In vitro equine oocyte maturation in pure follicular fluid plus interleukin-1 and fertilization following ICSI.
Abstract: The interleukin-1 (IL-1) system is thought to be involved in periovulatory events in the mare. Previous in vivo studies have demonstrated that IL-1beta induces oocyte maturation, but depresses the pregnancy rate 14 days after ovulation. To better understand the role of IL-1 in oocyte maturation and fertilization, the effects of IL-1 on the in vitro maturation rate of equine oocytes in pure follicular fluid were evaluated and fertilization rate assessed following intracytoplasmic sperm injection (ICSI). Oocytes collected from slaughterhouse ovaries were cultured in four different media for 30 h prior to fertilization. Two experiments were performed, each using three maturation media as the experimental treatments. Medium 1 was pure follicular fluid from subordinate follicles. Medium 2 was medium 1 plus 50 ng/ml recombinant human IL-1beta. Medium 3 was pure follicular fluid collected from mares administered crude equine gonadotropin (CEG). Medium 4 was medium 2 plus 50 ng/ml of recombinant human IL-1 receptor antagonist. Media 1, 2 and 3 were compared in experiment 1. In experiment 2, media 1, 2 and 4 were compared. After maturation, metaphase II oocytes were submitted to microinjection and assessed for signs of fertilization. In experiment 1, 101 oocytes were evaluated. The rate of polar body extrusion was 66, 51 and 68% and the proportions of normally fertilized oocytes after ICSI were 40, 18 and 38% for media 1, 2 and 3, respectively. In experiment 2, 122 oocytes were evaluated. The rate of polar body extrusion was 55, 48 and 42% and the proportions showing normal fertilization after ICSI were 14, 25 and 29% for media 1, 2 and 4, respectively. There was no positive effect of IL-1beta on maturation in both experiments, but the fertilization rate and percentage of embryos reaching four-cell were low in the presence of IL-1beta, indicating that this cytokine may interfere with fertilization and early embryo development.
Publication Date: 2007-06-13 PubMed ID: 17628364DOI: 10.1016/j.anireprosci.2007.06.005Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates the role of interleukin-1 (IL-1) in oocyte maturation and fertilization in horses using in vitro tests. Findings suggest that the presence of IL-1 may interfere with the fertilization process and early embryo development.
Research Objectives and Methodology
- The goal of this study was to understand the role of interleukin-1 (IL-1) in oocyte (egg cell) maturation and fertilization in equine (horse).
- Earlier studies have suggested that IL-1 plays a role in periovulatory events in horses. However, a clear understanding of this association is not yet drawn.
- Researchers evaluated the impact of IL-1 on the maturation rate of horse oocytes in pure follicular fluid and also assessed the fertilization rate following intracytoplasmic sperm injection (ICSI).
- Oocytes were taken from horse ovaries from a slaughterhouse and were cultured in four different media for a period of 30 hours before fertilization. In two experiments, three maturation media were compared using different combinations.
Experiments and Findings
- In experiment 1, 101 oocytes were assessed. Comparisons were made between a medium of pure follicular fluid from subordinate follicles (medium 1), medium 1 plus 50 ng/ml recombinant human IL-1beta (medium 2), and pure follicular fluid collected from mares given crude equine gonadotropin (medium 3).
- The results of experiment 1 showed that the rate of polar body extrusion – a marker of oocyte maturation, was 66%, 51%, and 68% and normally fertilized oocytes after ICSI were 40%, 18%, and 38% for media 1, 2 and 3 respectively.
- In experiment 2, 122 oocytes were evaluated. The treatments used for comparison were the same as in the first experiment, but medium 4 was introduced, which was medium 2 plus 50 ng/ml of recombinant human IL-1 receptor antagonist.
- The results of experiment 2 showed that the rate of polar body extrusion was 55%, 48%, and 42% while the proportions showing normal fertilization after ICSI were 14%, 25%, and 29% for media 1, 2, and 4, respectively.
- In both experiments, IL-1beta did not enhance maturation, but the fertilization rate and percentage of embryos reaching four-cell were low in its presence, suggesting that IL-1beta may interfere with fertilization and early embryo development.
Cite This Article
APA
Caillaud M, Dell'aquila ME, De Santis T, Nicassio M, Lacalandra GM, Goudet G, Gérard N.
(2007).
In vitro equine oocyte maturation in pure follicular fluid plus interleukin-1 and fertilization following ICSI.
Anim Reprod Sci, 106(3-4), 431-439.
https://doi.org/10.1016/j.anireprosci.2007.06.005 Publication
Researcher Affiliations
- INRA, CNRS, Université de Tours, Haras Nationaux, IFR 135, 37380 Nouzilly, France. maudcaillaud@gmail.com
MeSH Terms
- Animals
- Cells, Cultured
- Culture Media / pharmacology
- Embryo, Mammalian / cytology
- Female
- Fertilization / drug effects
- Follicular Fluid / physiology
- Horses / physiology
- Interleukin-1beta / pharmacology
- Oocytes / drug effects
- Oocytes / physiology
- Oogenesis / drug effects
- Pregnancy
- Pregnancy Rate
- Pregnancy, Animal
- Sperm Injections, Intracytoplasmic / drug effects
Citations
This article has been cited 2 times.- Satué K, Fazio E, Medica P. Can the Presence of Ovarian Corpus Luteum Modify the Hormonal Composition of Follicular Fluid in Mares?. Animals (Basel) 2020 Apr 9;10(4).
- Al-Mutary M, Al-Ghadi M, Al-Himaidi A, Iwamoto D, Al-Anazi Y, Ammari A, Ahmad J, Al-Khedhairy A. Using RT-PCR and glutathione level to study the effect of follicular fluid on in vitro maturation and gene expression of sheep oocytes. Saudi J Biol Sci 2019 Sep;26(6):1216-1222.
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