mandible.
Animals : an open access journal from MDPI    July 18, 2026   Volume 16, Issue 14 2225 doi: 10.3390/ani16142225
Fewson S, Lüpke M, Hellige M, Seifert H, Bienert-Zeit A, Staszyk C.Sequestration is the most common complication following equine cheek tooth extraction, occurring predominantly in the mandible of younger horses. Extraction-related trauma to the peridental region may impair healing. Despite its clinical relevance, detailed knowledge of peridental anatomy remains limited. This study investigated age-related differences in the peridental region of maxillary and mandibular equine cheek teeth 07 to 09. The peridental region of 24 cheek teeth rows from six cadaver heads was sectioned horizontally at approximately 10 mm intervals. Sections were photographed and the...
Imaging Diagnosis: Imaging Diagnosis of Lateral Plantar Digital Vein Thrombosis in a Yearling Thoroughbred. Garcia-Collao MR, Sanclemente JL, Larriva DS, Roberts GD, Ragle CA.A 1-year-old thoroughbred filly was referred to the Washington State Veterinary Teaching Hospital with a 4-month history of left hind limb swelling, from the mid metatarsus to the distal pastern, without lameness. Previous imaging (digital radiographs, ultrasound, and low-field magnetic resonance imaging [MRI]) was inconclusive, with a tentative diagnosis of lymphatic drainage impairment. A high-field MRI diagnosed left lateral plantar digital vein thrombosis, with secondary lymphatic dilation and edema. The filly was discharged with rehabilitation instructions and resumed exercise shortly aft...
Horses without ocular disease maintain similar electroretinographic responses and peripapillary retinal thicknesses across age groups.
American journal of veterinary research    July 7, 2026   1-7 doi: 10.2460/ajvr.26.03.0141
Gray AP, Ledbetter EC, Thirunagari N, Irias-Valvo K, Knickelbein KE.To determine the impact of age on equine electroretinography (ERG) parameters and retinal thickness in horses with healthy eyes. We hypothesized that ERG b-wave amplitudes would be significantly lower in older horses and that total retinal thickness would not vary significantly with age. Unassigned: Horses with clinically normal eyes comprising 3 age groups (≤ 10, 11 to 20, and ≥ 21 years) underwent scotopic and photopic ERGs, high-resolution fundus photography, and spectral-domain optical coherence tomography. Electroretinography results were compared between age groups using mean implici...
Short-term provision of moderate dietary starch alters fecal microbiota but does not exacerbate exercise-induced inflammation in yearling Quarter Horses.
Journal of equine veterinary science    June 27, 2026   106071 doi: 10.1016/j.jevs.2026.106071
DiSilvestro AN, Wesolowski LT, Williams BD, Warren LK, Athrey G, White-Springer SH.Energy-dense feeds commonly provided to equine athletes may be high in starch, which alter gastrointestinal microbiota and could promote systemic inflammation. Objective: To test the hypothesis that exercise-induced inflammation would be greater in horses receiving a starch- versus fiber-based concentrate. Methods: Quarter Horses (mean±SD 16±1mo; 337±30kg) received either a fiber-based control (CON; 7 fillies, 8 geldings) or an isocaloric, isonitrogenous starch concentrate (STARCH; 8 fillies, 7 geldings) for 24d. Fecal metagenomics were evaluated on d0 and 21. Blood inflammatory mediators w...
The progression values of heart rate and biochemical plasma parameters during training of 3-years old warmblood stallions for breeding licensing.
Journal of equine veterinary science    June 19, 2026   106062 doi: 10.1016/j.jevs.2026.106062
Dockalová H, Zalesakova D, Batik DB, Batik A, Kacetlova D, Horky P.Physiological adaptation to training in young sport horses remains incompletely characterized, particularly during standardized preparation for breeding licensing. Objective: The aim of this study was to evaluate cardiovascular and plasma biochemical responses to acute exercise and long-term training in three-year-old Czech Warmblood stallions during standardized free jumping exercise. Methods: Thirteen Czech Warmblood stallions were monitored over a 112-day training period. Heart rate parameters (minimum, maximum, and mean) were recorded during standardized free jumping tests. Plasma biochemi...
Polygenic Prediction of Equestrian Sport Discipline Among Horses Bred for Jumping and Dressage.
Animal genetics    June 18, 2026   Volume 57, Issue 3 e70146 doi: 10.1002/age.70146
Hill EW, Han H, Holtby AR, Anhold H, McGivney BA.Horses bred for different equestrian sports vary in physical, physiological and behavioural requirements. Characterising genetic markers associated with discipline provides an opportunity to improve identification of horses best suited to either jumping or dressage. Using a composite selection signals test, genomic regions under positive selection in German warmblood horses divergent for discipline were identified that contained lead SNPs proximal to or within genes with functions relevant to the requirements for jumping and dressage. Dressage genes function in tortuosity of movement (GRM5) an...
