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Reproductive biology and endocrinology : RB&E2005; 3; 26; doi: 10.1186/1477-7827-3-26

In vivo effect of interleukin-1beta and interleukin-1RA on oocyte cytoplasmic maturation, ovulation, and early embryonic development in the mare.

Abstract: A growing body of evidence suggests that the interleukin-1 system is involved in periovulatory events. Previous work from our lab demonstrated that in the mare, interleukin-1beta (IL-1beta) increases the ovulatory rate of metaphase II oocytes. The present study was conducted to analyze in vivo the effect of IL-1 on oocyte cytoplasmic maturation, ovulation and pregnancy rate. In the present work, IL-1beta (experiment 1, n = 13; experiment 2, n = 25) and interleukin-1RA (IL-1RA; experiment 1, n = 25) were injected intrafollicularly by using the transvaginal ultrasound-guided injection method. Injections were performed on cyclic mares when the diameter of the growing dominant follicle reached 30-34 mm. In experiment 1, mares were inseminated the day of the treatment and all the other day until ovulation. The time of ovulation was determined and a pregnancy diagnosis was performed 14 days after ovulation of the injected follicle. In experiment 2, the cumulus-oocyte complex from each injected follicle was collected by transvaginal ultrasound-guided aspiration 38 h after the intrafollicular injection. Oocyte nuclear stage and oocyte cytoplasmic maturation were assessed by analyzing chromatin configuration, cortical granules migration and mitochondria distribution under a confocal microscope. The results from experiment 1 confirm that an intrafollicular injection of 1 microgram IL-1beta induces ovulation in the mare whereas IL-1RA has no effect at the dose used in the present study. Furthemore, we demonstrated, that in our experimental conditions, IL-1beta and IL-1RA induced a decrease in embryo development. Experiment 2 leads us to observe that IL-1beta is unable to induce cortical granules migration and remodelling of mitochondria, that commonly occurs during oocyte maturation, whereas it acts on nuclear maturation. This result may explain the decrease in embryo development we observed after IL-1beta intrafollicular injection. In conclusion, the present study tends to demonstrate that IL-1beta plays a role in the ovulatory process and may acts on oocyte maturation in the mare, but additional factors are required to complete equine oocyte cytoplasmic maturation to allow embryo development.
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Publication Date: 2005-06-22 PubMed ID: 15972098PubMed Central: PMC1182395DOI: 10.1186/1477-7827-3-26Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the role of interleukin-1beta (IL-1beta) and interleukin-1RA (IL-1RA) in oocyte maturation, ovulation, and early embryonic development in horses. The research found that while IL-1beta can induce ovulation and may impact oocyte maturation, both IL-1beta and IL-1RA led to a reduction in embryo development.

Objective and Methodology

  • The study aimed to evaluate the in vivo effect of IL-1beta and IL-1RA on the maturation of oocyte cytoplasm, the rate of ovulation, and the likely outcome of pregnancy in horses.
  • To achieve the research objectives, researchers intravenously injected IL-1beta and IL-1RA into the ovarian follicles of test horses. The diameters of the dominant follicle in these horses had reached between 30-34 mm at the time of injection.
  • The horses were inseminated the day of the treatment and every other day until ovulation. Pregnancy diagnosis was carried out 14 days after ovulation.
  • In the second experiment, researchers collected the cumulus-oocyte complex from each injected follicle 38 hours after injection. Analysis was undertaken to assess oocyte nuclear stage and cytoplasmic maturation.

Results

  • The study found that the injection of 1 microgram of IL-1beta leads to ovulation in the mare, while IL-1RA did not have an effect at the dosage used.
  • Interestingly, both IL-1beta and IL-1RA were found to decrease embryo development under the conditions of the experiments.
  • It was discovered that IL-1beta does not induce the migration of cortical granules and restructuring of mitochondria that typically occurs during oocyte maturation. It does, however, act on nuclear maturation.

Conclusions

  • The results suggest that IL-1beta plays a crucial role in the ovulation process and may influence oocyte maturation in horses.
  • However, neither IL-1beta nor IL-1RA alone can complete equine oocyte cytoplasmic maturation or promote embryo development.
  • This implies additional factors are required for these processes.

Cite This Article

APA
Caillaud M, Duchamp G, Gérard N. (2005). In vivo effect of interleukin-1beta and interleukin-1RA on oocyte cytoplasmic maturation, ovulation, and early embryonic development in the mare. Reprod Biol Endocrinol, 3, 26. https://doi.org/10.1186/1477-7827-3-26

Publication

Reproductive biology and endocrinology : RB&E
ISSN: 1477-7827
NlmUniqueID: 101153627
Country: England
Language: English
Volume: 3
Pages: 26

Researcher Affiliations

Caillaud, Maud
  • Physiologie de la Reproduction et des Comportements, INRA-CNRS-Université de Tours-Haras Nationaux, IFR135, 37380 Nouzilly, France. caillaud@tours.inra.fr
Duchamp, Guy
    Gérard, Nadine

      MeSH Terms

      • Animals
      • Female
      • Horses / embryology
      • Horses / physiology
      • Injections
      • Interleukin 1 Receptor Antagonist Protein
      • Interleukin-1 / pharmacology
      • Oocytes / drug effects
      • Oocytes / physiology
      • Ovarian Follicle / drug effects
      • Ovarian Follicle / physiology
      • Ovulation / drug effects
      • Pregnancy
      • Receptors, Interleukin-1 / antagonists & inhibitors
      • Sialoglycoproteins / pharmacology

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      This article has been cited 14 times.
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