Analyze Diet

Topic:Statistical Analysis

Statistical analysis in equine research involves the application of mathematical techniques to collect, review, and interpret data related to horse health, behavior, and performance. This field encompasses a variety of methods, including descriptive statistics, inferential statistics, and multivariate analysis, to identify patterns, test hypotheses, and draw conclusions from equine data. Statistical analysis aids in understanding various aspects of equine science, such as disease prevalence, treatment efficacy, genetic traits, and performance metrics. This page aggregates peer-reviewed research studies and scholarly articles that explore the methodologies, applications, and outcomes of statistical analysis in the context of equine studies.
Impact of collagen supplementation on the fecal microbiome of young horses in training.
Journal of equine veterinary science    June 6, 2026   106047 doi: 10.1016/j.jevs.2026.106047
Júnior AMCA, da Silva AH, Buroxid RP, Padovan JR, Nunes AT, Poleti MD, Fukumasu H, Gobesso AAO.Intense training in athletic foals may alter fecal microbiota, highlighting the need for nutritional strategies such as hydrolyzed collagen supplementation. Objective: This study aimed to evaluate the effects of dietary hydrolyzed collagen on the fecal microbiome of weanlings in training. Methods: Twenty Mangalarga Marchador foals (206 ± 18 kg; 7 ± 1 mo age) were assigned to two groups: a supplemented group (50 g/day of hydrolyzed collagen for 180 days) and a control group. A completely randomized design with repeated measures over time was used. Foals were exercised five consecutive days pe...
Thermal Thresholds and Genetic Sensitivity in Barrel Racing Performance of Quarter Horses Across Temperature-Humidity Indices. Santana ML, Bignardi AB.Heat stress is an increasingly important challenge for the performance and welfare of equine athletes, particularly in competitions conducted across diverse climatic conditions. Understanding the genetic basis of performance responses to increasing thermal load is therefore essential to support robust genetic evaluation and sustainable selection strategies. The objective of this study was to evaluate barrel racing performance of Brazilian Quarter Horses across thermal environments defined by two widely used indicators, the temperature-humidity index (THI) and the wet bulb globe temperature (WB...
Embryo developmental kinetics and vitrification in relation to pregnancy outcome, foaling percentage and foal sex.
Equine veterinary journal    May 31, 2026   doi: 10.1002/evj.70197
Peere S, Dewulf M, Van den Branden E, Papas M, Broothaers K, De Coster T, Briote I, Hedia M, Angel-Velez D, Hoogewijs M, Govaere J, Smits K.While fast in vitro embryo development has been associated with improved outcomes, the relationships of slow in vitro embryo development and vitrified versus fresh in vitro produced (IVP) embryo transfer to foaling percentage and foal sex are less explored. Objective: To determine the relationships of (1) day of blastocyst formation (D7-11 after intracytoplasmic sperm injection (ICSI)) and (2) vitrification and warming method (one- and three-step) versus fresh transfer with pregnancy, early pregnancy loss (EPL), foaling percentage and foal sex. Methods: Retrospective clinical study. Methods: B...
Thoracic electrical impedance tomography using a two-plane electrode configuration in horses.
Equine veterinary journal    May 28, 2026   doi: 10.1002/evj.70188
Studer N, Byrne D, Raisis A, Adler A, Cheong J, Secombe C, Schramel JP, Mosing M.Traditional one-plane (oneP) electrical impedance tomography (EIT) electrode configurations limit evaluation to a convex lens-shaped lung slice, prone to overrepresentation of central areas. Two-plane (twoP) EIT is expected to refine this technique and result in more representative lung imaging. Objective: To compare ventilation distribution using single slice reconstructions, obtained with twoP or oneP. Methods: Computational modelling and in vivo exploratory randomised experimental trial. Methods: A finite element simulation model of oneP and twoP was calculated to estimate captured lung fie...
Is the walk and trot efficiency a hereditary trait in primitive Konik Polski horses?