Sport predispositions in warmblood horses: insights from Grand Prix competitions.
Journal of equine science    June 13, 2026   Volume 37, Issue 2 53-60 doi: 10.1294/jes.37.53
Próchniak T.The aim of this study was to pilot the assessment of sport predispositions in warmblood horse breeds, both domestic and foreign, and to evaluate factors influencing their performance. Data from 293 horses (725 starts) competing in national Grand Prix events with obstacle heights of 140-145 cm were analysed using general mixed models. The origins of Polish Halfbred Horses were examined, and correlations between results in the Young Horse Championships and later Grand Prix performance were assessed. Results showed that performance is significantly influenced by genetic factors, such as breed and...
Impact of ununited plantar eminence of the first phalanx on early racing performance in North American Standardbreds.
Veterinary surgery : VS    June 10, 2026   doi: 10.1111/vsu.70121
Graczyk Z, Kapraun P, McCoy AM.To determine the prevalence of ununited plantar eminence (UPE) of the first phalanx (P1) in North American Standardbreds and evaluate its association with early racing performance. Methods: Retrospective cohort study with matched sibling controls. Methods: Imaging records from 1897 yearling Standardbred horses. Methods: Screening images were reviewed for the presence of UPE and other orthopedic abnormalities. Sex, age, sire, gait, and racing performance data were obtained from the United States Trotting Association. Affected horses were compared with unaffected siblings using non-parametric te...
Impact of collagen supplementation on the fecal microbiome of young horses in training.
Journal of equine veterinary science    June 6, 2026   106047 doi: 10.1016/j.jevs.2026.106047
Júnior AMCA, da Silva AH, Buroxid RP, Padovan JR, Nunes AT, Poleti MD, Fukumasu H, Gobesso AAO.Intense training in athletic foals may alter fecal microbiota, highlighting the need for nutritional strategies such as hydrolyzed collagen supplementation. Objective: This study aimed to evaluate the effects of dietary hydrolyzed collagen on the fecal microbiome of weanlings in training. Methods: Twenty Mangalarga Marchador foals (206 ± 18 kg; 7 ± 1 mo age) were assigned to two groups: a supplemented group (50 g/day of hydrolyzed collagen for 180 days) and a control group. A completely randomized design with repeated measures over time was used. Foals were exercised five consecutive days pe...
Validation of measurable radiographic parameters for quantitative assessment of carpal and metacarpal bone morphology in foals.
Veterinary journal (London, England : 1997)    June 5, 2026   Volume 318 106736 doi: 10.1016/j.tvjl.2026.106736
Son JK, De Paz P, Kim J, Sanaei R, Ryu S, Bailey S, Davies HMS.Radiographic morphometry provides a quantitative means to evaluate bone conformation in horses, yet validation of these parameters in foals has not previously been undertaken. This study aimed to determine the intra- and inter-rater repeatability and reproducibility of established carpal and third metacarpal (MC3) radiographic parameters in Thoroughbred foals and to establish baseline conformational data. Twenty Thoroughbred foals were radiographed without sedation from birth to 14 months of age at two-month intervals. Dorsopalmar carpal and lateromedial MC3 radiographs were obtained using sta...
New Insights in Heart Rate Variability Through Non-Linear Methods for Conditioning Assessment in Horses.
Veterinary journal (London, England : 1997)    May 26, 2026   106722 doi: 10.1016/j.tvjl.2026.106722
Vieira Ramos G, Camargo Ferraz G, Fazan R, Virgilio Silva LE, de Lacerda-Neto JC.Chronic exercise induces significant adaptations across multiple physiological systems due to increased metabolic demand. Heart rate variability (HRV) has emerged as a prominent, non-invasive method for monitoring fitness, enabling inference of sympathovagal balance from time-series variations in electrocardiograms. Notably, nonlinear analyses have a strong capacity to detect and estimate complex mechanisms underlying cardiovascular modulation. In this context, HRV was evaluated in the time and frequency domains, as well as through nonlinear methods, in 12 young purebred Arabian horses undergo...
Characterization of Capillary Zone Electrophoresis in Horses and Donkeys.