PeerJ    May 27, 2026   Volume 14 e21369 doi: 10.7717/peerj.21369
Siemieniuch-Tartanus M, Tomczyk-Wrona I, Tobolski D.Performance testing is a critical tool for achieving genetic progress, yet its long-term effectiveness in primitive breeds under conservation programs is poorly documented. The Konik Polski horse breeding program aims to improve utility while preserving primitive characteristics. Unassigned: This retrospective study analyzed a 25-year longitudinal dataset ( = 1,608) from the official Field Performance Test to quantify genetic trends in gait efficiency, using nonparametric methods to assess the effects of test type, year, and age on stride length and speed at the walk and trot. To estimate he...
Outcomes and racing performance in thoroughbreds following a long-term injury sustained during a jump race in Great Britain.
The Veterinary record    May 26, 2026   doi: 10.1002/vetr.70807
McDonald S, Verheyen KLP, Chang YM, Allen SE.Limited literature is available about the career outcomes of horses sustaining a long-term injury (LTI) in a jump race. Methods: All Thoroughbreds that sustained an LTI, defined as any non-fatal musculoskeletal injury requiring a minimum 90-day break from racing, in a British jump race between May 2018 and April 2023 were followed up for at least 24 months and outcomes described. Pre-injury racing performance was compared between horses that did and did not return to racing, and post-injury racing performance was compared between horses that sustained tendon/ligament injuries and fractures. R...
Evaluation of Circadian Variations of Central Corneal Thickness and Its Correlation With Intraocular Pressures in Healthy Horses.
Veterinary ophthalmology    May 20, 2026   Volume 29, Issue 4 e70194 doi: 10.1111/vop.70194
Iturregui C, Liu CC, Carter RT, Camacho-Luna P.Circadian variations in central corneal thickness (CCT) and their correlation with intraocular pressure (IOP) have not been reported in horses. This information may be clinically relevant when interpreting IOP readings. Methods: Twenty-four eyes of 6 healthy adult gelded males and 6 healthy adult intact mares were evaluated. Rebound tonometry (TonoVet, Jorgensen Laboratories) followed by ultrasonic pachymetry (iPac Pachymeter, Reichert technologies) was performed in both eyes every 3 h over 24 h during the natural 14-h daylight summer photoperiod. Results: Central corneal thickness varied ...
Genetic Diversity and Population Structure in Seven Lipizzan Populations Based on Microsatellite Genotyping.
Animals : an open access journal from MDPI    May 15, 2026   Volume 16, Issue 10 1516 doi: 10.3390/ani16101516
Rogić B, Moravčíková N, Margeta P, Štrbac L, Zorc M, Kovács M, Vostry L, Kasarda R, Posta J.Lipizzan horses, bred for over four centuries, represent a unique genetic, cultural and historic resource. This study assessed genetic diversity and population structure across seven European Lipizzan populations, with emphasis on the Bosnia and Herzegovina population. A total of 547 Lipizzan horses were genotyped using 12 microsatellite markers. The parameters of genetic variability, admixture level and migration rate were calculated. The mean number of alleles, effective number of alleles and Shannon's information index were 5.78, 3.24 and 1.31, respectively. The average fixation indices wer...
A Comparison of the Performance of Horses from the International WBFSH Rankings in Dressage, Jumping and Eventing Based on Their Sex, Age, Proportion of Thoroughbred Genes and Affiliation to the Studbook.
Animals : an open access journal from MDPI    May 14, 2026   Volume 16, Issue 10 1509 doi: 10.3390/ani16101509
Sobotková E, Kuřitková D, Zeliszewska-Duk P, Mikule V.The aim of the research was to assess how the selected factors could influence the performance of the best horses in dressage, jumping and eventing competitions. The research was based on the Studbook Rankings published by the World Breeding Federation for Sport Horses from 2019 to 2024. Our analysis shows a statistically significant effect of each of the observed factors in at least one discipline. The leading factor in all disciplines was studbook affiliation. The Belgian warmblood, German Sport Horse, Hanoverian, Royal Dutch Sport Horse, Oldenburg and Westphalian studbooks are represented a...
Unraveling the genomic architecture of hock conformation defects in Pura Raza Española horses.
Journal of animal science    May 13, 2026   skag116 doi: 10.1093/jas/skag116
Laseca N, Molina A, Ziadi C, Perdomo-Gonzalez DI, Valera M.Conformational traits define a horse's shape and morphology, heavily influencing its functionality, sport performance, health, and appearance, making conformation a critical selection criterion in horse breeding. However, the genomic basis of conformational defects, specifically those affecting the hock, remains largely unexplored. This research aimed to identify the genomic regions associated with hock defects using a large cohort of Pura Raza Española (PRE) horses. A total of 58,930 horses were evaluated for hock angle, with 4,122 genotyped on high or medium density arrays. For this purpose...
Size of the C7-T1 articular processes is overestimated on lateral radiographs when compared to standing cone beam computed tomography.
Equine veterinary journal    May 13, 2026   doi: 10.1002/evj.70184
Cianci JM, Bills KW, Skelton G, Stewart HL, Johnson AL, Colmer SF, Stefanovski D, Brown KA.Radiography of the caudal cervical vertebral column is commonly performed in horses, but image distortion may occur due to factors such as scapular superimposition, divergent beam, and the distance between anatomy and detector. These variables can falsely suggest enlargement of the articular processes (APs) at the seventh cervical and first thoracic vertebrae (C7-T1). Objective: To assess the presence of magnification of C7-T1 APs as determined by AP:vertebral body (VB) height ratio on lateral radiographs compared to cone beam computed tomography (CBCT) as a gold standard, and to explore inter...
From Concept to Perception: Equestrian Definitions of Harmony and Visual Attention in Horse-Rider Evaluation.
Animals : an open access journal from MDPI    May 12, 2026   Volume 16, Issue 10 1483 doi: 10.3390/ani16101483
Wolframm IA, Everding M, Savulchyk V, Borssen J, Gudden DDM.In equestrian circles, horse-rider harmony is understood intuitively, yet clear criteria are lacking. This study examined how different equestrians conceptualized and visually assessed harmony and how this influenced scoring. Qualitative interviews were combined with eye tracking technology. Thirty equestrians assessed five videos of horse-rider combinations performing in dressage, showjumping, eventing, working equitation and Icelandic riding, with eye movements being recorded using a mounted eye tracker. Verbal definitions were analyzed using thematic analysis, revealing three overarching th...
Air pollution exposure during training impairs performance in Thoroughbred racehorses.
Equine veterinary journal    May 10, 2026   doi: 10.1002/evj.70186
Scott D, Seabaugh K, Kim L, Magzamen S, Duncan C.Ambient air pollution contributes substantially to human morbidity and mortality, and athletes are recognised as a particularly vulnerable group. However, little is known about the impact of air pollution on equine athletes. Objective: To explore the relationship between air pollution exposure during the pre-competition training period and race day performance among Thoroughbred racehorses that competed on California racetracks. Methods: A retrospective longitudinal study. Methods: For each winning horse, pollutant exposure during the 21-day pre-competition training period was assigned using d...
Inter-individual variability in equine antibody responses to African snake venoms follows heavy-tailed distributions with implications for antivenom production.
Toxicon : official journal of the International Society on Toxinology    May 6, 2026   109145 doi: 10.1016/j.toxicon.2026.109145
Sánchez A, Sánchez P, Sánchez A, Durán G, Solano G, Villalta M, Gómez A, Gutiérrez JM, León G.Variability in the antibody response of horses used for snake antivenom manufacture is well recognized, yet its statistical structure and implications for industrial productivity remain poorly characterized. In this study, we quantified antivenom antibody titers by ELISA in a cohort of 14 horses immunized with venoms from the clinically most important snakes in sub-Saharan Africa. To integrate antibody levels with plasma availability, we calculated the Cumulative Plasma Productivity (CPP) by converting individual plasma volumes into titer-corrected equivalents and sequentially pooling these vo...