Veterinary sciences    May 26, 2026   Volume 13, Issue 6 513 doi: 10.3390/vetsci13060513
Davias C, Mendoza FJ, Heras AL, Perez-Ecija A.There are no capillary zone electrophoresis (CZE) comparative studies between horses and donkeys. Our objectives were to compare CZE results between both species, establish reference intervals (Ris), study age- and sex-related changes, determine the prevalence of bisalbuminemia, and correlate spectrophotometric albumin results with those calculated based on two different CZE methods. We performed CZE in 50 healthy horses and 49 healthy donkeys. There were significant differences ( < 0.03) in every protein fraction percentage and concentration (except for the β-globulins percentage) between...
An epizootic of equine viral arteritis at a Thoroughbred racetrack in Illinois: 223 cases. Foreman JH, Scollay MC.An epizootic of equine viral arteritis (EVA) occurred over an 8-wk period in the summer of 1993 at a midwestern Thoroughbred (TB) racetrack that ceased operations in 2021. Based on clinical signs, viral isolation, and serology, 223 horses (213 TBs, 10 lead ponies) were affected. Consistent clinical signs included fever (38.3-40.6°C; 101-105°F), depression, inappetence, and diffuse urticaria, including distal limb and preputial edema. Equine arteritis virus (EAV) was cultured from buffy coat samples from 24 of 28 acutely ill horses. Seventeen of 18 convalescent horses and 1 of 92 non-affected...
Comparative analysis of mRNA and circRNA expression profiles and interaction networks in testicular tissues of Mongolian horses before and after sexual maturity.
BMC genomics    April 28, 2026   doi: 10.1186/s12864-026-12868-8
Du M, Liu Y, He Q, Zhang X, Zhao Y, Li B, Bai D, Bou G, Bao T, Wen X, Dugarjaviin M.Mongolian horses, celebrated for their resilience and adaptability, undergo marked physiological transformations during sexual maturation. This study employed whole-transcriptome sequencing to conduct a comparative analysis of mRNA and circular RNA (circRNA) expression profiles, along with their interactive regulatory networks, in testicular tissues of Mongolian horses pre- and post-sexual maturity. Histological examinations using hematoxylin-eosin (H.E.) and 4’,6-diamidino-2-phenylindole (DAPI) staining revealed distinct morphological differences between sexually immature and mature testicu...
Pasture Intake of Whole-Day Stocked Thoroughbred Foals With Growth.
Animal science journal = Nihon chikusan Gakkaiho    April 28, 2026   Volume 97, Issue 1 e70168 doi: 10.1111/asj.70168
Tanabe T, Matsui A, Tominari M, Ueda K, Mitani T, Kawai M.We investigated the changes in the forage and nutrient intake of thoroughbred foals on pastures with whole-day stocking with age. Twelve thoroughbred foals were used and were allowed to graze on a pasture for 21 h/day from June to September. All foals were weaned just before 5 months of age. The pasture intake of the foals was measured using the double-indicator method. During the suckling period, the total voluntary dry matter intake (VDMI) of the pasture for foals was higher at 4 months (2.1 kgDM/day) than at 2 months (1.3 kgDM/day). However, VDMI of pastures per body weight (%BW) for foal...
Transcriptome Sequencing and Differential Analysis of Testes in One- and Two-Year-Old Kazakh Horses.
Animals : an open access journal from MDPI    April 16, 2026   Volume 16, Issue 8 1220 doi: 10.3390/ani16081220
Su Y, Wen L, Jiang J, Wen M, Zeng Y, Meng J, Wang J, Ren W, Yao X.This study systematically elucidated the developmental characteristics and molecular regulatory mechanisms of the testis during the critical period of sexual maturation in Kazakh horses by combining histological observation of one- and two-year-old testicular tissues with transcriptomic sequencing. In the testes of one-year-old horses, no obvious lumen was observed, and the interior is mainly comprising supporting cells and spermatogonia on the basement membrane; in contrast, in the testes of two-year-old horses, the tubular lumen was complete with spermatogonia, spermatocytes, and spermatozoa...
Adrenocorticotropic Hormone and Thyroxine Concentrations in Young Healthy Equids: Species Differences and Seasonal Variations.
Animals : an open access journal from MDPI    April 7, 2026   Volume 16, Issue 7 1124 doi: 10.3390/ani16071124
Siwińska N, Żak-Bochenek A, Jawor P, Pietrzak A, Kutermak O, Słowikowska-Łoś M, Kaczmarek B.Due to growing interest in endocrine diseases, more studies aim to interpret the baseline concentrations and reference intervals of hormone values like adrenocorticotropic hormone (ACTH) and thyroxine (T4) in horses. Most studies available in the literature focus on evaluating ACTH and thyroid hormone values in adult and geriatric horses, with only a few considering younger donkeys and ponies. This study aimed to measure ACTH and T4 in autumn and spring in younger equids. The study included 42 young equids, which were divided into three groups: horses, ponies, and donkeys. The concentration of...
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