Global prevalence and epidemiological determinants of African horse sickness: A systematic review and meta-analysis.
Veterinary journal (London, England : 1997)    May 4, 2026   106698 doi: 10.1016/j.tvjl.2026.106698
Purnama MTE, Firdausy LW, Fikri F, Wicaksono AP, Maslamama ST, Wardhani LDK.African horse sickness (AHS) is a highly fatal, vector-borne viral disease of equids with substantial economic and animal health impacts, particularly in endemic regions. Despite numerous epidemiological studies, reported prevalence estimates remain highly variable, and a comprehensive quantitative synthesis is lacking. This study aimed to systematically evaluate and synthesize the global prevalence of AHS and to identify sources of epidemiological heterogeneity. A systematic review and meta-analysis were conducted following PRISMA 2020 guidelines, with the protocol registered in the Open Scie...
Influence of Housing and Fecal Egg Count on Cytokine Profiles (IL-4 and IL-13) in Equines.
Journal of equine veterinary science    May 1, 2026   105922 doi: 10.1016/j.jevs.2026.105922
Yalavarthi G, Sabbinen H, Kosuri P, Amalaradjou M, Govoni K, Nadeau J.Strongyles are parasitic roundworms that cause significant gastrointestinal infections in equines, primarily through contact with infected soil. Worldwide, strongylosis affects nearly 90% of horse populations. Objective: The objectives of our study were to determine if there was a relationship between fecal egg count (FEC) and housing types on interleukin IL-4 and IL-13. Methods: Horses (N=25) were separated into two groups based on housing conditions: pasture (housed on pasture 24/7; PS, N=13) and semi-intensive (housed in stalls with turnout overnight; SI, N=12). Within each housing group, t...
Allogeneic Platelet-Rich Gel Supernatant Reprograms the Cytokine and Growth Factor Microenvironment in an Equine In Vitro Inflammatory Tendon System.
International journal of molecular sciences    April 29, 2026   Volume 27, Issue 9 4006 doi: 10.3390/ijms27094006
Carmona JU, López C.Tendinopathy involves a dysregulated inflammatory microenvironment in which cytokines, growth factors (GF) and extracellular matrix components interact dynamically. Platelet-rich plasma (PRP) is widely used as a regenerative therapy, but its mechanisms of action in inflamed tendon remain unclear. This study evaluated whether platelet-rich gel supernatant (PRGS) reprograms the inflammatory-anabolic mediator network in an equine in vitro tendon explant system stimulated with lipopolysaccharide (LPS). Tendon explants were cultured under six experimental conditions, including non-stimulated contro...
Machine Learning-Based Genome-Wide Association Study Reveals Genetic Loci Associated with Body Measurement Traits in Yili Horses.
Animals : an open access journal from MDPI    April 29, 2026   Volume 16, Issue 9 1373 doi: 10.3390/ani16091373
Shen Z, Yang L, Xue Y, Chang X, Shen J, Sun W, Zeng Y, Meng J, Yao X.Body measurement traits are key indicators for evaluating growth performance, production potential, and breeding value in Yili horses. However, studies investigating the association between body measurement traits and mutation loci in Yili horses remain limited. In this study, 255 adult Yili mares were used as the study population, including 152 speed-type and 103 meat-type individuals. Whole-genome resequencing was performed, and four phenotypic traits and body weight were measured. A mixed linear model (MLM)-based genome-wide association study (GWAS) was conducted using GEMMA (v 0.98.5), inc...
The incidence of fatal and non-fatal injury in thoroughbred and Quarter Horse racehorses in Louisiana (2014-2022).
Research in veterinary science    April 27, 2026   Volume 207 106219 doi: 10.1016/j.rvsc.2026.106219
Dirikolu L, Liu CC, Schaeffer DJ.The objectives of the study were to report the incidence of race-related injuries and equine fatalities in racehorses at the Louisiana racetracks from 2014 to 2022 for both Thoroughbred and Quarter Horse racehorses. Additionally, this study evaluated several factors that may be related to the occurrence of race-related injuries in racehorses in Louisiana. The average rates of equine fatal injuries (euthanized on or off track within 72 h of the race) were 1.70 ± 0.76 and 1.94 ± 0.89 per 1000 starts in Thoroughbred and Quarter racehorses, respectively. The average rates of non-fatal injur...
Effects of shoeing on forelimb biomechanics in walking horses.
Equine veterinary journal    April 26, 2026   doi: 10.1002/evj.70182
Wang JY, Guan S, Walmsley E, Wells D, Pandy MG, Whitton C.Hoof trimming and shoeing techniques are used to manage and prevent equine limb injuries. However, quantitative studies comparing the effects of different shoeing techniques on equine joint biomechanics over the full gait cycle are lacking. Objective: To measure and compare joint motion and net torques at the distal forelimb joints when horses walk overground unshod, with a standard flat shoe, and with a rocker shoe. Methods: In vivo study. Methods: Gait data were recorded from 12 sound horses during walking. Three shoeing conditions were tested: unshod, flat shoe, and rocker shoe. Data were r...
Epidemiology of open and closed diastemata in equine cheek teeth and associations with periodontal pathology.
Veterinary research communications    April 23, 2026   Volume 50, Issue 4 280 doi: 10.1007/s11259-026-11216-1
Mata F, Johnson C.Cheek-tooth diastemata are a common dental abnormality in horses and are considered a major predisposing factor for periodontal disease. However, the influence of diastema type and anatomical location on disease severity remains incompletely understood. To determine the prevalence and distribution of open and closed cheek-tooth diastemata and evaluate their association with periodontal disease severity in horses, while assessing the influence of age, sex, jaw location and laterality, fifty equine cadaver heads obtained from an abattoir were examined. Diastemata were identified through visual a...
Impact of evidence-based information on horse owners’ misconceptions of colic.
Equine veterinary journal    April 22, 2026   doi: 10.1002/evj.70170
Burrell KL, England GCW, Burford JH, Freeman SL.Misconceptions can be defined as 'false, persistent beliefs' or 'inaccurate, prior knowledge' and can influence decision-making. Objective: To investigate the impact of evidence-based information on UK horse owners' decision-making for colic. Methods: Mixed-methods cross-sectional study. Methods: An online survey was distributed to UK horse owners, with four sections: owner demographics; views on decision-making and referral to an equine hospital facility for colic; current knowledge and approach to colic; impact of evidence-based information (including evidence on recognising and responding t...
Audio-Based Characterization of Gait Parameters in Mangalarga Marchador, Campolina, and Piquira Horses Using Deep Learning.
Animals : an open access journal from MDPI    April 22, 2026   Volume 16, Issue 9 1283 doi: 10.3390/ani16091283
Freire A, Silva AVD, Patterson Rosa L, Guimarães PHS, Araujo BPG, Silva CAF, Borges LRT, Bertechini AG, Meirelles SLC.The evaluation of biomechanical parameters in four-beat gaited horses remains limited by the subjectiveness and complexity of current standard methods. Through a deep learning approach, we aimed to infer dissociation % using only acoustic signals. A total of 268 audio samples were extracted from publicly available videos featuring three Brazilian horse breeds (Mangalarga Marchador, Campolina, and Piquira) performing marcha batida and marcha picada. Acoustic features, including root mean square energy (RMS), zero-crossing rate (ZCR), and 13 Mel-frequency cepstral coefficients (MFCCs), were extr...
ProAKAP4 as a predictor of semen freezability in Asturcon ponies.
Journal of equine veterinary science    April 22, 2026   Volume 162 105912 doi: 10.1016/j.jevs.2026.105912
Cryopreservation is a valuable tool in equine reproduction, although its success remains highly variable among stallions. The proAKAP4 protein has been proposed as a molecular marker of sperm quality and cryotolerance. Objective: This study aimed to evaluate the relationship between proAKAP4 concentration and sperm cryotolerance in ejaculates from Asturcon ponies, and to assess whether proAKAP4 levels in fresh semen can predict post-thaw sperm quality parameters before and after cryopreservation. Methods: Twenty-four ejaculates from eight different stallions were used (three ejaculates per ani...
Genomic diversity and structure in arabian horses revealed by whole-genome sequencing: establishment of an allele frequency database of common genetic variation.
BMC genomics    April 20, 2026   doi: 10.1186/s12864-026-12862-0
Szmatoła T, Finno C, Gurgul A, Heath H, Stefaniuk-Szmukier M, Almarzook S, Norton E, Ropka-Molik K.No abstract available
Extraction of Kinematic Parameters and Comparative Study of Endurance Levels in Mongolian Horses.
Veterinary sciences    April 20, 2026   Volume 13, Issue 4 404 doi: 10.3390/vetsci13040404
Shen Y, Su L, Zhang Y, Liu J, Zhang Z, Zhang S.Mongolian horses are an indigenous Chinese breed known for their endurance capacity, yet quantitative descriptions of their gait-related kinematic characteristics remain limited. This pilot exploratory study aimed to describe the kinematics of Mongolian horses during walk, slow trot, and fast trot, and to examine whether selected variables differed between race-result groups in a 12 km endurance race. Forty-six horses were classified into an excellent group and an ordinary group based on the result of a single race. Kinematic data were collected using optical motion capture and three-dimension...
Age-Associated Differences in Paddock Locomotor Activity Among Senior Horses: A Pilot Observational Study.
Animals : an open access journal from MDPI    April 15, 2026   Volume 16, Issue 8 1208 doi: 10.3390/ani16081208
Poinsard L, Anson C, Billat V.Turnout locomotor activity is a potentially informative indicator of health and welfare in older horses, yet objective field data in seniors remain limited. We examined whether a brief turnout recording could detect cross-sectional associations between chronological age and locomotor activity in senior horses in this study setting. In this single-site observational study, 28 senior Selle Français horses (17-35 years) contributed 122 paddock sessions (2 h each), with total distance and mean speed quantified using a Polar Team Pro sensor. Associations with age were assessed using linear mixed-e...
Morphometric Characterization and Preliminary Heritability Estimates of Body Measurements in the Polish Konik Populations.
Animals : an open access journal from MDPI    April 14, 2026   Volume 16, Issue 8 1190 doi: 10.3390/ani16081190
Pasicka E, Sobek Z, Wierzbicki H, Różańska-Zawieja J, Nienartowicz-Zdrojewska A.The objective of this paper is to demonstrate the morphometric diversity of 172 Polish Koniks from the largest Polish breeding centers and to assess their genetic diversity by estimating the effective population size () and heritabilities () of the studied traits. The animals were bred under a stable housing system. Horses of both sexes aged 3 to 24 years were analyzed. Each horse was characterized with 40 metric traits. Multifactorial analyses (PCA, DA) and the REML method were applied to reveal the exterior differentiation and the development of distinct morphometric types. The findings esta...
Genetic diversity in the Criollo Argentino horse from SNP array data.
Genetica    April 11, 2026   Volume 154, Issue 1 16 doi: 10.1007/s10709-026-00266-7
No abstract available
Haplotype GWAS in swedish warmblood horses for conformation and jumping traits.
Journal of animal science    April 10, 2026   skag109 doi: 10.1093/jas/skag109
Ablondi M, Eriksson S, Gelinder Viklund Å, Mikko S.The aim of this study was to better understand the genomic architecture behind performance-related traits in sport horses. In this study, we conducted a haplotype-based genome-wide association study (GWAS) for 36 conformation and free jumping phenotypes recorded during routinely conducted young horse evaluation tests involving 380 Swedish Warmblood (SWB) horses. The horses were evaluated by expert judges using both traditional and linear evaluation systems. All samples were genotyped using the 670K Affymetrix® Axiom® Equine Genotyping Array, haplotypes were first phased, and haplotype blocks...
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